World Renowned HPLC Media Reinvented for UHPLC!

Transcription

1 OMEGA World Renowned HPLC Media Reinvented for UHPLC! New. µm Staggering UHPLC Efficiencies and Performance Extreme Ruggedness and Mechanical Strength Perfect Complement to Kinetex Core-Shell Technology µm µm. µm Two Superb New Polar Selectivities for UHPLC and HPLC Polar C8 (. µm, µm, µm) PS C8 (. µm, µm, µm)

2 Enhanced with Years of Technology, Innovation, and Experience One of the world s leading HPLC brands, now enhanced for incredible UHPLC performance!. µm UHPLC columns culminate years of technological prowess, advancements, and innovation from Phenomenex! With astounding efficiency levels, highly versatile selectivities, and trusted accuracy, columns will take your UHPLC experience to a new level. Luna Omega UHPLC columns will boost your UHPLC instrumentation!

3 Why Luna Omega Should be in Your Lab? Omega Cutting-Edge UHPLC... p. Astounding Performance... p. Separation Muscle... p. Inert Foundation... p. Excellent Reproducibility... pp. 8-9 C8 C8 Selectivity... p. Utilizing Hydrophobicity... p. Polar C8 Polar C8 Selectivity... p. Enhanced Polar Retention... p. % Aqueous Stability... p. Polar and Non-polar Selectivity... p. Upgrading C8 Methods... p. Improving Existing Polar Methods... p. Polar Case Study: Catecholamines and PMETs... p. 8 Polar Case Study: ETG/ETS... p. 9 PS C8 PS C8 Selectivity... p. Enhanced Retention of Acids... p. Better Peak Shape for Bases... p. Excellent Loadability... p. Greater Method Flexibility... p. Improving Existing Basic Compound Methods... p. Reinvented Use Luna and Phases Together... p. UHPLC to HPLC to PREP Scalability... p. C8 and Polar C8 - Complementary Work Horses... pp. 8-9 Pairing With Core-Shell Technology... pp. - Column Lifetime... p. Sample Preparation... p. UHPLC Column Protection Guard Cartridge System... p. HPLC Column Protection Guard Cartridge System... p. Ordering Information... pp. -9 Phenomenex, Inc. All rights reserved.

4 Cutting-Edge. μm Silica Particle Luna is one of the most recognized HPLC brands on the market, delivering high efficiency, ruggedness, reproducibility and dependability for a wide range of analyses. The new. μm builds upon this legacy with an innovative yet rugged UHPLC silica particle architecture, designed and manufactured by Phenomenex based on more than years of applied knowledge, invention, and customer experience. Novel Design and Manufacturing Process Within the novel manufacturing process of. µm silica, we implement a proprietary processing technique to gain greater particle inertness, a stronger particle morphology, and more consistent porosity. Thermal Modified Pore Structure Consistent Porosity Most importantly, through our proprietary process, we eliminate micropores, further improving column efficiency, inertness, and reproducibility. Absence of Micropores C8 Polar C8 PS C8 TMS TMS Polar TMS TMS Polar + TMS TMS +

5 Astounding Performance The undeniably high efficiency levels found in each UHPLC column provide you with the potential of huge gains in method performance. While traditional silica and hybrid fully porous particles claim high performance, when compared to. μm, they drastically fall short and prevent UHPLC scientists from reaching their UHPLC potential. UHPLC Efficiency Comparison Luna Omega. µm C8 Thermo Scientific Hypersil GOLD.9 µm C8 Agilent ZORBAX Eclipse.8 µm XDB-C8 Thermo Scientific Syncronis. µm C8 Waters ACQUITY HSS.8 µm C8 Waters ACQUITY BEH. µm C8 AkzoNobel Kromasil.8 µm C8 ACE Excel µm C8 YMC -Triart.9 µm C8 Agilent ZORBAX.8 µm SB-C8,,,,,,, Efficiency (plates/meter) Dimension: x. mm Mobile Phase: Acetonitrile/Water (:) Flow Rate:. ml/min Temperature: Ambient Detection: nm System: ACQUITY UPLC

6 Separation Muscle Our industry leading bonding technologies in conjunction with high efficiency levels ensure excellent stationary phase coverage and improved separation power. Now, with. μm, you can turn difficult separations into resolution achievements. Luna Omega. µm C8.e.e.e.e.e.e.e 9.e 8.e.e.e.e.e.e.e.e Much Greater Resolution! min App ID 9 ACE Excel µm C8-AR.e.e.e.e.e.e.e 9.e 8.e.e.e.e.e.e.e.e. min App ID Agilent ZORBAX.8 µm XDB-C8.e.e.e.e.e.e.e.e.e.e.e.e.e.e.e. min App ID Columns:. µm C8 ZORBAX.8 µm XDB-C8 ACE Excel µm C8-AR Dimension: x. mm Mobile Phase: A:. % Formic Acid in Water B:. % Formic Acid in Methanol 9 Flow Rate:. ml/min Temperature: C Detection: MS/MS Sample:. Succinic acid. MMA. Glutaric acid. Methylsuccinate. Ethylmalonic acid. Hippuric acid. Homovanillic acid 8. Suberic acid

7 Inert Foundation UHPLC columns contain a unique silica modified using a proprietary, post-synthetic thermal treatment process to provide extraordinary mechanical strength and significantly greater inertness than traditional fully porous and hybrid materials. This greatly minimizes secondary interactions that negatively affect peak shape, allowing for greater method accuracy. Luna Omega. µm C Excellent Peak Shape App ID 8 min Waters ACQUITY BEH. µm C Poor Peak Shape App ID 8 min Thermo Scientific Hypersil GOLD.9 µm C Poor Peak Shape App ID 8 min Columns:. µm C8 ACQUITY BEH. µm C8 Hypersil GOLD.9 µm C8 Dimension: x. mm Mobile Phase: A:. % Formic Acid in Water B:. % Formic Acid in Acetonitrile Flow Rate:. ml/min Temperature: Ambient Detection: nm Sample:. Pindolol. Chlorpheniramine. Nortripytline. -Methyl--nitrobenzoic acid. -Methyl salicylaldehyde. Hexanophenone

8 Consistent Results C8 Batch-to-batch and column-to-column, Luna Omega media and columns are designed to be consistent and incredibly accurate tools for your analysis. Each batch and column are quality tested to ensure dependability and reproducibility. C8 Batch A Peak Capacity 8 App ID min C8 Batch B...8 Peak Capacity... 8 App ID min C8 Batch C Peak Capacity 8 App ID min Columns:. µm C8 Dimension: x. mm Part No.: B--AN Mobile Phase: A: Water B: Acetonitrile. 8 Flow Rate:. ml/min Temperature: Ambient Detection: nm Sample:. Estriol. Prednisolone. Hydrocortisone. Cortisone. Cortisone Acetate. -Hydroxycortisone. -Hydroxycortisone 8. Deoxycorticosterone 8

9 Consistent Results Polar C8 Just like Luna Omega C8, the Polar C8 is also stringently tested to make sure both batchto-batch and column-to-column quality stays at the highest level before it reaches your lab bench. Polar C8 Batch A Peak Capacity min App ID Polar C8 Batch B Peak Capacity QUALITY MANAGEMENT SYSTEM CERTIFIED BY DNV GL 9:8 min App ID Polar C8 Batch C min Peak Capacity App ID Columns:. µm Polar C8 Dimension: x. mm Part No.: B-8-AN Mobile Phase: A:. % Formic Acid in Water B:. % Formic Acid in Acetonitrile 9 Flow Rate:. ml/min Temperature: C Detection: nm Sample:. Uracil. Pindolol. Chlorpheniramine. Nortriptyline. -Methyl--nitrobenzoic acid. -Methyl salicylaldehyde. Hexanophenone 9

10 Selectivity Highlight C8 C8 Luna Omega C8 is an excellent first choice for chromatographers who are just starting method development or attempting to improve upon existing chromatographic results with other C8s. With its higher performance potential, excellent retention profile, and greater inertness, the C8 was designed to be the new all-purpose UHPLC solution for industries all over the world. Phase C8 Particle Size. µm Pore Size Å ph Range. - 8.* Surface Area m /g Carbon Load % Pressure Limit bar USP Listing L *ph stability under gradient conditions. ph stability is.- under isocratic conditions. TMS TMS Greater Retention and Better Results Higher efficiency levels in combination with excellent stationary phase coverage and greater particle inertness, translates to improved separation power for you. Now you can utilize the greater retention of Luna Omega C8 to tackle both easy and difficult separations.. µm C8 AU... min Waters ACQUITY BEH. µm C8 AU Peak Capacity 9 App ID 8 Peak Capacity App ID 8 Greater Peak Capacity and Retention Columns:. μm C8 ACQUITY BEH. μm C8 Dimension: x. mm Mobile Phase: A:. % Formic Acid in Water B:. % Formic Acid in Acetonitrile.. Flow Rate:. ml/min Temperature: Ambient Detection: nm Sample:. Acetaminophen. -Aminobenzoic Acid. -Hydroxybenzoic Acid. -Acetaminophenol. -Hydroxybenzoic Acid. Salicylicamide. Phenol 8. Benzoic Acid 9. Salicylic Acid... min

11 Small and Large Compound Mixtures Strong and focused hydrophobic retention, incredible efficiency and valuable inertness of Luna Omega C8 columns make them an excellent choice for small mixtures of compounds differing in hydrophobicity as well as large mixtures of compounds like impurity/degradation profiles and peptide maps. Phenols AU Columns:. µm C8 Dimension: x. mm Part No.: B--AN Mobile Phase: A:. % Formic Acid in Water B:. % Formic Acid in Acetonitrile. 9 Flow Rate:. ml/min Temperature: C Detection: nm Sample:. -Hydroxyphenol. Phenol. -Nitrophenol. -Methylphenol. -Methylphenol.,-Dimethylphenol. -Naphthol App ID min Synthetic Cannabinoids.e.e.e.e.e 9.e 8.e.e.e.e.e.e.e.e. 8 9 min Columns:. µm C8 Dimension: x. mm Part No.: B--AN Mobile Phase: A:. % Formic Acid in Water B:. % Formic Acid in Acetonitrile Flow Rate:. ml/min Temperature: C Detection: MS/MS (SCIEX API ) Sample:. JWH- Butanoic acid metabolite. JWH- -hydroxybutyl metabolite. JWH-8 Pentanoic acid metabolite. JWH- -hydroxybutyl metabolite. JWH-8 -hydroxypentyl metabolite. AM- -hydroxypentyl metabolite. JWH-8 -hydroxypentyl metabolite 8. AM-9 9. AM-. JWH-. JWH-8 App ID C8 OTC Drug - Pill Formulation Profile AU.... min Columns:. µm C8 Dimension: x. mm Part No.: B--AN Mobile Phase: A: mm Potassium Phosphate ph. B: Acetonitrile. 8 Flow Rate:. ml/min Temperature: Ambient Detection: nm Sample: OTC Drug Pill App ID Peptide Map Digested BSA.e9.e9 9.e8 8.e8.e8.e8.e8.e8.e8.e8.e8. App ID min Columns:. µm C8 Dimension: x. mm Part No.: D--AN Mobile Phase: A:. % Formic Acid in Water B:. % Formic Acid in Acetonitrile. Flow Rate:. ml/min Temperature: C Detection: MS/MS (SCIEX API ) Sample: Tryptic digest of BSA

12 Selectivity Highlight Polar C8 Polar C8 Luna Omega Polar C8 is a novel UHPLC stationary phase capable of providing a unique selectivity within a wide elution window and increased retention for both polar and non-polar analytes. The all-purpose C8 ligand provides hydrophobic interactions while a polar modified particle surface provides enhanced polar retention and also aqueous stability. These attributes make the Polar C8 an excellent choice for balanced retention of polar and hydrophobic compounds as well as to solely enhance retention of highly polar compounds. A C8, But Different Phase Polar C8 Particle Size. µm, µm, µm Pore Size Å ph Range. - 8.* Surface Area m /g Carbon Load 9 % Pressure Limit bar USP Listing L *ph stability under gradient conditions. ph stability is.- under isocratic conditions. Polar C8 is a uniquely modified C8-based chemistry that has been optimized to improve the performance of polar analyses. This new particle surface chemistry makes the Polar C8 applicable to all industries that utilize UHPLC for mixtures of polar and non-polar compounds. Polar TMS TMS Polar C8 silica surface Polar C8 silica surface TMS TMS TMS Polar Polar TMS

13 Greater Polar Retention With its polar modified surface, the Luna Omega Polar C8 offers UHPLC users enhanced separation power that can greatly improve resolution values for target compounds. Nicotine and Metabolites.8e.e.e.e.e.8e.e.e.e.e 8.e.e.e.e.e.e.e.8e.e.e.e.e 8.e.e.e.e + Catecholamines 8.e.e.e.e.e.e.e.e 8.e.e.e.e.e.e.e.e + Greater Retention Greater Retention. μm Polar C8 min min App ID 9 Waters CORTECS. µm C8 App ID 9. µm Polar C8 App ID 9 min Waters ACQUITY BEH. µm C8 App ID 9 min Columns:. µm Polar C8 CORTECS. µm C8 Dimension: x. mm Mobile Phase: A: mm Ammonium Formate with.% Formic Acid B: Acetonitrile with.% Formic Acid Columns:. µm Polar C8 ACQUITY BEH. µm C8 Dimension: x. mm Mobile Phase: A: Water with.% Formic Acid B: Acetonitrile with.% Formic Acid.. 9 Flow Rate:. ml/min Temperature: C Detection: MS/MS (SCIEX API ) Sample:. Nornicotine. -Hydroxycotinine. Nicotine. Cotinine. Anabasine 9 Flow Rate:. ml/min Temperature: C Detection: MS/MS (SCIEX API ) Sample:. Norepinephrine. Epinephrine. Normetanephrine. Dopamine. Metanephrine Polar C8

14 % Aqueous Stability Polar C8 % of Initial Retention Time No Stationary Phase Collapse Traditional C8 phases are known to collapse under % aqueous conditions, causing retention loss of compounds and a method development headaches. That is why an advanced proprietary bonding technology was used for the Luna Omega Polar C8 in order to ensure aqueous stability. The graph below displays the excellent stability of Polar C8 in % aqueous buffer conditions for over weeks. Aqueous Stability of Polar C8 % % 8% % % % % Adenine G--MP Thymine Columns:. µm Polar C8 Dimension: x. mm Part No.: B-8-AN Mobile Phase: mm Ammonium Formate with. % Formic Acid Flow Rate:. ml/min Temperature: C Detection: nm Sample:. Adenine. Guanosine--Monophosphate. Thymine App ID Duration of Exposure Day Day Nucleosides in % Aqueous Conditions Use % aqueous conditions for the greatest polar acid/base retention.. µm Polar C8 App ID 8 min Waters ACQUITY BEH. µm C8 App ID 8 min Columns:. µm Polar C8 ACQUITY BEH. µm C8 Dimension: x. mm Mobile Phase: mm Ammonium Formate ph. Flow Rate:. ml/min Temperature: C Detection: 8 nm Sample:. Thiourea. -Fluorocytosine. Guanosine--Monophosphate. Adenine. Thymine

15 Versatility Hydrophobic and Polar Interactions Combined retention of polars and non-polars by the Luna Omega Polar C8 gives you an incredible range of potential success when working with methods from just about any industry. Here we illustrate the usefulness of these interaction mechanisms with water soluble vitamins from drink and tablet, a multi-compound antibiotic screen, and a UHPLC/MS/MS separation of algal toxins. Water Soluble Vitamins Algal Toxins (EPA ).e.8e.e.e.e.e 8.e.e.e.e.e.e.e.e.e.e.e.e.e.e.e Columns:. µm Polar C8 Dimension: x. mm Part No.: B-8-AN Mobile Phase: A: mm Ammonium Formate with. % Formic Acid B: Acetonitrile with. % Formic Acid. 9 Flow Rate:. ml/min Temperature: C Detection: MS/MS (SCIEX API ) Sample:. Pyridoxamine. Thiamine. Nicotinic acid. Pyridoxine min Vitamin Tablet min. Pantothenic acid. Folic acid. Riboflavin Standards App ID 8 min Vitamin Enriched Drink App ID 8 App ID 9 Relative Abundance RT:. -. Uracil-d RT:.9 AA: 9 SN: Anatoxin-a RT:.8 AA: SN: RT:.9 AA: 8 SN: RT:. AA: SN: RT:.8 AA: 8 SN: L-Phenylalanine-d Cylindrospermopsin RT:. AA: 9 SN: RT: 8. AA: 89 SN: 9 RT:. AA: 898 SN: RT: 9. AA: SN: NL:.E TIC F: + c ESI SRM ms. [ ] MS ICIS Testb NL:.E TIC F: + c ESI SRM ms. [ ] MS ICIS Testb RT: 9. AA: SN: NL:.E TIC F: + c ESI SRM ms. [.99-.] MS ICIS Testb NL:.8E TIC F: + c ESI SRM ms. [ ] MS ICIS Testb 8 min Find complete method at Phenomenex.com Application ID: 9 Polar C8 Antibiotic Screen.e.e.e.e App ID 8 min Columns:. µm Polar C8 Dimension: x. mm Part No.: B-8-AN Mobile Phase: A: mm Ammonium Formate with. % Formic Acid B: Acetonitrile with. % Formic Acid.. 9 Flow Rate:. ml/min Temperature: C Detection: MS/MS (SCIEX API ). Sulfaguanidine. Sulfadiazine. Cefalexin. Lincomycin. Amoxicillin. Sulfathiazole. Sulfapyridine 8. Cefazolin 9. Sulfamerazine. Ciprofloxacin. Sulfamethazine. Sulfamonomethoxine. Enrofloxacin. Difloxacin. Chlortetracycline. Sulfamethoxazole. Florfenicol 8. Sulfaquinoxaline 9. Oxacillin. Sulfadimethoxine

16 Highly Useful Alternative to the Common C8 Polar C8 Unlike traditional UHPLC C8 stationary phases, the polar and hydrophobic versatility of Polar C8 combined with its high efficiency levels allow for enhanced separation power. Combine that with % aqueous stability and you can really see how the Luna Omega Polar C8 can potentially lead to more separation success compared to traditional C8 columns. Ketotifen Impurity Profile Beta Blockers µm Polar C8 min Thermo Scientific Hypersil GOLD.9 µm C8 min min Greater retention and resolution of impurities App ID App ID. µm Polar C8 App ID Agilent ZORBAX.8 µm SB-C8 App ID min Columns:. µm Polar C8 Hypersil GOLD.9 µm C8 Dimension: x. mm Mobile Phase: A: Water with. % Formic Acid B: Acetonitrile with. % Formic Acid.. Flow Rate:. ml/min Temperature: Ambient Detection: nm Sample: Ketotifen Clean, sharp peaks and excellent resolution Columns:. µm Polar C8 ZORBAX.8 µm SB-C8 Dimension: x. mm Mobile Phase: A: mm Postassium Phosphate ph. B: Acetonitrile. Flow Rate:. ml/min Temperature: Ambient Detection: 8 nm Sample:. Atenolol. Pindolol. Timolol. Metoprolol. Labetolol. Propranolol

17 Excellent Alternative to Existing Polar Columns Luna Omega Polar C8 is an incredibly high performing UHPLC column with a unique selectivity profile that can be used to upgrade existing methods or jumpstart new method development. Realize lower limits of detection with the increase in sensitivity levels or just utilize the efficiency gains to help resolve closely eluting peaks. Mycotoxins Screen.e.e.e.e.e.e + 8 min Conditions same for all columns: Columns:. µm Polar C8 ACQUITY HSS.8 µm T Dimension: x. mm Mobile Phase: A: Water with. % Formic Acid B: Acetonitrile with. % Formic Acid. 9. µm Polar C8 App ID.e.e.e.e.e.e + min Flow Rate:. ml/min Temperature: C Detection: MS/MS (SCIEX API ) Sample: Positive Mode (ESI+). -Acetyldeoxynivalenol. Aflatoxin G. Aflatoxin G. Aflatoxin B. Aflatoxin B. Aflatoxin M. HT Toxin 8. T Toxin 8 Waters ACQUITY HSS.8 µm T App ID Polar C8 SAMHSA Workplace Drug Testing Screen.8e.e.e.e.e 8.e.e.e.e. µm Polar C8 App ID 88 min.8e.e.e.e.e 8.e.e.e.e Waters ACQUITY HSS.8 µm T App ID 89 min Conditions same for all columns: Columns:. µm Polar C8 ACQUITY HSS.8 µm T Dimension: x. mm Mobile Phase: A: Water with. % Formic Acid B: Acetonitrile with. % Formic Acid. Flow Rate:. ml/min Temperature: C Detection: MS/MS (SCIEX API ) Sample:. Morphine. Codeine. Amphetamine. MDA. Methamphetamine. -MAM. MDMA 8. MDEA 9. BZE. PCP. THC-COOH. THC

18 Polar Case Study Catecholamines and Metabolites Polar C8 Current analytical tests for pheochromocytoma (tumor of chromaffin cells of the adrenal medulla) focus on measuring for elevated levels of plasma free metanephrine and normetanephrine, which are continually secreted by the tumors. Supplementary methods also analyze the metabolites of metanephrine and normetanephrine, including vanillylmandelic acid. With such a range of polar compounds, the aqueous stability and enhanced polar retention of the Luna Omega Polar C8, both play an strong part in generating excellent separation between all these important analytes. Catecholamines (ESI+) 8.e.e.e.e.e.e.e.e PMETs from Plasma (ESI+) 8.e.e.e.e.e.e.e.e 9 App ID 9 min App ID 9 min Catecholamine Metabolites (ESI-).e Flow Rate:. ml/min.e 9.e.e.e 8 App ID 9 min Conditions for all columns unless otherwise noted: Columns:. µm Polar C8 Dimension: x. mm Part No.: D-8-AN Mobile Phase: A: Water with. % Formic Acid B: Acetonitrile with. % Formic Acid. Flow Rate:. ml/min Temperature: C 9 Detection: MS/MS (SCIEX API ) Sample: Positive Mode (ESI+). Norepinephrine. Epinephrine. Normetanephrine. Dopamine. Metanephrine. -Methoxytyramine Negative Mode (ESI-). Vanillylmandelic acid (VMA) 8. -Hydroxyindoleacetic acid (-HIAA) 9. Homovanillic acid (HVA) Recommended Sample Preparation Method Product: Strata -X-CW Microelution 9-Well Plate Part No.: 8M-S-GA Condition: µl Methanol Equilibrate: µl Water Load Sample: µl Plasma diluted with µl Water Wash : µl Water Wash : µl : Acetonitrile/IPA Dry for minute at Hg to remove excess wash solvent Elute: x µl 8: Acetonitrile/Water with % Formic acid 8

19 Polar Case Study ETG/ETS Ethyl glucuronide (EtG) and ethyl sulfate (EtS) are metabolites of ethanol that are used by analytical laboratories to detect recent ethanol ingestion. In this application, the Luna Omega Polar C8 provides excellent separation between EtG and EtS, along with valuable separation from a urine interference peak and where other matrix suppression is common. Additionally, the high efficiency levels provided by the. µm allow for a highly sensitive, yet rapid method. ETG and ETS from Urine Sample.e.e.e.e.8e.e.e.e.e.8e.e.e.e 8.e.e.e.e.8e.e.e.e.e.8e.e.e.e 8 ETS ETG Overlaid XIC App ID 9 XIC to ETS min App ID 9 min Polar C8 9 8 XIC to ETG App ID 9 min Columns:. µm Polar C8 Dimension: x. mm Part No.: D-8-AN Mobile Phase: A: mm Ammonium Formate with. % Formic Acid B: Acetonitrile with. % Formic Acid. Flow Rate:. ml/min Temperature: C Detection: MS/MS (SCIEX API ) Sample: Negative Mode (ESI-). Urine interference. ETS. ETG 9

20 Selectivity Highlight PS C8 PS C8 Luna Omega PS C8 is a unique mixed-mode stationary phase that provides incredibly useful polar and non-polar retention. The surface of the PS C8 contains a positive charge which aids in the retention of acidic compounds through ionic interactions, while the C8 ligand promotes general reversed phase retention. This mixed-mode selectivity allows for greater separation between compounds with varying functional groups. Phase PS C8 Particle Size. µm, µm, µm Pore Size Å ph Range. - 8.* Surface Area m /g Carbon Load 9 % Pressure Limit bar USP Listing L *ph stability under gradient conditions. ph stability is.- under isocratic conditions. + TMS TMS + A C8, But More Positive PS C8 has been fine-tuned and manufactured by Phenomenex to provide a mixed selectivity that is highly useful for method development involving either combinations of polars and non-polars, or just one single compound class with small changes in functional groups. C8 silica surface PS C8 silica surface TMS TMS + TMS + + TMS

21 Enhanced Retention of Acids With its positive surface (PS) the Luna Omega PS C8 provides valuable increase in retention of acids through ionic/polar interactions. In addition, the C8 ligand also stimulates hydrophobic retention that greatly promotes increased resolution between analytes of interest. MMA and Succinic Acid.e.8e.e.e.e.e 8.e.e.e.e.e.e.e.e Organic Acids.e.e.e 9.e 8.e.e.e.e.e.e.e.e.e.e.e 9.e 8.e.e.e.e.e.e.e.e min 8 Waters ACQUITY HSS.8 μm T min 9. μm PS C8 min Greater Retention and Resolution Increased Resolution 8 App ID ACE Excel μm C8-AR App ID 9. µm PS C8 App ID 8 min 9 App ID 89 Columns:. μm PS C8 ACE Excel μm C8-AR Dimension: x. mm Mobile Phase: A: Water with. % Formic Acid B: Acetonitrile with. % Formic Acid Columns:. μm PS C8 ACQUITY HSS.8 μm T Dimension: x. mm Mobile Phase: A: Water with.% Formic Acid B: Acetonitrile with.% Formic Acid.. Flow Rate:. ml/min Temperature: C Detection: MS/MS (SCIEX API ) Sample:. Succinic acid. MMA Flow Rate:. ml/min Temperature: C Detection: MS/MS (SCIEX API ) Sample:. Lactic acid. Succinic acid. MMA. Glutaric acid. Ethylmalonic acid. Methyl succinate. Hippuric acid 8. Suberic acid 9. Sebacic acid PS C8

22 Better Peak Shape for Bases PS C8 AU AU While traditional alkyl phases are prone to show tailing for basic compounds because of secondary interactions occurring at the silica surface, the surface of the Luna Omega PS C8 was designed with positive charges that serve to repel strong basic species and consistently display sharp peak shape. Pharmaceutical Compound Mixture Better Peak Shape Waters ACQUITY BEH. μm C8. μm PS C8 App ID min App ID min Columns:. μm PS C8 ACQUITY BEH. μm C8 Dimension: x. mm Mobile Phase: A: Water with.% Formic Acid B: Acetonitrile with.% Formic Acid. 8 9 Flow Rate:. ml/min Temperature: C Detection: MS/MS (SCIEX API ) Sample:. Uracil. Pindolol. Chlorpheniramine. Nortriptyline. -Methyl--nitrobenzoic acid. -Methyl salicylaldehyde. Hexanophenone Intact Insulin.e.e.e.e.e.e.e.e.e.e.e.e Better Peak Shape. µm PS C8 Thermo Scientific Hypersil GOLD.9 µm C8 Agilent ZORBAX.8 µm SB-C8 min App ID 9, 9 and 9 Columns:. μm PS C8 Hypersil GOLD.9 μm C8 ZORBAX.8 µm SB-C8 Dimension: x. mm Mobile Phase: A: Water with.% Formic Acid B: Acetonitrile with.% Formic Acid. 8 Flow Rate:. ml/min Temperature: C Detection: MS/MS (SCIEX API ) Sample: Insulin

23 Excellent Loadability AU The combination of great surface coverage and novel surface chemistry results in narrow peak shapes for an extended amount of sample loaded on the Luna Omega PS C8. This can visually be seen in the examples below where the excellent peak shape at different loads is kept relatively constant on the PS C8, while more conventional UHPLC columns show tailing and much greater peak broadening as more sample is loaded. Amitriptyline µg Load mau 8 Amitriptyline Loading Study μm PS C8 Supelco Titan.9 µm C8 AMT HALO µm C8... min Better Peak Shape Over All Loads App ID min. µm PS C8 App ID Columns:. μm PS C8 Titan.9 µm C8 HALO µm C8 Dimension: x. mm Mobile Phase: Water with.% Formic Acid / Acetonitrile with.% Formic Acid (8:) Flow Rate:. ml/min Temperature: C Detection: nm Sample: Amitriptyline Columns:. μm PS C8 ACQUITY BEH. μm C8 Dimension: x. mm Mobile Phase: A: Water with.% Formic Acid B: Acetonitrile with.% Formic Acid Flow Rate:. ml/min Temperature: C Detection: nm Sample: Amitriptyline 8 PS C8 AU Waters ACQUITY BEH. μm C8... min App ID 8 Peak Width at % PS C8 and Competitors x. mm µg on column App ID 9 Better Peak Shape Over All Loads Luna. µm PS C8 HALO µm C8 ACQUITY BEH. µm C8 Hypersil GOLD.9 µm C8

24 Method Development Flexibility PS C8 The combination of available Luna Omega stationary phases represent an outstanding tool set for the separation of acids, bases, neutrals or mixtures. Use the C8 to focus on hydrophobic interactions or the mixedmode functionality of the Polar C8 and PS C8 to get enhanced retention of both polars and non-polars. Pharmaceutical Drug Impurity Profile Useful alternative peak elution order. μm PS C8 8 9 min App ID. μm Polar C8 App ID 8 9 min μm C8 8 9 min App ID Columns:. μm PS C8. μm Polar C8. μm C8 Dimension: x. mm Mobile Phase: A: Water with.% Formic Acid B: Acetonitrile with.% Formic Acid Flow Rate:. ml/min Temperature: C Detection: nm Sample: Proprietary drug impurity profile

25 Improving Existing Basic Compound Methods Luna Omega PS C8 is a brilliant UHPLC stationary phase with a distinctive selectivity profile that can spur new method development. At the same time, this versatile phase can also upgrade existing methods by way of increases in sensitivity and efficiency levels to help resolve closely eluting peaks. Peptides by LC/MS.e.e.e.e 9.e 8.e.e.e.e.e.e.e.e.e.e.e.e 9.e 8.e.e.e.e.e.e.e.e Beta-Blockers μm PS C8 -. min μm PS C8 Waters ACQUITY CSH. µm C8 min -. min App ID App ID.e.e.e.e 9.e 8.e Better Sensitivity Luna Omega.e min.e ACQUITY.e CSH.e.e.e.e App ID App ID App ID Columns:. μm PS C8 ACQUITY CSH. µm C8 Dimension: x. mm Mobile Phase: A: Water with.% Formic Acid B: Acetonitrile with.% Formic Acid min Waters CORTECS. μm C8+. 9 Flow Rate:. ml/min Temperature: C Detection: MS/MS (SCIEX API ) Sample:. Angiotensin II. Met-Enkephalin. Leu-Enkephalin Columns:. μm PS C8 CORTECS. μm C8+ Dimension: x. mm Mobile Phase: A: mm Ammonium Formate ph. B: Acetonitrile Flow Rate:. ml/min Temperature: C Detection: 8 nm Sample:. Pindolol. Timolol. Metoprolol. Labetolol. Propranolol PS C8

26 Use Luna and Phases Together Combine the new Luna Omega µm Polar C8 and PS C8 with Luna µm phases like Phenyl-Hexyl and PFP() to expand your HPLC method development choices or try different separation modes with Luna phases such as Silica(), NH, and HILIC. Ezetimibe Degradation Profile mau 8 - mau Luna μm PFP() min μm Polar C8 App ID 9 Columns: Luna μm PFP() μm Polar C8 Luna μm Phenyl-Hexyl Luna μm C8() Dimension: x. mm Mobile Phase: A: Water B: Acetonitrile Flow Rate: ml/min Temperature: C Detection: nm Sample: Ezetimibe Degradation Profile 8 App ID 98 - min mau Luna μm Phenyl-Hexyl 8 App ID 99 - min mau Luna μm C8() 8 App ID 8 - min

27 UHPLC to HPLC to PREP Scalability With direct selectivity scalability from Luna Omega. µm to µm you can fluidly transfer methods from UHPLC platforms to HPLC and preparative instrumentation. Additionally, you can easily go in reverse and use a. µm to analyze fractions taken from a µm preparative column. Direct Scalability. µm to µm mau. μm Polar C8 μm Polar C8 min App ID Columns:. μm Polar C8 µm Polar C8 Dimension: x. mm Mobile Phase: A: Water with.% Formic Acid B: Acetonitrile with.% Formic Acid 9 Flow Rate:. ml/min Temperature: C Detection: nm Sample:. Uracil. Pindolol. Chlorpheniramine. Nortriptyline. -Methyl--nitrobenzoic acid. -Methyl salicylaldehyde. Hexanophenone UHPLC to HPLC to PREP Analyze Prep Fractions via UHPLC UHPLC Scout min Dimension: x. mm Flow Rate:. ml/min Preparative Purification mau 8 Dimension: x. mm Flow Rate: ml/min 8 App ID App ID Optimized HPLC mau 8 8 Dimension: x. mm Flow Rate:. ml/min App ID Conditions for all columns (as noted): Columns: μm PS C8 Mobile Phase: A: Water with.% TFA B: Acetonitrile with.% TFA Temperature: C Detection: nm Sample:. Impurity. Proprietary API. Impurity 9

28 Complementary UHPLC Work Horses Combine the versatile interaction mechanisms of the Luna Omega C8 and Polar C8 to achieve successful separations and improve upon challenging existing methods. Natural Cannabinoids mau mau µm Polar C8 8 9 min Greater hydrophobic retention App ID 8. µm C8 App ID 8 Columns:. µm Polar C8. µm C8 Dimension: x. mm Mobile Phase: A: mm Ammonium Formate ph. B: Acetonitrile. 9 9 Flow Rate:. ml/min Temperature: C Detection: nm Sample:. CBDV. Cannabidiol. CBG. Cannabidolic Acid. CBG-A. Cannabinol. Delta 9 THC 8. Delta 8 THC 9. CBC. THCA-A 8 9 min Influence Selectivity with % Aqueous Stability and Polar Interactions Intact Peptides mau mau. µm Polar C8. µm C8 App ID min Use the % aqueous stability of polar C8 to aid in better retention of polar compounds App ID 9 Conditions for all columns except where noted: Columns:. µm Polar C8. µm C8 Dimension: x. mm Mobile Phase: A: Water with. % TFA B: Acetonitrile with. % TFA Flow Rate:. ml/min Temperature: C Detection: nm Sample:. Gly-Tyr. Val-Tyr-Val. Met-Enkephalin. Leu-Enkephalin. Angiotensin II min 8

29 Complementary UHPLC Work Horses For the analysis of a large screen or mixture of compounds, you can benefit greatly from utilizing both the Luna Omega C8 and Polar C8 during method development. The strong focused hydrophobic retention of the C8 will provide excellent non-polar retention and separation, while the Polar C8 provides a complementary hydrophobic/polar combined selectivity that can retain and separate typically early eluting compounds Tryptic Digest - Peptide Map of BSA.e9.e9.e9.e9 9.e8 8.e8.e8.e8.e8.e8.e8.e8.e8 8. µm Polar C min App ID 99 Greater resolution of early, more polar eluters.e8.e8 8.e8.e8.e8. µm Polar C8 App ID.e8.e8 8.e8.e8.e8. µm C8 App ID.e8.e8 8 9 min 8 9 min Better resolution of more hydrophobic compounds.e9.e9 9.e8 8.e8.e8.e8.e8.e8.e8.e8.e8.. µm C8 min App ID Conditions same for all columns: Columns:. µm C8. µm Polar C8 Dimension: x. mm Mobile Phase: A:. % Formic Acid in Water B:. % Formic Acid in Acetonitrile. Flow Rate:. ml/min Temperature: C Detection: MS/MS (SCIEX API ) Sample: Digested BSA 9

30 + Kinetex = Happy UHPLC Increase your method development options by screening a combination of core-shell and fully porous selectivities. Natural Cannabinoids mau mau mau Excellent resolution 8 9 min Faster analysis with similar peak order Alternative peak elution order Luna Omega. µm Polar C8 8 9 min App ID 8 App ID 8 App ID 8 Kinetex. µm F Kinetex. µm Biphenyl TMS TMS Polar Polar F F F F F TMS TMS F TMS TMS Biphenyl 8 9 min Columns:. µm Polar C8 Kinetex. µm Biphenyl Kinetex. µm F Dimension: x. mm Mobile Phase: A: mm Ammonium Formate ph. B: Acetonitrile. 9 9 Flow Rate:. ml/min Temperature: C Detection: nm Sample:. CBDV. Cannabidiol. CBG. Cannabidolic Acid. CBG-A. Cannabinol. Delta 9 THC 8. Delta 8 THC 9. CBC. THCA-A

31 + Kinetex = Happy UHPLC Combining orthogonal UHPLC particle morphologies and selectivities like the C8, Kinetex Biphenyl, and Kinetex EVO C8 will drastically increase your probability of separation success! Morphine Drugs of Abuse Analysis Luna Omega. µm C8 ph. ADVANTAGE Excellent inertness and strong hydrophobic retention Kinetex. µm Biphenyl Kinetex. µm EVO C8 App ID App ID min ph. ADVANTAGE Extra aromatic (pi-pi) and dipole-dipole retention App ID min ph 8. ADVANTAGE Excellent peak shape and retention of bases at high ph min Conditions for all columns except where noted: Columns:. µm C8 Kinetex. µm Biphenyl Kinetex. µm EVO C8 Dimension: x. mm Mobile Phase:. µm C8 and Kinetex. µm Biphenyl A:. % Formic Acid in Water B:. % Formic Acid in Acetonitrile Kinetex. µm EVO C8 A: mm Ammonium Bicarbonate ph B: Acetonitrile. 9 9 Flow Rate:. ml/min Temperature: C Detection: MS/MS (SCIEX API ) Sample: Drugs of Abuse

32 Excellent Lifetime. µm media was designed to be durable and withstand the high pressures and rigors of UHPLC work in combination with providing excellent performance. Accelerated Lifetime Study.% 9.% 8.%.% % Increase over Initial Backpressure.%.%.%.%.%.%.% Injection Cycle App ID Luna Omega. µm C8 (AVG) Waters ACQUITY BEH. µm C8 (AVG) Conditions same for both columns: Columns:. µm C8 ACQUITY BEH. µm C8 Dimension: x. mm Mobile Phase: A:. % Formic Acid in Water B:. % Formic Acid in Acetonitrile. Flow Rate:. ml/min Temperature: C Detection: nm Sample: Protein Matrix 9 Extend UHPLC Column Lifetime Even More with SecurityGuard ULTRA See page for details

33 Sample Preparation Further Improve Lifetime with Sample Preparation Both UHPLC instrumentation and columns can benefit from consistent removal of microparticulates/contaminants that may appear in solvents, samples or specific matrices. Over time these problematic compounds can build up on instrumentation and columns, leading to the need for costly maintenance and repair. Strata-X polymeric SPE, Novum liquid extraction, and Phenex syringe filters are excellent options to try. Polymeric SPE Very targeted form of sample preparation that allows you to isolate your analyte of interest while removing any interfering compounds in your sample StrataX novum simplified liquid extraction Remove interferences such as proteins, phospholipids, salts, and more in a quick and simple procedure Novum Convenient and inexpensive way to remove particulates from LC samples Phenex

34 Protect any UHPLC Column Protect your UHPLC column, including Luna Omega. µm columns, from damaging contaminants and microparticulates with the SecurityGuard ULTRA guard cartridge system! Simple to use Extend column lifetime Pressure rated to, psi (,8 bar) Fits virtually all manufacturers columns. to. mm ID ULTRA UHPLC Column Protection From Injector SecurityGuard ULTRA UHPLC Column to Detector Holder Cartridge Contaminants Trapped SecurityGuard ULTRA Guard Cartridge System (Cartridge connected to Holder) Compounds of Interest Contaminants and Particulates Frit * Cartridge schematic not drawn to scale Material Chamber See it in action:

35 Protect any HPLC Column The easiest way to extend column performance is to prevent contaminants and particulates from getting into your Luna Omega µm column with the SecurityGuard Standard guard cartridge system. Protects and extends column lifetimes Virtually no change in chromatography Simple to use HPLC Column Protection From Injector Fingertight connection to virtually any female inverted endfitting worldwide 9 Cartridge Replaced 8 Guard Cartridge System HPLC Column to Detector A B Contaminants Trapped A B Cutaway view showing cartridge can be easily inspected for contaminants Universal Fingertight connection to HPLC column no wrenches required contaminants compounds of interest Backpressure (Bar) - Injections Column returns to initial backpressure Accelerated lifetime test using endogenous biomolecule matrix on a µm x. mm column with SecurityGuard cartridges. Backpressure values represent additional backpressure contributed by SecurityGuard. The SecurityGuard analytical cartridge holder (patented) directly finger-tightens into virtually any manufacturer s column endfitting. Contaminants are retained by an inexpensive disposable cartridge instead of damaging your valuable HPLC and SFC column investment. Simply replace SecurityGuard cartridges instead of your expensive columns. In this graph, once the expired SecurityGuard cartridge was replaced, the pressure immediately dropped and the column performance was restored allowing for extended column use. See it in action:

36 Luna and Column Characteristics Luna Omega Phases Description Particle Sizes (µm) Pore Size (Å) Surface Area (m /g) Carbon Load (%) ph Stability C8 C8 ligand optimized for improved * peak shape Polar C8 Enhanced selectivity/retention for.,, * polar analytes without diminishing useful non-polar retention PS C8 Mixed-mode functionality offering.,, * enhanced retention of polar acids along with improved peak shape for strong bases * ph stability under gradient conditions. ph stability is. - under isocratic conditions. Reversed Phase Normal Phase HILIC IEX Luna Phases Description Particle Sizes (µm) Silica() Unbonded silica,,, -PREP, Pore Size (Å) Surface Area (m/g) Carbon Load (%) ph Stability. -. C Carbon ligand, * C8() C8() C8 ligand optimized for improved peak shape C8 ligand optimized for improved peak shape,,, -PREP,.,,,, -PREP, * * CN Versatile CN phase,,.. -. NH Rugged and reproducible NH,, Phenyl-Hexyl Phenyl phase attached to C (hexyl) ligand,,, -PREP, * SCX Benzene sulfonic acid, Binding. -. Capacity:. meq/g HILIC Reproducible, cross-linked diol, PFP() Pentafluorophenyl with a C (propyl) linkage, * ph range is. - 9 under gradient conditions. ph range is. - under isocratic conditions. Reversed Phase Normal Phase HILIC IEX Find more information on all these phases online at

37 Ordering Information Buy Luna Omega Now. μm Microbore Columns (mm) Phases x. x. x. Polar C8 B-8-A D-8-A F-8-A C8 B--A D--A F--A SecurityGuard. μm Minibore Columns (mm) ULTRA Cartridges Phases x. x. x. x. /pk Polar C8 A-8-AN B-8-AN D-8-AN F-8-AN AJ-9 PS C8 A--AN B--AN D--AN F--AN AJ-98 C8 A--AN B--AN D--AN F--AN AJ-9 for. mm ID SecurityGuard µm Minibore and MidBore Columns (mm) Cartridges (mm) Phases x. x. x. x. x. x. x. x.* / pk Polar C8 A--AN B--AN D--AN F--AN B--Y D--Y F--Y AJ- PS C8 A-8-AN B-8-AN D-8-AN F-8-AN B-8-Y D-8-Y F-8-Y AJ- for ID:.-. mm SecurityGuard µm Analytical Columns (mm) Cartridges (mm) Phases x. x. x. x. x.* / pk Polar C8 B--E D--E F--E G--E AJ- PS C8 B-8-E D-8-E F-8-E G-8-E AJ- for ID:.-8. mm SecurityGuard µm Minibore and MidBore Columns (mm) Cartridges (mm) Phases x. x. x. x. x. x. x. x.* / pk Polar C8 A--AN B--AN D--AN F--AN B--Y D--Y F--Y AJ- PS C8 A--AN B--AN D--AN F--AN B--Y D--Y F--Y AJ- for ID:. -. mm SecurityGuard μm Analytical Columns (mm) Cartridges (mm) Phases x. x. x. x. x.* / pk Polar C8 B--E D--E F--E G--E AJ- PS C8 B--E D--E F--E G--E AJ- for ID:.-8. mm SecurityGuard μm Axia Packed Preparative Columns (mm) Cartridges (mm) Phases x. x. x x x x.** x. /ea /ea Polar C8 F--P-AX G--P-AX F--U-AX G--U-AX G--V-AX AJ- AJ- PS C8 F--P-AX G--P-AX F--U-AX G--U-AX G--V-AX AJ-8 AJ-9 for ID:. mm for ID: mm SecurityGuard ULTRA Cartridges require holder, Part No.: AJ-9 * SecurityGuard Analytical Cartridges require holder, Part No.: KJ-8 ** PREP SecurityGuard Cartridges require holder, Part No.: AJ-8 PREP SecurityGuard Cartridges require holder, Part No.: AJ-8 If Luna analytical columns do not provide at least an equivalent separation as compared to a competing column of the same particle size, similar phase and dimensions, return the column with comparative data within days FOR A FULL REFUND. Phenomenex l WEB:

38 Ordering Information Buy Luna Now SecurityGuard μm Microbore and Minibore Columns (mm) Cartridges (mm) Phases x. x. x. x. x. x. x. x.* / pk Silica() A--B B--B F--B G--B AJ- C A--B B--B F--B AJ-9 C8 () F-9-A A-9-B B-9-B F-9-B G-9-B AJ-89 C8 () B--A F--A G--A A--B B--B F--B G--B AJ-8 CN B--B F--B AJ- Phenyl-Hexyl B--A A--B B--B F--B G--B AJ- NH B-8-A F-8-A A-8-B B-8-B F-8-B G-8-B AJ- PFP() A-8-B B-8-B F-8-B AJ-8 for ID:. -. mm SecurityGuard μm MidBore Columns (mm) Cartridges (mm) Phases x. x. x. x. x.* / pk Silica() B--Y F--Y AJ- C F--Y AJ-9 C8() A-9-Y B-9-Y F-9-Y G-9-Y AJ-89 C8() A--Y B--Y F--Y G--Y AJ-8 CN B--Y F--Y G--Y AJ- Phenyl-Hexyl B--Y F--Y G--Y AJ- NH B-8-Y F-8-Y G-8-Y AJ- SCX F-98-Y AJ-8 HILIC F--Y AJ-88 PFP() B-8-Y F-8-Y AJ-8 for ID:. -. mm SecurityGuard μm Analytical Columns (mm) Cartridges (mm) Phases x. x. x. x.* / pk Silica() B--E AJ-8 C B--E AJ-9 C8() A-9-E B-9-E C-9-E AJ-9 C8() A--E B--E C--E AJ-8 CN A--E B--E C--E AJ- Phenyl-Hexyl A--E B--E AJ- NH B-8-E AJ- SCX B-98-E AJ-8 HILIC AJ-89 PFP() B-8-E AJ-8 for ID:.-8. mm *SecurityGuard Analytical Cartridges require holder, Part No.: KJ-8 Find. µm and µm Luna column options at 8 Phenomenex l WEB:

39 Ordering Information Buy Luna Now μm Analytical and Semi-Prep Columns (mm) SecurityGuard Cartridges (mm) Phases x. x. x. x x. * x / pk / pk Silica() D--E F--E G--E G--N AJ-8 AJ- C D--E F--E G--E G--N AJ-9 AJO- C8() D-9-E F-9-E G-9-E G-9-N AJ-9 AJ- C8() D--E F--E G--E G--N AJ-8 AJ- CN D--E F--E G--E G--N AJ- AJ- Phenyl-Hexyl D--E F--E G--E G--N AJ- AJ- NH D-8-E F-8-E G-8-E G-8-N AJ- AJ- SCX D-98-E F-98-E G-98-E G-98-N AJ-8 AJ-9 HILIC D--E F--E G--E G--N AJ-89 AJ-89 PFP() D-8-E F-8-E G-8-E G-8-N AJ-8 AJ-8 for ID: mm 9 - mm SecurityGuard μm Axia Packed Preparative Columns (mm) Cartridges (mm) Phases x. x. x. x. x. ** /ea Silica() D--P-AX F--P-AX G--P-AX AJ-9 C G--P-AX C8() F-9-P-AX G-9-P-AX AJ-8 C8() B--P-AX D--P-AX F--P-AX G--P-AX AJ-89 CN G--P-AX AJ-8 Phenyl-Hexyl D--P-AX F--P-AX G--P-AX AJ-8 NH F-8-P-AX G-8-P-AX AJ-8 PFP() D-8-P-AX F-8-P-AX G-8-P-AX AJ-8 HILIC D--P-AX F--P-AX G--P-AX AJ-889 for ID: 8-9 mm SecurityGuard μm Axia Packed Preparative Columns (mm) (cont'd) Cartridges (mm) Phases x x x x /ea Silica() G--U-AX AJ-8 C8() D-9-U-AX AJ-8 C8() B--U-AX D--U-AX G--U-AX AJ-8 Phenyl-Hexyl G--U-AX AJ-8 PFP() D-8-U-AX AJ-88 HILIC G--U-AX AJ-88 for ID: - 9 mm * SecurityGuard Analytical Cartridges require holder, Part No.: KJ-8 SemiPrep SecurityGuard Cartridges require holder, Part No.: AJ-98 ** PREP SecurityGuard Cartridges require holder, Part No.: AJ-8 PREP SecurityGuard Cartridges require holder, Part No.: AJ-8 Phenomenex l WEB: 9

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