OIE Reference Laboratory Reports Activities
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1 OIE Reference Laboratory Reports Activities Activities in 2014 This report h been submitted : :32:19 Name of disee (or topic) for which you are a designated OIE Reference Laboratory: Swine influenza Address of laboratory: Via Bianchi Brescia ITALY Tel.: +39 (0) Fax: +39 (0) address: emanuela.foni@izsler.it Website: Name (including Title) of Head of Laboratory (Responsible Official): Prof Stefano Cinotti Name (including Title Position) of OIE Reference Expert: Dr. Emanuela Foni DVM Veterinary Manager Which of the following defines your laboratory? Check all that apply: Governmental OIE Reference Laboratory Reports Activities,
2 ToR 1: To use, promote disseminate diagnostic methods validated according to OIE Stards 1. Did your laboratory perform diagnostic tests for the specified disee/topic for purposes such disee diagnosis, screening of animals for export, surveillance, etc.? (t for quality control, proficiency testing or staff training) Diagnostic Test Indicated in OIE Manual (/) Total number of test performed lt year Indirect diagnostic tests Nationally Internationally Haemagglutination inhibition test yes Direct diagnostic tests Nationally Internationally PCR yes Egg inoculation yes Cell Culture inoculation yes ToR 2: To develop reference material in accordance with OIE requirements, implement promote the application of OIE Stards. To store distribute to national laboratories biological reference products any other reagents used in the diagnosis control of the designated pathogens or disee. 2. Did your laboratory produce or supply imported stard reference reagents officially recognised by the OIE? 3. Did your laboratory supply stard reference reagents (non OIE-approved) /or other diagnostic reagents to OIE Member Countries? 2 OIE Reference Laboratory Reports Activities, 2014
3 Type of reagent available Related diagnostic test Produced/ provide Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg). of recipient OIE Member Countries Region of recipients Virus A/sw/It/437/76( H1N1)aSW/It/141/81 H1N1 To BIOBANK of Veterinary Resources OIE Collaboratin Centre 2 ml/ each virus 1 Virus:H1N1 A/sw/It/311368/2013 H3N2: A/sw/It/311349/2013 H1N2: A/sw/It/284922/2009 Haemoagglutination Inhibition test 10ml /each virus 1 Sera:H1N1: A/sw/It/311368/2013 H3N2: A/sw/It/311349/2013 H1N2: A/sw/It/284922/2009 a Haemoagglutination Inhibition test 20ml/each serum 1 Virus H1N1 A/sw/It/ /2013 To BIOBANK of Veterinary Resources OIE Collaborating Centre 22ml Virus H1N2 A/sw/It/346150/2013 to BIOBANK of Veterinary Resources OIE Collaborating Centre 22ml OIE Reference Laboratory Reports Activities,
4 Virus H3N2 A/sw/It/282811/2013 to BIOBANK of Veterinary Resources OIE Collaborating Centre 22ml 4. Did your laboratory produce vaccines? 5. Did your laboratory supply vaccines to OIE Member Countries? ToR 3: To develop, stardise validate, according to OIE Stards, new procedures for diagnosis control of the designated pathogens or disees 6. Did your laboratory develop new diagnostic methods validated according to OIE Stards for the designated pathogen or disee? 7. Did your laboratory develop new vaccines according to OIE Stards for the designated pathogen or disee? ToR 4: To provide diagnostic testing facilities,, where appropriate, scientific technical advice on disee control meures to OIE Member Countries 8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? 4 OIE Reference Laboratory Reports Activities, 2014
5 Name of OIE Member Country seeking sistance Date (month). samples received for provision of diagnostic support. samples received for provision of confirmatory diagnoses SPAIN January 14 SPAIN April 100 BULGARIA vember 70 ROMANIA October 60 UKRAINE January 58 HUNGARY January 12 HUNGARY April 45 HUNGARY June 101 HUNGARY December 86 RUSSIA May 5 9. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? Name of the OIE Member Country receiving a technical consultancy Purpose How the advice w provided FRANCE Screening tests to characterize a pig herd free from Flu ITALY Real Time PCR for gene M Influenza A virus ITALY Sequences philogenetic analysis of H3N2 Swine influenza virus isolated from human in loco ToR 5: To carry out /or coordinate scientific technical studies in collaboration with other laboratories, centres or organisations 10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? OIE Reference Laboratory Reports Activities,
6 Title of the study Duration Purpose of the study Partners (Institutions) CoVetLab:Development validation of molecular tools for sub-typing swine influenza viruses one year, now extended to May 2015 Development validation of improved real-time reverse transcription polymere chain reaction (RRT-PCR) says for differentiation of swine influenza virus (SIV) sub-types H1N1, H1N2 H3N2 in an pigs the variant rth an H3N2 sub-type. DTU (Denmark), ANSES (France), CVI- Lelystad (The Netherls), SVA (Sweden) AHVLA (United Kingdom) ToR 6: To collect, process, analyse, publish disseminate epizootiological data relevant to the designated pathogens or disees 11. Did your Laboratory collect epizootiological data relevant to international disee control? 12. Did your laboratory disseminate epizootiological data that had been processed analysed? 13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: 3 -De Marco MA, Valentini A, Foni E, Savarese MC, Cotti C, Chiapponi C, Raffini E, Donatelli I, Delogu M. Is there a relation between genetic or social groups of mallard ducks the circulation of low pathogenic avian influenza viruses? Veterinary Microbiology 170 (2014) doi: /j.vetmic Chiapponi C., Baioni L., Luppi A., Moreno A., Ctellan A. Foni E. Temporal insight into the natural generation of a new resortant porcine influenza virus in a swine holding. Vet. Microbiol. (2014), - Simon G., Larsen L.E., Dürrwald R., Foni E., Harder T., Van Reeth K., Markowska-Daniel I., Reid S.M., Dan A., Maldonado J., Huovilainen A., Billinis C., Davidson I., Agüero M., Vila T., Hervé S., Breum S.Ø., Chiapponi C., Urbaniak K., Kyriakis C.S., ESNIP3 consortium, Brown I.H., Loeffen W. (2014) an surveillance network for influenza in pigs: surveillance programs, diagnostic tools swine influenza virus subtypes identified in 14 an countries from 2010 to PLoS One, 9(12):e doi: /journal.pone b) International conferences: 5 -Foni E., Chiapponi C., Moreno A., Baioni L., Luppi A., Faccini S. Swine influenza virus surveillance in Italian pig farms: Proceedings of 6th an Symposium of Porcine Health Management Sorrento Italy 7-9 May 2014, Arioli E., Caleffi A., Leotti G., Ostanello F., Foni E., Vila T., Joisel F. Booster effect of swine influenza virus infection on serological response to gripovac 3 vaccine in an Italian fattening unit Proceedings of 6th an Symposium of Porcine Health Management Sorrento Italy 7-9 May 2014, Leotti G., Foni E., Arioli E., Bongiovanni E., Bresaola M., Codato A., Donna R., Faccenda M., Fruttero F., Gamba F., Giorgiutti M., Salvini F., Zizioli B., Ostanello F., Merdy O.,Vila T. Joisel F. Serological responses in 11 Italian herds after vaccination with a combined vaccine against H1N1, H3N2 H1N2 Swine Influenza virus. Proceedings of the 23rd International Pig Veterinary Society 6 OIE Reference Laboratory Reports Activities, 2014
7 (IPVS) Congress June 8-11, 2014, Cancun, Mexico, 318. Swine influenza - Emanuela Foni - italy - Chiapponi C., Moreno A., Baioni L., Luppi A., Faccini S. Foni E Genetic characterization of Italian swine influenza viruses: Proceedings of the 23rd International Pig Veterinary Society (IPVS) Congress June 8-11, 2014, Cancun, Mexico, Antonio Piralla, Ana Moreno, Maria Ester Orli, Elena Percivalle, Chiara Chiapponi, Emanuela Foni, Mario Luini, Fausto Baldanti. Swine InfluenzaH3N2Virus infection in an immunocompromised adult patient, Italy th Annual Meeting of an Society for Clinical Virology. Prague 28/9-1/ c) National conferences: 1 Foni E., Arioli E., Bongiovanni E., Bresaola M., Codato A., Donna R., Faccenda M., Fruttero F., Gamba F., Giorgiutti M., Salvini F., Zizioli B., Leotti G., Merdy O., Vila T., Joisel F., Ostanello F. Influenza Suina: profili sierologici in animali vaccinati di allevamenti del rd Italia. Atti XL Meeting Annuale SIPAS Montichiari(BS) Marzo 2014, d) Other: (Provide website address or link to appropriate information) 0 ToR 7: To provide scientific technical training for personnel from OIE Member Countries To recommend the prescribed alternative tests or vaccines OIE Stards 14. Did your laboratory provide scientific technical training to laboratory personnel from other OIE Member Countries? ToR 8: To maintain a system of quality surance, biosafety biosecurity relevant for the pathogen the disee concerned 15. Does your laboratory have a Quality Management System certified according to an International Stard? Quality management system adopted EN ISO/IEC 17025:2005 Certificate scan (PDF, JPG, PNG format) Scan-Parma pdf 16. Is your laboratory accredited by an international accreditation body? 17. Does your laboratory maintain a biorisk management system for the pathogen the disee concerned? (See Manual of Diagnostic Tests Vaccines for Terrestrial Animals 2014, Chapter 1.1.3a) OIE Reference Laboratory Reports Activities,
8 ToR 9: To organise participate in scientific meetings on behalf of the OIE 18. Did your laboratory organise scientific meetings on behalf of the OIE? 19. Did your laboratory participate in scientific meetings on behalf of the OIE? Title of event Date (mm/yy) Location Role (speaker, presenting poster, short communications) Title of the work presented Third Global Conference of OIE Reference Centres October 2014 Incheon (Seoul) partecipant ToR 10: To establish maintain a network with other OIE Reference Laboratories designated for the same pathogen or disee organise regular inter-laboratory proficiency testing to ensure comparability of results 20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disee? 21. W your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disee by organising or participating in proficiency tests? Purpose of the proficiency tests: 1 Role of your Reference Laboratory (organiser/ participant). participants Participating OIE Ref. Labs/ organising OIE Ref. Lab. Polymere Chain Reaction for gene M partecipant 17 OIE Reference Laboratory for Avian Influenza. IZSVE Padua 1 validation of a diagnostic protocol: specify the test; quality control of vaccines: specify the vaccine type, etc. 22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disee on scientific research projects for the diagnosis or control of the pathogen of interest? 8 OIE Reference Laboratory Reports Activities, 2014
9 Title of the project or contract Scope Name(s) of relevant OIE Reference Laboratories CoVetLab Development validation of molecular tools for sub-typing swine influenza viruses AHVLA OIE Reference laboratory for Swine Influenza ToR 11: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens disees to ensure equivalence of results 23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disee? te: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: see point 1.3 ToR 12: To place expert consultants at the disposal of the OIE 24. Did your laboratory place expert consultants at the disposal of the OIE? Kind of consultancy Location Subject (facultative) Review of Chapter Swine Influenza in Manual of Diagnostic Tests Vaccines for Terrestrial Animals 2014 remote Chapter of Manual of Diagnostic Tests Vaccines for Terrestrial Animals Additional comments regarding your report: OIE Reference Laboratory Reports Activities,
OIE Reference Laboratory Reports Activities
OIE Reference Laboratory Reports Activities Activities in 206 This report h been submitted : 207-0-2 0:27:9 Name of disee (or topic) for which you are a designated OIE Reference Laboratory: Swine influenza
OIE Reference Laboratory Reports Activities
OIE Reference Laboratory Reports Activities Activities in 2016 This report has been submitted : 2017-01-31 14:46:00 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Newcastle
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OIE Reference Laboratory Reports Activities Activities in 2017 This report has been submitted : 2018-01-09 09:49:33 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Classical
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OIE Reference Laboratory Reports Activities Activities in 2013 This report has been submitted : 2014-01-31 12:34:26 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Newcastle
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OIE Reference Laboratory Reports Activities Activities in 2014 This report has been submitted : 2015-01-16 21:46:52 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: African
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OIE Reference Laboratory Reports Activities Activities in 2013 This report has been submitted : 2014-01-31 21:38:48 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: n
OIE Reference Laboratory Reports Activities
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OIE Reference Laboratory Reports Activities Activities in 2015 This report has been submitted : 2016-03-08 23:26:29 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Transmissible
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OIE Reference Laboratory Reports Activities Activities in 2015 This report has been submitted : 2016-01-18 11:23:08 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Equine
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OIE Reference Laboratory Reports Activities
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OIE Reference Laboratory Reports Activities
OIE Reference Laboratory Reports Activities Activities in 2017 This report has been submitted : 2017-12-21 15:32:58 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Classical
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