OIE Reference Laboratory Reports Activities
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1 OIE Reference aboratory Reports Activities Activities in 2016 This report has been submitted : :18:25 Name of disease (or topic) for which you are a designated OIE Reference aboratory: Equine influenza Address of laboratory: Irish Equine Centre Johnstown Naas Co. Kildare IREAND Tel.: Fax: address: acullinane@irishequinecentre.ie Website: Name (including Title) of Head of aboratory (Responsible Official): Sarah McNicholas BBS MSC Chief Executive Officer Name (including Title and Position) of OIE Reference Expert: Professor Ann Cullinane Head of Virology Which of the following defines your laboratory? Check all that apply: Other: Registered Charity OIE Reference aboratory Reports Activities,
2 ToR 1: To use, promote and disseminate diagnostic methods validated according to OIE Standards 1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as disease diagnosis, screening of animals for export, surveillance, etc.? (Not for quality control, proficiency testing or staff training) Diagnostic Test Indicated in OIE Manual (/No) Total number of test performed last year Indirect diagnostic tests Nationally Internationally Single radial haemolysis Haemagglutination Inhibition Direct diagnostic tests Nationally Internationally RT-PCR Virus Isolation 0 4 ToR 2: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease. 2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE? NOTE: Currently, there are 22 laboratories that produce Standard Reference Reagents officially recognised by the OIE for 19 diseases/pathogens. Please click the following link to the list of OIE-approved International Standard Sera: If the reagent is not listed on this page, it is NOT considered OIE-approved. The next two questions allow you to indicate non-oie-approved diagnostic reagents. 2 OIE Reference aboratory Reports Activities, 2016
3 Disease Test Available from Equine influenza Haemagglutination inhibition; single radial haemolysis Dr Marie-Emmanuelle Behr-Gross European Directorate for the Quality of Medicines, Council of Europe, 224 avenue de Colmar (entrée rue Schertz), F Strasbourg, France Tel: 33 (0) Fax: 33(0) OIE Reference aboratory Reports Activities,
4 Type of reagent available Related diagnostic test Produced/ Supply imported Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg) Name of recipient OIE Member Countries A/eq/2/Nemarket/2/93 POAND A/eq/2/Nemarket/2/93 SINGAPORE A/eq/South Africa/4/03 POAND A/eq/South Africa/4/03 URUGUAY A/eq/South Africa/4/03 SOUTH AFRICA A/eq/1/Newmarket/77 URUGUAY A/eq/1/Newmarket/77 SINGAPORE A/eq/2/Newmarket/93 SINGAPORE 3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents to OIE Member Countries? 4 OIE Reference aboratory Reports Activities, 2016
5 Type of reagent available Related diagnostic test Produced/ provide Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg) No. of recipient OIE Member Countries Region of recipients A/eq/Meath/07 H3N8 Clade 2 Florida sublineage SRH HI RT- PCR Produced 0 4ml 2 Africa America s Asia and Pacific Europe Middle East A/eq/Donegal/09 Clade 1 Florida sublineage SRH HI RT- PCR Produced 0 2ml 1 Africa America s Asia and Pacific Europe Middle East 4. Did your laboratory produce vaccines? No 5. Did your laboratory supply vaccines to OIE Member Countries? No ToR 3: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases 6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease? No 7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? No ToR 4: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries 8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? OIE Reference aboratory Reports Activities,
6 Name of OIE Member Country seeking assistance Date (month) No. samples received for provision of diagnostic support No. samples received for provision of confirmatory diagnoses MAAYSIA May 0 4 UNITED KINGDOM April 10 0 UNITED KINGDOM May 2 0 UNITED KINGDOM June 35 0 UNITED KINGDOM July 1 0 UNITED KINGDOM August 1 0 UNITED KINGDOM December 1 0 AUSTRIA April Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? Name of the OIE Member Country receiving a technical consultancy Purpose How the advice was provided MAAYSIA Revision of import requirements for racehorses Remote URUGUAY Performance of SRH In loco SINGAPORE Performance of SRH Remote FINAND Harmonisation of vaccination rules of European Trotting Union Remote GERMANY Vaccination of sport horses Remote ToR 5: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations 10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? 6 OIE Reference aboratory Reports Activities, 2016
7 Title of the study Duration Purpose of the study Partners (Institutions) OIE Member Countries involved other than your country Evaluation of detection of equine influenza virus H3N8 subtype and equine herpesviruses 1 and 4 by insulated isothermal RT-PCR (iirt-pcr) and PCR (iipcr) assays respectively using the POCKITTM Nucleic Acid Analyzer 1 week To determine if this technology was suitable to use on site at the Olympic Games and other international equestrian events Genereach KOREA (REP. OF) Evaluation of current equine influenza vaccination protocols prior to shipment, guided by the OIE Standards The aim of this study is to provide a science-based rationale to identify the ideal time period for vaccination prior to shipment, in support of the harmonisation of the FEI and IFHA recommendations with regard to equine influenza vaccination prior to shipment, guided by the OIE Standards. Japan Racing Association Instituto de Virología, CICVyA INTA, Hurlingham, Buenos Aires, Argentina Animal Health aboratory Department, Animal & Plant Health Center, Agri-Food & Veterinary Authority of Singapore SINGAPORE Validation study on real time RT-PCR diagnostic assay(s) for equine influenza in horses To validate an equine influenza real time RT-PCR assay(s) for equine influenza viral RNA detection in horses for the intended purpose of certifying freedom from infection in individual animals for trade or movement. Animal Health Trust, Newmarket, U.K. Gluck Equine Research Centre, Kentucky, USA Institut für Virologie, Freie Universität Berlin, Germany. URUGUAY ToR 6: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases 11. Did your aboratory collect epizootiological data relevant to international disease control? 12. Did your laboratory disseminate epizootiological data that had been processed and analysed? 13. What method of dissemination of information is most often used by your laboratory? OIE Reference aboratory Reports Activities,
8 (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: 5 Perglione CO, Gildea S, Rimondi A, Miño S, Vissani A, Carossino M, Cullinane A, Barrandeguy M. Epidemiological and virological findings during multiple outbreaks of equine influenza in South America in Influenza Other Respiratory Viruses Jan;10(1): doi: /irv Yamanaka T, Nemoto M, Bannai H, Tsujimura K, Kondo T, Matsumura T, Gildea S, Cullinane A. Evaluation of twenty-two rapid antigen detection tests in the diagnosis of Equine Influenza caused by viruses of H3N8 subtype. Influenza Other Respiratory Viruses Mar;10(2): doi: /irv Gildea S, Sanchez Higgins MJ, Johnson G, Walsh C, Cullinane A. Concurrent vaccination against equine influenza and equine herpes virus - a practical approach. Influenza and Other Respiratory Viruses 2016 Sep;10(5): doi: /irv Yamanaka T, Nemoto M, Bannai H, Tsujimura K, Kondo T, Matsumura T, Gildea S, Cullinane A. Assessment of antigenic difference of equine influenza virus strains by challenge study in horses. Influenza Other Respiratory Viruses Nov;10(6): doi: /irv Paillot R, Rash N, Garrett D, Prowse-Davis, Montesso F, Cullinane A, emaitre, Thibault JC, Wittreck S, Dancer A. How to Meet the ast OIE Expert Surveillance Panel Recommendations on Equine Influenza (EI) Vaccine Composition: A Review of the Process Required for the Recombinant Canarypox-Based EI Vaccine. Pathogens Nov 25;5(4). pii: E64. Review. b) International conferences: 3 ower Airways Meeting, Newmarket, UK February 18th. Oral presentation: Equine Influenza and Herpesvirus Vaccination - Current Evidence for Vaccination of the Performance Horse 10th International Equine Infectious Diseases Conference,Buenos Aires, Argentina April 4th-8th. Chairperson of Equine Influenza Session; Oral presentation: Equine Influenza Vaccination Working Towards an Evidence Based Regime. Participant and Speaker at Federation of European Equine Veterinary Associations Disease Surveillance Network Meeting, Warsaw, Poland September 30th. Oral presentation: Equine Influenza; Update in laboratory techniques, virus surveillance and vaccination. c) National conferences: 2 Autumn/Winter Veterinary ecture Series for Irish Thoroughbred Breeders Association, 8th November, Oral Presentation; Vaccination, Sales and Breeding University College Dublin Squared Meeting, 28th June, Oral Presentation; Science Based Vaccination d) Other: (Provide website address or link to appropriate information) 1 International Collating Centre, Animal Health Trust, Newmarket. ToR 7: To provide scientific and technical training for personnel from OIE Member Countries To recommend the prescribed and alternative tests or vaccines as OIE Standards 14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries? a) Technical visits: 1 b) Seminars: 1 8 OIE Reference aboratory Reports Activities, 2016
9 c) Hands-on training courses: 0 d) Internships (>1 month): 1 Type of technical training provided (a, b, c or d) Country of origin of the expert(s) provided with training No. participants from the corresponding country a Malaysia 1 b Tutor provided BTSF Workshop Brazil Argentina, Brazil, Chile, Colombia, Costa Rica,Ecuador, Paraguay, Uruguay Argentina (4), Brazil (12), Chile (6), Colombia (6), Costa Rica(2), Ecuador (2), Paraguay(4), Uruguay(4) d Japan 1 ToR 8: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned 15. Does your laboratory have a Quality Management System certified according to an International Standard? Quality management system adopted ISO17025 Certificate scan (PDF, JPG, PNG format) The-Irish-Equine-Foundation-td-151T.pdf 16. Is your laboratory accredited by an international accreditation body? Test for which your laboratory is accredited Single radial haemolysis Haemagglutination Inhibtion Real Time RT-PCR Accreditation body INAB INAB INAB 17. Does your laboratory maintain a biorisk management system for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Chapter 1.1.4) OIE Reference aboratory Reports Activities,
10 ToR 9: To organise and participate in scientific meetings on behalf of the OIE 18. Did your laboratory organise scientific meetings on behalf of the OIE? National/ International Title of event Co-organiser Date (mm/yy) ocation No. Participants International Expert Surveillance Panel Meeting Gounalan Pavade 1st March OIE Headquarters Did your laboratory participate in scientific meetings on behalf of the OIE? Title of event Date (mm/yy) ocation Role (speaker, presenting poster, short communications) Title of the work presented Veterinary Seminar Series 1st April 2016 Veterinary Faculty, Montevideo, Uruguay Speaker The Role of an OIE Reference aboratory in the Control of Equine Rhinopneumonitis and Equine Influenza Special Session on International Movement of High Health High Performance Horses 6th April th Equine Infectious Diseases Conference, Buenos Aires, Argentina Speaker Equine Influenza and the HHP Horse BTSF Workshop on Equine Diseases 28th Nov to 1st December 2016 Niteroi, Rio de Janeiro,Brazil Speaker General principles of the OIE s High health, High Performance (HHP) horse concept and the partnership approach developed by the OIE, FEI, IFHA to facilitate international competition horse movement. aboratory Role in Surveillance for Equine Diseases Relevant to Trade ToR 10: To establish and maintain a network with other OIE Reference aboratories designated for the same pathogen or disease and organise regular inter-laboratory 10 OIE Reference aboratory Reports Activities, 2016
11 proficiency testing to ensure comparability of results 20. Did your laboratory exchange information with other OIE Reference aboratories designated for the same pathogen or disease? 21. Was your laboratory involved in maintaining a network with OIE Reference aboratories designated for the same pathogen or disease by organising or participating in proficiency tests? Purpose of the proficiency tests: 1 Role of your Reference aboratory (organiser/ participant) No. participants Participating OIE Ref. abs/ organising OIE Ref. ab. Harmonisation. Maintenance of IS Accreditation for Irish Equine Centre for Single Radial Haemolysis Participant 2 Irish Equine Centre/Animal Health Trust Harmonisation. Maintenance of IS Accreditation for Irish Equine Centre for Haemagglutination Inhibition Participant 2 Irish Equine Centre/Animal Health Trust Harmonisation. Maintenance of IS Accreditation for Irish Equine Centre for real time RT-PCR Participant 2 Irish Equine Centre/Animal Health Trust 1 validation of a diagnostic protocol: specify the test; quality control of vaccines: specify the vaccine type, etc. 22. Did your laboratory collaborate with other OIE Reference aboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest? Title of the project or contract Scope Name(s) of relevant OIE Reference aboratories Validation study on real time RT-PCR diagnostic assay(s) for equine influenza in horses. To validate an equine influenza real time RT-PCR assay(s) for equine influenza viral RNA detection in horses for the intended purpose of certifying freedom from infection in individual animals for trade or movement. Animal Health Trust, Newmarket, U.K. Gluck Equine Research Centre, Kentucky, USA Institut für Virologie, Freie Universität Berlin, Germany. Evaluation of current equine influenza vaccination protocols prior to shipment, guided by the OIE Standards The aim of this study is to provide a sciencebased rationale to identify the ideal time period for vaccination prior to shipment, in support of the harmonisation of the FEI and IFHA recommendations with regard to equine influenza vaccination prior to shipment, guided by the OIE Standards. Animal Health Trust, Newmarket, U.K. OIE Reference aboratory Reports Activities,
12 ToR 11: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference aboratories for the same pathogens and diseases to ensure equivalence of results 23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference aboratories for the same disease? Note: See Interlaboratory test comparisons in: aboratory Proficiency Testing at: see point 1.3 Purpose for inter-laboratory test comparisons 1 No. participating laboratories Region(s) of participating OIE Member Countries Assistance with setting up SRH assay and determining capability 2 Africa Americas Asia and Pacific Europe Middle East Determining capability Haemagglutination Inhibition 2 Africa Americas Asia and Pacific Europe Middle East ToR 12: To place expert consultants at the disposal of the OIE 24. Did your laboratory place expert consultants at the disposal of the OIE? Kind of consultancy ocation Subject (facultative) Revision of Health Certificate for International Movement of HHP Horses remote Vaccination against equine influenza Review of Manual chapter concerning equine influenza Remote Response to comments from Member coutries 25. Additional comments regarding your report: In excess of 1500 SRH assays performed to monitor response to vaccination. Viruses provided to European vaccine company to develop vaccine containing up to date strains in accordance with OIE Recommendations. 12 OIE Reference aboratory Reports Activities, 2016
13 OIE Reference aboratory Reports Activities,
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