OIE Reference Laboratory Reports Activities

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1 OIE Reference Laboratory Reports Activities Activities in 2016 This report has been submitted : :04:59 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: New world screwworm (Cochliomyia hominivorax) Address of laboratory: Apartado Postal Panama PANAMA Tel.: Fax: address: john.b.welch@aphis.usda.gov Website: Name (including Title) of Head of Laboratory (Responsible Official): Francisco Pinilla, General Director for Panama, Panama - United States Commission for the Eradication and Prevention of Screwworm (COPEG); Antonio Arroyave, General Director for the United States, COPEG Name (including Title and Position) of OIE Reference Expert: John B. Welch, USDA-APHIS-IS Entomologist Which of the following defines your laboratory? Check all that apply: Other: Binational Commission OIE Reference Laboratory Reports Activities,

2 ToR 1: To use, promote and disseminate diagnostic methods validated according to OIE Standards 1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as disease diagnosis, screening of animals for export, surveillance, etc.? (t for quality control, proficiency testing or staff training) Diagnostic Test Indicated in OIE Manual (/) Total number of test performed last year Indirect diagnostic tests Nationally Internationally 0 0 Direct diagnostic tests Nationally Internationally Microscopic dissection of larval samples 163 (67 positive) Visual inspection of animals for screwworm infestation at inspection stations 122,205 (0 positive) ToR 2: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease. 2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE? 3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents to OIE Member Countries? 4. Did your laboratory produce vaccines? 5. Did your laboratory supply vaccines to OIE Member Countries? 2 OIE Reference Laboratory Reports Activities, 2016

3 ToR 3: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases 6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease? 7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? ToR 4: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries 8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? 9. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? Name of the OIE Member Country receiving a technical consultancy Purpose How the advice was provided UNITED STATES OF AMERICA Eradication of a screwworm outbreak in Florida Leadership in the field in Florida, telephone conversations, personal conversations, s, photos, and videos. PERU Screwworm rearing and mating compatibility testing. Telephone conversations and s BRAZIL Protocol for collecting screwworm larval samples for DNA characterization. s ToR 5: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations 10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? OIE Reference Laboratory Reports Activities,

4 Title of the study Duration Purpose of the study Partners (Institutions) OIE Member Countries involved other than your country Development of a Transgenic Male-Only Strain of New World Screwworm for Mass Production and Release 1 Year Develop additional maleonly strains to reduce costs of mass production, increase efficiency of eradication and make screwworm eradication more economical for use in other countries. rth Carolina State University UNITED STATES OF AMERICA ToR 6: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases 11. Did your Laboratory collect epizootiological data relevant to international disease control? 12. Did your laboratory disseminate epizootiological data that had been processed and analysed? 13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: 5 Sagel, A., P. Phillips, M.Chaudhury and S.R. Skoda Managing ammonia emissions from screwworm larval rearing media. J. Econ. Entomol. 109: Chaudhury, M.F., J.J. Zhu and S.R. Skoda Bacterial volatiles attract gravid secondary screwworms (Diptera: Calliphoridae). J. Econ. Entomol. 109: Concha, C., A. Palavesam, F.D. Guerrero, A. Sagel, F. Li, J.A. Osborne, Y. Hernandez, T, Pardo, G. Quintero, M. Vasquez, G.P. Keller, P.L. Phillips, J.B. Welch, W.O. McMillan, S.R. Skoda and M.J. Scott A transgenic maleonly strain of the New World screwworm for an improved control program using the sterile insect technique. BMC Biology 14:72 DOI /s Skoda, S. R., P. L. Phillips, A. Sagel, and M. F. Chaudhury. Distribution and persistence of sterile screwworms released at the Panama-Colombia border. J. Econ. Entomol. (accepted Dec. 2016). Chaudhury, M. F., J. J. Zhu, and S. R. Skoda. Physical and physiological factors influence behavioral responses of screwworm flies to synthetic attractants. J. Econ. Entomol. (accepted Dec. 2016). b) International conferences: 2 IAEA RLA5067/002 Regional Training Course of Natural Barriers as Methods of Control for Screwworm in Juazeiro, Brazil, January 2016, "Influencia de los Ríos y Otras Barreras Naturales de la Dispersión y el Control del Gusano Barrenador del Ganado," by Pamela L. Phillips. IAEA RLA/5/067 Project evaluation and coordination meeting of the Technical Cooperation project Supporting Capacity Building for Evaluation of Feasibility of a Progressive Control Programme for New World Screwworm," in Vienna, Austria, December 2016, Operaciones de Campo para el Brote del GBG, Cayos de Florida 2016," by 4 OIE Reference Laboratory Reports Activities, 2016

5 Pamela L. Phillips. c) National conferences: 2 United States Animal Health Association (USAHA) annual meeting, Greensboro, rth Carolina, October 2016, "Research Update on Screwworms," by Steve Skoda, presented in absentia by Robert Miller. Congreso Panamericano de Medicina Veterinaria, Panama, Panama, October 2016,"Analisis de Riesgo en Vigilancia Epidemiologica de Enfermedades Parasitarias - GBG," presented by Janina Subia. d) Other: (Provide website address or link to appropriate information) 8 Informed Ministry of Agricultural Development and the information branch of the OIE of positive cases in Panama. Educational Educational presentations on wound treatment and sterile fly production at 10 livestock fairs in the provinces of Panama, January - December Training of 546 Honorary Inspectors throughout Panama, but concentrating in the Barrier Zone, January - December Presented 107 lectures throughout Panama to 3,288 Livestock producers in animal husbandry techniques with emphasis on prevention of screwworm and other trans-boundary diseases, January - December Distributed 12, COPEG calendars to livestock producers and others throughout Panama. Presented 160 certificates of appreciation to those individuals who collected and submitted myiasis larval samples for identification. Radio info-spots on Emisora Voz son Frontera, in the Darien Province; and Radio Panama, throughout Panama, January - December Provided education and training in screwworm biology, ecology, and eradication to personnel of the USDA-APHIS- VS, USDA-APHIS-WS, USDA-APHIS-PPQ, US Department of the Interior; Florida Department of Agriculture and Consumer Services; Monroe County Mosquito Control District and the Boy Scouts of America; Florida, United States of America, October - December. ToR 7: To provide scientific and technical training for personnel from OIE Member Countries To recommend the prescribed and alternative tests or vaccines as OIE Standards 14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries? a) Technical visits: 1 b) Seminars: 0 c) Hands-on training courses: 3 d) Internships (>1 month): 3 OIE Reference Laboratory Reports Activities,

6 Type of technical training provided (a, b, c or d) Country of origin of the expert(s) provided with training. participants from the corresponding country a USA 5 b c USA 30 d Panama 5 ToR 8: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned 15. Does your laboratory have a Quality Management System certified according to an International Standard? Explain Quality Management System in adoption process or currently in place In process of ISO 9001:2008 certification. 16. Is your laboratory accredited by an international accreditation body? 17. Does your laboratory maintain a biorisk management system for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Chapter 1.1.4) ToR 9: To organise and participate in scientific meetings on behalf of the OIE 18. Did your laboratory organise scientific meetings on behalf of the OIE? 19. Did your laboratory participate in scientific meetings on behalf of the OIE? 6 OIE Reference Laboratory Reports Activities, 2016

7 ToR 10: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results 20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease? t applicable (Only OIE Reference Lab. designated for disease) 21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests? t applicable (Only OIE Reference Lab. designated for disease) 22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest? t applicable (Only OIE Reference Lab. designated for disease) ToR 11: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results 23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease? te: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: see point 1.3 ToR 12: To place expert consultants at the disposal of the OIE 24. Did your laboratory place expert consultants at the disposal of the OIE? 25. Additional comments regarding your report: OIE Reference Laboratory Reports Activities,

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