a b c periosteum parietal bone bone marrow dura periosteum suture mesenchyme osteogenic front suture mesenchyme 1
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1 coronary suture sagittal suture DOI: /ncb3139 a b c e parietal bone suture mesenchyme parietal bone bone marrow ura ura ura f parietal bone ura suture mesenchyme bone g ura osteogenic front suture mesenchyme osteogenic front ura Supplementary Figure 1 Anatomy an histology of craniofacial sutures. (a-c) MicroCT images of one-month-ol wil type mice. Each craniofacial bone is labele with a ifferent color. (-f) HE staining of the sagittal suture (), parietal bone (e) an coronal suture (f). Dotte lines outline the calvarial bones. (g) Schematic rawing of suture organization. Scale bars in panels a-c, 1mm; other scale bars, 100µm Macmillan Publishers Limite. All rights reserve
2 occipital a parietal b c interparietal parietal interparietal parietal zygomatic e f g h zygomatic pre Gli1-LacZ i j k l m pre n β-gal Sp7 o β-gal Runx2 basosphenoi basosphenoi p P8 p-a q P30 p-a p-b p-b Posterior Frontal Supplementary Figure 2 Gli1+ cells are etectable in the mesenchyme of most craniofacial sutures. (a-m) LacZ staining of craniofacial sutures of one-monthol Gli1-LacZ mice. Gli1+ cells are etectable in the mi-suture mesenchyme of the lamboi (a), interparietal-occipital (b), parietal- (c), zygomatic (), -zygomatic (e), -pre (f), - (g), - (h), -pre (i), nasal- (j), inter (k), basosphenoi- (l) an basosphenoi- (m) sutures. (n-o) Immunohistochemical staining of osteogenic ifferentiation markers Sp7 or Runx2 an lacz staining (βgal) of craniofacial sutures of one-month-ol Gli1- LacZ mice. Arrows inicate positive Sp7 or Runx2 signal. (p) Whole mount LacZ staining of the posterior suture of 8-ay-ol (P8) Gli1-LacZ pups. The two panels on the right (p-a, p-b ) are sections of the posterior an sagittal sutures an their positions are shown with the arrow an arrowhea. Asterisks inicate the suture mesenchyme. Dotte lines outline the bone surface. (q) LacZ staining of the posterior suture of one-month-ol Gli1- LacZ mice. Scale bar in panel p, 1mm; other scale bars, 100µm Macmillan Publishers Limite. All rights reserve
3 a b c 1 week post-inuction e f g h i j k l Gli1-Cre ERT2 ;R26Ttomato flox 1 month post-inuction m n Sp7 a b c e f g h i j k l m n Sp7 o Runx2 Supplementary Figure 3 Lineage tracing of Gli1+ cells in ault craniofacial sutures. Fluorescence imaging of sutures in Gli1- Cre ERT2 ;R26Ttomato fl mice one week (a-n) an one month (a -o ) after inuction at 1 month of age. Sutures visualize inclue the lamboi (a, a ), interparietal-occipital (b, b ), parietal- (c, c ), zygomatic (, ), -zygomatic (e, e ), -pre (f, f ), - (g, g ), - (h, h ), -pre (i, i ), nasal- (j, j ), inter (k, k ), basosphenoi- (l, l ) an basosphenoi- (m, m ). (n, n, o ) Immunostaining of Sp7 or Runx2 in the osteogenic front of Gli1-Cre ERT2 ;R26Ttomato fl mice. Arrowheas inicate Sp7+ or Runx2+ cells in the osteogenic front. Dotte lines outline the bone surfaces. Scale bars, 100µm Macmillan Publishers Limite. All rights reserve
4 Gli1-LacZ a 120% 100% b bone marrow Gli1+ cells suture Gli1+ cells 80% 60% 40% 98% 90% 90% 85% 85% 90% 90% 20% Gli1-Cre ERT2 ; R26Ttomato flox c 0% parietal occipital palatal basosphenoi Supplementary Figure 4 Gli1+ cells in the marrow also contribute to bone formation. (a) LacZ staining of the parietal bone of one-month-ol Gli1-LacZ mice. Arrows inicate positive signal. (b) Percentage of suture Gli1+ cells in the parietal,, occipital, ry, palatal, basosphenoi an bones of one-monthol Gli1-LacZ mice. Values are plotte as mean, n=5 samples. (c-) Visualization of Gli1+ cells in Gli1-Cre ERT2 ;R26Ttomato fl mice inuce at 1 month of age. Gli1+ cells are etectable in the marrow space of the basosphenoi bone (arrows in c). One month after inuction, osteocytes close to the bone marrow space are also labele (arrows in ), although bloo cells in the bone marrow are not. Scale bars, 100µm Macmillan Publishers Limite. All rights reserve
5 Rel. RNA level Rel. cell number Sagittal Sututre Incisor Rel. EU+ cell number Ctrl Smo flox/flox a c b c i k Gli1-Cre ERT2 ;Smo flox/flox ; Gli1-CreERT2 ; Smo R26ZsGreen flox flox/flox LacZ staining Alizarin Re e g h m n o p 3mo Ctrl 1mo Ctrl DTA f g h Gli1-Cre ERT2 ;DTA flox/flox ; R26ZsGreen flox j EU incorporation TUNEL assay Ctrl Smo flox/flox Ctrl Smo flox/flox Gli1-Cre ERT2 ;Smo flox/flox Gli1-Cre ERT2 ;Smo flox/flox EU EU TUNEL 1.2 incisor sagittal suture 0.6 EU EU TUNEL TUNEL Ctrl IHH 500ng/ml GDC0449 inhibitor ctrl Mutant ctrl Mutant l EU incorporation TUNEL TUNEL Ctrl IHH Inhibitor Ctrl IHH Inhibitor Ctrl IHH Inhibitor p=0.01 p=0.02 p=0.02 p=0.02 u v w x y Sagittal suture Parietal bone Palatal suture q r s 1cm z Sagittal suture Coronal suture t p=0.02 p=0.01 p=0.03 ALPase Runx2 Sp7 Osteocalcin Incisor Sagittal Sututre p=0.01 Supplementary Figure 5 Phenotypes of Smo ICKO an DTA mice. (a-h) MicroCT images of incisors of Smoothene flox/flox (control) an Gli1- Cre ERT2 ;Smoothene flox/flox (Smo ICKO) mice inuce at one month of age an analyze two months later. Arrows inicate normal calcifie tissue an arrowheas inicate isrupte calcifie tissue in sagittal (b, f) an cross (c-, g-h) sections. (i-l) HE staining of incisors in control (i, k) an Smo ICKO (j, l) mice. Normal an isrupte enamel an entin formation are inicate by the arrow an arrowhea, respectively. Asterisks inicate perioontal tissue efects. (m-o) EU incorporation an TUNEL assays in the incisors an sagittal sutures of control an Smo ICKO mice inuce at one month of age an analyze one month later. (n) Quantification of the relative numbers of EU+ cells. Values are plotte as mean ±SEM. Stuent s t-test was performe. n=4 mouse samples. (p) Lineage tracing analysis in the sagittal suture, parietal bone an palatal suture of Gli1-Cre ERT2 ;Smo fl/fl ;R26ZsGreen fl mice inuce at one month of age an analyze two months later. (q-x) LacZ an Alizarin Re staining of MSCs from the suture mesenchyme of one-month-ol Gli1-LacZ mice, either untreate (ctrl) or treate with IHH or GDC0449. (t) Quantitation of Eu incorporation an TUNEL assays of the same MSC cultures. Values are plotte as mean ±SEM an Stuent s t-test was performe. n=4 culture wells. (x) Real-time PCR of osteogenic ifferentiation markers incluing ALPase, Runx2, Sp7 an Osteocalcin of the same MSC cultures., ANOVA was performe an p values were inicate in the figure, n=4 samples. (y-z) Gli1-Cre ERT2 ;R26DTA fl/fl ;R26ZsGreen fl an control (Ctrl) mice were inuce at one month of age an analyze two months later. Fluorescently labele cells in the fuse sagittal an coronal sutures inicate cell ablation is not 100% efficient. Dotte lines outline the ental epithelium or bone. Scale bars in panels a-h, 1mm; scale bar in panel y, 1cm; other scale bars, 100µm Macmillan Publishers Limite. All rights reserve
6 a ura suture MSCs ura b ura IHH Gli1+ MSCs IHH osteogenic front ura Supplementary Figure 6 The suture mesenchyme provies an MSC niche for ault s. (a) Gli1+ MSCs within the suture mesenchyme give rise to the osteogenic front, an ura. These MSCs also give rise to the osteocytes either irectly in the osteogenic front region or inirectly through the or ura. (b) IHH secrete from the osteogenic front regulates the ifferentiation of Gli1+ MSCs in the suture mesenchyme Macmillan Publishers Limite. All rights reserve
Supplementary Figure 1: Signaling centers contain few proliferating cells, express p21, and
Supplementary Figure 1: Signaling centers contain few proliferating cells, express p21, and exclude YAP from the nucleus. (a) Schematic diagram of an E10.5 mouse embryo. (b,c) Sections at B and C in (a)
Supplementary Figure 1. EC-specific Deletion of Snail1 Does Not Affect EC Apoptosis. (a,b) Cryo-sections of WT (a) and Snail1 LOF (b) embryos at
Supplementary Figure 1. EC-specific Deletion of Snail1 Does Not Affect EC Apoptosis. (a,b) Cryo-sections of WT (a) and Snail1 LOF (b) embryos at E10.5 were double-stained for TUNEL (red) and PECAM-1 (green).
Figure S1. (A) Schematic diagram of dnrar transgene allele. (B) X-Gal staining of testis from
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Supplementary Figure 1. Expression of phospho-sik3 in normal and osteoarthritic articular cartilage in the knee. (a) Semiserial histological sections
Supplementary Figure 1. Expression of phospho-sik3 in normal and osteoarthritic articular cartilage in the knee. (a) Semiserial histological sections of normal cartilage were stained with safranin O-fast
Supplemental Figure 1. Western blot analysis indicated that MIF was detected in the fractions of
Supplemental Figure Legends Supplemental Figure 1. Western blot analysis indicated that was detected in the fractions of plasma membrane and cytosol but not in nuclear fraction isolated from Pkd1 null
Supplemental Figure 1. Egr1 expression in adult Achilles tendons. (A,B) Achilles tendons were isolated from 2 month-old Egr1 +/- mice and stained for
Supplemental Figure 1. Egr1 expression in adult Achilles tendons. (A,B) Achilles tendons were isolated from 2 month-old Egr1 +/- mice and stained for LacZ activity, which reflects Egr1 expression. (A)
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DOI: 10.1038/ncb2697 Figure S1 Cytokeratin 5 is a specific marker for basal and intermediate cells in all mouse prostate lobes. (a) Immunofluorescence staining showing co-localization of YFP with p63 in
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Supplementary Table 1. List of primers used in this study
Supplementary Table 1. List of primers used in this study Gene Forward primer Reverse primer Rat Met 5 -aggtcgcttcatgcaggt-3 5 -tccggagacacaggatgg-3 Rat Runx1 5 -cctccttgaaccactccact-3 5 -ctggatctgcctggcatc-3
SOPten flox/flox (KO) Pten flox/flox (WT) flox allele 6.0 kb. Pten. Actin. ! allele 2.3 kb. Supplementary Figure S1. Yanagi, et al.
s1 A Pten flox/flox () SOPten flox/flox () flox allele 6. kb B Pten flox/flox () SOPten flox/flox () Pten Actin! allele 2.3 kb Supplementary Figure S1. Yanagi, et al. A B BrdU BrdU positive cells ( ) 3
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1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Supplementary Figure 1. Kinetics of SnCs development in surgically-induced OA and effect of GCV-induced SnC clearance on OA disease progression
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Tcf21 MCM ; R26 mtmg Sham GFP Col 1/3 TAC 8W TAC 2W. Postn MCM ; R26 mtmg Sham GFP Col 1/3 TAC 8W TAC 2W
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Combination index (CI) Supplementary Figure 1 2. 1.5 1. Ishikawa AN3CA Nou-1 Hec-18.5...2.4.6.8 1. Fraction affected (Fa) Supplementary Figure 1. The synergistic effect of PARP inhibitor and PI3K inhibitor
Mitosis. Single Nano Micro Milli Macro. Primary. PCNA expression
a b c DAPI YFP CC3 DAPI YFP PCNA DAPI YFP ph3 DAPI YFP KI67 e 6 Mitosis f 1 PCNA expression %ph3 + /YFP + n= 63 87 61 3 13 8 n= 15 3 9 1 5 %PCNA+/YFP+ 8 6 Supplementary Figure 1. Proliferation/apoptosis
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Postn MCM Smad2 fl/fl Postn MCM Smad3 fl/fl Postn MCM Smad2/3 fl/fl. Postn MCM. Tgfbr1/2 fl/fl TAC
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a. Smo+/+ b. Smo+/+ 5.63 5.48 c. Lin- d. e. 6 5 4 3 Ter119 Mac B T Sca1 Smo+/+ 25 15 2 o BMT 2 1 5 * Supplementary Figure 1: Deletion of Smoothened does not alter the frequency of hematopoietic lineages
Specimen. Humeral Head. Femoral Head. Objective. Femoral Condyle (medial) Supplementary Figure 1
A B Specimen Humeral Head 2 1 µm 76 µm Femoral Head Objective Femoral Condyle (medial) Supplementary Figure 1 A Femoral Head Global Cell Density Superficial Cell Density Cell Number at 1 µm Nuclei /.1
IL-34 is a tissue-restricted ligand of CSF1R required for the development of Langerhans cells and microglia
Supplementary Figures IL-34 is a tissue-restricted ligand of CSF1R required for the development of Langerhans cells and microglia Yaming Wang, Kristy J. Szretter, William Vermi, Susan Gilfillan, Cristina
Supplementary material. Supplementary Figure legends
Supplementary material Supplementary Figure legends Supplementary Figure 1: Senescence-associated proliferation stop in response to oncogenic N-RAS expression Proliferation of NHEM cells without (ctrl.)
effects on organ development. a-f, Eye and wing discs with clones of ε j2b10 show no
Supplementary Figure 1. Loss of function clones of 14-3-3 or 14-3-3 show no significant effects on organ development. a-f, Eye and wing discs with clones of 14-3-3ε j2b10 show no obvious defects in Elav
SOM Husse et al. Supplementary online material. Synaptotagmin10-Cre, a driver to disrupt clock genes in the SCN
SOM Husse et al. Supplementary online material Synaptotagmin10-Cre, a driver to disrupt clock genes in the SCN Jana Husse, Xunlei Zhou, Anton Shostak, Henrik Oster and Gregor Eichele SOM Husse et al.,
Supplementary Figure 1. Deletion of Smad3 prevents B16F10 melanoma invasion and metastasis in a mouse s.c. tumor model.
A B16F1 s.c. Lung LN Distant lymph nodes Colon B B16F1 s.c. Supplementary Figure 1. Deletion of Smad3 prevents B16F1 melanoma invasion and metastasis in a mouse s.c. tumor model. Highly invasive growth
Supplementary Figure 1
Supplementary Figure 1 YAP negatively regulates IFN- signaling. (a) Immunoblot analysis of Yap knockdown efficiency with sh-yap (#1 to #4 independent constructs) in Raw264.7 cells. (b) IFN- -Luc and PRDs
Supplementary Information
Nature Immunology doi:1.138/ni.2477 Supplementary Information Capillary and arteriolar pericytes attract innate leukocytes exiting through venules and instruct them with pattern recognition and motility
Supplementary Figure 1. Properties of various IZUMO1 monoclonal antibodies and behavior of SPACA6. (a) (b) (c) (d) (e) (f) (g) .
Supplementary Figure 1. Properties of various IZUMO1 monoclonal antibodies and behavior of SPACA6. (a) The inhibitory effects of new antibodies (Mab17 and Mab18). They were investigated in in vitro fertilization
Supplemental Table 1. Primers used for RT-PCR analysis of inflammatory cytokines Gene Primer Sequence
Supplemental Table 1. Primers used for RT-PCR analysis of inflammatory cytokines Gene Primer Sequence IL-1α Forward primer 5 -CAAGATGGCCAAAGTTCGTGAC-3' Reverse primer 5 -GTCTCATGAAGTGAGCCATAGC-3 IL-1β
Nature Neuroscience: doi: /nn Supplementary Figure 1. Confirmation that optogenetic inhibition of dopaminergic neurons affects choice
Supplementary Figure 1 Confirmation that optogenetic inhibition of dopaminergic neurons affects choice (a) Sample behavioral trace as in Figure 1d, but with NpHR stimulation trials depicted as green blocks
Nature Medicine doi: /nm.2860
Supplemental Figure Legends Supplemental Figure 1: Hypomorphic expression of IFT88 results in olfactory signaling proteins no longer localizing to the ciliary layer. (a) ACIII localizes to the cilia and
Quantitative PPARγ expression affects the balance between tolerance and immunity
Quantitative PPARγ expression affects the balance between tolerance and immunity Ya-Hui Liu 1, Yau-Sheng Tsai 1,2,3, Shih-Chieh Lin 4, Nan-Shih Liao 5, Ming-Shiou Jan 6, Chung-Tiang Liang 7, Shih-Wen Hsu
Supplementary Figure 1. Generation of knockin mice expressing L-selectinN138G. (a) Schematics of the Sellg allele (top), the targeting vector, the
Supplementary Figure 1. Generation of knockin mice expressing L-selectinN138G. (a) Schematics of the Sellg allele (top), the targeting vector, the targeted allele in ES cells, and the mutant allele in
1.5 ASK1KO fed. fasted 16 hrs w/o water. Fed. 4th. 4th WT ASK1KO N=29, 11(WT), ,5(ASK1KO) ASK1KO ASK1KO **** Time [h]
![1.5 ASK1KO fed. fasted 16 hrs w/o water. Fed. 4th. 4th WT ASK1KO N=29, 11(WT), ,5(ASK1KO) ASK1KO ASK1KO **** Time [h]](/thumbs/87/97258189.jpg "1.5 ASK1KO fed. fasted 16 hrs w/o water. Fed. 4th. 4th WT ASK1KO N=29, 11(WT), ,5(ASK1KO) ASK1KO ASK1KO **** Time [h]")
7: 13: 19: 1: 7: 151117 a 151117 4th 4th b c RQ.95 KO.9.85.8.75.7 light dark light dark.65 7: 19: 7: 19: 7: Means ± SEM, N=6 RQ 1..9.8.7.6.6 KO CL (-) CL (+) ibat weight ratio (/body weight) [%].5.4.3.2.1
Supplementary Figures
Supplementary Figures Supplementary Figure 1 Characterization of stable expression of GlucB and sshbira in the CT26 cell line (a) Live cell imaging of stable CT26 cells expressing green fluorescent protein
Title: Smooth muscle cell-specific Tgfbr1 deficiency promotes aortic aneurysm formation by stimulating multiple signaling events
Title: Smooth muscle cell-specific Tgfbr1 deficiency promotes aortic aneurysm formation by stimulating multiple signaling events Pu Yang 1, 3, radley M. Schmit 1, Chunhua Fu 1, Kenneth DeSart 1, S. Paul
Figure S1. Reduction in glomerular mir-146a levels correlate with progression to higher albuminuria in diabetic patients.
Supplementary Materials Supplementary Figures Figure S1. Reduction in glomerular mir-146a levels correlate with progression to higher albuminuria in diabetic patients. Figure S2. Expression level of podocyte
Supplemental Data Tamoxifen administration to Vil-Scap- mice.
Supplemental Data FIGURE S1. Tamoxifen administration to Vil-Scap - mice. In the experiments shown in Fig. 1 to Fig. 5, tamoxifen (2 mg per dose) was dissolved in corn oil and administered by orogastric
Supplementary Figure 1. Spatial distribution of LRP5 and β-catenin in intact cardiomyocytes. (a) and (b) Immunofluorescence staining of endogenous
Supplementary Figure 1. Spatial distribution of LRP5 and β-catenin in intact cardiomyocytes. (a) and (b) Immunofluorescence staining of endogenous LRP5 in intact adult mouse ventricular myocytes (AMVMs)
Supplementary Information
Supplementary Information Astrocytes regulate adult hippocampal neurogenesis through ephrin-b signaling Randolph S. Ashton, Anthony Conway, Chinmay Pangarkar, Jamie Bergen, Kwang-Il Lim, Priya Shah, Mina
Shh signaling guides spatial pathfinding of raphespinal tract axons by multidirectional repulsion
ORIGINAL ARTICLE Cell Research (2012) 22:697-716. 2012 IBCB, SIBS, CAS All rights reserved 1001-0602/12 $ 32.00 www.nature.com/cr npg Shh signaling guides spatial pathfinding of raphespinal tract axons
Nature Neuroscience: doi: /nn Supplementary Figure 1. MADM labeling of thalamic clones.
Supplementary Figure 1 MADM labeling of thalamic clones. (a) Confocal images of an E12 Nestin-CreERT2;Ai9-tdTomato brain treated with TM at E10 and stained for BLBP (green), a radial glial progenitor-specific
Supplementary fig. 1. Crystals induce necroptosis does not involve caspases, TNF receptor or NLRP3. A. Mouse tubular epithelial cells were pretreated
Supplementary fig. 1. Crystals induce necroptosis does not involve caspases, TNF receptor or NLRP3. A. Mouse tubular epithelial cells were pretreated with zvad-fmk (10µM) and exposed to calcium oxalate
Supplemental Table S1. Primers used in qrt-pcr analyses. Supplemental Figure S1, related to Figure 4. Extracellular matrix proteins
Supplemental Material PDGFRb regulates craniofacial development through homodimers and functional heterodimers with PDGFRa Katherine A. Fantauzzo and Philippe Soriano Supplemental materials provided: Supplemental
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DOI: 10.1038/ncb3461 In the format provided by the authors and unedited. Supplementary Figure 1 (associated to Figure 1). Cpeb4 gene-targeted mice develop liver steatosis. a, Immunoblot displaying CPEB4
Nature Neuroscience: doi: /nn Supplementary Figure 1
Supplementary Figure 1 Atlas representations of the midcingulate (MCC) region targeted in this study compared against the anterior cingulate (ACC) region commonly reported. Coronal sections are shown on