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1 oi:1.138/nture1134 CS+ CS- MCH 3 OCT OCT 3 MCH CS- CS+ OCT MCH 3 MCH OCT 3 OCT vs MCH OCT vs MCH ppetitive memory (PI) A 1-1 Unpire onitioning DDC-GAL4/UAS-Trp UAS-Trp/+ -2 MCH OCT OCT MCH sugr OCT MCH MCH OCT OCT vs MCH OCT vs MCH Appetitive memory (PI) Sugr onitioning DDC-GAL4/UAS-Trp UAS-Trp/+ 6 s sugr e HL9-GAL4 f Appetitive memory (PI) Ativtion (2 min memory) *** *** HL9-GAL4/+ HL9-GAL4/UAS-Trp UAS-Trp/+ g DDC-GAL4 TH-GAL8 h Appetitive memory (PI) Ativtion (2 min memory) *** *** *** ** UAS-Trp/+ DDC-GAL4/UAS-Trp DDC-GAL4/TH-GAL8UAS-Trp TH-GAL8UAS-Trp/+ Supplementry Figure 1 Control ehviourl experiments for thermo-tivtion with DDC-GAL4., The protool for unpire thermo-tivtion (see lso Methos)., Unpire thermo-tivtion with DDC-GAL4 i not inue ppetitive oour memory. -, The lk of ppetitive memory in geneti ontrol flies (i.e., n UAS-Trp/+) is not ue to generl lerning efet, s their ppetitive memory with sugr rewr is not impire., The protool for sugr-inue memory (see Methos for etil)., Sugr-inue memory of DDC-GAL4/UAS-TrpA1 is omprle to the ontrols. e, Expression pttern of HL9-GAL4 in the rin (Frontl view, orsl up) is visulize y 1
2 RESEARCH SUPPLEMENTARY INFORMATION UAS-mCD8::GFP (green) with ounterstining of neuropile (mgent). The MB is outline, n the ell oies of PAM neuro re inite with rrowhes. Sle r 2 μm. f, Thermo-tivtion with HL9-GAL4 inues signifint ppetitive memory. n= g, Expression pttern of DDC-GAL4 TH-GAL8. h, TH-GAL8 i not lok ppetitive memory y thermo-tivtion with DDC-GAL4. Men n s.e.m. re shown (,, f n h). Appetitive memory (PI) Feeing (hr) Strvtion Sugr onitioning (3h memory) ** * *** Wiltype without feeing DDC-GAL4/UAS-Shi UAS-Shi/+ Supplementry Figure 2 Stition les to the suppression of ppetitive memory. Brief feeing efore the test suppresses the ehviourl expression of sugr-inue memory. Bloke in DDC-GAL4/UAS-shi ts1 flies uring feeing oes not reverse feeing-meite suppression of onitione oour pproh. n=
3 RESEARCH DDC-GAL4/UAS-shi UAS-shi/+ Appetitiv ve memory (PI) (min) Sugr onitioning ** *** Appetitiv ve memory (PI) (min) Sugr onitioning App petitive memory (PI) (min) Sugr onitioning referene Inex P (min) Sugr preferene * Supplementry Figure 3 Aquisition of sugr-rewre memory requires the output of neuro in DDC-GAL4., A trient lok uring the trining (lue shing) inue in DDC-GAL4/UASshi ts1 y 3-min temperture elevtion signifintly impirs sugr-inue olftory memory. No signifint efet is foun uner the sme lok pplie fter trining () or t permissive temperture (). n=15-16 (-)., There is no oistent efet in DDC-GAL4/UAS-shi ts1 sugr preferene t restritive temperture. n=
4 RESEARCH SUPPLEMENTARY INFORMATION R58E2-GAL4 R58E2-GAL4 ex Preferene in Sugr preferene * * (min) R58E2-GAL4/+ R58E2-GAL4/UAS-shi UAS-shi/+ Supplementry Figure 4 Expression pttern of R58E2-GAL4 n sugr preferene uner loke of these neuro., The whole-mount rin expressing UAS-mCD8::GFP (green) uner the ontrol of R58E2-GAL4 with ounterstining with the nti-th (mgent). R58E2-GAL4 prominently lels the PAM luster neuro n gli ells in the opti loes with little itionl expression., Mgnifition of the region inluing the MB loes (outline). The PAM luster neuro supply ee rours in the meil loes of the MB., Bloke of the output of the PAM neuro in R58E2-GAL4/UAS-shi ts1 oes not impir the reflexive hoie of sugr. n=
5 RESEARCH Brin f g PAM luster e h DDC-GAL4 R58E2-GAL4 Supplementry Figure 5 Serotonin-immunoretivity is unetetle in the PAM luster neuro in DDC-GAL4 n R58E2-GAL4. Doule lelling of serotonin (mgent) n DDC-GAL4 (, -e) or R58E2-GAL4 (, f-h) visulize with UAS-mCD8::GFP (green). -f, There is no etetle ololiztion in the ell oies of the PAM luster neuro (, e n g, h re split hnnels of n f,, respetively). 5
![[mgent]).](/docs-images/81/82767661/images/6-3.jpg "The PAM luster neuro n glil ells in the opti loes re strongly lelle")
6 RESEARCH SUPPLEMENTARY INFORMATION R58E2-LexA TH R58E2-GAL4 TH R58E2-LexA R58E2-GAL4 e R58E2-LexA R58E2-GAL4 R58E2-GAL4 R58E2-GAL8 Supplementry Figure 6 Coisteny of the regultory element R58E2 when ierte t ifferent genomi lotio. -, R58E2-LexA (ierte in ttp4) () n R58E2-GAL4 (ierte in ttp2) () hve similr expression ptter (Frontl view, orsl up; ounterstining with TH [mgent]). The PAM luster neuro n glil ells in the opti loes re strongly lelle (green). Sle rs 2 μm.,, Doule lelling of R58E2-LexA n R58E2-GAL4 in single rin visulize using pjfrc21-1xuas-ivs-mcd8::rfp (ttp18; re), pjfrc15-13xlexaop2-mcd8::gfp 13XL CD8 (su(hw)ttp8;(h ) green). The expression pttern in the PAM luster is lrgely overlpping (), lthough R58E2- GAL4 lels few more ells (rrow in ). e, R58E2-GAL8 suppresses expression of UAS-mCD8::GFP in most, if not ll, PAM neuro in R58E2-GAL4. 6
7 RESEARCH R58E2-LexA LexAop2- UAS- NP5272-GAL4 mcd8::gfp mcd8::rfp e f Supplementry Figure 7 Heterogeneity of the PAM luster neuro. -f, Projetion of severl onfol slies (~3 µm) for mgnifie ell oy regio of MB-M3. The ell oies of MB-M3 neuro (rrowhes) visulize with mcd8::rfp (mgent) uner the ontrol of NP5272 o not overlp with R58E2-LexA-lelle neuro visulize with mcd8::gfp (green). 7
8 RESEARCH SUPPLEMENTARY INFORMATION Centrl rin PAM mcd8::gfp mcd8::rfp R58E2-LexA TH-GAL4 e f g h NP5272 R58E2-LexA TDC2-GAL4 Supplementry Figure 8 Neuro of the PAM luster n the OA-ASM ASM luster in TH-GAL4 n TDC2-GAL4. -, The PAM neuro lelle in R58E2-LexA (green) n TH-GAL4 (mgent) re visulize y mcd8::gfp n mcd8::rfp, respetively. Severl, ut not ll, neuro re lelle y oth rivers (see lso Fig. 4). Soli n hollow rrowhes inite non-overlpping n overlpping neuro, respetively. e-h, The PAM neuro n the OA-ASM neuro lelle with R58E2-LexA (green) n TDC2-GAL4 (mgent), respetively, o not overlp. Arrowhes inite the ell oies of TDC2-GAL4 lelle neuro. Supplementry Figure 9 Experimentl setup of omine in vivo lium imging n gusttory stimultion. A fly is prepre for in vivo imging on ustom-me essile surgery ell. The ell is ple uner the ojetive of onfol mirosope 33. A roplet of wter, surose or ffeine solution is eposite on plsti plte riven y miromnipultor. To eliver the stimulus, the plte ws lifte up to rehle istne for the fly. 8
9 RESEARCH Supplementry Movie 1 The expression pttern of R58E2-GAL4 in the rin R58E2-GAL4 visulize with UAS-mCD8::GFP (green) with neuropile ounterstining using the nti-synpsin ntioy (mgent). Supplementry Movie 2 The expression pttern of DDC-GAL4 in the entrl rin mcd8::gfp (green) is expresse uner the ontrol of DDC-GAL4 with ounterstining with the nti-th ntioy (mgent) in the entrl rin. Supplementry Movie 3 The expression pttern of DDC-GAL4 with R58E2- GAL8 in the entrl rin R58E2-GAL8 silenes trgene expression of most PAM luster neuro in DDC- GAL4 visulize with UAS-mCD8::GFP (green). The rin is ounterstine with the nti-th ntioy (mgent). Supplementry Movie 4 Differentil lelling of NP5272-GAL4 n R58E2- LexA in the ell oy region of the PAM luster mcd8::gfp (green) n mcd8::rfp (mgent) re expresse uner the ontrol of R58E2-LexA n NP5272-GAL4, respetively. Supplementry Movie 5 Differentil lelling of TH-GAL4 n R58E2-LexA in the MB mcd8::gfp (green) n mcd8::rfp (mgent) re expresse uner the ontrol of R58E2-LexA n TH-GAL4, respetively. 9
LHb VTA. VTA-projecting RMTg-projecting overlay. Supplemental Figure 2. Retrograde labeling of LHb neurons. a. VTA-projecting LHb
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mch log height Counts GFP log height mch log height Counts GFP log height mch log height Counts high flux % Hist: 8.34 EBSS shctrl Counts
DOI:.8/n2886 mchrry-gfp-lc3 Autophgy Rportr: Autophgosom Autolysosom mchrry GFP LC3 mchrry GFP LC3 X ph >6. ph
Non-redundant odor coding by sister mitral cells revealed by light addressable glomeruli in the mouse
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Control vector. HA-Elfn2. HA-Elfn1
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Imaging analysis of clock neurons reveals light buffers the wake-promoting effect of dopamine
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SOME MECHANISTIC CONCEPTS IN ELECTROPHILIC ADDITION REACTIONS TO C=C BONDS
SM MANISTI NPTS IN LTPILI AITIN ATINS T = BNS The = ond is considered to e wek se/nucleophile. The high concentrtion of electron density mkes the pi ond Lewis se, ut in order to donte electrons the pi
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Coatomer LPAAT-γ Merge
DI: 1.138/n2273 Cotomr LPAAT-γ Mrg Tuuls (%) 1 5 < 5 nm 5~1nm 1~2nm 2~5nm >5nm 5~1nm < 5 nm 1~2nm 2~5nm >5nm 2 min 5 min 1 min 3 min < 5 nm 5~1nm 1~2nm 2~5nm >5nm < 5 nm 5~1nm 1~2nm 2~5nm >5nm Figur S1
Supporting Information:
Supporting Informtion: Unlne reution of nutrient los hs rete n offshore grient from phosphorus to nitrogen limittion in the orth Se Authors: Amn Burson, Myke Stomp, Lriss Akil, orin.d. Brussr, n Jef Huismn
Human body epigenome maps reveal noncanonical DNA methylation variation
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( 6.48) SECTION S-S LEGS DIMENSION (2X) WIDTH: THICKNESS:
1 3 4 PRT NUMER ONTT RE PLTING 1LF.75um GOL 1FLF GOL FLS 6.65.63 M3.5 1.. 3..38 8.1 ( 9.) OF IM..9 ( 6.48).1 S 11.45 1.45 7.9 R.4.1 7.5 14.5 6.5.9 13.5.9 S Z 1.9 Y 3.3.5 SETION SS SEE ETIL.9 3.18 1.51.1
Visualization of Stent Lumen in MR Imaging: Relationship with Stent Design and RF Direction
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Supplementary Figure 1
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Supplementary Information
Supplementary Information Supplementary s Supplementary 1 All three types of foods suppress subsequent feeding in both sexes when the same food is used in the pre-feeding test feeding. (a) Adjusted pre-feeding
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Supplementary Figure S1
Supplementry Figure S1 d MAP2 GFAP e MAP2 GFAP GFAP c f Clindin GFAP Supplementry Figure S1. Neuronl deth nd ltered strocytes in the rin of n ffected child. Neuron specific MAP2 ntiody stining in the hippocmpus
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1 3 4 4.00 PIN#11 1.30 KE W G 5.80 1.45 + 0.07 PIN#40 7.60 3.60 0.88#0.10 ONTT GP.0 PIN#1 1.000 133.75 0.10 SLOT LENGH PIN#10 E 147.08 REF.(160 M. LTH OPEN) 18.8 MIN. TE OE 7 SETING HEIGHT.30 0.10 0.10
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100 μm. Axon growth cones. Tubulin (red) + scr (green)
Supplementary Figures mirorna-9 regulates axon extension an ranhing y targeting Map1 in mouse ortial neurons Dajas-Bailaor, F., Bonev, B., Garez, P., Stanley, P., Guillemot, F., Papalopulu, N. a 2 μm 1
Supplemental Figures and Legends
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SUPPLEMENTARY INFORMATION
doi: 10.1038/nture07679 Emryonic Stem (ES) cell Hemngiolst Flk1 + Blst Colony 3 to 3.5 Dys 3-4 Dys ES differentition Sort of Flk1 + cells Supplementry Figure 1. Chrcteristion of lst colony development.
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SUPPLEMENTARY INFORMATION
oi:1.138/nture11943 E12.5-CON E12.5-KO b E14.5-CON E14.5-KO Supplementry Figure S1 Loss of Bmpr1 in the Myf5 + linege oes not result in obvious morphologicl chnges prior to E16.5., b, Hemtoxylin & Eosin
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SUPPLEMENTARY INFORMATION
doi: 1.138/nture862 humn hr. 21q MRPL39 murine Chr.16 Mrpl39 Dyrk1A Runx1 murine Chr. 17 ZNF295 Ets2 Znf295 murine Chr. 1 COL18A1 -/- lot: nti-dscr1 IgG hevy hin DSCR1 DSCR1 expression reltive to hevy
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Chapter 5: The peripheral nervous system Learning activity suggested answers
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b PolyA RNA-seq c RNA-seq read distribution FPKM
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SUPPLEMENTARY INFORMATION
doi:0.08/nture0987 SUPPLEMENTARY FIGURE Structure of rbbit Xist gene. Exons re shown in boxes with romn numbers, introns in thin lines. Arrows indicte the locliztion of primers used for mplifiction. WWW.NATURE.COM/NATURE
Striatum CA
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