AMDCC Neuropathy Core March Eva L. Feldman, M.D., Ph.D Department of Neurology JDRF Center University of Michigan

Transcription

1 AMDCC Neuropathy Core March 6 Eva L. Feldman, M.D., Ph.D Department of Neurology JDRF Center University of Michigan

2 AMDCC Definition of Neuropathy Evidence of clinical loss of sensory function Tail flick and hind paw withdrawal latencies (TF and HP) Electrophysiological evidence of nerve impairment Sensory and motor distal latencies and nerve conduction velocity (NCV) Anatomical evidence of nerve fiber loss Intraepidermal fiber density (IEFD

3 AMDCC Core Protocol Tail Blood Catheter Insertion Perfusion Tail Flick Hind Paw Urine Sample Organ Retrieval Eyes Anesthesia Cystometrogram Bladder Nerve Conductions Euthanasia Kidney Foot pads

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8 Intraepidermal Nerve Fiber Density 8

9 Sensory Tests C7BLKS db/db Mice TF * HP ± %GHb GHb *. db+ dbdb. db+ dbdb. db+ dbdb. TF. HP. GHb %GHb db+ dbdb. db+ dbdb. db+ dbdb * P <., ± P <. 9

10 Nerve Conduction Velocity C7BLKS db/db Mice TSNCV TDML SMNCV * milli 7 6 * 6 ± db+ dbdb db+ dbdb db+ dbdb TSNCV TDML SMNCV 7 6 milli 6 db+ dbdb db+ dbdb db+ dbdb * P <., ± P <.

11 IENF Density C7BLKS db/db Mice γ γ P <.

12 Neuropathy Advanced Phenotyping: TUNEL and Nitrotyrosine IHC γ γ P <.

13 Animal Models: Ongoing Phenotyping* Tail Flick Hind Paw every 8- weeks NCV Measured or 6 and/or weeks post-diabetes IEFD Measured at tissue harvest Biochemistry Measured at tissue harvest Advanced Neuropathy Phenotyping C7BLKS db/db X X X X X C7BLKS db/db ± resveratrol GCLC +/- db/db high fat diet GLUT tg db/db High fat diet Nestin Cre// SOD loxp/loxp X X NA X NA X Ongoing Ongoing Ongoing NA X X Ongoing Ongoing Ongoing X X X X Ongoing Akita STZ X X Ongoing Ongoing Ongoing Pdx +/+ and +/- NA X X X X RAGE Tg STZ Ongoing Ongoing Ongoing Ongoing Ongoing DbA STZ ± rosiglitazone Ongoing (only wk) Ongoing (only wk) X = completed NA = not applicable * Have phenotyped over mice since Ongoing Ongoing Ongoing

14 Sensory Tests GCLC +/- db/db High Fat Mice TF HP wt db+ wt dbdb gclc db+ gclc dbdb TF wt db+ wt dbdb gclc db+ gclc dbdb HP wt db+ wt dbdb gclc db+ gclc dbdb wt db+ wt dbdb gclc db+ gclc dbdb No Significant Differences

15 Nerve Conduction Velocity GCLC +/- db/db High Fat Mice TSNCV TDML SMNCV 6 milli wt db+ wt dbdb gclc db+ gclc dbdb wt db+ wt dbdb gclc db+ gclc dbdb wt db+ wt dbdb gclc db+ gclc dbdb TSNCV milli TDML 6 SMNCV wt db+ wt dbdb gclc db+ gclc dbdb wt db+ wt dbdb gclc db+ gclc dbdb wt db+ wt dbdb gclc db+ gclc dbdb No Significant Differences

16 Sensory Tests GLUT tg db/db High Fat Mice TF HP wt db+ wt dbdb tg db+ tg dbdb. wt db+ wt dbdb tg db+ tg dbdb TF HP 7... wt db+ wt dbdb tg db+ tg dbdb. wt db+ wt dbdb tg db+ tg dbdb No Significant Differences 6

17 Nerve Conduction Velocity GLUT tg db/db High Fat Mice TSNCV TDML SMNCV 7 milli 6 wt db+ wt dbdb tg db+ tg dbdb wt db+ wt dbdb tg db+ tg dbdb wt db+ wt dbdb tg db+ tg dbdb TSNCV TDML SMNCV 7. milli.. wt db+ wt dbdb tg db+ tg dbdb. wt db+ wt dbdb tg db+ tg dbdb wt db+ wt dbdb tg db+ tg dbdb No Significant Differences 7

18 Sensory Tests Akita Mice TF HP γ wt C akita D wt C akita D TF HP γ P <. wt C akita D wt C akita D 8

19 Nerve Conduction Velocity Akita Mice TSNCV TDML SMNCV 6 wt C akita D milli wt C akita D wt C akita D TSNCV TDML SMNCV 6 wt C akita D milli wt C akita D wt C akita D No Significant Differences 9

20 Sensory Tests Akita Mice + rosiglitazone TF HP wt C akita D wt C akita D TF HP wt C akita D wt C akita D No Significant Differences

21 Nerve Conduction Velocity Pdx Mice TSNCV TDML SMNCV pdx +/+ pdx +/- milli pdx +/+ pdx +/- 6 γ pdx +/+ pdx +/- TSNCV pdx +/+ pdx +/- milli TDML pdx +/+ pdx +/- 6 SMNCV pdx +/+ pdx +/- γ P <.

22 Vanderbilt ENU/Akita Mice IENF Density IENF IENF fibers/mm ENU Akita fibers/mm ENU Akita

23 Nestin Cre// SOD loxp/loxp IENF Density A B d e d e C IENF IENF D IENF IENF fibers/mm fibers/mm No Significant Differences cre

24 Nestin Cre// SOD loxp/loxp TUNEL and Nitrotyrosine IHC A B TUNEL TUNEL TUNEL C TUNEL % Tunel Positive D Number of Pixels % Tunel Positive Anti-NT C7Bl6J α-nt Pixel Intensity.... No Significant Differences

25 AMDCC Neuropathy Core Eva L. Feldman, M.D., Ph.D JDRF Center University of Michigan Thanks to NIH/JDRF, EAC and AMDCC Investigators

26 C7BLKS db/db mice + resveratrol Strain Group Body Weight Plasma glucose Glycated hemoglobin (g) (mg/dl) (%) db/+ Untreated 6. ± ±.8. ±. mg/kg resveratrol 6. ± ±.6.7 ±. mg/kg resveratrol 7. ±. 8. ±..9 ±. db/db Untreated.7 ±.9*.8 ±.8*. ±.* mg/kg resveratrol. ±.*.7 ±.*. ±.* mg/kg resveratrol 6.6 ±.* 7. ± 9.6* 9. ±.* Values are ± SEM, *p <. versus untreated db+ mice; p <. versus untreated db/db mice; p <. versus mg/kg RES treated db/db mice. 6

27 C7BLKS db/db mice + resveratrol Strain Group Body Weight Glycated Hb Triglyceride SMNCV TSNCV (g) (%) (mg/dl) (m/sec) (m/sec) db/+ Untreated 6. ±.. ±.. ±.. ±. 8. ±. mg/kg res 7. ±..9 ±..6 ± ±. 7. ±. db/db Untreated.7 ±.9*. ±.* 69.9 ±.* 6.9 ±.9* 6. ±.7* mg/kg res 6.6 ±.* * 6.9 ± 8.*. ±.*.8 ±.* Evaluation of peripheral nerve function in db/+ and db/db mice treated with/without RES Values are ± SEM, *p <. versus untreated db+ mice. 7

28 Nerve Conduction Velocity Nestin Cre// SOD loxp/loxp Mice TSNCV TDML SMNCV # milli TSNCV milli TDML SMNCV # P <. 8

29 Sensory Tests Nestin Cre// SOD loxp/loxp Mice TF # HP % Total GHb GHb #.. TF HP GHb 7... % Total GHb. # P <. 9