Sample validation data
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1 Bioware and Bioware Ultra oncology cell lines Sample validation data Animal models and cell injection number A549-luc-C8 (Cat. No ) Model Subcutaneous Intravenous Male nu/nu Male nu/nu (Charles River) Thigh Thigh Male nu/nu 5 x10 6, 1 x x10 6, 1 x x10 6 5x10 6 5x10 6 5x10 6 Tumor Take 100% 100% 100% 100% Met Met Sites 20-60% ex vivo only (1/5) Intercostal muscle (3/5) 13% in lungs 100% in lungs 100% in lungs (1/8) (13/13) Liver (4/13) Intercostal muscle (3/13) Pleural (8/8) 2/6 in vivo; 5/6 ex vivo 80% in lungs ex vivo (5/6) ex vivo 0/8 in vivo; 5/8 ex vivo 60% in lungs ex vivo (5/8) ex vivo Initial Met End point (ex Week 7 Day 0 Day vivo Study Length only) 7 weeks 10 weeks 4-7 weeks 7 weeks 14 weeks 7 weeks
2 B16-F10-luc-G5 (Cat. No ) Model Subcutaneous Intravenous Male Nu/nu Male Nu/nu Male C57BL/6 Albino (Jackson Laboratory) nea r thigh 1-5x10 5 5x10 5 5x10 5 None > 90% > 90% Sites No Mets (90-100%) Ribs, Fat (80%) Liver (30%) Lymph nodes (10-30 %) - mesentery - axillary/ brachial LN - inguinal LN - cervical LN Others (< 10%) - urogenital system - kidneys - panc reas - stomach (80-90%) Liver (15-30%) Fat (15-30%) Kidneys (15-30 %) Pancreas (15-30 %) Initial N/A Week 1 Week 1 Study Length 2-3 weeks 2-3 weeks 2-3 weeks Notes N/A At < 5x10 5 cells, see less frequency of lung metastases at end point At < 5x10 5 cells, see less frequency of lung metastases at end point 2/12
3 HeLa-luc (Cat. No ) Model Subcutaneous Intravenous near thigh 1x10 6 left flank 5x10 6 right flank Female Nu/nu near thigh Female Nu/nu (Charles River) 1x10 6 left flank 5x10 6 right flank 5x10 6 5x10 6 Tumor Take 100% (5/5) 100% (5/5) 100% (5/5) 0% (0/5) 100% 100% 100% N/A Sites (Ex Vivo only unless otherwise noted) (100%) - in vivo: 40% (wk4) Liver (60%) Ovaries (40%) Ribs (60%) (80%) - in vivo: 60% (wk4) Liver (40%) Ovaries (40%) Ribs (100%) (100%) Liver (80%) Ovaries (60%) Stomach (60%) Intestines (40%) Spleen (20%) Kidneys (60%) Pancreas (60%) Salivary Glands (40%) Jaw (40%) Spinal Cord (80%) Legs (80%) Ribs (60%) Initial Week 3-4 Week 3-4 Week 2 N/A Study Length 4-5 weeks 4-5 weeks 4-7 weeks 7 weeks N/A 3/12
4 HT-29-luc-D6 (Cat. No ) Models Subcutaneous Metastasis Mo del Mouse St rain Male SCID- bg Charles R iver (C.B -17/lcrCrl-sc idbgbr ) near thigh 1 x and 3 x 10 (two sites ) Male SCID- bg Charles R iver (C.B -17/lcrCrl-sc idbgbr ) near thigh 3 x10 6 (one site) Tumor Take 100% 100% Met In vivo: % Ex vivo: % In vivo: % Ex vivo: 33 78% M et Sites 0-80% Lymph Nodes (axillary, brachial) 22-33% Lymph Nodes (axillary, brachial) 40-63% Lu ngs 22-33% Initial Met Weeks 2-6 Weeks 2-5 Study Length 7-8 weeks 7-8 weeks 4/12
5 LL/2-luc-M38 (Cat. No ) Model Subcutaneous Intravenous Mouse Strain Cell Number Tumor Take Sites Initial Study Length near thigh Female C57BL/6-Albino (Jackson Labs) near thigh Female C57BL/6 (Charles River) near thigh Female C57BL/6-Albino (Jackson Labs) Female C57BL/6 1x10 6 1x10 6 1x10 6 1x10 6 1x10 6 2x10 6 1x10 6 2x % 100% 100% % % % % 33% 56% % % % (100%) (in vivo) Ribs (33-60%) (ex-vivo) Heart (11-33%) (ex-vivo) (33%) (in vivo) Ribs (60%) (ex vivo) Heart (33%) (ex vivo) (78%) (ex vivo) 11% spleen (ex vivo) (90-100%) (in vivo) Ribs (87-100%) (ex vivo) Heart (73-78%) (ex vivo) Jaw (0-40%) (in vivo) Abdominal area (0-56%) (in vivo) Lower Spine Area (0-33%) (ex vivo) Leg/pelvis (0-33%) (ex vivo) Salivary Gland (0-11%) (ex vivo) Head Region (0-11%) (ex vivo) (90-100%) (in vivo) Ribs(70-100%) (ex vivo) Heart (10-70%) (ex vivo) Lower Spine Area (0-20%) (in vivo) Abdominal (0-20%) (ex vivo) Jaw (10%) (in vivo) Leg (0-10%) (ex vivo) Day 7 Day 21 Day 17 Day 7 Day 7 Day 11 (90%) (in vivo) Ribs (90%) (ex vivo) Heart (10%) (ex vivo) Abdominal (10%) (ex vivo) 4 weeks 4 weeks 4 weeks 3-4 weeks 3-4 weeks 3-4 weeks 5/12
6 LNCaP-luc-M6 (Cat. No ) Model Subcutaneous Orthotopic (Charles River and Harlan) near thigh Prostate 3x10 6 in 50 % Matrigel 1x10 6 Tumor Take Variable (0-100 %) 50-90% In vivo: 90 % Ex vivo: 100 % In vivo: % Ex vivo: % Sites : 10 /10 Ribs w/ associated tissue: 7 /10 Peritoneum: 5/10 Inguinal LN: 1/10 Preputial Gland : 4/10 Pancreas: 1/10 : 12 /13 Ribs w/ associated tissue: 11 /13 Male sex organs: 3/13 Intestine: 2/13 Preputial gland : 2/13 Initial Weeks 7-11 Weeks 7-10 Study Length 7-16 weeks 8 16 weeks * Nude mice are NOT recommended for LNCaP-luc-M6 tumor growth 6/12
7 LoVo-6-luc-1 (Cat. No ) Model Subcutaneous Intraperitoneal Ceacum (Charles River: CB- 17/lcrCRl-scidbgBR) (Charles River: C.B- 17/lcrCrl-scid-bgBR) (Charles River: C.B- 17/lcrCrl- scid-bgbr) near thigh Intraperitoneal cavity Serosa of ceacum 1x10 6 and 3x10 6 (two sites) 2x10 6 cells 4x10 6 cells Tumor Take 100% for 3x10*6 cells 80% for 1x10*6 cells 100% 86% Met 0% 100% 86% Study Length 6 weeks 7 weeks 9 weeks 7/12
8 MDA-MB-231-luc-D3H1 (Cat. No ) Model Intravenous Intracardiac Orthotopic Orthotopic Female nude b eige Charles River NIH-III (N:NIH-bg-nu-xidBR) Female nude Harlan (Hsd: Athymic Nude-nu) Female nude Harlan (Hsd: Athymic Nude-nu) Female nude b eige Charles River NIH-III (N:NIH-bg-nu-xidBR) Heart-left ventricle Mammary fat pad Mammary fat pad 2x10 6 1x10 5 2x10 6 2x % 80% % 70-80% Sites (70-80%) Rib/tissue (30 %) Rib/tissue (80 %) (40%) Lymph node (40%) (brachial/axillary) Scapula (20%) Lymph node (90-100%) (brachial/axillary) (50-60%) Lymph node (70%) Rib/tissue (100 %) Initial Week 4-5 Week 5 Week 6-7 Week 9-12 Study Length 11 weeks 10 weeks we eks 13 weeks 8/12
9 MDA-MB-231-luc-D3H2LN (Cat. No ) Model Orthotopic Intracardiac Female nude Harlan (Hsd: Athymic Nudenu) Female nude beige Charles River NIH-III (N:NIH-bg-nu-xidBR) Female nude Harlan (Hsd: Athymic Nudenu) Female nude beige Charles River NIH-III (N:NIH-bg-nu-xidBR) Mammary gland Mammary gland Heart-left ventricle Heart-left ventricle Heart-left ventricle 2x10 6 2x10 6 1x10 5 1x10 5 1x10 5 Tumor Take 89% (8/9) 67% (6/9) 100% (8/8) 100% (3/3) 100% (3/3) Pilot Study Pilot Study 100% 100% 100% 100% 100% Sites Lymph Node (100%) (brachial/axillary) (50%) Lymph node (100%) Rib/Tissue (67%) Spine (88%) Skull (75%) Scapula (75%) Femur/Tibia (63%) Clavicle (50%) Rib/tissue (100%) Brain (88%) (88%) Lymph Node (63%) (brachial/axillary) Spine (67%) Skull (33%) Scapula (33%) Femur/Tibia (33%) Clavicle (33%) Rib/tissue (33%) Brain (33%) (67%) Lymph Node (0%) (brachial/axillary) Spine (67%) Skull (33%) Scapula (33%) Femur/Tibia (33%) Clavicle (0%) Rib/tissue (67%) Brain (33%) (67%) Lymph Node (0%) (brachial/axillary) Initial Week 3-4 Week 4-6 Week 3-4 Week 3-4 Week 3-4 Study Length 5 weeks 9 weeks 4 weeks 6 weeks 9 weeks 9/12
10 MCF-7-luc-F5 (Cat. No ) Models Orthotopic Model Female nu/nu Charles River (Crl:NU/NU-nuBR) Female nude-beige Harlan (Beige-nude XID nu/nu) Female nude-beige Harlan (Beige-nude XID nu/nu) Abdominal mammary fat pad Abdominal mammary fat pad Abdominal mammary fat pad 2 x x x10 6 Estrogen 17ß-Estradiol, 0.72 mg/pellet, 60 day release 17ß-Estradiol, 0.36 mg/pellet, 60 day release 17ß-Estradiol, 0.36 mg/pellet, 60 day release Tumor Take 64% (18/28) 97% (33/34) 92% (24/26) Met In vivo: 0% Ex vivo: 0% In vivo: 86% (6/7) Ex vivo: 100% (7/7) In vivo: 71% (5/7) Ex vivo: 86% (6/7) Met Sites n/a : 100% Forelimbs: 57% Lymph nodes: 14% (axillary / brachial) : 86% Forelimbs: 0% Lymph nodes: 0% (axillary / brachial) Initial Met n/a Week 6 Week 5 Study Length 5-9 weeks 8 weeks 14 weeks 10/12
11 PC-3M-luc-C6 (Cat. No ) Model Subcutaneous Intravenous Intracar diac Orthotopic Mouse Strain Cell Number Tumor Take Sites Initial Study Length Male Nu/nu (Charles Dorsal region near Male SCIDbg (Charles Dorsal region near thigh Male Nu/nu Male Nu/nu Heart (left ventricle) Male Nu/nu (Charles River) Prostate 3x x10 6 5x10 6 5x10 6 3x10 6 5x % 100% N/A N/A N/A 100% N/A > 90% 50% 100% > 90% N/A N/A N/A (>90%) Inguinal LN (>90%) Leg (~50%) Liver (~50%) Other LNs (~50%) - Axillary - Brachial Kidney (<20%) Spleen (<20%) Pancreas (<20%) Week 3 (3x10 6 ) Week 5 (1x10 6 ) (50%) Heart (17%) In Vivo Head region: 4/12 mice Thorax: 8/12 mice Abdomen: 9/12 mice Leg region: 3/12 mice Ex Vivo Jaws (25%) Skull, Salivary (17%) (100%) Ribs/ pleural (58%) Heart, scapula (17%) Adrenal gland (42%) Pancreas, liver (25%) Vas deferens, spine (17%) Leg (17%) In Vivo Head region: 5/6 mice Thorax: 6/6 mice Leg region: 5/6 mice Ex Vivo Dental pulp (50%) Periodontal incisor (33%) Cervical LN (17%) Pleural surface (83%) Muscle to spine (33%) Rib (17%) Mediastinum (17%) Week 3 Week 3 Week 1 N/A 5-7 weeks 6-8 weeks 6-8 weeks 6-8 weeks Tibia 3-4 weeks (75%) Femur (25%) 4-6 weeks N/A 11/12
12 SKOV3-luc-D3 (Cat. No ) Model Subcutaneous Intraperitoneal Female Nu/nu Female Nu/nu near thigh 1x10 6 left flank 5x10 6 right flank near thigh 6 1x10 left flank 5x10 6 right flank 6 5x10 5x10 6 Tumor Take 100% (3/3) 80% (4/5) 100% (3/3) 80% (4/5) 9% (1/11) 0% 0% Sites Initial N/A N/A N/A Week 13 N/A N/A N/A Study Length weeks weeks weeks weeks PerkinElmer, Inc. 940 Winter Street Waltham, MA USA P: (800) or (+1) For a complete listing of our global offices, visit Copyright 2013, PerkinElmer, Inc. All rights reserved. PerkinElmer is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners. 12/12
Species Tumor Type Comment for in vivo work Lead Time for in vivo studies [weeks] MB-49-luc-2 Mouse urinary bladder carcinoma C57BL/6 2
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