OIE Reference Laboratory Reports Activities
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1 OIE Reference Laboratory Reports Activities Activities in 2016 This report h been submitted : :23:58 Name of disee (or topic) for which you are a designated OIE Reference Laboratory: Equine influenza Address of laboratory: Lanwades Park, Kentford Suffolk CB8 7UU UNITED KINGDOM Tel.: Fax: address: debra.elton@aht.org.uk Website: Name (including Title) of Head of Laboratory (Responsible Official): Dr Mark Vaudin, Chief Executive Officer Name (including Title Position) of OIE Reference Expert: Dr Debra Elton, Head of Virology Which of the following defines your laboratory? Check all that apply: Other: Charity OIE Reference Laboratory Reports Activities,
2 ToR 1: To use, promote disseminate diagnostic methods validated according to OIE Stards 1. Did your laboratory perform diagnostic tests for the specified disee/topic for purposes such disee diagnosis, screening of animals for export, surveillance, etc.? (t for quality control, proficiency testing or staff training) Diagnostic Test Indicated in OIE Manual (/) Total number of test performed lt year Indirect diagnostic tests Nationally Internationally Haemagglutination Inhibition H3 & H SRH H3 & H7 1 0 Direct diagnostic tests Nationally Internationally qrt-pcr, M1 & NP genes Virus isolation in eggs NP ELISA 46 0 ToR 2: To develop reference material in accordance with OIE requirements, implement promote the application of OIE Stards. To store distribute to national laboratories biological reference products any other reagents used in the diagnosis control of the designated pathogens or disee. 2. Did your laboratory produce or supply imported stard reference reagents officially recognised by the OIE? 3. Did your laboratory supply stard reference reagents (non OIE-approved) /or other diagnostic reagents to OIE Member Countries? 2 OIE Reference Laboratory Reports Activities, 2016
3 Type of reagent available Related diagnostic test Produced/ provide Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg). of recipient OIE Member Countries Region of recipients Inactivated H3N8 antigen, A/equine/Richmond/07 HI Produced 0 10 ml 1 Et Inactivated H7N7 antigen, A/equine/Prague/56 HI Produced 0 10 ml 1 Et Plmid DNA containing M1 gene qrt-pcr Produced mg 1 Et Plmid DNA containing NP gene qrt-pcr Produced 0 0.1mg 1 Et Inactivated ferret sera to H3N8 strains HI Produced 0 10 ml Et OIE Reference Laboratory Reports Activities,
4 Inactivated post infection equine serum to H3N8 HI Produced ml 1 Et Inactivated post vaccination equine serum to H3N8 & H7N7 HI Produced ml 1 Et Inactivated negative control equine serum HI Produced 0 3 ml 1 Et H3N8 Florida clade 1 purified RNA qrt-pcr Produced ml 1 Et 4. Did your laboratory produce vaccines? 5. Did your laboratory supply vaccines to OIE Member Countries? ToR 3: To develop, stardise validate, according to OIE Stards, new procedures for diagnosis control of the designated pathogens or disees 6. Did your laboratory develop new diagnostic methods validated according to OIE Stards for the designated pathogen or disee? 4 OIE Reference Laboratory Reports Activities, 2016
5 7. Did your laboratory develop new vaccines according to OIE Stards for the designated pathogen or disee? ToR 4: To provide diagnostic testing facilities,, where appropriate, scientific technical advice on disee control meures to OIE Member Countries 8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? Name of OIE Member Country seeking sistance Date (month). samples received for provision of diagnostic support. samples received for provision of confirmatory diagnoses CHINA (PEOPLE'S REP. OF) February, April, June, August, September, vember, December FRANCE January 4 0 UNITED ARAB EMIRATES January Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? Name of the OIE Member Country receiving a technical consultancy Purpose How the advice w provided PAKISTAN Surveillance Remote - EGYPT Virus characterisation Remote - ToR 5: To carry out /or coordinate scientific technical studies in collaboration with other laboratories, centres or organisations 10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? OIE Reference Laboratory Reports Activities,
6 Title of the study Duration Purpose of the study Partners (Institutions) OIE Member Countries involved other than your country OIE Twinning Programme for equine influenza 3 years To improve surveillance & diagnosis of equine influenza in. The long term aim is to establish a new OIE reference laboratory for equine influenza in India National Research Centre on Equines, Hisar INDIA Surveillance for equine influenza ongoing To monitor horses for exposure to equine influenza University of Veterinary & Animal Sciences, Lahore PAKISTAN ToR 6: To collect, process, analyse, publish disseminate epizootiological data relevant to the designated pathogens or disees 11. Did your Laboratory collect epizootiological data relevant to international disee control? 12. Did your laboratory disseminate epizootiological data that had been processed analysed? 13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: 3 1. Alves Beuttemmüller E, Woodward A, Rh A, Dos Santos Ferraz LE, Fernes Alfieri A, Alfieri AA, Elton D. Characterisation of the epidemic strain of H3N8 equine influenza virus responsible for outbreaks in South a in Virol J Mar 19;13: Fougerolle S, Legr L, Garrett D, Bir I, Foursin M, D'Ablon X, Bayssat P, Newton RJ, Pronost S, Paillot R. Influential factors inducing suboptimal humoral response to vector-bed influenza immunisation in Thoroughbred foals. Vaccine Jul 19;34(33): Paillot R, Rh NL, Garrett D, Prowse-Davis L, Montesso F, Cullinane A, Lemaitre L, Thibault JC, Wittreck S, Dancer A. How to Meet the Lt OIE Expert Surveillance Panel Recommendations on Equine Influenza (EI) Vaccine Composition: A Review of the Process Required for the Recombinant Canarypox-Bed EI Vaccine. Pathogens v 25;5(4). b) International conferences: 0 c) National conferences: 0 6 OIE Reference Laboratory Reports Activities, 2016
7 d) Other: (Provide website address or link to appropriate information) 87 Rapid notification of outbreaks: 13 notifications including UK international outbreaks UK & international outbreaks reported on Equiflunet website: International Collating Centre disee reports: 9 interim reports issued for EIV occurrence, sent by made publically available on the AHT website. Routine summary reports were made available quarterly. Telltail text alerts to mobile phones 7 alerts for the UK 7 outbreaks reported on Worms & Germs map: 1 outbreak reported on Health Map: Increing awareness of equine influenza: in collaboration with Merial Animal Health part of flu awareness month a series of videos were made available on YouTube (April 2016) Webinar presentation: Infectious disee prevention control: effective strategies for equine premises, recorded in March 2016 presented to mark the launch of the EquiBioSafe App in summer Presented by Richard Newton. Participation in international training workshops: Oral presentations - a series of 6 talks w given on equine influenza, virus isolation, diagnostic says, ISO accreditation, surveillance vaccines, presented at the Surveillance Diagnostics for Equine Influenza Workshop at the National Research Centre on Equines, Hisar, India, February Presented by Debra Elton & Liz Medcalf. Oral presentation: Overview of surveillance horizon scanning for equine infectious disees, presented at the Bic Horse Husbry, Disee Surveillance Training Workshop, Hong Kong, vember Presented by Richard Newton. Oral presentation: Simple concepts for prevention control of equine infectious disees introduction to diagnostic testing, presented at the Bic Horse Husbry, Disee Surveillance Training Workshop, Hong Kong, vember Presented by Richard Newton. Oral presentation: Equine influenza: epidemiology, diagnosis, surveillance control, presented at the Bic Horse Husbry, Disee Surveillance Training Workshop, Hong Kong, vember Presented by Richard Newton. Sharing of isolate sequence data: via GISAID 24 HA 22 NA sequences made available. ToR 7: To provide scientific technical training for personnel from OIE Member Countries To recommend the prescribed alternative tests or vaccines OIE Stards 14. Did your laboratory provide scientific technical training to laboratory personnel from other OIE Member Countries? a) Technical visits: 0 b) Seminars: 6 c) Hs-on training courses: 1 d) Internships (>1 month): 0 OIE Reference Laboratory Reports Activities,
8 Type of technical training provided (a, b, c or d) Country of origin of the expert(s) provided with training. participants from the corresponding country b India, Afghanistan, Bangladesh, Bhutan, Sri Lanka, Nepal 15 c India, Afghanistan, Bangladesh, Bhutan, Sri Lanka, Nepal 15 ToR 8: To maintain a system of quality surance, biosafety biosecurity relevant for the pathogen the disee concerned 15. Does your laboratory have a Quality Management System certified according to an International Stard? Quality management system adopted ISO/IEC Certificate scan (PDF, JPG, PNG format) ISO Accreditation Certificate (4649) (2-18Sep12).pdf 16. Is your laboratory accredited by an international accreditation body? Test for which your laboratory is accredited qrt-pcr Accreditation body ILAC 17. Does your laboratory maintain a biorisk management system for the pathogen the disee concerned? (See Manual of Diagnostic Tests Vaccines for Terrestrial Animals, Chapter 1.1.4) ToR 9: To organise participate in scientific meetings on behalf of the OIE 18. Did your laboratory organise scientific meetings on behalf of the OIE? 19. Did your laboratory participate in scientific meetings on behalf of the OIE? 8 OIE Reference Laboratory Reports Activities, 2016
9 Title of event Date (mm/yy) Location Role (speaker, presenting poster, short communications) Title of the work presented Meeting of Expert Surveillance Paenl for equine influenza 02/16 Paris participant equine influenza in the UK, 2015 ToR 10: To establish maintain a network with other OIE Reference Laboratories designated for the same pathogen or disee organise regular inter-laboratory proficiency testing to ensure comparability of results 20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disee? 21. W your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disee by organising or participating in proficiency tests? Purpose of the proficiency tests: 1 Role of your Reference Laboratory (organiser/ participant). participants Participating OIE Ref. Labs/ organising OIE Ref. Lab. HI: maintenance of ISO accreditation for one participant, stardisation of say between reference laboratories Organiser 2 Irish Equine Centre/Animal Health Trust Diagnostic qrt-pcr say: maintenance of ISO accreditation, stardisation of say between reference laboratories Organiser 2 Irish Equine Centre/Animal Health Trust SRH:maintenance of ISO accreditation for one participant, stardisation of say between reference laboratories Organiser 2 Irish Equine Centre/Animal Health Trust 1 validation of a diagnostic protocol: specify the test; quality control of vaccines: specify the vaccine type, etc. 22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disee on scientific research projects for the diagnosis or control of the pathogen of interest? OIE Reference Laboratory Reports Activities,
10 Title of the project or contract Scope Name(s) of relevant OIE Reference Laboratories Sequence analysis & antigenic characterisation of EIV isolates Sequence analysis of virus isolates from the USA, antigenic characterisation by HI say for the purpose of monitoring antigenic drift Gluck Equine Research Center International Surveillance of equine influenza Review of surveillance data, recommendation of suitable vaccine strains Gluck Equine Research Centre, Irish Equine Centre, Free University of Berlin Validation of qpcr say to OIE stards First stage completed - testing nal swab samples against the gold stard ELISA say Irish Equine Centre ToR 11: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens disees to ensure equivalence of results 23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disee? te: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: see point 1.3 ToR 12: To place expert consultants at the disposal of the OIE 24. Did your laboratory place expert consultants at the disposal of the OIE? 25. Additional comments regarding your report: 10 OIE Reference Laboratory Reports Activities, 2016
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