OIE Reference Laboratory Reports Activities
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1 OIE Reference Laboratory Reports Activities Activities in 2015 This report has been submitted : :10:43 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Marek s disease Address of laboratory: USDA-Agricultural Research Service Avian Disease and Oncology Laboratory 4279 E Mount Hope Road East Lansing Michigan UNITED STATES OF AMERICA Tel.: Fax: address: John.Dunn@ARS.USDA.GOV Website: Name (including Title) of Head of Laboratory (Responsible Official): Vacant Name (including Title and Position) of OIE Reference Expert: John R. Dunn (Research Veterinary Medical Officer) Which of the following defines your laboratory? Check all that apply: Governmental OIE Reference Laboratory Reports Activities,
2 ToR 1: To use, promote and disseminate diagnostic methods validated according to OIE Standards 1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as disease diagnosis, screening of animals for export, surveillance, etc.? (t for quality control, proficiency testing or staff training) Diagnostic Test Indicated in OIE Manual (/) Total number of test performed last year Indirect diagnostic tests Nationally Internationally IFA 15 0 Direct diagnostic tests Nationally Internationally Cell culture PCR ToR 2: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease. 2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE? 3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents to OIE Member Countries? 2 OIE Reference Laboratory Reports Activities, 2015
3 Type of reagent available Related diagnostic test Produced/ provide Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg). of recipient OIE Member Countries Region of recipients Monoclonal antibodies/hybridoma cells IHC, FA Produced 6 ml 12 ml 4 Africa Americ as Asia and Pacific Europe Middle East Prototype strains of MDV Vaccine development, challenge experiments Produced 4 ml 1 Africa Americ as Asia and Pacific Europe Middle East 4. Did your laboratory produce vaccines? 5. Did your laboratory supply vaccines to OIE Member Countries? Vaccine name Amount supplied nationally (ml, mg) (including for own use) Amount supplied to other countries (ml, mg) Name of recipient OIE Member Countries HVT, rhvt, SB1, Rispens 75 ml 0 UNITED STATES OF AMERICA ToR 3: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases 6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease? 7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? OIE Reference Laboratory Reports Activities,
4 ToR 4: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries 8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? Name of OIE Member Country seeking assistance Date (month). samples received for provision of diagnostic support. samples received for provision of confirmatory diagnoses ITALY January 0 17 EGYPT September 0 3 SENEGAL February Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? Name of the OIE Member Country receiving a technical consultancy Purpose How the advice was provided UNITED STATES OF AMERICA Multiple consultations regarding diagnosis of avian tumors By UNITED KINGDOM For questions about MD diagnosis By ITALY Procedures for MDV titration and vaccine questions By ToR 5: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations 10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? Title of the study Duration Purpose of the study Partners (Institutions) The role of host genetic resistance and vaccination on transmission of Marek's disease virus in poultry 5 years To determine genetic component of infectivity Roslin Institute 4 OIE Reference Laboratory Reports Activities, 2015
5 ToR 6: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases 11. Did your Laboratory collect epizootiological data relevant to international disease control? 12. Did your laboratory disseminate epizootiological data that had been processed and analysed? 13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: Kulkarni, G., Zhang, H Evaluation of reproductive characteristics of 21 highly inbred lines of White Leghorns divergently selected for or segregating in tumor resistance. Open Journal of Animal Sciences. 5: doi: /ojas Hunt, H.D., Dunn, J.R The influence of major histocompatibility complex and vaccination with turkey herpesvirus on Marek's disease virus evolution. Avian Diseases. 59(1): Cao, W., Mays, J.K., Kulkarni, G., Dunn, J.R., Fulton, R.M., Fadly, A.M Further observations on serotype 2 Marek's disease virus-induced enhancement of spontaneous avian leukosis virus-like bursal lymphomas in ALVA6 transgenic chickens. Avian Pathology. 44(1): DOI: / Zhang, X., Misztal, I., Heidaritabar, M., Bastiaansen, J.W., Hawken, R.R., Okimoto, R., Sapp, R.L., Cheng, H.H., Muir, W.M Prior genetic architecture impacting genomic regions under selection: An example using genomic selection in two poultry breeds. Livestock Science. 171(2015):1-11. doi: /j.livsci Gimeno, I.M., Cortes, A.L., Faiz, N.M., Hernandez-Ortiz, B.A., Guy, J.S., Hunt, H.D., Silva, R.F Evaluation of the Protection Efficacy of a Serotype 1 Marek's Disease Virus-Vectored Bivalent Vaccine Against Infectious Laryngotracheitis and Marek's Disease. Avian Diseases. 59(2): Hildebrandt, E., Dunn, J.R., Cheng, H.H Addition of a UL5 helicase-primase subunit point mutation eliminates bursal-thymic atrophy of Marek s disease virus delta-meq recombinant virus but reduces vaccinal protection. Avian Pathology. Volume 44(4): Heidari, M., Fitzgerald, S.D., Zhang, H Immune Responses in Cecal Tonsils of Marek's Disease Virus Infected Chickens. Avian Diseases. 59(2): Chen, W., Yang, L., Li, H., Chang, S., Shu, D., Zhang, H., Chen, F., Xie, Q Intronic deletions of tva receptor gene decrease the susceptibility to infection by subgroup A avian sarcoma and leukosis virus subgroup A. Scientific Reports. 5:9900. doi: /srep Preeyanon, L., Brown, C., Cheng, H.H Transcriptome variation in response to Marek s disease virus acute infection. Cytogenetics and Genome Research. 145: Hildebrandt, E., Dunn, J.R., Cheng, H.H Characterizing in vivo stability and potential interactions of a UL5 helicase-primase mutation previously shown to reduce virulence and in vivo replication of Marek's disease virus. Virus Research. 203: Mitra, A., Luo, J., He, Y., Gu, Y., Zhang, H., Zhao, K., Cui, K., Song, J Histone modifications induced by MDV infection at early cytolytic and latency phases. Biomed Central (BMC) Genomics. 16:311. doi: /s Kennedy, D.A., Dunn, J.R., Dunn, P.A., Read, A.F An observational study of the temporal and spatial patterns of Marek's-disease-associated leukosis condemnation of young chickens in the United States of America. Preventive veterinary medicine, 120(3-4): p Cheng, H.H., Perumbakkam, S., Black Pyrkosz, A.A., Dunn, J.R., Muir, W.M Fine mapping of QTL and genomic prediction using allele-specific expression SNPs demonstrates that the complex trait of genetic resistance to Marek s disease is predominantly determined by transcriptional regulation. Biomed Central (BMC) Genomics. DOI: /s Yan, Y., Yang, N., Cheng, H.H., Song, J., Qu, L Genome-wide identification of copy number variations between two chicken lines that differ in genetic resistance to Marek s disease. Biomed Central (BMC) Genomics. 16(843):1-10. b) International conferences: 2 OIE Reference Laboratory Reports Activities,
6 c) National conferences: 6 d) Other: (Provide website address or link to appropriate information) 14 ToR 7: To provide scientific and technical training for personnel from OIE Member Countries To recommend the prescribed and alternative tests or vaccines as OIE Standards 14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries? a) Technical visits: 3 b) Seminars: 0 c) Hands-on training courses: 0 d) Internships (>1 month): 2 Type of technical training provided (a, b, c or d) Country of origin of the expert(s) provided with training. participants from the corresponding country a China 1 a Poland 1 a Thailand 1 d USA 2 ToR 8: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned 15. Does your laboratory have a Quality Management System certified according to an International Standard? Explain Quality Management System in adoption process or currently in place Our lab receives annual inspections from Michigan State University and USDA-APHIS for BSL-2 standards. All assays are conducted using appropriate positive and negative controls to assure quality results. 16. Is your laboratory accredited by an international accreditation body? 6 OIE Reference Laboratory Reports Activities, 2015
7 17. Does your laboratory maintain a biorisk management system for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2014, Chapter 1.1.3a) ToR 9: To organise and participate in scientific meetings on behalf of the OIE 18. Did your laboratory organise scientific meetings on behalf of the OIE? 19. Did your laboratory participate in scientific meetings on behalf of the OIE? ToR 10: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results 20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease? 21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests? 22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest? Title of the project or contract Scope Name(s) of relevant OIE Reference Laboratories Material transfer agreements for exchange of reagents Transfer of materials and referral for diagnostic support IAH, UK OIE Reference Laboratory Reports Activities,
8 ToR 11: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results 23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease? te: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: see point 1.3 ToR 12: To place expert consultants at the disposal of the OIE 24. Did your laboratory place expert consultants at the disposal of the OIE? 25. Additional comments regarding your report: 8 OIE Reference Laboratory Reports Activities, 2015
OIE Reference Laboratory Reports Activities
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