OIE Reference Laboratory Reports Activities
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1 OIE Reference Laboratory Reports Activities Activities in 2016 This report has been submitted : :06:20 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Bluetongue Address of laboratory: Ash Road, Pirbright Woking, Surrey, GU24 0NF UNITED KINGDOM Tel.: Fax: address: peter.mertens@pirbright.ac.uk Website: Name (including Title) of Head of Laboratory (Responsible Official): Dr Bryan Charleston The Pirbright Institute Name (including Title and Position) of OIE Reference Expert: Peter Mertens Which of the following defines your laboratory? Check all that apply: Research OIE Reference Laboratory Reports Activities,
2 ToR 1: To use, promote and disseminate diagnostic methods validated according to OIE Standards 1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as disease diagnosis, screening of animals for export, surveillance, etc.? (t for quality control, proficiency testing or staff training) Diagnostic Test Indicated in OIE Manual (/) Total number of test performed last year Indirect diagnostic tests Nationally Internationally ELISA yes Direct diagnostic tests Nationally Internationally real time RT-PCR yes VI yes 0 10 ToR 2: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease. 2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE? 3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents to OIE Member Countries? 2 OIE Reference Laboratory Reports Activities, 2016
3 Type of reagent available Related diagnostic test Produced/ provide Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg). of recipient OIE Member Countries Region of recipients 21 various strains of BTV-8 various provided 21 x 1ml 0 1 Asia and Pacific Middle East BTV-16 strain RSArrrr/16, BTV-21 strain RSArrrr/21 various provided 0 2 x 2ml 1 Asia and Pacific Middle East BTV-4 strain HUN2014/01, BTV-8 strain FRA2015/01, BTV-26 strain KUW2010/02 various provided 0 3 x 2ml 1 Asia and Pacific Middle East BTV antisera for serotypes 1-24 and 26 SNT provided 0 25 x 10ml 1 Asia and Pacific Middle East RNA extracted from BTV serotypes 1-24 and 26 PCR provided 0 25 x 200ul 1 Asia and Pacific Middle East 4. Did your laboratory produce vaccines? 5. Did your laboratory supply vaccines to OIE Member Countries? OIE Reference Laboratory Reports Activities,
4 ToR 3: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases 6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease? 7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? ToR 4: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries 8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? Name of OIE Member Country seeking assistance Date (month). samples received for provision of diagnostic support. samples received for provision of confirmatory diagnoses GEORGIA January 0 8 MONTENEGRO August 5 0 CYPRUS September 0 16 CYPRUS vember Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? 4 OIE Reference Laboratory Reports Activities, 2016
5 Name of the OIE Member Country receiving a technical consultancy Purpose How the advice was provided GEORGIA troubleshooting false positive test results in Georgia and telephone ROMANIA Pooling of samples SLOVENIA which assay to use as front line test, pooling of samples, internal controls TANZANIA troubleshooting and interpretation of PCR assays telephone and face to face BRAZIL transfer of diagnostic technologies for detection and serotyping of BTV by correspondence and during scientific visits to Minas Gerais Brazil ToR 5: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations 10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? OIE Reference Laboratory Reports Activities,
6 Title of the study Duration Purpose of the study Partners (Institutions) OIE Member Countries involved other than your country characterization of BTV-8 3 months to analyse the sequences of BTV-8 from France ANSES / INRA Maissons Alfort, Paris FRANCE Understanding pathogen, livestock, environment interactions involving bluetongue virus (PALE- Blu) 36 months advance knowledge to combat BTV spread in and surrounding countries ANSES / INRA, CIRAD, IZSAM, ERGO, FLI, ULB, INIA, CVI, UGLA- CVR, Kimron, UCM, SVA, KAU, IAV, ILRI, ISRA, BELGIUM FRANCE GERMANY ISRAEL ITALY KENYA MOROCCO SENEGAL SPAIN SWEDEN THE NETHERLANDS TURKEY Improvement of diagnostic system for Bluetongue and characterization of Korean isolates collaboration with THE ANIMAL AND PLANT QUARANTINE AGENCY ( QIA ) 3 years Improvement of diagnostic system for Bluetongue and characterization of Korean BTV isolates THE ANIMAL AND PLANT QUARANTINE AGENCY ( QIA ) 1 KOREA (REP. OF). reference collections and molecular epidemiology studies for important arboviral pathogens of livestock in India 4 years Generate reference collections and molecular epidemiology studies for important arboviral pathogens of livestock in India JIC (UK), UGLA-CVR (UK), LUVA (Hisar), IVRI (Bareilly), Ella Foundation Hyderabad, CVS Hyderabad, CBRI, Andhra Pradesh, INDIA BRAZILIAN INTERNATIONAL NETWORK FOR DETECTION OF ORBIVIRUSES AND CULICOIDES (BRAIN- DOC) 3 years to transfer diagnostic technology for BTV to UFMG Belo Horizonte Minas Gerias, Brazil UFMG Belo Horizonte BRAZIL ToR 6: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases 11. Did your Laboratory collect epizootiological data relevant to international disease control? 12. Did your laboratory disseminate epizootiological data that had been processed and analysed? 6 OIE Reference Laboratory Reports Activities, 2016
7 13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: 9 Dual Infection with Bluetongue Virus Serotypes and First-Time Isolation of Serotype 5 in India. Hemadri D, Maan S, Chanda MM, Rao PP, Putty K, Krishnajyothi Y, Reddy GH, Kumar V, Batra K, Reddy YV, Maan NS, Reddy YN, Singh KP, Shivachandra SB, Hegde NR, Rahman H, Mertens PP. Transbound Emerg Dis Dec 21. doi: /tbed [Epub ahead of print]pmid: Genome Sequence of Bluetongue virus Serotype 17 Isolated in Brazil in Matos AC, Rosa JC, mikou K, Guimarães LL, Costa ÉA, Guedes MI, Driemeier D, Lobato ZI, Mertens PP. Genome Announc Oct 27;4(5). pii: e doi: /genomeA PMID: OIEBTLABNET: the web-based network of the OIE Bluetongue Reference Laboratories. Conte A, Daniels P, Goffredo M, Ostlund EN, Di Lorenzo A, Savini L, Tora S, Lubisi BA, Calistri P, Bruno R, Di Sabatino D, Ippoliti C, Monaco F, Mertens PP, Lorusso A, Capobianco A, Ripani A, MacLachlan NJ, Savini G. Vet Ital Sep 30;52(3-4): doi: /VetIt PMID: Development and Evaluation of Real Time RT-PCR Assays for Detection and Typing of Bluetongue Virus. Maan S, Maan NS, Belaganahalli MN, Potgieter AC, Kumar V, Batra K, Wright IM, Kirkland PD, Mertens PP. PLoS One Sep 23;11(9):e doi: /journal.pone PMID: Isolation of Bluetongue Virus 24 from India - An Exotic Serotype to Australasia. Krishnajyothi Y, Maan S, Kandimalla K, Maan NS, Tutika RB, Reddy YV, Kumar A, Mrunalini N, Reddy GH, Putty K, Ahmed SM, Reddy YN, Hemadri D, Singh KP, Mertens PP, Hegde NR, Rao PP. Transbound Emerg Dis Aug;63(4): doi: /tbed PMID: Reverse transcription loop-mediated isothermal amplification assays for rapid identification of eastern and western strains of bluetongue virus in India. Maan S, Maan NS, Batra K, Kumar A, Gupta A, Rao PP, Hemadri D, Reddy YN, Guimera M, Belaganahalli MN, Mertens PP. J Virol Methods Aug;234: doi: /j.jviromet PMID: Identification of the Genome Segments of Bluetongue Virus Serotype 26 (Isolate KUW2010/02) that Restrict Replication in a Culicoides sonorensis Cell Line (KC Cells). Pullinger GD, Guimerà Busquets M, mikou K, Boyce M, Attoui H, Mertens PP. PLoS One Feb 18;11(2):e doi: /journal.pone PMID: Development of Real-Time RT-PCR Assays for Detection and Typing of Epizootic Haemorrhagic Disease Virus. Maan NS, Maan S, Potgieter AC, Wright IM, Belaganahalli M, Mertens PP. Transbound Emerg Dis Feb 17. doi: /tbed [Epub ahead of print] PMID: Using shared needles for subcutaneous inoculation can transmit bluetongue virus mechanically between ruminant hosts. Darpel KE, Barber J, Hope A, Wilson AJ, Gubbins S, Henstock M, Frost L, Batten C, Veronesi E, Moffat K, Carpenter S, Oura C, Mellor PS, Mertens PP. Sci Rep Feb 8;6: doi: /srep PMID: b) International conferences: 2 EVAG meeting ILRI 29th June 2016, Virology meeting - 17th February 2016 Bareilly India c) National conferences: 2 SGM May 2016 Emerging Viral Diseases ttingham d) Other: (Provide website address or link to appropriate information) 1 molecular epidemiology report for samples submitted in 2016 from Cyprus OIE Reference Laboratory Reports Activities,
8 ToR 7: To provide scientific and technical training for personnel from OIE Member Countries To recommend the prescribed and alternative tests or vaccines as OIE Standards 14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries? a) Technical visits: 10 b) Seminars: 0 c) Hands-on training courses: 8 d) Internships (>1 month): 0 Type of technical training provided (a, b, c or d) Country of origin of the expert(s) provided with training. participants from the corresponding country a Tanzania 8 c Tanzania 8 a India 1 a Republic of Korea 1 ToR 8: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned 15. Does your laboratory have a Quality Management System certified according to an International Standard? Quality management system adopted ISO/IEC17025 Certificate scan (PDF, JPG, PNG format) scope of accreditation UKAS 2016.pdf 16. Is your laboratory accredited by an international accreditation body? Test for which your laboratory is accredited RT-PCR ELISA virus isolation Accreditation body United Kingdom accrediation service (UKAS) UKAS UKAS 8 OIE Reference Laboratory Reports Activities, 2016
9 17. Does your laboratory maintain a biorisk management system for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Chapter 1.1.4) ToR 9: To organise and participate in scientific meetings on behalf of the OIE 18. Did your laboratory organise scientific meetings on behalf of the OIE? 19. Did your laboratory participate in scientific meetings on behalf of the OIE? ToR 10: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results 20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease? 21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests? Purpose of the proficiency tests: 1 Role of your Reference Laboratory (organiser/ participant). participants Participating OIE Ref. Labs/ organising OIE Ref. Lab. Serological and molecular detection methods for BTV organiser 44 IZS, Italy, South 1 validation of a diagnostic protocol: specify the test; quality control of vaccines: specify the vaccine type, etc. 22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest? OIE Reference Laboratory Reports Activities,
10 ToR 11: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results 23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease? te: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: see point 1.3 Purpose for inter-laboratory test comparisons 1. participating laboratories Region(s) of participating OIE Member Countries Serological and molecular detection methods for BTV 44 Asia and Pacific Middle East ToR 12: To place expert consultants at the disposal of the OIE 24. Did your laboratory place expert consultants at the disposal of the OIE? Kind of consultancy Location Subject (facultative) Reviewing documents TPI-UK OIE Guidelines for BTV diagnosis 25. Additional comments regarding your report: 10 OIE Reference Laboratory Reports Activities, 2016
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