NEW Kinetex F5. HPLC/UHPLC Core-Shell Columns

Transcription

1 Core-Shell Technology NEW Kinetex Whoa! I can even separate structural isomers! TMS TMS HPLC/UHPLC Core-Shell Columns Reduce Method Development Time by Days Greater Reproducibility than other PPs Glorious Interaction Mechanisms Valuable LC Separation Modes

2 How I Work With the astonishing combination of core-shell performance and interaction mechanisms, Kinetex columns will effortlessly drive your orthogonal HPLC/UHPLC development! A Hydrophobic Carbon skeleton of linker and ring encourage neutral/hydrophobic retention B Aromatic In non-acetonitrile mobile phases, π- π electrons of the carbon ring interact with analyte π- π electrons and result in positive retention increase C A TMS D B TMS E C D E Electrostatic High electronegativity of the fluorine groups create dipole moments, aiding in polar compound retention. Induced dipole moments can also aid neutral compound retention. Steric/Planar Shape selectivity allows for isomeric separations that are otherwise impossible on traditional alkyl phases. Hydrogen Bonding Extremely effective retention mechanism caused as polar functional groups of analyte interact with the electron greedy fluorine. Phenomenex, Inc. All rights reserved. Phenomenex l WEB:

3 Core-Shell Technology Why I m Better While older pentafluorophenyl phases (PP, PPP,, etc.) are based on existing bonding techniques and technologies that promote irreproducibility, the Kinetex was meticulously designed by Phenomenex R&D and its customers, to provide consistently accurate and high performance results. Learn how the new Kinetex will get you the results you deserve time and time again! Core-Shell Advantage...p. Method Development Versatility...p. Unmatched Reproducibility... pp. - % Aqueous Stability...p. 8 Isomeric Separations...p. 9 Vitamin D Epimers... p. Multi Component Screening... p. Methanol vs. Acetonitrile... p. Trace Impurity Detection... p. UHPLC Consistency and Performance...pp. - Kinetex Column Selection...pp. - Ordering Information...pp. 8-9 And I m guaranteed! If you are not completely satisfied with Kinetex core-shell columns, send in your comparative data to a similar product with the Kinetex column within days for a ULL REUND.

4 Why Wait? You Can Get Improvements Today! The band broadening (wide peaks) and lengthy retention times of traditional fully porous products can be limiting your results. Now you can use the incredible efficiency levels of Kinetex Core- Shell Technology to achieve shorter run times, higher levels of sensitivity, and overall better HPLC/UHPLC results. Core-Shell vs. ully Porous Efficiency Levels (plates/m) Kinetex Core-Shell. µm Kinetex Core-Shell. µm ully Porous. µm ully Porous µm ully Porous µm,,,,,,, Core-Shell Performance Gains Kinetex Core-Shell ully Porous Get faster, better results with me! APP ID 8 8 min Phenomenex l WEB:

5 Core-Shell Technology Method Development Versatility Combine core-shell performance, multiple retention mechanisms and the Kinetex column s ability to be run in a variety of separation modes and you now have an impeccable method development tool at your disposal. Reversed Phase Separate compound mixtures with moderately polar mobile phase and the polar/non-polar selectivity of Kinetex % Aqueous ocus on polar compound retention with completely polar mobile phase D-LC Utilize the Kinetex orthogonal selectivity in series with a C8 for identification work SC Supercritical carbon dioxide (CO ) and modifier (usually alcohols) mobile phase with Kinetex for achiral separation TMS TMS HILIC Polar selectivity of Kinetex is used in combination with high organic mobile phase to concentrate on polar compound retention

6 Dependability Batch-to-Batch, Column-to-Column Conventional fully porous and core-shell PP/ columns fail to reach the level of repeatability that you deserve. Inconsistencies in their base silica have led to data inaccuracies that waste your time and money. Kinetex columns were specifically designed to avoid these past problems and provide you with the highest degree of reproducibility. Kinetex. µm min min BATCH BATCH BATCH min APP ID 8 APP ID 8 APP ID 8 Supelco Ascentis Express. µm min min BATCH A BATCH B BATCH C min APP ID 8 APP ID 8 APP ID 8 Conditions for all columns: Column: Kinetex. µm Discovery µm HS Ascentis Express. µm Hypersil GOLD µm PP Accucore. µm PP Dimensions: x. mm Mobile Phase: A:. % ormic acid in Water B:. % ormic acid in Acetonitrile Gradient: -9 % B over minutes low Rate:.8 ml/min Temperature: Ambient Detection: nm Sample:. Uracil. Pindolol. Chlorpheniramine. Nortriptyline. -Methyl--Nitrobenzoic acid. -Methyl Salicyl Aldehyde. Hexaphenone What happened to peaks and? Accucore is a trademark and Hypersil GOLD is registered trademark of Thermo Hypersil-Keystone. Ascentis, Discovery and Supelco are registered trademarks of Sigma-Aldrich Co. LLC. Phenomenex is not affiliated with any of the above companies. Comparative separations may not be representative of all applications. Phenomenex l WEB:

7 Core-Shell Technology Supelco Discovery µm HS Thermo Hypersil GOLD µm PP min min min BATCH A BATCH B BATCH C APP ID 8 APP ID 8 APP ID 8, BATCH A min, BATCH B min BATCH C, min APP ID 8 APP ID 8 APP ID 8 Thermo Accucore. µm PP min min min Adsorption of Peaks and! BATCH A BATCH B BATCH C APP ID 8 APP ID 8 APP ID 8 Conditions for all columns: Column: Kinetex. µm Discovery µm HS Ascentis Express. µm Hypersil GOLD µm PP Accucore. µm PP Dimensions: x. mm Mobile Phase: A:. % ormic acid in Water B:. % ormic acid in Acetonitrile Gradient: -9 % B over minutes low Rate:.8 ml/min Temperature: Ambient Detection: nm Sample:. Uracil. Pindolol. Chlorpheniramine. Nortriptyline. -Methyl--Nitrobenzoic acid. -Methyl Salicyl Aldehyde. Hexaphenone Accucore is a trademark and Hypersil GOLD is registered trademark of Thermo Hypersil-Keystone. Ascentis, Discovery and Supelco are registered trademarks of Sigma-Aldrich Co. LLC. Phenomenex is not affiliated with any of the above companies. Comparative separations may not be representative of all applications. Retention Time Shifting Are you okay with peak adsorptions and retention time shifts?

8 Extra Polar Retention and % Aqueous Stability Unlike traditional alkyl stationary phases, the polar functional group of the Kinetex makes it stable in % aqueous mobile phase conditions. This can be especially beneficial for methods that require the retention and resolution of problematic polar compounds that typically have poor retention under reversed phase conditions. Organic Acids DAY APP ID 8 min umaric acid O Column: Kinetex. µm Dimensions: x. mm Part No.: D--E Mobile Phase: mm Sodium phosphate ph. low Rate:. ml/min Temperature: Ambient Detection: nm Sample:. Tartaric Acid. Malic Acid. Maleic Acid. umaric Acid. Succinic Acid DAY HO O OH APP ID 8 min O Maleic acid OH O OH INCREDIBLE! Even the isomers Maleic acid and umaric acid are separated! DAY APP ID 8 min Each successive day, column was equilibrated, run and stored in the % aqueous conditions mentioned above. 8 Phenomenex l WEB:

9 Core-Shell Technology Novel Selectivity or Isomeric Separations While a C8 can differentiate between the small addition of a single methyl group between two similar compounds, it cannot separate compounds with only structural differences, like positional isomers. This is where the electrostatic and planar interactions of the Kinetex give focused attention to the resolution you need. 8 8 Methoxybenzene Isomers 8 min,, KINETEX KINETEX C8 + Hydrophobic + Aromatic + Hydrogen Bonding + Electrostatic + Planar/Steric + Hydrophobic APP ID 8 Conditions for all columns: Column: Kinetex. µm Kinetex. µm C8 Kinetex. µm Biphenyl Dimensions: x. mm Mobile Phase: A:.% TA in Water B: Acetonitrile Isocratic: A/B (:) low Rate: ml/min Temperature: Ambient Detection: nm Sample:.,,-Trimethoxybenzene.,-Dimethoxybenzene.,,-Trimethoxybenzene.,-Dimethoxybenzene. Methoxybenzene.,-Dimethoxybenzene APP ID 88 8 min 8, KINETEX BIPHENYL + Hydrophobic + Aromatic + Some Electrostatic + Some Planar/Steric APP ID 89 8 min 9

10 Clinical Research Vitamin D Epimers Even tandem mass spectrometry (LC/MS/MS) analysis has need to utilize the wonderful cross functionality of Kinetex. With the same fragment ions coming from the Vitamin D epimers, reproducible chromatographic separation is a must. The unique combination of polar/non-polar selectivity of Kinetex easily performs this necessary separation in a highly sensitive and short analysis window. Kinetex. µm.e.e.e.e.e.e 9.e 8.e.e.e.e.e.e.e.e Intensity, cps.e.e.e.e.e.e 9.e 8.e.e.e.e.e.e.e.e Intensity, cps.e.e.e.e.e.e 9.e 8.e.e.e.e.e.e.e.e Intensity, cps min Advanced Material Technology HALO. µm PP -OH VITAMIN D -OH VITAMIN D -EPI--OH VITAMIN D -OH VITAMIN D min Waters XSelect HSS. µm PP -OH VITAMIN D -OH VITAMIN D -EPI--OH VITAMIN D Baseline resolution! -EPI--OH VITAMIN D -OH VITAMIN D min APP ID 8 APP ID 8t APP ID 8 Conditions for all columns: Column: Kinetex. µm HALO. µm PP XSelect HSS. µm PP Dimensions: x. mm Mobile Phase: A:.% ormic acid in Water B:.% ormic acid in Methanol Isocratic: A/B (:8) low Rate:. ml/min Temperature: Ambient Detection: MS/MS (AB SCIEX API ) Sample:. -OH Vitamin D. -OH Vitamin D. -epi--oh Vitamin D Vitamin D epimers are not fully separated HALO is a registered trademark of Advanced Materials Technology, Inc. Waters and XSelect are registered trademarks of Waters Corporation. Phenomenex is not affiliated with any of the above companies. Comparative separations may not be representative of all applications. Phenomenex l WEB:

11 ood Testing Core-Shell Technology Multi Component Analysis The versatility of Kinetex core-shell columns matches marvelously with ingredient quantification and contaminant identification. Acidic food additives may tail and lack retention on a C8 column, but the Kinetex offers superb polar retention and performance. Meanwhile, large multi component contaminant screens and their mixtures of acids, bases, neutrals and structurally similar compounds, can easily utilize the combination of polar, non-polar and geometric interaction mechanisms of the Kinetex to get excellent separation and sensitivity. 8 ood Additives Intensity, cps Antibiotics Screen.e.e.e.e.e.e.e.e.e.e.e.e. min 8 9 min APP ID 8 APP ID 8 Column: Kinetex. µm Dimensions: x. mm Part No.: --AN Mobile Phase: A:.% Phosphoric acid in Water B: Acetonitrile Gradient: - % A in min. Hold for min. low Rate:. ml/min Temperature: C Detection: nm Sample:. Saccharin. p-hydroxybenzoic acid. Sorbic acid. Dehydroacetic acid. Methyl paraben ind more Kinetex applications at com/kinetexapps Column: Kinetex. µm Dimensions: x. mm Part No.: B--AN Mobile Phase: A:.% ormic acid in Water B:.% ormic acid in Methanol Gradient: Time (min) low Rate:. ml/min Temperature: C % B Detection: MS/MS (AB SCIEX API ) Sample:. Amoxicillin. Sulfamethazine. Cefalexin. Sulfamethoxazole. Cefazolin. Sulfapyridine. Cefoperazone. Suflaquinoxaline. Cefapirin. Sulfathiazole. Cloxacillin 8. Neospiramycin. Dicloxacillin 9. Doxycycline 8. Ciprofloxacin. Tiamulin 9. Difloxacin. Valnemulin. Marbofloxacin. Rifaximin. Sulfadiazine. Lincomycin. Sulfamerazine. Nafcillin

12 Methanol vs. Acetonitrile A Phenyl Story While mobile phase modifiers can help adjust retention, with the use of the Kinetex, the major mobile phase constituent can also be used to manipulate elution order and retention properties. Acetonitrile can be used to disrupt pi-pi interactions between compounds and phenyl phases, while switching to the weaker solvent Methanol will encourage aromatic interactions. 8 Methoxybenzene Isomers MOBILE PHASE: A:.% TA IN WATER B: ACETONITRILE APP ID 8 ACETONITRILE Conditions for all columns: Column: Kinetex. µm Dimensions: x. mm Part No.: --E Mobile Phase: as noted Isocratic: A/B (:) low Rate: ml/min Temperature: Ambient Detection: nm Sample:.,,-Trimethoxybenzene.,-Dimethoxybenzene.,,-Trimethoxybenzene min MOBILE PHASE: A:.% TA IN WATER B: METHANOL METHANOL APP ID 8-8 min Impact selectivity and retention with solvent adjustment! Phenomenex l WEB:

13 Trace Pharmaceutical Core-Shell Technology Impurity Detection Trace impurities of active pharmaceutical ingredients are incredibly important to identify and quantify. With the rapid performance value of core-shell technology combined with the versatility of a pentafluorophenyl, the Kinetex is the precise alternative to other reversed phase columns that you need. Easily utilize the Kinetex to get greater sensitivity, better resolution and all in shorter analysis times. Kinetex. µm Waters ACQUITY CSH. µm luoro-phenyl Resolution of Impurities APP ID 88, Impurities Are Not Resolved APP ID min 8 9 min Conditions for all columns: Column: Kinetex. µm ACQUITY CSH. µm luoro-phenyl Dimensions: x. mm Mobile Phase: A: mm Potassium phosphate ph. B: Methanol Gradient: Time (min). % B 9 low Rate:. ml/min Temperature: Ambient Detection: nm Sample:. Impurity. Impurity. Proprietary Active Pharmaceutical Ingredient Waters and ACQUITY are registered trademarks and CSH is a trademark of Waters Corporation. Phenomenex is not affiliated with Waters Corporation. Comparative separations may not be representative of all applications.

14 Incredible UHPLC Reproducibility Highly sensitive UHPLC separations are dependent upon consistent quantitation and consistent results. With the reliability of the Kinetex, you no longer have to settle for the inconsistent results of PP products that currently exist on the market. Our highest standards of quality will ensure that you are fully satisfied with each and every Kinetex column! Trusted Consistency BATCH APP ID 8 min BATCH Column: Kinetex. µm Dimensions: x. mm Mobile Phase: A:. % ormic in Water B:. % ormic in Acetonitrile Gradient: -9 % B over min. low Rate:.8 ml/min Temperature: Ambient Detection: nm Sample:. Uracil. Pindolol. Chlorpheniramine. Nortriptyline. -Methyl--Nitrobenzoic acid. -Methyl Salicyl Aldehyde. Hexaphenone APP ID 8 BATCH min Quality APP ID 8 min Phenomenex l WEB:

15 A Better PP Core-Shell Technology Selectivity for UHPLC Kinetex. μm core-shell technology produces increased efficiencies over traditional sub- μm columns on the market, yielding remarkable chromatographic resolution, higher peak capacities, and greater sensitivity. Add these performance gains alongside the novel selectivity and excellent reproducibility of the Kinetex and you now have an incredible UHPLC solution at your fingertips. AU AU AU Pharmaceutical Mix min min Adsorption of Peaks and! KINETEX. µm WATERS ACQUITY CSH. µm LUORO-PHENYL WATERS ACQUITY HSS. µm PP APP ID 8 APP ID 8 APP ID 8 Conditions for all columns: Column: Kinetex. µm ACQUITY CSH. µm luoro-phenyl ACQUITY HSS.8 µm PP Dimensions: x. mm Mobile Phase: A: mm Ammonium acetate ph. B: Acetonitrile Gradient: Time (min) low Rate:. ml/min Temperature: Ambient Detection: nm Sample:. Pindolol. Propanolol. Indoprofen. Naproxen. Warfarin. Terfenidine % B 9 9 MEOW!! Kinetex is amazing! min Waters and ACQUITY are registered trademarks, and CSH is a trademark of Waters Corporation. Phenomenex is not affiliated with Waters Corporation. Comparative separations may not be representative of all applications. Terrellasaurus first discovered in Torrance, CA.

16 Get Method Development lexibility or Your Small Molecule Analysis Recommended Selectivities If You re Working With: Acids Bases Neutrals Aromatics Kinetex C8 Kinetex Kinetex Phenyl-Hexyl Kinetex EVO C8 Kinetex XB-C8 Kinetex Biphenyl Kinetex C8 Kinetex C8 Kinetex Biphenyl Kinetex Biphenyl Kinetex Phenyl-Hexyl Kinetex Acids, Bases, and Neutrals Highly Polar Compounds High ph Isomers Kinetex C8 Kinetex Biphenyl Kinetex EVO C8 Kinetex Kinetex EVO C8 Kinetex Kinetex Biphenyl Kinetex HILIC Kinetex EVO C8 Kinetex Upgrading Your ully Porous Methods: ully Porous µm or µm ully Porous sub- µm ully Porous Preparative LC Kinetex µm Drop-in for easy performance improvements with no backpressure increase Kinetex. µm Dramatically improve results with efficiency/peak capacity gains Kinetex. µm Get similar efficiencies at lower backpressure allowing for greater productivity gains Kinetex. µm Up to % greater efficiencies resulting in drop-in improvements Kinetex. µm Incredible efficiency gains on high end UHPLC systems Kinetex µm Drop-in for easy performance improvement with no backpressure increase Phenomenex l WEB:

17 Choosing The Best Core-Shell Platform or You is Easy! Core-Shell Technology or Small Molecules UHPLC HPLC PREP LC µm. µm. µm. µm. µm Phase Best Use ph Stability Available Particle Size(s) EVO C8 C8 XB-C8 C8 Biphenyl Phenyl-Hexyl HILIC Highly reproducible pentafluorophenyl propyl phase that offers a unique combination of polar, hydrophobic, aromatic, and shape selectivity Robust reversed phase methods even in alkaline conditions with improved peak shape for polar basic compounds All purpose phase that offers the hydrophobic retention and methylene selectivity chromatographers expect from a C8 column C8 phase with protective butyl side chains for improved peak shape for basic compounds under neutral and acidic conditions USP L phase that provides less hydrophobic and methylene selectivity than a C8 % aqueous stable and allows for excellent reversed phase retention and enhanced polar and aromatic selectivity Reversed phase chemistry that allows for greater retention and separation of aromatic hydrocarbons Unbonded silica phase for HILIC conditions to provide selectivity for polar compounds *. µm. µm - µm * µm. µm. µm. µm * µm. µm. µm * µm. µm. µm * µm. µm. µm * µm. µm. µm. -. µm. µm. µm Phenomenex Application Specific Core-Shell Products Material Phase Best Use ph Stability Available Particle Size(s) or Peptides (, Da) Aeris PEPTIDE XB-C8 Excellent hydrophobicity and methylene selectivity for peptide and peptide mapping separations µm. µm. µm. µm or Proteins (>, Da) Aeris WIDEPORE XB-C8 XB-C8 C Maximum hydrophobicity and high temp stability for hydrophilic and PEGylated proteins Medium hydrophobicity and high temp stability for moderately hydrophobic proteins and glycosylated proteins Lowest hydrophobicity for very large or very hydrophobic proteins µm µm µm or Synthetic Oligonucleotides (DNA/RNA) Clarity Rapid, high efficiency reversed phase LC/MS analysis for QC C8 Oligo-MS and characterization *ph stability under gradient conditions. ph stability is.- under isocratic conditions *. µm. µm

18 Ordering Information SecurityGuard μm Minibore Columns (mm) ULTRA Cartridges Phases x. x. x. x. /pk 8 EVO C8 A--AN B--AN D--AN --AN AJ-998 Biphenyl A--AN B--AN D--AN AJ-99 XB-C8 A--AN B--AN D--AN AJ-88 C8 A--AN B--AN D--AN --AN AJ-88 C8 B-8-AN D-8-AN AJ-88 Phenyl-Hexyl B--AN D--AN AJ-888 for. mm ID SecurityGuard μm MidBore Columns (mm) ULTRA Cartridges Phases x. x. x. /pk EVO C8 B--Y D--Y --Y AJ-99 Biphenyl B--Y D--Y --Y AJ-98 XB-C8 B--Y D--Y --Y AJ-8 C8 B--Y D--Y --Y AJ-8 C8 B-8-Y D-8-Y AJ-8 Phenyl-Hexyl B--Y D--Y AJ-88 for. mm ID SecurityGuard μm Analytical Columns (mm) ULTRA Cartridges Phases x. x. x. x. /pk EVO C8 B--E D--E --E G--E AJ-99 Biphenyl B--E D--E --E G--E AJ-9 XB-C8 B--E D--E --E G--E AJ-88 C8 B--E D--E --E G--E AJ-88 C8 B-8-E D-8-E -8-E G-8-E AJ-8 Phenyl-Hexyl B--E D--E --E G--E AJ-8 for. mm ID SecurityGuard μm Semi-Preparative Columns (mm) SemiPrep Cartridges*** Phases x x x /pk C8 --N G--N AJ-98 Biphenyl --N G--N AJ-98 for mm ID SecurityGuard μm Axia Packed Preparative Columns (mm) PREP Cartridges* Phases x. x. x. x. x. /ea EVO C8 B--P-AX D--P-AX --P-AX G--P-AX AJ-9 /ea Biphenyl B--P-AX D--P-AX --P-AX G--P-AX AJ-9 XB-C8 B--P-AX D--P-AX --P-AX G--P-AX AJ-9 C8 B--P-AX D--P-AX --P-AX G--P-AX AJ-9 C8 B-8-P-AX D-8-P-AX -8-P-AX G-8-P-AX AJ-9 Phenyl-Hexyl B--P-AX D--P-AX --P-AX G--P-AX AJ-9 HILIC D--P-AX --P-AX G--P-AX AJ-9 for. mm ID SecurityGuard μm Axia Packed Preparative Columns (mm) PREP Cartridges** Phases x x x x x /ea EVO C8 D--U-AX --U-AX G--U-AX AJ-9 /ea Biphenyl --U-AX AJ-9 XB-C8 B--U-AX D--U-AX --U-AX G--U-AX AJ-9 C8 B--U-AX D--U-AX --U-AX G--U-AX AJ-9 C8 B-8-U-AX D-8-U-AX -8-U-AX G-8-U-AX AJ-9 Phenyl-Hexyl B--U-AX D--U-AX --U-AX G--U-AX AJ-9 for mm ID Phenomenex l WEB: Protect Your LC Investment! novum simplified liquid extraction Remove interferences such as proteins, phospholipids, salts, and more in a quick and simple procedure Convenient and inexpensive way to remove particulates from HPLC samples Protect your Kinetex LC columns from contaminants and extend their lifetime. If you are not completely satisfied with Kinetex core-shell columns, send in your comparative data to a similar product with the Kinetex column within days for a ULL REUND. SecurityGuard ULTRA Cartridges require holder, Part No.: AJ-9 * PREP SecurityGuard Cartridges require holder, Part No.: AJ-8 ** PREP SecurityGuard Cartridges require holder, Part No.: AJ-8 *** SemiPrep SecurityGuard Cartridges require holder, Part No.: AJ-98

19 Ordering Information Core-Shell Technology SecurityGuard. μm Minibore Columns (mm) ULTRA Cartridges Phases x. x. x. x. x. /pk NEW NEW NEW B--AN D--AN --AN AJ-9 Biphenyl A--AN B--AN D--AN --AN AJ-99 XB-C8 A-9-AN B-9-AN C-9-AN D-9-AN -9-AN AJ-88 C8 A--AN B--AN C--AN D--AN --AN AJ-88 C8 A-9-AN B-9-AN C-9-AN D-9-AN -9-AN AJ-88 HILIC A--AN B--AN C--AN D--AN --AN AJ-88 Phenyl-Hexyl A-9-AN B-9-AN C-9-AN D-9-AN -9-AN AJ-888 for. mm ID SecurityGuard. μm MidBore Columns (mm) ULTRA Cartridges Phases x. x. x. x. x. /pk B--Y D--Y --Y AJ-9 Biphenyl B--Y D--Y --Y AJ-98 XB-C8 A-9-Y B-9-Y C-9-Y D-9-Y -9-Y AJ-8 C8 A--Y B--Y C--Y D--Y --Y AJ-8 C8 A-9-Y B-9-Y C-9-Y D-9-Y -9-Y AJ-8 HILIC A--Y --Y AJ-89 Phenyl-Hexyl B-9-Y D-9-Y -9-Y AJ-88 for. mm ID SecurityGuard. μm Analytical Columns (mm) ULTRA Cartridges Phases x. x. x. x. x. /pk B--E D--E --E AJ-9 Biphenyl B--E D--E --E AJ-9 XB-C8 B-9-E C-9-E D-9-E -9-E AJ-88 C8 A--E B--E C--E D--E --E AJ-88 C8 B-9-E C-9-E D-9-E -9-E AJ-8 HILIC B--E C--E D--E --E AJ-8 Phenyl-Hexyl B-9-E C-9-E D-9-E -9-E AJ-8 for. mm ID SecurityGuard. μm Minibore Columns (mm) ULTRA Cartridges Phases x. x. x. x. /pk NEW B--AN D--AN --AN AJ-9 Biphenyl B-8-AN D-8-AN -8-AN AJ-99 XB-C8 A-98-AN B-98-AN D-98-AN -98-AN AJ-88 C8 A--AN B--AN D--AN --AN AJ-88 C8 A-99-AN B-99-AN D-99-AN -99-AN AJ-88 HILIC A--AN B--AN D--AN AJ-88 Phenyl-Hexyl B--AN D--AN --AN AJ-888 for. mm ID SecurityGuard. μm MidBore Columns (mm) ULTRA Cartridges Phases x. x. x. /pk XB-C8 A-98-Y B-98-Y D-98-Y AJ-8 C8 B--Y D--Y AJ-8 C8 A-99-Y B-99-Y D-99-Y AJ-8 HILIC B--Y AJ-89 for. mm ID. μm Minibore Columns (mm) Phases x. x. C8 A--AN B--AN SecurityGuard ULTRA Cartridges require holder, Part No.: AJ-9 Terms and Conditions Subject to Phenomenex Standard Terms & Conditions, which may be viewed at Trademarks Phenomenex, Clarity, and Kinetex are registered trademarks of Phenomenex. Axia, Aeris, Oligo-MS, MidBore, Novum, Phenex, and SecurityGuard are trademarks of Phenomenex. Waters, ACQUITY, and XSelect are registered trademarks, and CSH is a trademark of Waters Corporation. Agilent and Poroshell are registered trademarks of Agilent Technologies, Inc. Ascentis and Supelco are registered trademarks of Sigma-Aldrich Co. LLC. ACE is a registered trademark and UltraCore is a trademark of Advanced Chromatography Technologies Limited. Accucore is a trademark and Hypersil GOLD is registered trademark of Thermo Hypersil-Keystone. API is a trademark of AB SCIEX Pte. Ltd. Disclaimer Phenomenex is not affiliated with Advanced Materials Technology, Inc., Agilent Technologies, Sigma-Aldrich Co., Thermo Hypersil-Keystone or Waters Corporation. Comparative separations may not be representative of all applications. SecurityGuard is patented by Phenomenex. U.S. Patent No.,,. CAUTION: this patent only applies to the analytical-sized guard cartridge holder, and does not apply to SemiPrep, PREP or ULTRA holders, or to any cartridges. Axia column and packing technology is patented by Phenomenex. U.S. Patent No.,, 8 Kinetex EVO is patented by Phenomenex. U.S. Patent Nos.,, and 8,8,8 and foreign counterparts. Phenomenex, Inc. All rights reserved. 9

20 NEW Kinetex HPLC/UHPLC Core-Shell Columns Reduce Method Development Time by Days Greater Reproducibility than other PPs Core-Shell Technology Glorious Interaction Mechanisms Valuable LC Separation Modes TMS TMS Australia t: + ()-98- f: + ()-98- auinfo@phenomenex.com Austria t: + ()-9- f: + ()-9- anfrage@phenomenex.com Belgium t: + () (rench) t: + () 8 (Dutch) f: + ()-89 beinfo@phenomenex.com Canada t: + (8) -8 f: + () 8-8 info@phenomenex.com China t: +8 () 8-8 f: +8 () 89-8 chinainfo@phenomenex.com Denmark t: f: + 8 nordicinfo@phenomenex.com inland t: +8 ()9 89 f: + 8 nordicinfo@phenomenex.com rance t: + () 9 f: + () 9 franceinfo@phenomenex.com Germany t: +9 ()-88- f: +9 ()-88- anfrage@phenomenex.com India t: +9 ()- f: +9 ()- indiainfo@phenomenex.com Ireland t: + () f: + -9 eireinfo@phenomenex.com Luxembourg t: + ()-8 f: + ()-89 nlinfo@phenomenex.com Mexico t: f: tecnicomx@phenomenex.com The Netherlands t: + ()-8 f: + ()-89 nlinfo@phenomenex.com New Zealand t: + ()9-89 f: + ()9-89 nzinfo@phenomenex.com Norway t: + 8 f: + 8 nordicinfo@phenomenex.com Puerto Rico t: + (8) -HPLC f: + () 8-8 info@phenomenex.com Spain t: f: espinfo@phenomenex.com Sweden t: + ()8 9 f: + 8 nordicinfo@phenomenex.com United Kingdom t: + ()- f: + ()-9 ukinfo@phenomenex.com USA t: + () - f: + () 8-8 info@phenomenex.com All other countries Corporate Office USA t: + () - f: + () 8-8 info@phenomenex.com Italy t: +9 f: +9 italiainfo@phenomenex.com BR8_W Phenomenex products are available worldwide. or the distributor in your country, contact Phenomenex USA, International Department at international@phenomenex.com