OIE Reference Laboratory Reports Activities

Transcription

1 OIE Reference Laboratory Reports Activities Activities in 2016 This report has been submitted : :57:12 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Salmonellosis Address of laboratory: APHA Weybridge Woodham Lane New Haw, Addlestone Surrey KT15 3NB UNITED KINGDOM Tel.: Fax: address: rob.davies@apha.gsi.gov.uk Website: Name (including Title) of Head of Laboratory (Responsible Official): Dr. Kath Webster Name (including Title and Position) of OIE Reference Expert: Dr. Rob Davies, Head of OIE Reference Laboratory Which of the following defines your laboratory? Check all that apply: Governmental OIE Reference Laboratory Reports Activities,

2 ToR 1: To use, promote and disseminate diagnostic methods validated according to OIE Standards 1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as disease diagnosis, screening of animals for export, surveillance, etc.? (t for quality control, proficiency testing or staff training) Diagnostic Test Indicated in OIE Manual (/) Total number of test performed last year Indirect diagnostic tests Nationally Internationally SAT (S. Gallinarum/Pullorum) yes SAT (S. Abortusequi) yes SAT (S. Dublin) yes 68 0 SAT (S. Typhimurium) yes 25 0 RSA (S. Gallinarum/Pullorum) yes Direct diagnostic tests Nationally Internationally Serotyping yes Phagetyping yes Antimicrobial susceptibility testing yes Tests for live Salmonella vaccines yes Salmonella culture yes ToR 2: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease. 2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE? 2 OIE Reference Laboratory Reports Activities, 2016

3 3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents to OIE Member Countries? Type of reagent available Related diagnostic test Produced/ provide Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg). of recipient OIE Member Countries Region of recipients Salmonella typing sera Serotyping 42,065 ml 22,857 ml 600 ml commercially sensitive information America s Asia and Pacific Middle East S. Pullorum control serum SAT none 13.5 ml 4 ml commercially sensitive information America s Asia and Pacific Middle East S. Pullorum antigen RSA and SAT 9,675 ml none 8,575 ml commercially sensitive information America s Asia and Pacific Middle East 4. Did your laboratory produce vaccines? 5. Did your laboratory supply vaccines to OIE Member Countries? ToR 3: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases 6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease? OIE Reference Laboratory Reports Activities,

4 7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? ToR 4: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries 8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? 9. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? Name of the OIE Member Country receiving a technical consultancy Purpose How the advice was provided PORTUGAL Advice on serological surveillance of pigs at the abattoir remote assistance SUDAN Scientific advice on function of flagella remote assistance BOSNIA AND HERZEGOVINA Advice on Salmonella control provision of learning material (DVDs) SAUDI ARABIA Advice on prevalence of S. Derby in poultry remote assistance CANADA Advice on best sampling options for chicken breeding and laying flocks remote assistance RUSSIA Advice on control of ST in hatching eggs remote assistance LITHUANIA Advice on control of Salmonella in a breeding facility of feeder mice remote assistance RUSSIA Advice on Salmonella prevention in feed mills remote assistance CHINA (PEOPLE'S REP. OF) Advice on requirements for OIE twinning and arrangement of reference laboratory visit in China remote assistance ToR 5: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations 10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? 4 OIE Reference Laboratory Reports Activities, 2016

5 Title of the study Duration Purpose of the study Partners (Institutions) OIE Member Countries involved other than your country Molecular analysis of ST DT 8 isolates from ducks 6 months Comparison of isolates at a molecular level ANSES, IZSVe,Dep for Agriculture Food and the Marine - Ireland FRANCE IRELAND ITALY Document concerning conventional serotyping vs WGS serotyping of Salmonella for the EC. 3 months document concerning conventional serotyping vs WGS serotyping of Salmonella. For the EC. EU-RL, Netherlands THE NETHERLANDS SE outbreak study 9 months Molecular analysis of outbreak strains Dep for Agriculture Food and the Marine, RIVM Netherlands IRELAND THE NETHERLANDS ToR 6: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases 11. Did your Laboratory collect epizootiological data relevant to international disease control? 12. Did your laboratory disseminate epizootiological data that had been processed and analysed? 13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: 18 Anjum, M.F., Duggett, N.A., AbuOun, M., Randall, L., Nunez-Garcia, J., Ellis, R.J., Rogers, J., Horton, R., Brena, C., Williamson, S., Martelli, F., Davies, R., Teale, C. (2016) Colistin resistance in Salmonella and Escherichia coli isolates from a pig farm in Great Britain. J Antimicrob Chemother. 71(8): doi: /jac/dkw149. Davies, R., Gosling, R. J., Wales, A.D., Smith, R.P. (2016) Use of an attenuated live Salmonella Typhimurium vaccine on three breeding pig units: a longitudinal observational field study. Comp Immunol Microbiol Infect Dis.46:7-15. Card, R., Vaughan, K., Bagnall, M. et al. (2016). Virulence Characterisation of Salmonella enterica Isolates of Differing Antimicrobial Resistance Recovered from UK Livestock and Imported Meat Samples. Frontiers in Microbiology 7: 640. Figueiredo, R., Card, R.M., Nunez, J. et al. (2016). Detection of an mcr-1-encoding plasmid mediating colistin resistance in Salmonella enterica from retail meat in Portugal. The Journal of Antimicrobial Chemotherapy 71 (8): Gosling, R.J., Breslin, M., Fenner, J., Vaughan, K., West, E., Mawhinney, I., Birch, C., Davies, R.H. (2016) An invitro investigation into the efficacy of disinfectants used in the duck industry against Salmonella. Avian Pathology OIE Reference Laboratory Reports Activities,

6 45, Hayward, M.R., Petrovska, L., Jansen, V.A., Woodward, M.J. (2016) Population structure and associated phenotypes of Salmonella enterica serovars Derby and Mbandaka overlap with host range. BMC Microbiol. 4;16:15. doi: /s Hill, A.A., Simons, R.R.L., Kelly, L. and Snary, E.L. (2016) A Farm Transmission Model for Salmonella in Pigs, Applicable to E.U. Member States. Risk Analysis, 36: doi: /risa Hill, A.A., Simons, R.L., Swart, A.N., Kelly, L., Hald, T. and Snary, E.L. (2016) Assessing the Effectiveness of On- Farm and Abattoir Interventions in Reducing Pig Meat Borne Salmonellosis within E.U. Member States. Risk Analysis, 36: doi: /risa Martelli, F., Birch, C. and Davies, R. Observations on the distribution and control of Salmonella in commercial duck hatcheries in the United Kingdom. Avian Pathology 45(2):261-6 doi / Martelli, F., Gosling, R.J., Callaby, R., Davies, R. (2016) Observation on Salmonella contamination of commercial duck farms before and after cleaning and disinfection. Avian Pathol. 22:1-19. Naqid, I.A., Owen, J.P., Maddison, B.C., Spiliotopoulos, A., Emes, R.D., Warry, A., Tchórzewska, M.A., Martelli, F., Gosling, R.J., Davies, R.H., La Ragione, R.M., Gough, K.C. (2016) Mapping polyclonal antibody responses to bacterial infection using next generation phage display. Sci Rep. 6: doi: /srep Naqid, I.A., Owen, J.P., Maddison, B.C., Spiliotopoulos, A., Emes, R.D., Warry, A., Flynn, R.J., Martelli, F., Gosling, R.J., Davies, R.H., La Ragione, R.M., Gough, K.C. (2016) Mapping B-cell responses to Salmonella enterica serovars Typhimurium and Enteritidis in chickens for the discrimination of infected from vaccinated animals. Sci Rep. 6: doi: /srep Petrovska, L., Mather, A.E., AbuOun, M., Branchu, P., Harris, S.R., Connor, T., Hopkins, K.L., Underwood, A., Lettini, A.A., Page, A., Bagnall, M., Wain, J., Parkhill, J., Dougan, G., Davies, R., Kingsley, R.A. (2016) Microevolution during the emergence of the monophasic Salmonella Typhimurium epidemic. Emerg Infect Dis. 22(4): doi: /eid Simons, R.R.L., Hill, A.A., Swart, A., Kelly, L. and Snary, E.L. (2016) A Transport and Lairage Model for Salmonella Transmission Between Pigs Applicable to EU Member States. Risk Analysis, 36: doi: /risa Snary, E. L., Swart, A. N., Simons, R. R. L., Domingues, A.R.C., Vigre, H., Evers, E. G., Hald, T. and Hill, A.A. (2016) A Quantitative Microbiological Risk Assessment for Salmonella in Pigs for the an Union. Risk Analysis, 36: doi: /risa Snary, E. L., Swart, A. N. and Hald, T. (2016) Quantitative Microbiological Risk Assessment and Source Attribution for Salmonella: Taking it Further. Risk Analysis, 36: doi: /risa Swart, A.N., Evers, E.G., Simons, R.L.L. and Swanenburg, M. (2016) Modeling of Salmonella Contamination in the Pig Slaughterhouse. Risk Analysis, 36: doi: /risa Vigre, H., Barfoed, K., Swart, A.N., Simons, R.R.L., Hill, A.A., Snary, E.L. and Hald, T. (2016) Characterization of the Human Risk of Salmonellosis Related to Consumption of Pork Products in Different EU Countries Based on a QMRA. Risk Analysis, 36: doi: /risa b) International conferences: 20 De Lucia, A., Rabie, A., Martelli, F., Smith, R.P., Davies, R. Persistence of Salmonella in the environment and role of wild birds in the epidemiology of infection in an outdoor pig farm in the United Kingdom. International Pig Veterinary Society conference 2016, Dublin Ireland 7-10 June Gosling, R.J., Breslin, M., Mawhinney, I., Davies, R.H. Efficacy of different disinfectants intended for a pig farm environment. IDT Woerlitzer Tierkolleg, Germany, September 2016 Gosling, R.J., Williamson, S., Breslin, M., Mawhinney, I., Davies, R.H. Efficacy of different disinfectants intended for a pig farm environment. 24th International Pig Veterinary Society Congress (IPVS), Dublin, Ireland, June 2016 Gosling, R.J., Breslin, M., Fenner, J., Vaughan, K., West, E., Mawhinney, I., Birch, C., Davies, R.H. An in-vitro investigation into the efficacy of disinfectants used in the duck industry against Salmonella. International Symposium Salmonella and Salmonellosis, Saint Malo, France 6-8 June Gosling, R.J., Street, P.F.S., Mawhinney, I., Davies, R.H. Improved control of Salmonella and pathogenic E. coli contamination of animal feed using a novel alternative to formaldehyde-based treatment. International Symposium Salmonella and Salmonellosis, Saint Malo, France 6-8 June Gosling, R.J. and Davies, R.H. Investigation of Salmonella carcass contamination in a high-throughput pig abattoir in Great Britain. International Symposium Salmonella and Salmonellosis, Saint Malo, France 6-8 June Gosling, R.J., Breslin, M., Mawhinney, I., Davies, R.H. Efficacy of different disinfectants intended for a pig farm environment. International Symposium Salmonella and Salmonellosis, Saint Malo, France 6-8 June Gosling, R., Mueller-Doblies, D., Breslin, M., Nunez-Garcia, J., Rabie, A., Wales, A. and Davies, R.H. Observations on the distribution and persistence of monophasic Salmonella Typhimurium on infected pig farms in Great Britain from 2010 to International Symposium Salmonella and Salmonellosis, Saint Malo, France 6-8 June Kingsley. R., Petrovska, L. Genotypic and phenotypic diversity arising during the clonal expansion of the current MDR monophasic Salmonella Typhimurium epidemic in the UK. International Symposium Salmonella and Salmonellosis, Saint Malo, France 6-8 June OIE Reference Laboratory Reports Activities, 2016

7 Martelli, F. and Davies. R. Salmonella in poultry: new and emerging serovars. Invited speaker at the International poultry conference in Tara, Serbia, on 27/09/2016. Martelli, F., Smith, R.P. and Davies, R.H. Observation on the introduction and dissemination of Salmonella in three previously low prevalence status pig farms in the United Kingdom. International Symposium Salmonella and Salmonellosis, Saint Malo, France 6-8 June Martelli, F., Smith, R.P. and Davies, R.H. Observation on the prevalence and distribution of Salmonella in ten outdoor pig farms in the United Kingdom. International Symposium Salmonella and Salmonellosis, Saint Malo, France 6-8 June Mueller-Doblies, D., Speed, K., and Davies, R.H. 32 years of Salmonella Typhimurium in livestock in Great Britain. International Symposium Salmonella and Salmonellosis, Saint Malo, France 6-8 June Petrovska, L., Martelli, F., Kell, N., Gosling, R., Tang, Y., De Pinna, E., Davies, R., Kingsley, R. Retrospective analysis of duck-related Salmonella Typhimurium isolates in the UK using whole genome sequencing. International Symposium Salmonella and Salmonellosis, Saint Malo, France 6-8 June Rabie, A., Martelli, F., Smith, R.P., Davies, R. A study of antimicrobial resistance patterns of Salmonella detected on pig farms in the United Kingdom. Proceedings of the International Symposium Salmonella and Salmonellosis, Saint Malo, France 6-8 June Rabie, A. and Davies, R.H., Rodents and their role as vectors in Salmonella transmission. International Symposium Salmonella and Salmonellosis, Saint Malo, France 6-8 June Randall, L.P., Ashton, P., Horton, R.A., Davies, R.H. and Mueller-Doblies, D. Comparing Salmonella Derby from pigs, turkeys and humans in the UK a molecular study to identify possible implications for public health. International Symposium Salmonella and Salmonellosis, Saint Malo, France 6-8 June Smith, R.P. Controlled study of the use of a Salmonella Typhimurium vaccine on English pig farms. IDT Woerlitzer Tierkolleg, Germany, September 2016 Tang, Y., Ashton, P., Slowie, R., Fenner, J., Dallman, T., Davies, R., Petrovska, L. Characterisation of the Salmonella Enteritidis outbreak isolates using whole genome sequencing analysis. International Symposium Salmonella and Salmonellosis, Saint Malo, France 6-8 June Vaughan, K., Gosling, R.J., Lambert, M., Rabié, A., Oastler, C., Davies, R. H. Salmonella in rodents on pig farms before and after cleaning and disinfection. International Symposium Salmonella and Salmonellosis, Saint Malo, France 6-8 June c) National conferences: 8 Card, R. Modelling virulence and transmission of antimicrobial resistance in enteric pathogens from livestock. 19th Conference in Genomics and Proteomics of Human Pathogens, PHE Collindale, UK, June Davies, R., Martelli, F. Salmonella control and its relevance to biosecurity, farm hygiene and antibiotic usage. Speaker at the National Pig Association regional autumn meetings 2016 Exeter (04/10/16) and Newbury (10/10/16). Gosling, R.J., Breslin, M., Mawhinney, I., Davies, R.H. Efficacy of different disinfectants intended for a pig farm environment. British Pig and Poultry Fair poster, May 2016 Martelli, F., Gosling, R.J., Rodgers, J., Breslin, M., Davies, R.H. Efficacy of different disinfectants intended for a pig farm environment. Pig Health and Welfare council food safety sub group meeting 2016 Mueller-Doblies, D. An update on the Salmonella situation in poultry. rfolk Suffolk Poultry Association Meeting, October 2016 Mueller-Doblies, D. Salmonella in Poultry in the UK. Campden BRI Meat and Poultry Member Interest Group Meeting, January 2016 Petrovska, L. Application of NGS to Epidemiology and Outbreak Investigation of Salmonella in GB Livestock. 19th Conference in Genomics and Proteomics of Human Pathogens, PHE Collindale, UK, June Smith, R.P. Designing a cost-effective toolkit for Salmonella control. Pig Veterinary Society Spring Meeting d) Other: (Provide website address or link to appropriate information) 5 Martelli, F., Wales, A. and Davies, R. Of Mice and Hens, Tackling Salmonella in Table Egg Production in the United Kingdom and. In Producing Safe Eggs, Academic press, Elsevier. ISBN Wales, A. and Davies, R.H. Update of EFSA factsheets on S. Gallinarum, S. Pullorum and S. arizonae Davies. R.H. Update of the OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, eighth edition: Chapter on Fowl typhoid and Pullorum disease Contribution to the Update of the OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, eighth edition: Chapter on Salmonellosis Wyllie, S. Management of the Salmonella risk in feed in United Kingdom. EUROFAC / FEFAC Workshop on Salmonella risk management in feed. December 2016 OIE Reference Laboratory Reports Activities,

8 ToR 7: To provide scientific and technical training for personnel from OIE Member Countries To recommend the prescribed and alternative tests or vaccines as OIE Standards 14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries? a) Technical visits: 2 b) Seminars: 1 c) Hands-on training courses: 0 d) Internships (>1 month): 1 Type of technical training provided (a, b, c or d) Country of origin of the expert(s) provided with training. participants from the corresponding country a Thailand 4 a Thailand 7 d Italy 1 b worldwide (webinar) 20 ToR 8: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned 15. Does your laboratory have a Quality Management System certified according to an International Standard? Quality management system adopted ISO GLP ISO9001 Certificate scan (PDF, JPG, PNG format) UKAS Certificate (2).pdf GLP Certificate pdf 9001 Certificate front cover.pdf 16. Is your laboratory accredited by an international accreditation body? 8 OIE Reference Laboratory Reports Activities, 2016

9 Test for which your laboratory is accredited Various Salmonella surveillance and diagnostic tests Various serological and AMR tests Various research methodologies Accreditation body UKAS UKAS Lloyds Register 17. Does your laboratory maintain a biorisk management system for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Chapter 1.1.4) ToR 9: To organise and participate in scientific meetings on behalf of the OIE 18. Did your laboratory organise scientific meetings on behalf of the OIE? 19. Did your laboratory participate in scientific meetings on behalf of the OIE? ToR 10: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results 20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease? 21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests? OIE Reference Laboratory Reports Activities,

10 Purpose of the proficiency tests: 1 Role of your Reference Laboratory (organiser/ participant). participants Participating OIE Ref. Labs/ organising OIE Ref. Lab. Assess laboratory capability to conduct isolation and identification of Salmonella species to help maintain accreditation. Animal and Plant Health Agency Vetqas PT scheme PT0087 Salmonella in Animal Feed participant 14 UK Assess laboratory capability to conduct isolation and identification of Salmonella species to help maintain accreditation. Animal and Plant Health Agency Vetqas PT scheme PT0088 Salmonella in Poultry participant 92 UK, Canada, Italy, Germany Assess laboratory capability to conduct isolation and identification of Salmonella species to help maintain accreditation. Animal and Plant Health Agency Vetqas PT scheme PT0090 Control of Salmonella in Poultry Order (run for Defra for UK labs only) participant 24 UK Assess laboratory capability to conduct isolation and identification of Salmonella species to help maintain accreditation. Animal and Plant Health Agency Vetqas PT scheme PT0084 Salmonella serotyping and culture participant 27 UK, Germany 1 validation of a diagnostic protocol: specify the test; quality control of vaccines: specify the vaccine type, etc. 22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest? Title of the project or contract Scope Name(s) of relevant OIE Reference Laboratories ENGAGE an Project to maximise use of WGS applications IZSVe Italy ToR 11: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results 23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease? 10 OIE Reference Laboratory Reports Activities, 2016

11 te: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: see point 1.3 Purpose for inter-laboratory test comparisons 1. participating laboratories Region(s) of participating OIE Member Countries Assess laboratory capability to conduct isolation and identification of Salmonella species to help maintain accreditation. Animal and Plant Health Agency Vetqas PT scheme PT0087 Salmonella in Animal Feed 14 Americas Asia and Pacific Middle East Assess laboratory capability to conduct isolation and identification of Salmonella species to help maintain accreditation. Animal and Plant Health Agency Vetqas PT scheme PT0088 Salmonella in Poultry 92 Americas Asia and Pacific Middle East Assess laboratory capability to conduct isolation and identification of Salmonella species to help maintain accreditation. Animal and Plant Health Agency Vetqas PT scheme PT0090 Control of Salmonella in Poultry Order (run for Defra for UK labs only) 24 Americas Asia and Pacific Middle East Assess laboratory capability to conduct isolation and identification of Salmonella species to help maintain accreditation. Animal and Plant Health Agency Vetqas PT scheme PT0084 Salmonella serotyping and culture 27 Americas Asia and Pacific Middle East Salmonella detection and serotyping EU-RL ringtrials 36 Americas Asia and Pacific Middle East ToR 12: To place expert consultants at the disposal of the OIE 24. Did your laboratory place expert consultants at the disposal of the OIE? OIE Reference Laboratory Reports Activities,

12 Kind of consultancy Location Subject (facultative) Update of the OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, eighth edition: Chapter on Fowl typhoid and Pullorum disease correspondence Diagnosis and Control Contribution to the Update of the OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, eighth edition: Chapter on Salmonellosis correspondence Diagnosis and Control 25. Additional comments regarding your report: This report was compiled by Doris Mueller-Doblies on behalf of Robert Davies. 12 OIE Reference Laboratory Reports Activities, 2016