OIE Reference Laboratory Reports Activities
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1 OIE Reference Laboratory Reports Activities Activities in 206 This report h been submitted : :27:9 Name of disee (or topic) for which you are a designated OIE Reference Laboratory: Swine influenza Address of laboratory: Via Bianchi Brescia ITALY Tel.: +39 (0) Fax: +39 (0) address: emanuela.foni@izsler.it Website: Name (including Title) of Head of Laboratory (Responsible Official): Prof. Stefano Cinotti Name (including Title Position) of OIE Reference Expert: Emanuela Foni, DVM, Veterinary Manager Which of the following defines your laboratory? Check all that apply: Governmental OIE Reference Laboratory Reports Activities, 206
2 ToR : To use, promote disseminate diagnostic methods validated according to OIE Stards. Did your laboratory perform diagnostic tests for the specified disee/topic for purposes such disee diagnosis, screening of animals for export, surveillance, etc.? (t for quality control, proficiency testing or staff training) Diagnostic Test Indicated in OIE Manual (/) Total number of test performed lt year Indirect diagnostic tests Nationally Internationally HI test Direct diagnostic tests Nationally Internationally PCR test 397 Viral Isolation on Eggs 78 0 Viral Isolation on Cells 27 0 ToR 2: To develop reference material in accordance with OIE requirements, implement promote the application of OIE Stards. To store distribute to national laboratories biological reference products any other reagents used in the diagnosis control of the designated pathogens or disee. 2. Did your laboratory produce or supply imported stard reference reagents officially recognised by the OIE? 3. Did your laboratory supply stard reference reagents (non OIE-approved) /or other diagnostic reagents to OIE Member Countries? 2 OIE Reference Laboratory Reports Activities, 206
3 Type of reagent available Related diagnostic test Produced/ provide Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg). of recipient OIE Member Countries Region of recipients Virus:HNpdm09 A/sw/It/282866/203 test produced 60ml (ready to use 2ml( ) 2 H3N2: A/sw/It/3349/203 produced 40ml(ready to use) 2ml 2 HN2: A/sw/It/284922/2009 produced 30 ml(ready to use) 2ml 2 Virus:HN A/sw/It/3368/203 produced 2ml Hyperimmune serum:hn A/sw/It/3368/203 produced 6ml Hyperimmune serum H3N2: A/sw/It/3349/203 6ml OIE Reference Laboratory Reports Activities, 206 3
4 Hyperimmune serum HN2 A/sw/It/284922/2009 6ml Hyperimmune serum HN pdm09 A/sw/it/ /203 6ml Virus HN A/sw/It/7704/200 experimental infection,5 ml ( Virus D/sw/It/ /205 HI Virus isolation method development 2 ml () Virus HN A/swine/Italy/233405/202 to BIOBANKING Veterinary Resources OIE Collaborative Centre 0 ml () The reagents Virus HN A/swine /Italy/37775/200 to BIOBANKING Veterinary Resources OIE Collaborative Centre 0 ml () The reagents 4 OIE Reference Laboratory Reports Activities, 206
5 Virus HN Virus HN2 A/swine/Italy/275736/200 to BIOBANKING Veterinary Resources OIE Collaborative Centre 0 ml () The reagents Virus N3N2 A/swine/Italy/302749/200 to BIOBANKING Veterinary Resources OIE Collaborative Centre 0 ml () The reagents Virus H3N2 A/swine/Italy/55925/20 to BIOBANKING Veterinary Resources OIE Collaborative Centre 0 ml () The reagents 4. Did your laboratory produce vaccines? 5. Did your laboratory supply vaccines to OIE Member Countries? ToR 3: To develop, stardise validate, according to OIE Stards, new procedures for diagnosis control of the designated pathogens or disees 6. Did your laboratory develop new diagnostic methods validated according to OIE Stards for the designated pathogen or disee? 7. Did your laboratory develop new vaccines according to OIE Stards for the designated pathogen or disee? OIE Reference Laboratory Reports Activities, 206 5
6 ToR 4: To provide diagnostic testing facilities,, where appropriate, scientific technical advice on disee control meures to OIE Member Countries 8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? Name of OIE Member Country seeking sistance Date (month). samples received for provision of diagnostic support. samples received for provision of confirmatory diagnoses HUNGARY March 20 0 ROMANIA vember 20 0 ITALY March 5 HUNGARY April 75 HUNGARY May 70 HUNGARY July 30 HUNGARY August 30 HUNGARY September 30 HUNGARY vember Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? Name of the OIE Member Country receiving a technical consultancy Purpose How the advice w provided SERBIA sharing information on reagents primer to be used for confirmatory subtyping test fo swine influenza virus serological investigation ROMANIA sharing information on reagents primer to be used for confirmatory subtyping test fo swine influenza virus serologucal investigation ITALY Genetic characterization of HN swine influenza virus isolated from human Local meeting 6 OIE Reference Laboratory Reports Activities, 206
7 ToR 5: To carry out /or coordinate scientific technical studies in collaboration with other laboratories, centres or organisations 0. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? Title of the study Duration Purpose of the study Partners (Institutions) OIE Member Countries involved other than your country CoVetLab Sept 206- March 207 Next Generation Sequencing for genetic characterisation of swine influenza viruses in APHA,DTU,ANSES, CVI- Lelystad, SVA, FLI DENMARK FRANCE GERMANY SWEDEN THE NETHERLANDS UNITED KINGDOM ToR 6: To collect, process, analyse, publish disseminate epizootiological data relevant to the designated pathogens or disees. Did your Laboratory collect epizootiological data relevant to international disee control? 2. Did your laboratory disseminate epizootiological data that had been processed analysed? 3. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: 3 -Chiapponi C, Faccini S, de Mattia A, Baioni L, Barbieri I, Rosignoli C, Nigrelli A. Foni E. Detection of influenza D virus among swine cattle, Italy. Emerg Infect Dis. 206 Feb 22(2): Baumann J, Kousi NM, Foni E, Klenk H-D, Matrosovich M HN swine influenza viruses differ from avian precursors by a higher ph optimum of membrane fusion. J Virol 90: doi:0.28/jvi Henritzi D., Zhao N., Starick E, Simon G., Krog J.S., Larsen L.E., Reid S.M., Brown I.H., Chiapponi C., Foni E., Wacheck S., Schmid P., Beer M., Hoffmann B., Harder T.C. (206). Rapid detection subtyping of an swine influenza viruses in porcine clinical samples by hemagglutinin- neuraminide-specific tetra- triplex real-time RT-PCRs. Influenza Other Respir Viruses. 206 Jul. doi: 0./irv [Epub ahead of print b) International conferences: 3 - Foni E., Chiapponi C., Faccini S., Baioni L., De Mattia A., Barbieri I., Rosignoli C., Merenda M., Nigrelli A. (206) Circulation of the novel influenza virus, proposed influenza D virus, in Italian pig farms. Proceedings of 24th International Pig Veterinary Society Congress. Dublin, June 7th-0th 206, Reid S.M., Russell C., Williamson S., Simon G., Loeffen W, Larsen S., Zohari S., Chiapponi C., Harder T., Gorin S., OIE Reference Laboratory Reports Activities, 206 7
8 Krog J.S., Foni E., Brookes S. M., Brown I. H. (206) Development validation of real-time RT-PCR protocols for sub-typing swine influenza viruses. Proceedings of 24th International Pig Veterinary Society Congress. Dublin, June 7th-0th 206, Chiapponi C., Faccini S., Bolzoni L., Merenda M., Baioni L., Manfredi R., Zanni I., Rosignoli C., De Mattia A., Nigrelli A., Foni E. (206) Circulation of influenza D virus in cattle in Italy.Proccedings of 6th an Congress of Virology, October 9-22, 206, Hamburg, Germany. P.26-3 c) National conferences: 2 -Foni E., Chiapponi C., Faccini S., Baioni L., Barbieri I., Rosignoli C., Merenda M., Zanni I., Manfredi R., Sri G., Nigrelli A. Sulla circolazione di virus influenza D in suini di allevamenti del rd Italia. Atti del XLII meeting Annuale della Società Italiana di Patologia ed Allevamento dei Suini. Montichiari (BS)0- Marzo 206, Rosignoli C, Merenda M., Faccini S, Chiapponi C, De_Mattia A, Bufalo G, Garbarino C, Baioni L, Nigrelli AD, Foni E (206) Virus influenza D e malattia respiratoria del bovino : indagine in allevamenti italiani. Buiatria (206). - 6 p. - 6 bib ref d) Other: (Provide website address or link to appropriate information) 0 ToR 7: To provide scientific technical training for personnel from OIE Member Countries To recommend the prescribed alternative tests or vaccines OIE Stards 4. Did your laboratory provide scientific technical training to laboratory personnel from other OIE Member Countries? a) Technical visits: 0 b) Seminars: 0 c) Hs-on training courses: d) Internships (> month): 0 Type of technical training provided (a, b, c or d) Country of origin of the expert(s) provided with training. participants from the corresponding country c Romania 2 ToR 8: To maintain a system of quality surance, biosafety biosecurity relevant for the pathogen the disee concerned 5. Does your laboratory have a Quality Management System certified according to an International Stard? Quality management system adopted EN ISO/IEC 7025:2005 Certificate scan (PDF, JPG, PNG format) Accreditation Certificate.pdf 8 OIE Reference Laboratory Reports Activities, 206
9 6. Is your laboratory accredited by an international accreditation body? 7. Does your laboratory maintain a biorisk management system for the pathogen the disee concerned? (See Manual of Diagnostic Tests Vaccines for Terrestrial Animals, Chapter..4) ToR 9: To organise participate in scientific meetings on behalf of the OIE 8. Did your laboratory organise scientific meetings on behalf of the OIE? 9. Did your laboratory participate in scientific meetings on behalf of the OIE? ToR 0: To establish maintain a network with other OIE Reference Laboratories designated for the same pathogen or disee organise regular inter-laboratory proficiency testing to ensure comparability of results 20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disee? 2. W your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disee by organising or participating in proficiency tests? Purpose of the proficiency tests: Role of your Reference Laboratory (organiser/ participant). participants Participating OIE Ref. Labs/ organising OIE Ref. Lab. Proficency test Real time PCR for M gene of Influenza virus partecipant 3 OIE Reference Laboratory for Avian Influenza IzsVe Padua validation of a diagnostic protocol: specify the test; quality control of vaccines: specify the vaccine type, etc. 22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disee on scientific research projects for the diagnosis or control of the pathogen of interest? OIE Reference Laboratory Reports Activities, 206 9
10 Title of the project or contract Scope Name(s) of relevant OIE Reference Laboratories CoVetLab Next Generation Sequencing for genetic characterisation of swine influenza viruses in APHA (UK) ToR : To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens disees to ensure equivalence of results 23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disee? te: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: see point.3 ToR 2: To place expert consultants at the disposal of the OIE 24. Did your laboratory place expert consultants at the disposal of the OIE? 25. Additional comments regarding your report: 0 OIE Reference Laboratory Reports Activities, 206
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