OIE Collaborating Centres Reports Activities
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1 OIE Collaborating Centres Reports Activities Activities in 2015 This report has been submitted : :17:34 Title of collaborating centre: Validation, QA Europe Address of Collaborating Centre: CODA/CERVA/VAR Department of Virology Section Epizootic Diseases Groeselenberg 99 B-1180 Ukkel BELGIUM Tel.: Fax: address: krdec@coda-cerva.be Website: Name of Director of Institute (Responsible Official): Dr Pierre Kerkhofs Name (including Title and Position) of Head of the Collaborating Centre (formally OIE Contact Point): Dr Kris De Clercq, Head of Unit Name of writer: Kris De Clercq OIE Collaborating Centres Reports Activities,
2 ToR: To provide services to the OIE, in particular within the region, in the designated specialty, in support of the implementation of OIE policies and, where required, seek for collaboration with OIE Reference Laboratories ToR: To identify and maintain existing expertise, in particular within its region 1. Activities as a centre of research, expertise, standardisation and dissemination of techniques within the remit of the mandate given by the OIE Training, capacity building Title of activity Scope OIE Twinning between the OIE CC CODA-CERVA Belgium and NVRI, Vom, Nigeria. This twinning project aims to build the capacity of the FMD Unit at the NVRI for the improvement of the diagnosis of and control of foot-and-mouth disease virus (FMDV) in Nigeria and in order to prepare the Candidate laboratory to evolve later on towards the level of OIE Reference Laboratory. Diagnosis, biotechnology and laboratory Title of activity Scope Evaluation and quality control of the performance parameters of a Lateral Flow Test device for the detection of Bluetongue virus-specific antibodies. A commercial rapid immuno-chromatographic method or Lateral Flow Test (LFT) device, for the detection of BT virusspecific antibodies in animal serum, was evaluated. It can be concluded that the BT LFT device is a rapid and sensitive first-line serological test that can be used in the field. This was done in full collaboration between the OIE CC Coda- Cerva, Belgium; the OIE Ref Centre Pirbright Laboratory, UK; the OIE Ref Centre Anses, France; the OIE CC FLI, Germany; SVA, Uppsala, Sweden; UGent, Merelbeke, Belgium. Vaccines Title of activity Scope Quality control of live attenuated or inactivated lumpy skin disease or sheepox vaccines. The scope of this activity is to control the quality of commercially available or newly developed live attenuated or inactivated lumpy skin disease or sheepox vaccines against an LSDV infection in cattle. The first 5 vaccines were tested in The aim of the study is to evaluate whether the LSD attenuated vaccines are able to provide protection in an efficient and safe (side effects) way and to check whether inactivated vaccines could be an interesting alternative. ToR : To propose or develop methods and procedures that facilitate harmonisation of international standards and guidelines applicable to the designated specialty 2. Proposal or development of any procedure that will facilitate harmonisation of international regulations applicable to the surveillance and control of animal diseases, food safety or animal welfare 2 OIE Collaborating Centres Reports Activities, 2015
3 Proposal title Scope/Content Applicable area Virus strains used in the laboratory for serological (virus neutralisation, ELISAs) or virological (virus isolation, antigen Quality control of virus strains use in diagnostic assays and for vaccine evaluation. ELISA) assays as antigen or as control are checked on a yearly basis by sequencing to evaluate possible deviation after Surveillance and control of animal diseases Food safety Animal welfare cultivation or passages from the original strains or from the reference strains. Reference strains are obtained from the OIE Ref Centre at Pirbright, UK. ToR: To establish and maintain a network with other OIE Collaborating Centres designated for the same specialty, and should the need arise, with Collaborating Centres in other disciplines ToR: To carry out and/or coordinate scientific and technical studies in collaboration with other centres, laboratories or organisations 3. Did your Collaborating Centre maintain a network with other OIE Collaborating Centres (CC), Reference Laboratories (RL), or organisations designated for the same specialty, to coordinate scientific and technical studies? Name of OIE CC/RL/other organisation(s) Location Region of networking Centre Purpose OIE Collaborating Centres Reports Activities,
4 - OIE/FAO FMD Reference Laboratory Network - Vaccine matching The OIE FMD Reference Laboratory The Pirbright Laboratory UK UK Africa Americas Asia and Pacific Europe Middle East - International proficiency tests - Post vaccination monitoring - Virus and sequence exchange - Obtaining viral reference strains 4. Did your Collaborating Centre maintain a network with other OIE Collaborating Centres, Reference laboratories, or organisations in other disciplines, to coordinate scientific and technical studies? Name of OIE CC/RL/other organisation(s) Location Region of networking Centre Purpose The OIE Bluetongue Reference Laboratory Pirbright Laboratory UK UK Africa Americas Asia and Pacific Europe Middle East Diagnostic assay and vaccine quality control for bluetongue virus ToR: To place expert consultants at the disposal of the OIE. 5. Did your Collaborating Centre place expert consultants at the disposal of the OIE? Name of expert Kind of consultancy Subject Kris De Clercq Vice President SCAD OIE mission: Endorsement FMD control programme. India, 10-17/01/2015 ToR: To provide, within the designated specialty, scientific and technical training to personnel from OIE Member Countries 6. Did your Collaborating Centre provide scientific and technical training, within the remit of the mandate given by the OIE, to personnel from OIE Member Countries? 4 OIE Collaborating Centres Reports Activities, 2015
5 a) Technical visits: 2 b) Seminars: 1 c) Hands-on training courses: 13 d) Internships (>1 month): 2 Validation, QA Europe - Centre d'etudes et de Recherches Vétér Type of technical training provided (a, b, c or d) Content Country of origin of the expert(s) provided with training No. participants from the corresponding country a LSD diagnosis as differential diagnosis of FMD, real-time RT- PCR,sequencing, quality control, accreditation The Netherlands 1 ToR: To organise and participate in scientific meetings and other activities on behalf of the OIE 7. Did your Collaborating Centre organise or participate in the organisation of scientific meetings on behalf of the OIE? National/International Title of event Co-organiser Date (mm/yy) Location No. Participants International OIE/FAO FMD Reference Laboratories Network meeting OIE Ref Centre FMD Pirbright UK 11/2015 Ukkel Belgium 39 ToR: To collect, process, analyse, publish and disseminate data and information relevant to the designated specialty 8. Publication and dissemination of any information within the remit of the mandate given by the OIE that may be useful to Member Countries of the OIE a) Articles published in peer-reviewed journals: 7 Hanon J.B., Vandenberge V., Deruelle M., De Leeuw I., De Clercq K., Van Borm S., Koenen F., Liu L., Hoffmann B., Batten C.A., Zientara S., Breard E., Van der Stede Y. (2015). Inter-laboratory evaluation of the performance parameters of a Lateral Flow Test device for the detection of Bluetongue virus-specific antibodies. J Virol Methods. 228: Breard E., Belbis G., Viarouge C., Nomikou K., Haegeman A., De Clercq K., Hudelet P., Hamers C., Moreau F., Lilin T., Durand B., Mertens P., Vitour D., Sailleau C., Zientara S. (2015). Evaluation of adaptive immune responses and heterologous protection induced by inactivated bluetongue virus vaccines. Vaccine 33(4): Haegeman A., Zro K., Sammin D., Vandenbussche F., Ennaji M.M., De Clercq K. (2015). Investigation of a Possible Link Between Vaccination and the 2010 Sheep Pox Epizootic in Morocco. Transbound Emerg Dis.. doi: /tbed OIE Collaborating Centres Reports Activities,
6 De Vleeschauwer A., Qiu Y., Van Reeth K. (2015). Vaccination-challenge studies with a Port Chalmers/73 (H3N2)- based swine influenza virus vaccine: reflections on vaccine strain updates and on the vaccine potency test. Vaccine 33(20): van der Linden L., Vives-Adrián L., Selisko B., Ferrer-Orta C., Liu X., Lanke K., Ulferts R., De Palma A.M., Tanchis F., Goris N., Lefebvre D., De Clercq K., Leyssen P., Lacroix C., Pürstinger G., Coutard B., Canard B., Boehr D.D., Arnold J.J., Cameron C.E., Verdaguer N., Neyts J., van Kuppeveld F.J. (2015). The RNA Template Channel of the RNA- Dependent RNA Polymerase as a Target for Development of Antiviral Therapy of Multiple Genera within a Virus Family. PLoS Pathog. 11(3):e De Leeuw I., Garigliany M., Bertels G., Willems T., Desmecht D., De Clercq K. (2015). Bluetongue virus RNA detection by real-time rt-pcr in post-vaccination samples from cattle. Transbound Emerg Dis. 62(2): Vandenbussche F, Sailleau C, Rosseel T, Desprat A, Viarouge C, Richardson J, Eschbaumer M, Hoffmann B, De Clercq K, Bréard E, Zientara S. (2015). Full-Genome Sequencing of Four Bluetongue Virus Serotype 11 Viruses. Transbound Emerg Dis. 62(5): b) International conferences: 8 Beer M., De Clercq K., Lefebvre D., De Vleeschauwer A., Schwartz Cornil, I. Valarcher J.-F., Hägglund S., Haas B., Hudelet P., Richardso, J., Klonjkowski B., Blaise Boisseau S., Bakkali Kassimi L., Zientara S. ANIHWA PROJECT "TRANSCRIPTOVAC": Host response gene signatures associated with FMDV infection, vaccination and persistence. Proceedings of the Xth International Congress of Veterinary Virology ESVV2015 and 9th Annual Meeting of EPIZONE, p Haegeman A., Zro K., vandenbussche F., Sammin D., Ennaji M.M., De Vleeschauwer A., De Clercq K. Investigation of a possible link between vaccination and the 2010 Sheep pox epizootic in Morocco. Proceedings of the Xth International Congress of Veterinary Virology ESVV2015 and 9th Annual Meeting of EPIZONE, p Martinelle L., Haegeman A., Lignereux L., Chaber A-L., Dal Pozzo F., De Leeuw I., De Clercq K., Saegerman C. Orbivirus screening on dried blood spots from captive oryx in United Arab Emirates stresses the importance of pre-import measures. Proceedings of the Xth International Congress of Veterinary Virology ESVV2015 and 9th Annual Meeting of EPIZONE, p De Leeuw I., Garigliany M., Bertels G., Willems T., Desmecht D., De Clercq K. Bluetongue virus RNA detection by real-time RT-PCR in post-vaccination samples from cattle. Scientific report CODA-CERVA , p Haegeman A., Zro K., Sammin D., Vandenbussche F., Ennaji M.M., De Clercq K. Investigation of a Possible Link Between Vaccination and the 2010 Sheep Pox Epizootic in Morocco. Scientific report CODA-CERVA , p Lefebvre D., De Vleeschauwer A., Goris N., Van Borm S., Chimirri A., Monforte A.M., Valdazo-Gonzalez B., King D.P., Neyts J., De Clercq K. A thiazepino[4,5-a]benzimidazole derivative hampers the RNA replication of Eurasian serotypes of foot-and-mouth disease virus. Scientific report CODA-CERVA , p Vandenbussche F., Sailleau C., Rosseel T., Desprat A., Viarouge C., Richardson J., Eschbaumer M., Hoffmann B., De Clercq K., Bréard E., Zientara S. Full-Genome Sequencing of Four Bluetongue Virus Serotype 11 Viruses. Scientific report CODA-CERVA , p Rosseel T., Pardon B., De Clercq K., Ozhelvaci O., Van Borm S. False-positive results in metagenomic virus discovery: a strong case for follow-up diagnosis. Scientific report CODA-CERVA , p c) National conferences: 1 De Clercq K. (2015). The International dimention of the Belgium Reference Laboratory for Vesicular Diseases. Network of Veterinatians in research and Industry, Brussels, 13 November 2015 d) Other (Provide website address or link to appropriate information): 0 6 OIE Collaborating Centres Reports Activities, 2015
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