ginseng & Bioconversion
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1 ginseng & Bioconversion Bio 1
2 Bio INCI Name : LACTOBACILLUS/GINSENG ROOT FERMENT FILTRATE CFDA: 乳酸杆菌 / 人参根发酵产物滤液 Concept : AntiAging & Brightening 2
3 Contents Bio A. Gold Ginseng B. Bioconversion C. AntiAging D. Brightening 3
4 Bio A. Gold Ginseng Rare type of Korean ginseng first discovered in 長湍郡, 京畿道, Korea Gold berries on the top differentiated from regular ginseng in appearance. Compared to regular ginseng, gold ginseng better maintains a good quality as it has no inside cavities and whites. Gold ginsengs are richer in saponin (ginsenoside) which have great medical effects. 4
5 Bio Contents Ginsenoside (Ginseng Saponin) Acidicpolysaccharide Polyacetylene Phenolic Acid Phytosterols and Essential Oils Proteins and Peptides Amino Acids Alkaloids Minerals Lignans A. Gold Ginseng Saponin of Ginseng, Ginsenoside Saponin is a bioactive substance known to improve the body immune system. However, certain saponins from certain vegetables carry toxicity and may generate a side effect such as hemolysis if taken much. On the other hand Saponin of ginseng, ginsenoside (ginseng + glycoside), is a neutral glycoside, and comes with no toxicity issues and has excellent medical effects. 5
6 Bio A. Gold Ginseng Contents of Ginsenoside Our recent research shows that ginsenosides are found in the root producing a stronger biological activity. Rb1 Rb2 Rc Rd Re Rf Rg1 Rg2 Root Leaf > Root (Ginsenosides) Stem 0.1> 0.1> 0.1> > Fruit
7 Bio A. Gold Ginseng Types of Ginsenoside 80% of ginsenosides found in the natural form of gold ginsengs are the major ginsenosides (Rb1, Rb2, Rc, Rd, Re, Rg1). R 1 O R 3 O HO HO R 3 O HO R 2 R 1 O COOR Protopanaxadiol Ra1, Ra2, Ra3, Rb1, Rb2, Rb3, Rc, Rd Rg3, Rh2, Compound K and so on Re, Rg1, R1, Rf Protopanaxatrio Rg2, Rh1, F1 and so on Oleanane Type Ro 7
8 Contents Bio A. Gold Ginseng B. Bioconversion C. AntiAging D. Brightening 8
9 Bio B. Bioconversion Rather than major ginsenosides, minor ginsenosides are known to be a better option for the topical usage. Therefore there were many attempts to convert from major ginsenosides to minor ginsenosides throughout to achieve the enhanced performance. 9
10 Bio Chemical method Physical method B. Bioconversion Biological method 1. Microbial transformation 2. Enzymatic transformation Chemical method: Poor selection, generation of side reactions and emission of environmental pollution. Physical method: Economic reason and low ginsenoside content. Biological method: Excellent selection, yield and productivity. It is a useful tool for structural modification and metabolism studies (Ko et al. 2007) (Ex. human intestinal bacteria, microorganisms from soil and fermented foods) 10
11 Bio B. Bioconversion Process of Bioconversion allowed us to convert major ginsenosides to minor ginsenosides and increased the concentration of compounds such as Compound K, Rh1, Rh2, Rg5, Rk1, PPD and PPT with enhanced biological activities. 11
12 Newly Discovered Microorganism Bio Change in Saponin (TLC & HPLC) Gold Ginseng Extract Bio B. Bioconversion Increase in minor ginsenosides Currently studying change in Saponin by using a newly discovered strain: Lactobacillus pentosus GFC01. 12
13 Bio B. Bioconversion Active Compound Content Analysis of Bio ppm General ginseng BioGold Active compounds <Gold Ginseng Extract> <BioGoldgiseng> CK, F2 and Rg2 are internationally recognized antiwrinkle ingredients. BioGoldinseng s excellent functionality derives from 9 times higher contents of actives than gold ginseng extract. SCI 2014: Enzymemodified Panax ginseng inhibits UVBinduced skin aging through the regulation of procollagen type I and MMP1 expression SCI 2012: Fermenting Red Ginseng Enhances Its Safety and Efficacy as a Novel Skin Care AntiAging Ingredient:In Vitroand Animal Study 13
14 B. Bioconversion Structural Identification Bio 14
15 Contents Bio A. Gold Ginseng B. Bioconversion C. AntiAging D. Brightening 15
16 Bio C. AntiAging Cell Toxicity No cell toxicity has confirmed. 발효전 Before Fermentation 발효 After 후 Fermentation 16
17 Bio The patch test was conducted on the 33 subjects ID Blank Bio Patch Test Leaf Stem 0.1> 0.1> Fruit C. AntiAging Patch Test No irritation has found in all subjects. 17
18 Bio C. AntiAging Increased Efficacy via Bioconversion (MMP1 Inhibition, in vitro) GFC Co., Ltd. Inhibition of MMP1 Bio Gold Ginseng Extract GAPDH Increased3fold Gold ginseng extract Bio Figure. Inhibitory Effects of Extract and Bio on UV Induced MMP1 in HFF. 18
19 C. AntiAging Increased Efficacy via Bioconversion (Collagen Synthesis, in vitro) Bio Procollagen Gold Ginseng Extract Bio * human Foreskin Fibroblast (hff) cells were used Bio shows 58% higher collagen biosynthesis level than the gold ginseng extract. 19
20 C. AntiAging Increased Efficacy via Bioconversion (AntiWrinkle Test, in vivo) Bio Anti wrinkles trials 0 week 2 weeks 3 weeks 4 weeks Clinical test data of Bio shows a superior wrinkle inhibitory activity. 20
21 Contents Bio A. Gold Ginseng B. Bioconversion C. AntiAging D. Brightening 21
22 D. Brightening Increased Efficacy via Bioconversion Bio Compound : Normal ginseng ext. Vs. GFC BioGold(ginseng) MELANIN CONTENT ASSAY CELL LINE : B16BL6 Figure. Inhibitory Effects of Normal ginseng and GFC BioGold(ginseng) on αmshinduced Melanogenesis in B16 Cells. 22
23 D. Brightening Increased Efficacy via Bioconversion Bio Compound : Normal ginseng ext. Vs. GFC BioGold(ginseng) TYROSINASE ACTIVITY ASSAY CELL LINE : B16BL6 Figure. The inhibitory effect of Gold Ginseng Extract and Bio on cellular tyrosinase activity. 23
24 D. Brightening Increased Efficacy via Bioconversion Compound : Normal ginseng ext. Vs. GFC BioGold(ginseng) Effect of Re on mrna expression of melanogenesis related genes CELL LINE : B16BL6 Bio 24
25 Summary Bio Rare type of gold colored Korean ginseng was used for the studies. Isolated Lactobacillus sp. Nov. was used in order to convert major ginsenosides to minor ginsenosides resulting in better functionalities. Converted minor ginsenosides were confirmed to have great Anti Aging and Brightening effects. 25
26 Bio Thank You 26
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