Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK

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1 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Cellular Pathology St James' University Hospital eckett Street Leeds LS9 7TF Contact: Michelle Eager Tel: +44 (0) Fax: +44 (0) Website: CellularPathology.aspx Laboratory locations: details ctivity code ddress exley Wing St James' University Hospital eckett Street Leeds LS9 7TF Local contact s above Routine diagnostic service for histopathology (including neuropathology and paediatric pathology) Reporting of the above and non gynae cytology Out of hours on call service for urgent frozen sections / diagnosis ddress Chancellors Wing St James' University Hospital eckett Street Leeds LS9 7TF Local contact s above Gynaecological and non-gynaecological cytology Reporting of the above Muscle / nerve histopathology, enzyme histochemistry ddress Microbiology Department Leeds General Infirmary Great George Street Leeds LS1 3EX Local contact s above HPV testing C ddress Seacroft Hospital York Road Leeds West Yorkshire LS14 6UH Local contact s above ndrology (post vasectomy and infertility investigation). D ssessment Manager: LD1 Page 1 of 29

2 United Kingdom ccreditation Ser vice dditional site activity performed away from the laboratory details ctivities On-site Mortuary and ody Store, St James' University Hospital On-site Mortuary and ody Store, Leeds General Infirmary Storage of bodies Viewing facilities Record keeping (body identification and audit trail) ssessment Manager: LD1 Page 2 of 29

3 DETIL OF CCREDITTION HUMN ODY TISSUE ND FLUIDS of diagnosis Cervical/vaginal cells (Liquid ased Cytology/LC sample) Cervical Cytopathology Specimen Processing, slide preparation and stain (Papanicolaou: HPSOP CY0081 SurePath Prepmate HPSOPCY 0202 LC Sample Preparation using D Totalys Multiprocessor HPSOPCY0198 Use of SlidePrep HPSOPCY0085 Leica CV5030 Coverslipper Slides prepared in house as above Morphological assessment and interpretation/diagnosis HPSOPCY0026 Leica DM2000 Microscope HPSOPCY0164 Screening Liquid ased Cytology slides HPSOPCY0169 Checking Liquid ased Cytology Slides HPSOPCY0144 The Medical Reporting and management of Gynaecological Cytology Samples Cervical/vaginal cells (Liquid ased Cytology/LC sample) Detection of HR-HPV HPSOPCY0166 HPV testing: liquoting of LC samples MOPHPV02 HPV PCR/ Genotyping detection using GEN- PROE Panther C HPSOPCY0167 Reporting of HPV results ssessment Manager: LD1 Page 3 of 29

4 HUMN ODY TISSUE ND FLUIDS (cont d) ody fluids / Fine needle aspirations, urines and bronchial/ sputum samples Diagnostic Non Gynae Cytopathology Sample preparation (Rotina 380 centrifuge,, Shandon cytospin 4): HPSOP 0199 Preparation of Non Gynae Samples HPSOPCY0020 Preparation of Cell blocks HPSOP 0199 Preparation of Non Gynae Samples Visualisation of nuclear detail in cells present HPSOPCY0024 H&E (automated using Jung utostainer) HPSOPCY0086 Papanicolaou (automated using Jung utostainer) HPSOPCY0132 Hema-Gurr Diff Quick Stain (manual) Slides prepared in house as above Morphological assessment and interpretation/diagnosis HPSOP0143 Medical reporting of Non-Gynae cytology ssessment Manager: LD1 Page 4 of 29

5 HUMN ODY TISSUE ND FLUIDS (cont d) Tissue / organs Histopathology Tissue dissection: HPSOP 0300 Cut Up of Specimens by iomedical Scientists HPSOP 0305 Cut Up of Specimens by Medical Staff HPSOP 0317 Guidelines For Inflating Lung HPSOP 0217 Handling of CJD Specimens HPSOP 0325 Further locks HPSOP 0320 Guidelines for Opening a Fresh Uterus HPSOP 0315 Guidelines on Opening a Kidney HPSOP 0227 Renal iopsy Procedure HPSOPMDLG2 009 Dissection of gall bladder resection specimens HPSOPMDLG2 010 Dissection of appendiceal resection specimens HPSOPMDLG3 001 Examination of high risk infective group 3 specimens HPSOPMDLGYN 001 Cut up of gynae specimens ssessment Manager: LD1 Page 5 of 29

6 HUMN ODY TISSUE ND FLUIDS (cont d) Tissue / organs (cont d) Histopathology (cont d) Tissue dissection: HPSOPMDLPd 001 Multicolour injection examination of monochorionic twin placentas (twin-twin transfusion syndrome) HPSOPMDLPd 003 Operations for Hirschsprung s Disease and Other Motility bnormalities HPSOPMDLPd 004 Handling paediatric tumours in the laboratory HPSOPMDLPd 006 Reporting of placental examination (unaccompanied by a fetus) X-ray/photography (reast cases only) (Faxitron): HPSOP 0360 Decalcification: HPSOP 0330 Decalcification of Specimens HPSOP 1626 Ex-Decal Specimens for Processing HPSOP 1622 X-raying of Decalcification Samples HPSOP 1632 Chemical End Point Testing of Decalcification Samples HPSOP 1624 Ground Section Preparation ssessment Manager: LD1 Page 6 of 29

7 HUMN ODY TISSUE ND FLUIDS (cont d) Tissue / organs (cont d) Histopathology (cont d) Tissue processing: HPSOP 441 Processing tissue using the Leica Peloris tissue processor (routine & big blocks & long process) HPSOP 410 Processing tissue using the Citadel 2000 (small urgent biopsies and neuropath specimens) Embedding: HP SOP 425 Embedding paraffin wax impregnated tissues Microtomy: HPSOP 502 Sectioning using rotary microtome HPSOP 550 Sectioning using automated microtome HPSOP 0504 Sectioning of Problem locks HPSOP 505 Sections/levels required for particular tissue types Frozen Sections: HPSOP 1609 Leica 1950 cryostat HPSOP 1614 Specimens Requiring Frozen Sections HPSOP 1603 Preparation of Split Skin iopsies for Immunofluorescence ssessment Manager: LD1 Page 7 of 29

8 HUMN ODY TISSUE ND FLUIDS (cont d) Tissue / organs (cont d) Histopathology (cont d) Frozen Sections HPSOP 1602 Processing and Embedding of lopecia Cases and other Paraffins HPSOP 1105 Protocol for Transplant Donor Lesion iopsies Frozen Sections Tissue Identification of basophilic and eosinophilic structures Routine Haematoxylin and Eosin Staining utomated: HP SOP 511 Use and maintenance of Leica utostainer/coverslipper Manual: HPSOP 1604 Mayers haematoxylin and eosin for rapid method for frozen sections Tissue Histopathology - Identification of: utomated: HPSOP 657 Use of the Dako rtisan Link Pro for Special stain techniques cid and neutral mucins PS Diastase resistance acidic and neutral mucins Mucins, glycogen PS+D PS Diastase resistance mucin DPS asement membranes, fungi ExPS ssessment Manager: LD1 Page 8 of 29

9 HUMN ODY TISSUE ND FLUIDS (cont d) Tissue (cont d)_ Histopathology - Identification of: (cont d) utomated: HPSOP 657 Use of the Dako rtisan Link Pro for Special stain techniques asement membranes, fungi ExDPS Fungi Grocott Iron (ferric) Perls Reticulin fibers Retic Spirochettes, H.Pylori Warthin & Starry myloid Congo Red Manual staining: Copper/Hepatitis Orcein Sulphated acid mucins HPSOP 607 lcian blue ph1.0 cid mucins HPSOP 608 lcian blue ph2.5 cid mucins/connective tissue HPSOP 609 lcian blue van gieson cid mucins/connective tissue/elastic HPSOP 653 van gieson lcian blue elastic L amyloid HPSOP 611 Congo red potassium permanganate Nissl granules and neurones HPSOP 612 Cresyl violet Elastic/connective tissue HPSOP 613 Elastic van gieson ssessment Manager: LD1 Page 9 of 29

10 HUMN ODY TISSUE ND FLUIDS (cont d) Tissue (cont d) Histopathology - Identification of (cont d): Manual special staining: DN HPSOP 614 Feulgen for DN ile pigment HPSOP 615 Fouchets H Pylori HPSOP 616 Giemsa- modified one marrow HPSOP 617 Giemsa- overnight Gram positive bacteria HPSOP 618 Grams technique Gram positive and negative bacteria HPSOP 619 Gram twort Neuroendocrine granules HPSOP 620 Grimelius General tissue morphology HPSOP 622 Mayers Haematoxylin and Eosin Collagen/muscle HPSOP 659 Haematoxylin picro sirius red Collagen/muscle HPSOP 625 Haematoxylin van gieson cid mucin HPSOP 651 Hales colloidal iron Myelin HPSOP 627 Luxol fast blue Haematoxylin and Eosin Melanin HPSOP 628 Masson Fontana Muscle/collagen HPSOP 629 Masson Trichrome Paired sections identifies melanin pigment HPSOP 630 Melanin bleach ssessment Manager: LD1 Page 10 of 29

11 HUMN ODY TISSUE ND FLUIDS (cont d) Tissue (cont d) Histopathology - Identification of (cont d): Manual special staining: Fibrin/connective tissue HPSOP 631 MS Copper HPSOP 639 Rhodanine technique Melanin HPSOP 640 Schmorls technique Fat HPSOP 643 Sudan IV myloid HPSOP 644 Thioflavine T Mast cells HPSOP 645 Toluidine blue Calcium HPSOP 646 Von Kossa Mycobacterium leprae HPSOP 647 Wade fite Tubercle bacilli HPSOP 649 Ziehl Neelsen Lipids HPSOP 643 Sudan IV staining (frozen sections only) ssessment Manager: LD1 Page 11 of 29

12 HUMN ODY TISSUE ND FLUIDS (cont d) Tissue and cell blocks Histopathology identification of: utomated Immunohistochemical staining, Dako PTLink/utostainer HPSOP 712 Preparation of Primary antibodies and associated reagents HPSOP 715 DKO utostainer Link 48 HPSOP 716 DKO PTLinks for antigen retrieval Using the following monoclonal, polyclonal and cocktail antibodies: 1-T deiciency 1-T Pituitary hormone CTH Epithelial marker E1/3 Primary liver tumours/yolk sac tumours FP Prostate cancer MCR myloid deposits MYLOID- Prostate/breast marker NDROGEN R Lewy bodies /Parkinsons disease -SYN Neuronal damage PP Marker for apoptosis CL-2 Epithelial marker ER-EP4 Senile plaques in lzheimers disease -MYLOID ssessment Manager: LD1 Page 12 of 29

13 HUMN ODY TISSUE ND FLUIDS (cont d) Tissue and cell blocks (cont d) Histopathology identification of (cont d): utomated Immunohistochemical staining, Dako PTLink/utostainer using the following monoclonal, polyclonal and cocktail antibodies: Fibromatous lesions -CTENIN Germ cell tumours -HCG Marker of breast tumours RST-2 (GCDFP15) Marker of ovarian tumours C125 Tumours of GI tract and pancreas C19.9 Marker of thyroid tumours CLCITONIN Marker of smooth nuscle CLDES Marker for mesothelioma CLRET Epithelial marker CM 5.2 Marker of rejection in renal/liver transplants Langerhan cells T lymphocytes T helper lymphocytes T supressor lymphocytes C4D CD1 CD3 CD4 CD8 ssessment Manager: LD1 Page 13 of 29

14 HUMN ODY TISSUE ND FLUIDS (cont d) Tissue and cell blocks (cont d) Histopathology identification of (cont d): utomated Immunohistochemical staining, Dako PTLink/utostainer using the following monoclonal, polyclonal and cocktail antibodies: Lymphoid/endometrial malignancies CD10 Hogkins lymphoma CD15 lymphocytes CD20 Lymphoma marker CD21 Hogkins lymphoma CD30 Endothelial marker CD31 Endothelial marker CD34 Lymphocyte marker CD45 Marker of nerve/neuroendocrine tumours CD56 Histiocytes/macrophages CD68 lymphocytes CD79a Ewings sarcoma CD99 Mesothelioma marker CD141 Histiocytes/macrophages CD163 GI tract tumours CDX2 ssessment Manager: LD1 Page 14 of 29

15 HUMN ODY TISSUE ND FLUIDS (cont d) Tissue and cell blocks (cont d) Histopathology identification of (cont d): utomated Immunohistochemical staining, Dako PTLink/utostainer using the following monoclonal, polyclonal and cocktail antibodies: Epithelial marker CE-M Epithelial marker/liver tumour marker CE-P Neuroendocrine cells CHROMO Epithelial marker CK5 Epithelial marker CK5/6 Epithelial marker CK7 Epithelial marker/mallory bodies CK8/18 Epithelial marker CK14 Epithelial marker CK19 Epithelial marker CK20 Gastrointestinal stromal tumour marker C-KIT (CD117) Cytomegalovirus infection CMV Marker of basement membrane COLL IV Marker of lymphatic channels D2-40 ssessment Manager: LD1 Page 15 of 29

16 HUMN ODY TISSUE ND FLUIDS (cont d) Tissue and cell blocks (cont d) Histopathology identification of (cont d): utomated Immunohistochemical staining, Dako PTLink/utostainer using the following monoclonal, polyclonal and cocktail antibodies: Pan muscle marker DES Gastrointestinal stromal tumour marker DOG-1 Cell adhesion molecule E-CD Epidermal growth factor receptor EGFR Epithelial marker EM Estrogen receptor/ prognostic breast marker ER Vascular tumours ERG Pituitary hormone FSH Marker of gastrin producing tumours GSTRIN Glial cells benign and malignant GFP Pituitary hormone GH Pancreatic tumour typing Glucagon Pancreatic tumour typing Glucagon Hepatitis infected cells HEP c Hepatitis infected cells HEP s ssessment Manager: LD1 Page 16 of 29

17 HUMN ODY TISSUE ND FLUIDS (cont d) Tissue and cell blocks (cont d) Histopathology identification of (cont d): utomated Immunohistochemical staining, Dako PTLink/utostainer using the following monoclonal, polyclonal and cocktail antibodies: Marker of normal/malignant hepatocytes HEPR 1 Marker of Her2 amplification in breast tumours. HER 2 Melanoma marker HM45 Epithelial marker HMC Marker for H pylori HP Marker for teratoma HPL Herpes simlex 1 infection HSV 1 Lymphoma markers Granulosa cell tumours Mucinous epithelial ovarian tumours Pancreatic tumours Lymphoma marker Proliferation marker Lymphoma marker Pituitary hormone Ig IgG IgM Inhibin Insulin KPP KI67 Lambda LH ssessment Manager: LD1 Page 17 of 29

18 HUMN ODY TISSUE ND FLUIDS (cont d) Tissue and cell blocks (cont d) Histopathology identification of (cont d): utomated Immunohistochemical staining, Dako PTLink/utostainer using the following monoclonal, polyclonal and cocktail antibodies: Melanoma marker MEL- Mismatch repair protein MLH 1 MSH 2 MSH6 Epithelial marker MUC4 in tumours MNF MUC4 Marker for rhabdomyosarcoma MYOD 1 Marker for rhabdomyosarcoma MYOGENIN Neuronal marker Marker of neuroblastoma Neuronal marker Neuronal marker Neuroectodermal marker Epithelial gynae tumours Marker of CIN Marker of wild type P53 in malignancy Marker of molar pregnancy N52 N84 NEU N NFP NSE PX-8 P16 P53 p57 ssessment Manager: LD1 Page 18 of 29

19 HUMN ODY TISSUE ND FLUIDS (cont d) Tissue and cell blocks (cont d) Histopathology identification of (cont d): utomated Immunohistochemical staining, Dako PTLink/utostainer using the following monoclonal, polyclonal and cocktail antibodies: Marker in breast/prostate tumours p63 Infection with parvo virus PRVO Marker of germ cell tumours PLP Mismatch repair protein PMS2 Polyoma virus infection I renal transplant patients POLYOM SV40 Pituitary hormone PROLCTIN Progesterone receptor/prognostic breast marker PR Marker of normal/malignant prostate PS Marker of normal/malignant prostate PSP Marker of parthyroid tumours PTH Marker of ovarian malignancy R1(inhibin) Marker of primary renal carcinoma RCC Nerve sheath, neural marker S100 ssessment Manager: LD1 Page 19 of 29

20 HUMN ODY TISSUE ND FLUIDS (cont d) Tissue and cell blocks (cont d) Histopathology identification of (cont d): utomated Immunohistochemical staining, Dako PTLink/utostainer using the following monoclonal, polyclonal and cocktail antibodies: Marker of serotonin producing tumours SEROT Pancreatic tumours SOMT Smooth muscle marker SM Smooth muscle marker SMM Marker of neural cells/neuroendocrine tumors SYNPT Tangles in lzheimers disease TU Marker of normal/malignant thyroid THYROG Pituitary hormone TSH Marker of normal throid/lung tumours TTF Tangles, plaques and inclusions in dementia UIQ brain Marker of Mallory bodies. UIQ liver Mesenchymal tumours Vimentin Wilms tumour/mesothelioma WT-1 Desmoplastic cell tumour WT-1 C19 ssessment Manager: LD1 Page 20 of 29

21 HUMN ODY TISSUE ND FLUIDS (cont d) Histopathology Tissue (reast primary) HER2 utomated Immunohistochemical staining, SOP 721: Use of Ventana enchmark Ultra for HER2 ntibody: anti-her-2/neu(45) Tissue (Renal) Identification of immune deposits in the glomerular membrane and mesangium HPSOP 717 Manual Immunocytochemical staining of renal biopsies Using antibodies Ig, IgM, IgG, C1q, C3d Histopathology Enzyme histochemistry Immuno fluorescence (IF) for identification/demonstration of: Tissue (Rectal) Presence/absence of ganglion cells and acetylcholinesterase when Hirschsprung s disease is suspected HPSOP 1605 cetylcholinesterase Tissue (Renal) Immunoglobulins and complement HPSOP 1606 Immunofluorescence staining of renal biopsies Using antibodies Ig IgG IgM C3C C1q Kappa Lambda Tissue (Skin) Immunoglobulins and complement HPSOP 1607 Sectioning & Immunofluorescence Staining of Skin/ Oral/Neuro frozen sections Using antibodies in Ig IgG IgM C3C Fibrinogen ssessment Manager: LD1 Page 21 of 29

22 HUMN ODY TISSUE ND FLUIDS (cont d) Histopathology Morphological assessment and interpretation/diagnosis: Slides prepared in house as above (reast) HPSOPMDLG1 001 Dissection and Reporting of reast Specimens Slides prepared in house as above (Gastrointestinal) HPSOPMDLG2 001 Reporting of colonic resection specimens with vascular pathology HPSOPMDLG2 003 Reporting of ileo-colic resection specimens HPSOPMDLG2 004 Reporting of small intestinal resection specimens HPSOPMDLG2 005 Reporting of Colorectal Cancer Specimens HPSOPMDLG2 006 Reporting of resections for pancreatic, ampullary and bile duct carcinoma HPSOPMDLG2 007 Reporting of stomach resection specimens HPSOPMDLG2 008 Reporting of oesophageal resection specimens HPSOPMDLG2 013 Reporting of endomucosal resection specimens (EMR) HPSOPMDL G4 016 Reporting of liver resections not transplant ssessment Manager: LD1 Page 22 of 29

23 HUMN ODY TISSUE ND FLUIDS (cont d) Histopathology Morphological assessment and interpretation/diagnosis: Slides prepared in house as above (Thoracic, skin) HPSOPMDLG3 003 Reporting of skin specimens, biopsies and resections HPSOPMDLG3 004 Reporting of lung resection specimens HPSOPMDLG3 005 Reporting of Thoricic lung biopsies and segmental excisions HPSOPMDLG3 006 Reporting of Soft Tissue tumours specimens, core biopsies, excisions, amputations Slides prepared in house as above (Head & neck) HPSOPMDLG4 001 Reporting of oral mucosal specimens for tumours HPSOPMDLG4 002 Reporting of thyroid specimens HPSOPMDLG4 005 Reporting of small mucosal specimens HPSOPMDLG4 006 Reporting of small bone specimens HPSOPMDLG4 007 Reporting of salivary gland specimens for tumours HPSOPMDLG4 008 Reporting of radical neck dissection specimens ssessment Manager: LD1 Page 23 of 29

24 HUMN ODY TISSUE ND FLUIDS (cont d) Histopathology Morphological assessment and interpretation/diagnosis (cont d): Slides prepared in house as above (Head & neck) (cont d) HPSOPMDLG4 009 Reporting of maxillectomy specimens for tumours HPSOPMDLG4 010 Reporting of laryngectomy specimens for tumours Slides prepared in house as above (Neuropathology) HPSOPMDLG6 002 Reporting of Globes HPSOPMDLG6 003 Reporting the examination of orbital exenterations HPSOPMDLG6 004 Reporting of wedge excisions - eyelid lesions HPSOPMDLG6 005 Reporting of Temporal rtery Specimens HPSOPMDLG6 006 Reporting of Trabeculectomy Specimens HPSOPMDLG6 007 Reporting a penetrating keratoplasty (Cornea) Slides prepared in house as above (Urology) HPSOPMDLUr 001 Prostatic biopsies, transurethral or open prosatic resections and radical prostatectomies HPSOPMDLUr 002 dult renal parenchymal tumours ssessment Manager: LD1 Page 24 of 29

25 HUMN ODY TISSUE ND FLUIDS (cont d) Histopathology Morphological assessment and interpretation/diagnosis (cont d): Slides prepared in house as above (Urology) HPSOPMDLUr 003 Penile biopsies and resections HPSOPMDLUr 004 Testicular biopsies and resections, retroperitoneal lymph node dissections HPSOPMDLUr 005 Urinary bladder, ureteric, renal pelvic and urethral specimen Slides prepared in house as above (Skin) HPSOP 1416 Screening of skin biopsies by Immunofluorescence Tissue Muscle / Nerve Histopathology Frozen section preparation (Microm Cryostat): HPSOP 1306 Cryostat Section Preparation for Histochemistry Sample preparation techniques: HPSOP 1328 Tissue Quenching for Histochemistry HPSOP1323 Specimen Handling for Muscle, Nerve, Rectal, Skin and Conjunctival iopsies Tissue processing (paraffin muscle blocks): HPSOP 1357 Processing Tissue using the Leica TP1020 Tissue Processors ssessment Manager: LD1 Page 25 of 29

26 HUMN ODY TISSUE ND FLUIDS (cont d) Tissue (cont d) Muscle / Nerve Histopathology (cont d) Identification of basophilic and eosinophilic structures HPSOP 1309 Haematoxylin and Eosin Staining Method (cryostat sections) Muscle / Nerve Histopathology - Identification of: Manual special staining techniques: Glycogen HPSOP 1317 Periodic cid Schiff (PS) and Diastase-PS Staining Method for Cryostat Sections Mitochondria and their distribution HPSOP 1308 Gomori Trichrome HPSOP 1324 Succinate Dehydrogenase Demonstration Muscle / Nerve Histopathology - Identification of normal /abnormal muscle fibre elements HP SOP 1372 Immunocytochemical Sarcolemmal Staining Method (Dako Real) Using the following monoclonal, polyclonal and antibodies: Tissue (Muscle) Dystrophin 1 Dystrophin 2 Dystrophin 3 lpha Sarcoglycan etasarcoglycan Gamma Sarcoglycan Delta Sarcoglycan Emerin ssessment Manager: LD1 Page 26 of 29

27 HUMN ODY TISSUE ND FLUIDS (cont d) Muscle / Nerve Histopathology - Identification of normal /abnormal muscle fibre elements (cont d) HP SOP 1372 Immunocytochemical Sarcolemmal Staining Method (Dako Real) Using the following monoclonal, polyclonal and antibodies (cont d): Tissue (Muscle) Dysferlin Merosin Spectrin Identification of nucleated cells MHC-1 (HL-C) Tissue (Surgical and MH muscle) Muscle / Nerve Histopathology - identification /demonstration of: Enzyme histochemical staining HPSOP 1419 Specialist Histopathology Staining Panels cid Phosphatase HPSOP 1304 cid Phosphatase Naphthol S-I Phosphate Fibres with abnormal COX activity Myoadenylate Deaminase denosine triphosphatase NDH PFK and ldolase activity Myophosphorylase in muscle HPSOP 1383 Combined Demonstration of Cytochrome Oxidase & Succinate Dehydrogenase HPSOP 1307 Cytochrome Oxidase Demonstration HPSOP 1312 Myoadenylate Deaminase Demonstration HPSOP 1313 Myosin TPase Staining Method HPSOP 1314 NDH - Tetrazolium Reductase Demonstration HPSOP 1319 Phosphofructokinase and ldolase Demonstration HPSOP 1364 Phosphorylase Demonstration ssessment Manager: LD1 Page 27 of 29

28 HUMN ODY TISSUE ND FLUIDS (cont d) Tissue (Muscle) Muscle / Nerve Histopathology - identification /demonstration of: Manual tinctorial Stain techniques for: Presence and distribution of lipid in muscle HPSOP 1321 P-Phenylene diamine Staining Method HPSOP 1365 Sudan lack Staining Method Myelin in nerves HP SOP 1316 Palade Fixation/Staining Method Internode length, fibre diameter and myelin degeneration HPSOP 1327 Teased Nerve Procedure Slides prepared in house as above Muscle / Nerve Histopathology - Morphological assessment and interpretation/diagnosis HPSOPMDL G6 011 Reporting of muscle biopsy specimen examination and handling HPSOPMDL G6 012 Reporting of peripheral nerve biopsy specimen examination ssessment Manager: LD1 Page 28 of 29

29 HUMN ODY FLUIDS ndrology examination activities for the purposes of clinical diagnosis: ll techniques carried out manually according to WHO (2010) guidelines using Phase contrast microscopy: Semen Infertility testing: Volume ppearance Viscosity ph Motility gglutination Sperm density Sperm motility Morphology HPSOP 850 Infertility testing HPSOP 851 Sperm density HPSOP 852 Motility testing HPSOP 861 Sperm morphology D Post vasectomy testing: Sperm presence/absence HPSOP 871 Post vasectomy testing D Haematoxylin & Eosin staining for identification of basophilic and eosinophilic structures to demonstrate morphology of cells and spermatozoa Sample staining: HPSOP 868 H&E staining D END ssessment Manager: LD1 Page 29 of 29