Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK

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1 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK 05/09/2018 Cellular Pathology Level 2 Esher Wing Galsworthy Road Kingston upon Thames KT2 7QB Contact: Dr Sussan Gharaie Tel: +44 (0) Fax: +44 (0) sussan.gharaie@nhs.net Website: Testing performed at main address only Site activities performed away from the locations listed above: Location details Activity Location code Mortuary Galsworthy Road Kingston upon Thames KT2 7QB United Kingdom Mortuary Services N/A Assessment Manager: EWS Page 1 of 8

2 DETAIL OF ACCREDITATION Fixed, fresh and frozen tissue tissue sections Histological examinations for the purpose of clinical diagnosis Examination of tissues to identify or exclude morphological and cytological abnormalities for the purposes of clinical diagnosis. Routine staining Basophilic and eosinophilic structures Special Staining examinations for the purpose of assisting with clinical diagnosis Acid Mucopolysaccharide Acid and Neutral Mucopolysaccharide based on equipment manuals as relevant Specimen dissection and tissue processing using SOPs Dissection: DI-01, DI-03 to DI-15, DI-17 to DI-29, DI-30 Tissue processing - Leica ASP6025 using SOPs CU-45 Tissue embedding- Thermo Shandon Histo Centre using SOPs CU-13 Tissue sectioning- Leica microtomes using SOP ML-01 Cryotomy (production of frozen sections)- Leica cryostat using SOP CU-19 SOP ML-02 using Leica Autostainer XL with Haematoxylin and Eosin Frozen section staining SOPCU-19 Documented in-house procedures for manual special stains with reference to: Alcian Blue SOP SS-01 Alcian Blue/PAS SOP SS-02 Amyloid Elastic fibres and connective tissue Helicobacter Congo red SOP SS-04 Elastic Van Gieson SOP SS-07 Giemsa SOP SS-09 Assessment Manager: EWS Page 2 of 8

3 tissue sections Special Staining examinations for the purpose of assisting with clinical diagnosis Haematopoietic cells Myeloid cells Acid and neutral mucopolysaccharides Neutral mucopolysaccharides Reticulin fibres Gram positive and negative microorganisms Argyrophil cells Fungal cells Muscle and fibrin tissue Acids mucins Basement cell membranes Connective tissue Melanin Connective tissue Neutral mucins Haemosiderin Hepatitis B affected cells and copper associated protein Mast Cells Documented in-house procedures for manual special stains with reference to: Giemsa SOP SS-17 Chloroacetate Esterase SOP SS-03 Diastase/Alcian Blue/PAS SOP SS-05 Diastase/PAS SOP SS-06 Gordon and Sweets Retic SOP SS-10 Gram SOP SS-11 Grimelius SOP SS-12 Grocott SOP SS 13 Haematoxylin Van Gieson SOP SS-28 Hale s Dialysed Iron SOP SS-30 Jones Silver SOP SS-14 Marius Scarlet Blue SOP SS-15 Masson Fontana SOP SS-16 Masson Trichrome SOP SS-32 Periodic Acid Schiff SOP SS-21 Perls SOP SS-22 Shikata (Orcein) SOP SS-23 Toluidine Blue SOP SS-24 Assessment Manager: EWS Page 3 of 8

4 tissue sections tissue sections Special Staining examinations for the purpose of assisting with clinical diagnosis Calcium slats Mycobacterium leprae Spirochetes Mycobacterium species Immunohistochemical examinations for the purpose of clinical diagnosis Cytokeratins High molecular weight Cytokeratins B cells in the mantle zone and interfollicular T cells B cells not in the mantle zone Epithelial cells Tumour cells of stomach, small intestine, colon, liver and pancreas High weight glycoprotein Calcium binding protein Documented in-house procedures for manual special stains with reference to: Von Kossa SOP SS-25 Wade Fite SOP SS-26 Warthin and Starry SOP SS-27 Ziehl Neelson SOP SS-29 following manufacturer s instructions for Ventura BenchMark Ultra automated stainer SOP ICC-22 AE1/3 34BE12 BCL-2 BCL-6 Ber-EP4 CA19.9 Ca125 Calretinin Cytokeratin 7 and 8 CAM 5.2 T-cell marker T-cell marker Immature B cells and T cells CD3 CD5 CD10 Assessment Manager: EWS Page 4 of 8

5 tissue sections Immunohistochemical examinations for the purpose of clinical diagnosis Granulocytic cells following manufacturer s instructions for Ventura BenchMark Ultra automated stainer SOP ICC-22 CD15 B cells Dendritic cell marker Dendritic cell marker, expresses B cells of germinal centres Anaplastic large cell lymphomas and Reed Stemberg cells Endothelial cells CD20 CD21 CD23 CD30 CD34 Cells of haematopoietic lineage CD45 Human CD56 molecule on NK cells CD56 Macrophages and myeloid cells Transmembrane tyrosine kinase receptor Late stage B cell differentiation Carcinoembryonic antigen Carcinoembryonic antigen Secretory granules of endocrine cells Cytokeratin 5 intermediate filament protein Cytokeratin 7 intermediate filament protein CD68 CD117 CD138 CEA (mono) CEA (poly) Chromogranin A CK5 CK7 Assessment Manager: EWS Page 5 of 8

6 tissue sections Immunohistochemical examinations for the purpose of clinical diagnosis Cytokeratin 7 intermediate filament protein Cytokeratin 20 intermediate filament protein Cyclin D molecule Intermediate filament protein in muscle Adhesion protein Epithelial membrane antigen Oestrogen receptor following manufacturer s instructions for Ventura BenchMark Ultra automated stainer SOP ICC-22 CK7 CK20 Cyclin D1 Desmin E-cadherin EMA ER Endothelial cells Factor 8 Erythroid precursors and erythrocytes Trophoblasts Her 2 antigen Immature melanosomes Peptide hormone Kappa light chains in plasma cells and related lymphoid cells Proliferation marker Lambda light chains of myelomas, plasmacytomas and certain non- Hodgkin s lymphomas Melanocytes and melanosomes Glycophorin A bhcg Her2/Neu HMB45 Inhibin Kappa Ki-67 Lambda MART-1/Melan A Assessment Manager: EWS Page 6 of 8

7 tissue sections Immunohistochemical examinations for the purpose of clinical diagnosis Mis match repair Granulocytes and monocytes Neuroendocrine marker Placental alkaline phosphatase Basal cell marker Progesterone receptor Prostate specific antigen in prostatic epithelium Prostatic acid phosphatase Low molecular weight protein Microfilament cytoskeleton proteins in smooth muscle Presynaptic and peripheral nervous system Endothelium, squamous cell carcinoma, mesangial cells Thyroid follicular epithelium and colloid, follicular neoplasms of the thyroid, papillary and hurtle cell carcinomas of the thyroid Thyroid transcription factor 1 Mesenchymal cells following manufacturer s instructions for Ventura BenchMark Ultra automated stainer SOP ICC-22 MMR panel MPO NSE PLAP P63 PR PSA PAP S100 SMA SYNAP Thrombomodulin (CD141) Thyroglobulin TTF1 Vimentin Assessment Manager: EWS Page 7 of 8

8 Body tissues and fluids, various sites as below Bronchial washings Cerebrospinal fluid Oesophageal and gastric washings Sputum Skin scrapings Clots and tissue fragments Fine needle aspiration of body fluid Cytological examinations for the purpose of clinical diagnosis Cytopathology (non-gynae) Examination of cellular material in order to identify or exclude morphological and cytological abnormalities Reporting of Histopathology and Cytopathology based on equipment manuals as relevant Using: Thermo Shandon Cytospin4 SOP N005 Leica Autostainer XL SOP N025 Leica CV5030 cover slipper SOP ML- 03 Papanicolaou staining SOP N025 SOP N007 SOP N008 SOP N011 SOP N012 SOP N013 SOP N020 SOP N014, N015 SOP OFF/07, OFF/08 END Assessment Manager: EWS Page 8 of 8