Your single-source laboratory solution. Antibody Library

Transcription

1 Your single-source laboratory solution. Antibody Library

2 A-ACT Alpha-1 antichymotrypsin; histiocytes; pancreatic pseudopapillary tumor A-AT Alpha-1-antitrypsin; inherited AAT deficiency ACTH Adrenocorticotropic hormone; anterior pituitary Actin - Muscle Specific Actin - Smooth Muscle (SMA) Skeletal, smooth, and cardiac muscle; myofibroblastic differentiation Myofibroblasts; myoepithelial cells Adenovirus Adenovirus Adipophilin Identification of sebaceous neoplasms AE1 Cytokeratin AE1; epithelial marker AE1/AE3 Screening cytokeratin cocktail; epithelial marker AE3 Cytokeratin AE3; high molecular weight cytokeratin; epithelial marker AFP Alpha fetoprotein; hepatocellular carcinoma; hepatoid carcinoma; yolk sac tumor AKT Phosphate AKT (protein kinase B) ALK (D5F3) FDA-approved CDx; identifies NSCLC patients eligible for crizotinib ALK-1 Anaplastic lymphoma kinase-1; anaplastic large-cell lymphoma; inflammatory myofibroblastic tumor; adenocarcinoma of lung subset Alpha Beta Flow T-cell receptor Amyloid A Amyloid A protein Androgen Receptor Prostate carcinoma; associated with prognosis and treatment outcome Annexin A1 Hairy cell leukemia; myeloid cells Arginase-1 Benign and hepatocellular carcinoma (HCC) B72.3 Simple epithelium; adenocarcinoma; excludes mesothelioma BCA-225 Breast carcinoma-associated glycoprotein; breast carcinoma and other adenocarcinoma BCL-1 (Cyclin D1) Mantle cell lymphoma; multiple myeloma subset BCL-1/CD20 Double stain; useful for the diagnosis of mantle cell lymphoma 1

3 BCL-2 /Flow Follicular lymphoma; other B- and T-cell lymphoma; soft tissue tumor BCL-2/BCL-6 Double stain; useful for the diagnosis of follicular center cell lymphoma, diffuse large-cell type BCL-6 Follicular center B-cells; follicular lymphoma; DLBCL subset Ber-EP4 /Flow Epithelial antigen; carcinoma; differentiate carcinoma from mesothelioma Beta Amyloid Alzheimer s disease; senile plaques; neurofibrillary tangles; cerebrovascular deposits Beta-Catenin Soft tissue lesion; fibromatosis; synovial sarcoma BG8 (Lewis Y) Adenocarcinoma; exclude mesothelioma BOB-1 B-cells CA 19.9 Pancreas, GI, ovary, lung, and transitional cell carcinoma CA 125 (OC125) Female genital tract; breast carcinoma; other adenocarcinoma including lung and pancreatobiliary CAIX Carbonic anhydrase IX; renal cell carcinoma (RCC); distinguish clear cell renal cell carcinoma from other RCC subset Calcitonin Thyroid C-cells; medullary carcinoma Caldesmon Smooth muscle and myoepithelial cells Calponin Myoepithelial, myofibroblastic, and smooth muscle cells Calretinin Mesothelial cells; sex cord stromal tumor; adrenal cortical tumor CAM 5.2 Low molecular weight cytokeratin 8; epithelial neoplasm CAM 5.2/AE1 Screening cytokeratin cocktail; epithelial tumor CD1a /Flow Cortical thymocytes; Langerhans cell histiocytosis; T-ALL subset CD2 /Flow Pan, thymic, and peripheral T-cells; NK cells; neoplastic mast cells CD3 /Flow Pan T-cells CD3/CD20 Double stain; useful to distinguish B-cells (CD20+) and T-cells (CD3+) CD4 /Flow Helper/inducer T-cells; thymocyte and monocyte subsets CD5 /Flow Pan T-cells; B-cell lymphoma subset (B-CLL/SLL and MCL); thymic carcinoma 2

4 CD7 /Flow Pan T-cells; NK cells, thymocytes; rare AML CD8 /Flow Suppressor/cytotoxic T-cells; thymocyte and T-/NK cell subsets; T-LGL leukemia CD10 /Flow CALLA; B- and T-cell lymphoma subsets; endometrial stromal cells; renal cell carcinoma subset CD11a Flow Leukocytes; utilized in the assessment for leukocyte adhesion deficiency (LAD) CD11b Flow Granulocytes; monocytes CD11c Flow Monocytes/macrophages; hairy cell leukemia; AML subset CD13 Flow Monocytes/macrophages; myeloid cells CD14 /Flow Mature monocytes; some granulocytes; macrophages CD15 /Flow Epithelial, myeloid, and Reed-Sternberg cells; Hodgkin lymphoma CD16 Flow NK cells; granulocytes; neutrophils; monocytes CD18 Flow Leukocytes; utilized in the assessment for leukocyte adhesion deficiency (LAD) CD19 Flow Pan B-cell marker CD20 /Flow Pan B-cells CD21 Follicular dendritic cells; B-cell subset CD22 /Flow Pan B-cells CD23 /Flow Follicular dendritic cells; B-cell subset; B-CLL CD24 Flow Variety of leukocytes; GPI-linked; utilized in the assessment for PNH CD25 /Flow Activated T- and B-cells; HCL; ATLL CD26 Flow Evaluation of mycosis fungoides and Sezary syndrome CD30 /Flow Reed-Sternberg cells; anaplastic large-cell lymphoma; DLBCL subset; Hodgkin lymphoma; embryonal carcinoma CD31 Endothelium; vascular neoplasm; plasma cells CD33 /Flow Monocytes; myeloid cells; AML; ALL subset CD34 /Flow Blasts; stem cells; endothelium; GIST; soft tissue tumor subset CD35 Follicular dendritic cells CD38 Flow Plasma cells; activated T-, B-, and myeloid cells; epithelial tumor subset CD41a Flow Platelets; megakaryocytes 3

5 CD42a Flow Megakaryocytes; platelets CD43 /Flow T-cells; B-cell lymphoma subset (B-CLL/SLL, MCL, Burkitt); plasma and myeloid cells; histiocytes CD45 (LCA) /Flow Leukocyte common antigen CD45RA B-cells CD45RO Activated/memory T-cells; B-cell subset CD52 Flow Thymocytes; lymphocytes; monocytes; macrophages CD55 Flow Leukocytes; erythrocytes CD56 /Flow Neural and neuroendocrine tumor; soft tissue tumor subset; NK cells; plasma and T-cell subsets; LGL leukemia CD57 /Flow Neural and neuroendocrine tumor; thymoma; NK cells; T-LGL leukemia; T-cell subset CD59 Flow Leukocytes; erythrocytes; PNH CD61 /Flow Megakaryocytes; platelets CD62 Flow Activated platelets CD64 Flow Monocytes; macrophages CD68 Histiocytes; macrophages CD71 /Flow Erythroid precursors and large-cell lymphoma; transferrin receptor; activated T- and B-cells; macrophages CD74 B-cells CD79a /Flow Pan B-cells CD79b Flow A component of the CLL MRD panel CD81 Flow Expressed by many tissue types, including hematopoietic cells; a component of the CLL MRD panel CD99 PNET/Ewing sarcoma; soft tissue tumor subset; sex cord stromal tumor CD103 Flow Hairy cell leukemia; enteropathy type T-cell lymphoma CD117 (c-kit) /Flow GIST; subset of carcinoma including adenoid cystic carcinoma; germ cell neoplasm; hematolymphoid neoplasm CD123 Dendritic cell precursors; type 2 (DC2) cells; blastic plasmacytoid dendritic cell neoplasm (BPDCN) CD138 /Flow Plasma cells; subset of epithelial cells CD158a Flow Subsets of NK cells and T-cells; utilized in the assessment of NK cell proliferations 4

6 CD158b Flow Subsets of NK cells and T-cells; utilized in the assessment of NK cell proliferations CD158e Flow Subsets of NK cells and T-cells; utilized in the assessment of NK cell proliferations CD163 Monocytes; macrophages; dendritic interstitial cells CDK4 Atypical lipomatous tumor and dedifferentiated liposarcoma CDX-2 Enteric differentiation; GI tumor; colorectal carcinoma; carcinoid tumor subset CDX-2/CK-7 Double stain; useful to distinguish stomach and colon carcinoma from breast, lung, and ovarian carcinoma CEA (M) Carcinoembryonic antigen (monoclonal); adenocarcinoma CEA (P) Carcinoembryonic antigen (polyclonal); epithelial marker; adenocarcinoma subset; negative in mesothelioma Chromogranin A Neuroendocrine neoplasm; paraganglioma Chymotrypsin Exocrine pancreas; acinar cell tumor CK-5 Tumor with squamous differentiation; squamous cell vs. adenocarcinoma of lung classification; myoepithelial cells; benign vs. malignant breast intraductal proliferation CK-5/6 Tumor with squamous differentiation; squamous cell vs. adenocarcinoma of lung classification; myoepithelial cells; benign vs. malignant breast intraductal proliferation CK-7 Various subtypes of carcinoma; frequently used in conjunction with CK-20 for tumor profiling CK-7/TTF-1 Double stain; useful for the diagnosis of lung adenocarcinoma CK-14 High molecular weight cytokeratin; tumor with squamous differentiation; myoepithelial cells; breast carcinoma with basal-like phenotype CK-17 High molecular weight cytokeratin; tumor with squamous differentiation; pancreatobiliary adenocarcinoma; breast carcinoma with basal-like phenotype CK-18 Low molecular weight cytokeratin CK-19 Epithelial tumor; papillary carcinoma of the thyroid vs. benign thyroid lesion CK-20 Subset of carcinoma; reacts primarily with gastric and intestinal epithelium, urothelium, and Merkel cells 5

7 CK-HMW (34betaE12) CK-LMW (35betaH11) CK-903; broad-spectrum high molecular weight cytokeratin; squamous cell and subsets of adenocarcinoma and transitional cell carcinoma; prostate basal cells; prostate carcinoma vs. adenosis/pin CK8/CK18; epithelial neoplasm with simple epithelium cmet Non-small cell lung carcinoma CMV Cytomegalovirus cmyc Burkitt Lymphoma and some large B-cell lymphomas COLL-IV Collagen type IV; basement membrane material; soft tissue tumor subset COX-2 Cyclooxygenase-2; prognostic and predictive marker in a variety of tumors Cytokeratin FLOW CD45 non-hematopoietic cells D2-40 Evaluation of lymphatic endothelial differentiation for lymphatic invasion; subset of angiosarcoma; vascular tumor of lymphatic origin; mesothelioma DBA44 (HCL) Hairy cell leukemia Desmin Striated and smooth muscle; leiomyosarcoma; rhabdomyosarcoma; desmoplastic small round cell tumor DOG1 GIST, in particular CD117-negative lesion EBER - ISH ISH Epstein-Barr early RNA; Epstein-Barr virus identification EBV (LMP1) Epstein-Barr virus; latent membrane protein E-Cadherin Ductal vs. lobular lesion of breast; positive in ductal lesion and negative in lobular neoplasia EGFR Epidermal growth factor receptor; colon and lung carcinoma; basal-phenotype breast carcinoma EMA Epithelial membrane antigen; epithelial marker; subset of sarcoma, meningioma, and hematolymphoid neoplasm ER Estrogen receptor; predictive of endocrine therapy response in breast carcinoma; lower female genital tract carcinoma; endometrial stromal neoplasm; soft tissue lesion subset Factor VIII (vwf) Von Willebrand factor; endothelial marker; vascular neoplasm; blast cells in acute megakaryocytic leukemia Factor XIIIa Dermal dendrocytes and dendritic interstitial cells; used with CD34 to differentiate between dermatofibroma and dermatofibrosarcoma protuberans; fibrohistiocytic neoplasm 6

8 Fascin Hodgkin and Reed-Sternberg cells FLAER Flow Binds to GPI anchor on leukocytes; utilized in the assessment for PNH FLI-1 Endothelial vascular cells/tumor; Ewing sarcoma/pnet; lymphoblastic lymphoma/leukemia FMC7 Flow B-cell neoplasm; mantle cell lymphoma FOXP1 Subclassification of large B-cell lymphomas FSH Follicular-stimulating hormone; anterior pituitary Galectin-3 Benign vs. malignant thyroid follicular neoplasm, in particular papillary carcinoma Gamma Delta Flow Gamma delta T-cell receptor; T-cell subset Gastrin Gastric carcinoid; pancreatic endocrine neoplasm subset GATA-3 Breast and urothelial carcinomas GCDFP-15 Gross cystic disease fluid protein-15; breast carcinoma; salivary and sweat gland neoplasm GFAP Glial fibrillary acidic protein; glioma; lesion with glial differentiation; salivary gland tumor; neural neoplasm subset Glucagon Pancreatic endocrine neoplasm Glycophorin A /Flow Erythroid cells Glypican-3 Hepatocellular carcinoma; germ cell tumor; yolk sac tumor Granzyme B Cytotoxic T-cell subset; NK cells Growth Hormone (GH) Growth hormone; anterior pituitary GST Distinguish Hodgkin disease and histiocytic malignancy from T-cell lymphoma HAM 56 Histiocytes HBME-1 Mesothelial cells; subset of adenocarcinoma, in particular lower female genital tract origin; papillary carcinoma of the thyroid HBP (H. pylori) Helicobacter pylori HCG Human chorionic gonadotropin (beta); germ cell tumor; trophoblastic disease Hemoglobin A Red cell precursors HEP B CORE (HBcAg) Hepatitis B core antigen; hepatitis B 7

9 HEP B SURF (HBsAg) Hepatitis B surface antigen; hepatitis B HepPar 1 Hepatocellular carcinoma; hepatoid tumor; germ cell tumor HER2 HER2 protein overexpression; FDA-approved test for detection of HER2/neu; prognostic and predictive indicator in breast carcinoma HER2 - ISH ISH HER2 gene amplification; FDA-approved test for detection of HER2 amplification; prognostic and predictive indicator in breast carcinoma Herpes I/II Double stain; Herpes simplex virus type I/II HGAL Subclassification of large B-cell lymphomas; diagnosis of follicular lymphoma HHV-8 Human herpesvirus 8; Kaposi s sarcoma; primary effusion lymphoma HLA-DR /Flow Human leukocyte antigen; MHC class II antigen; B-cells; monocytes; myeloid cells; AML; ALL HMB-45 Melanoma-associated marker; melanocytic lesion; pecoma HPL Human placental lactogen; intermediate trophoblasts; placental site trophoblastic lesion HPV (6/11) - ISH ISH Low-risk human papilloma virus subtypes HPV (16/18) - ISH ISH High-risk human papilloma virus subtypes HPV (31/33) - ISH ISH High-risk human papilloma virus subtypes IgA /Flow Immunoglobulin A heavy chain IgD /Flow Immunoglobulin D heavy chain IgG /Flow Immunoglobulin G heavy chain IgG4 IgG4-related sclerosing disease IgM /Flow Immunoglobulin M heavy chain Inhibin Sex cord stromal and adrenal cortical tumor; steroid lesion of the ovary Insulin Pancreatic endocrine neoplasm JC Virus - ISH ISH Progressive multifocal leukoencephalopathy virus Kappa /Flow Immunoglobulin light chain; B-cells and plasma cells Kappa - ISH ISH Immunoglobulin light chain; B-cells and plasma cells RNA KER, PAN Broad-spectrum cytokeratin cocktail 8

10 Ki-67 Cell proliferation marker Lambda /Flow Immunoglobulin light chain; B-cells and plasma cells Lambda - ISH ISH Immunoglobulin light chain; B-cells and plasma cells RNA LH Luteinizing hormone; anterior pituitary Lipase Exocrine pancreas; acinar cell tumor Lysozyme (MURA) Muramidase; AML; granulocytic sarcoma; histiocytic differentiation Mammaglobin Breast carcinoma; salivary gland lesion and sweat gland neoplasm MART-1 Melanoma-associated marker MBP Myelin basic protein; mucin; pancreatic carcinoma vs. chronic pancreatitis; colorectal carcinoma; endometrial carcinoma; Barrett s esophagus M-Cell Tryptase Mast cells MDM2 Atypical lipomatous tumor and dedifferentiated liposarcoma MEL-5 Melanoma-associated marker Melan-A Melanoma-associated marker; melanocytic lesion and adrenal cortical neoplasm; pecoma Melan-A/Ki-67 Double stain; useful to distinguish malignant melanoma from benign melanocytic proliferation Mesothelin Mesothelioma MiTF Malignant melanoma; melanocytic lesion MLH-1 DNA mismatch repair protein MOC-31 Epithelial differentiation; adenocarcinoma vs. mesothelioma MPO /Flow Myeloperoxidase; AML; granulocytic sarcoma MSH-2 DNA mismatch repair protein MSH-6 DNA mismatch repair protein MUC1 Pancreatobiliary and extra-pancreatobiliary non-mucinous adenocarcinoma MUC2 Colorectal adenocarcinoma and mucinous adenocarcinoma of various sites MUC4 Epithelial tumor (breast, lung, cervix); pancreatic carcinoma 9

11 MUC5AC MUC5AC glycoprotein; distinguish primary mucinous ovarian tumor from colonic carcinoma metastatic to the ovary MUM-1 Plasma cells; post-germinal B-cells; activated T-cells; Reed-Sternberg cells; DLBCL subset MYO DI Rhabdomyosarcoma Myogenin Rhabdomyosarcoma Myoglobin Rhabdomyosarcoma Myosin Heavy Chain (SMM-HC) Smooth-muscle myosin heavy chain; breast myoepithelial cells Napsin A Lung adenocarcinoma NB84a Neuroblastoma tumor cells (for bone marrow aspirate and stem cell products, fresh specimen only) NEU-N Neuronal differentiation NF Neurofilament; Merkel cells and neural tumor NKI-C3 Melanoma-associated marker; melanocytic lesion and neurothekeoma NPM Nucleophosmin; nucleophosmin mutations in AML NSE Neuron-specific enolase; present in neurons and neuroendocrine cells (central and peripheral) OCT-2 B-cell lymphocyte transcription factor; B-cells OCT-3/4 icular germ cell tumor, in particular seminoma and embryonal carcinoma OSCAR Broad-spectrum cytokeratin; epithelial lesion p16 HPV-associated dysplasia/carcinoma (CINtec p16ink4a) p21 Tumor suppressor gene p27/kip1 Cyclin-dependent kinase inhibitor p40 Squamous cell marker p53 Mutated tumor suppressor gene p57 Molar vs. non-molar gestation; distinguish complete hydatidiform mole (CHM) from partial hydatidiform mole (PHM) and hydropic villi p63 Prostatic basal cells; breast myoepithelial cells; squamous cell carcinoma p120 Ductal vs. lobular breast lesion; lobular breast carcinoma 10

12 P504S Prostatic carcinoma and high grade PIN; subset of renal cell carcinoma Pan Melanoma Cocktail Pan Melanoma Double Stain Cocktail (MART-1, tyrosinase, HMB45); for melanoma and melanocytic lesion; pecoma Double Stain (MART-1/Tyrosinase, S100); for melanoma and melanocytic lesion; pecoma PANC.POLY Pancreatic islet-cell tumor subset Parvovirus B19 Parvovirus PAX-2 Renal cell carcinoma PAX-5 B-cell transcription factor; B-cells; Reed-Sternberg cells; subset of carcinoma including small-cell carcinoma PAX-5/BCL2 Double stain; Follicular lymphoma and subsets of diffuse large cell B-cell lymphoma PAX-5/CD43 Double stain; useful for the identification of CD43-coexpressing B-cell lymphoma PAX-5/CD5 Double stain; useful for the identification of CD5-coexpressing B-cell lymphoma PAX-8 Renal cell carcinoma, GYN and follicular derived thyroid carcinoma PC (P. carinii) Pneumocystis carinii PD1 (heme) Angio immunoblastic T-cell lymphoma (AITL); germinal center T-cells PD-L1 (22C3) FDA-approved CDx in NSCLC patients for pembrolizumab. Global service only. PD-L1 (28-8) FDA-approved complementary Dx in non-squamous NSCLC and melanoma for nivolumab. Global service only. PD-L1 (SP142) FDA-approved complementary Dx in urothelium and NSCLC for atezolizumab. Global service only. Perforin Lymphomas of cytotoxic or NK derivation PGP 9.5 Protein gene product 9.5; neuroendocrine tumor PLAP Placental alkaline phosphatase; germ cell tumor; trophoblastic disease and subset of adenocarcinoma PMS-2 DNA mismatch repair protein PR Progesterone receptor; predictive of endocrine therapy response in breast carcinoma; lower female genital tract carcinoma; endometrial stromal neoplasm; soft tissue lesion subset 11

13 Prolactin Anterior pituitary hormone PSA Prostate-specific antigen; primary and metastatic prostatic neoplasm PSAP Prostatic acid phosphatase; primary and metastatic neoplasm; carcinoid tumor subset PSMA Prostate adenocarcinoma PTEN Cowden syndrome; carcinoma of the bladder, breast, endometrium, head and neck, kidney, lung, prostate, stomach, and thyroid; glioblastoma; melanoma PTH Parathyroid hormone; parathyroid neoplasm PTS Prostate triple stain (P504S, p63, CK903); useful for the diagnosis of PIN/adenosis vs. prostate carcinoma RB Retinoblastoma gene protein; retinoblastoma RCC (PNRA) Renal cell carcinoma S100 Neural tumor; melanoma; chondrocytes; epithelial neoplasm subset SALL4 Seminoma, embryonal and yolk sac germ-cell tumors Serotonin Carcinoid; pancreatic endocrine neoplasm Somatostatin Somatostatin; islet cell tumor; carcinoid; pancreatic endocrine neoplasm SOX10 Melanoma and neural neoplasms SOX11 Mantle cell lymphomas; gliomas Surfactant (SP-A) Surfactant protein A; lung adenocarcinoma Synaptophysin Neural and neuroendocrine-derived tumor Synuclein Neurodegenerative diseases (synucleinopathies); Parkinson s disease TAU Neurofibrillary tangles; Lewy bodies TCL-1 Ewing sarcoma/pnet; blastic plasmacytoid dendritic cell neoplasm (BPDCN) TCR BETA /Flow Alpha-beta T-cells TdT /Flow Terminal deoxynucleotidyl transferase; cortical thymocytes; B-ALL; T-ALL Thrombomodulin Mesothelial cells; mesothelioma; lymphatic and vascular tumor Thyroglobulin Follicular and papillary carcinoma of the thyroid 12

14 TIA-1 T-cell intracytoplasmic antigen; cytotoxic T-cell subset TLE1 Synovial sarcoma TOXO Toxoplasmosis TRAP Tartrate-resistant acid phosphatase; hairy cell leukemia TriView Breast Breast triple stain (CK5/p63/LMW); useful to differentiate DCIS from microinvasive breast carcinoma; differentiate radial scar from infiltrating carcinoma TriView Prostate Prostate triple stain (P504S, p63, CK903); useful for the diagnosis of PIN/adenosis vs. prostate carcinoma Trypsin Exocrine pancreas; acinar cell tumor TS Thymidylate synthase; prognostic and predictive indicator for 5-FU-based chemotherapy in colorectal carcinoma TSH Thyroid stimulating hormone; anterior pituitary TTF-1 Thyroid transcription factor-1; lung and thyroid carcinoma; subset of small cell carcinoma Tyrosinase Malignant melanoma; melanocytic lesion Ubiquitin Neurofibrillary tangles; Lewy bodies Uroplakin Transmembrane protein expressed by urothelium VEGF Vascular endothelial growth factor; stimulates endothelial cell proliferation; induces microvessel permeability; prognostic indicator Villin Differentiate gastrointestinal adenocarcinoma, neuroendocrine carcinoma, and ovarian adenocarcinoma from other adenocarcinoma; label Merkel cells of the skin Vimentin (VIM) Mesenchymal neoplasms; malignant melanoma and subsets of carcinoma and lymphoma VIP Vasoactive intestinal polypeptide; pancreatic endocrine neoplasm VZV Varicella-Zoster virus WT1 (M) N-Terminus WT1 (P) C-Terminus Wilms tumor protein; mesothelial cells; malignant mesothelioma; ovarian adenocarcinoma; sex cord stromal tumor of the ovary; endometrioid stromal neoplasm Wilms tumor protein carboxyl terminal; desmoplastic small round cell tumor ZAP-70 /Flow T-cells; CLL/SLL prognosis 13

15 Marker Specimen Requirements Immunohistochemistry () Fixed Paraffin Block with corresponding H&E, if available Tissue should be well-fixed in formalin; if alternative fixative is used, it should be noted on the requisition 0.2 cm x 0.2 cm x 0.2 cm tissue Store specimen at room temperature Use cold pack for transport; be sure cold pack is not in direct contact with specimen during transport Unstained Slides Send all slides within 6 weeks of cutting Minimum of 2 slides per antibody Precut slides from paraffin blocks in 5 micron sections Mounted on Poly-L-Lysine coated or plus (+) slides Air dry; do not oven dry Store specimen at room temperature Use cold pack for transport; be sure cold pack is not in direct contact with specimen during transport Body Fluids Process fluids into formalin-fixed, paraffin-embedded cell blocks or cytospin preparation In Situ Hybridization (ISH) Fixed Paraffin Block with corresponding H&E, if available Tissue should be well-fixed in formalin. Please Note: Only formalin-fixed tissue will be accepted for ISH testing 0.2 cm x 0.2 cm x 0.2 cm tissue Store specimens at room temperature Use cold pack for transport. Be sure cold pack is not in direct contact with specimen during transport Unstained Slides Send all slides within 6 weeks of cutting slides should be sent for HPV testing and 6-8 slides for JC, Kappa, Lambda, and EBER testing Precut slides from paraffin block in 5 micron sections and mounted on Poly-L-Lysinecoated or plus (+) slides Air dry; Do not oven dry Body Fluids Process fluids into formalin-fixed, paraffin-embedded cell blocks Immunophenotyping by Flow Cytometry Fresh tissue Bone marrow Peripheral blood 14

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