OIE Reference Laboratory Reports Activities
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1 OIE Reference Laboratory Reports Activities Activities in 2013 This report has been submitted : :17:32 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Crimean-Congo haemorrhagic fever Address of laboratory: Dr. Michèle BOULOY OIE Reference Laboratory for Crimean Congo Haemorrhagic Fever Virus (CCHFV) & Rift Valley Fever Virus (RVFV) Institut Pasteur 25 rue du Dr Roux Paris cedex 15 FRANCE Tel.: Fax: address: mbouloy@pasteur.fr website: Name (including Title) of Head of Laboratory (Responsible Official): Head CCHFV Laboratory Dr. Michèle Bouloy, Institut Pasteur, Paris, France Two Partners : - Dr. ël Tordo, Antiviral Strategies Unit, WHOCC- VHF, Institut Pasteur, Paris - Dr. Philippe Marianneau, Virology Unit, NRL- RVFV, ANSES-Laboratory, Lyon Name (including Title and Position) of OIE Reference Expert: Dr. Michèle Bouloy Department of Virology, Institut Pasteur, Paris, France Which of the following defines your laboratory? Check all that apply: Academic OIE Reference Laboratory Reports Activities,
2 ToR: To use, promote and disseminate diagnostic methods validated according to OIE Standards 1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as disease diagnosis, screening of animals for export, surveillance, etc.? (t for quality control, proficiency testing or staff training) Diagnostic Test Indicated in OIE Manual (/) Total number of test performed last year Indirect diagnostic tests Nationally Internationally Competition ELISA 0 under development IgG ELISA 0 about 150 (test validation) Direct diagnostic tests Nationally Internationally RT-PCR (nested) 0 0 ToR: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease. 2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE? 3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents to OIE Member Countries? 2 OIE Reference Laboratory Reports Activities, 2013
3 Type of reagent available Related diagnostic test Produced/ provide Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg). of recipient OIE Member Countries Region of recipients (+) and (-) sera for CCHFV from bovine, ovine, camelidae Serology by ELISA provide 0.5 ml 0.5 ml 2 Africa Americas Asia and Pacific Europe Middle East Immune ascite of mice Serology by ELISA produced 0.1 ml 0.1 ml 1 Africa Americas Asia and Pacific Europe Middle East (+) and (-) sera for CCHFV from immunized monkey Serology by ELISA produced 0.5 ml 0.5 ml 2 Africa Americas Asia and Pacific Europe Middle East 4. Did your laboratory produce vaccines? 5. Did your laboratory supply vaccines to OIE Member Countries? ToR: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases 6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease? 7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? Name of the new test or diagnostic method or vaccine developed Description and References (Publication, website, etc.) Development of serological tests with recombinant CCHFV N proteins (from Greece, China, Africa) produced in S2 cells (Drosophila). Application for both ELISA and Luminex (Muliplex serology). A KIT is in development (EU program EDENEXT) in collaboration with the Friedrich Loeffler Institute (Germany) and IdVet (Company for Veterinary diagnosis in Montpellier) OIE Reference Laboratory Reports Activities,
4 ToR: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries 8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? 9. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? Name of the OIE Member Country receiving a technical consultancy Purpose How the advice was provided MOROCCO Evaluation of Camel sera through collaborative work ToR: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations 10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? Title of the study Duration Purpose of the study Partners (Institutions) EU-FP7 program EDENEXT Tick-Borne Diseases up to end 2014 Developement of an ELISA kit for survey of CCHFV serology in cattle FLI, Germany (leader) Inst. Pasteur, France IDVet, Montpellier ToR: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases 11. Did your Laboratory collect epizootiological data relevant to international disease control? 12. Did your laboratory disseminate epizootiological data that had been processed and analysed? 13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) 4 OIE Reference Laboratory Reports Activities, 2013
5 a) Articles published in peer-reviewed journals: 2 - Filippone C, Marianneau P, Murri S, Mollard N, Avsic-Zupanc T, Chinikar S, Desprès P, Caro V, Gessain A, Berthet N, Tordo N. Molecular diagnostic and genetic characterization of highly pathogenic viruses: application during Crimean-Congo haemorrhagic fever virus outbreaks in Eastern Europe and the Middle-East Clinical Microbiology and Infection. 19(2):E Tordo N, Marianneau P. La fièvre de Crimée Congo Bulletin des GTV (n spécial) b) International conferences: 5 - Tordo N, uveaux outils de diagnostic génétiques et sérologiques. Infections émergentes et ré-émergentes: apport des Biotechnologies. Rabat, Morrocco, 30 v. - 1 Dec Tordo N, Fièvres Hémorragiques virales. Infections émergentes et ré-émergentes: apport des Biotechnologies. Rabat, Morrocco, 30 v. - 1 Dec Filippone C., Tordo N. Diagnostic of Emerging Viruses. Enfermedades emergentes, el papel zoonótico de pequeños mamíferos y ectoparásitos. Mérida, Yucatán, Mexico, 29 v Participation to the Annual Meeting "Edenext"-2013, Barcelona, March, Organization of the "European Virolgy Congress", Sept 2013, Lyon (1200 participants, Session dedicated to emerging and arboviruses including CCHFV). c) National conferences: 2 - Ben-Mechlia M, Filippone1 C, Marianneau M, Murri S, Ermonval M, Jallet C,Desprès P, Gessain A, Tordo N. Large screening serological and molecular methods for studying the diversity of Bunyaviruses in their reservoir hosts and in Humans. Journées du Département de Virologie de l'institut Pasteur, Ilse-sur Sorgues, France octobre Organization of the "Journées Francophones de Virologie", April 2013, Insitut Pasteur Paris (400 participants, Session dedicated to emerging viruses. d) Other: (Provide website address or link to appropriate information) 3 Courses: - Course of Systematic Virology, Institut Pasteur : Emergence et diagnistic des fièvres hémorrhagiques virales (3h) - Master of Virology, University Paris 11 : Les virus émergents (3h) - Master of Virology, University Paris 6 : Mecanismes de l'émergence virale (3h) ToR: To provide scientific and technical training for personnel from OIE Member Countries To recommend the prescribed and alternative tests or vaccines as OIE Standards 14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries? ToR: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned 15. Does your laboratory have a Quality Management System certified according to an International Standard? OIE Reference Laboratory Reports Activities,
6 Explain Quality Management System in adoption process or currently in place CCHFV is considered as possibly subject of "dual use". For that reason, the virus itself but also antigenic or genetic subproducts are considered in the "MOT"'s group (MicroOganisms & Toxins) and therefore submitted to a constant surveillance (reports, autorizations for Detention, Experiments, Import, Export..) by the French control Agency (ANSM). Laboratories at the required Biosafety level (BSL2/3) are used for virus/subproduct handling. 16. Is your laboratory accredited by an international accreditation body? 17. Does your laboratory maintain a biorisk management system for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2012, Chapter or Manual of Diagnostic Tests for Aquatic Animals 2012, Chapter 1.1.1) ToR: To organise and participate in scientific meetings on behalf of the OIE 18. Did your laboratory organise scientific meetings on behalf of the OIE? 19. Did your laboratory participate in scientific meetings on behalf of the OIE? ToR: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results 20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease? 21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests? 22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific 6 OIE Reference Laboratory Reports Activities, 2013
7 research projects for the diagnosis or control of the pathogen of interest? Title of the project or contract Scope Name(s) of relevant OIE Reference Laboratories EU-FP7 program EDENEXT Tick- Borne Diseases EU-FP7 program EDENEXT Tick-Borne Diseases Developement of an ELISA kit for survey of CCHFV serology in cattle Friedrich Loeffler Institute, Germany (Martin Groschup) ToR: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results 23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease? te: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: see point 1.3 ToR: To place expert consultants at the disposal of the OIE 24. Did your laboratory place expert consultants at the disposal of the OIE? 25. Additional comments regarding your report: OIE Reference Laboratory Reports Activities,
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