ORIGINAL ARTICLE. Received Revised Accepted

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1 Bulletin of Environment, Phrmology nd Life Sienes Bull. Env.Phrmol. Life Si., Vol 4 [6] My 2015: Ademy for Environment nd Life Sienes, Indi Online ISSN Journl s URL: CODEN: BEPLAD Glol Impt Ftor Universl Impt Ftor ORIGINAL ARTICLE The Effets of Different levels of using Zin nno helted fertilizers nd humi id on Growth Prmeters nd on some qulity nd quntity Chrteristis of Mediinl Plnts of Svory 1 * Zhr Njfivf, 2 Noosheen Flhi, 3 Mrym Zre, 4 Sousn Ndi Bohloli, 5 Ali Rez Sirousmehr Corresponding uthor: zhr_njfivf@yhoo.om 1 College of Agriulture, The University of Zol, Zol, Irn. 2 College of Agriulture Mster of Siene in Field of Plnt Breeding, Kermnshh,Irn. 3 College of Agriulture, The University of Zol, Zol, Irn. 4 College of Agriulture, The University of Zol, Zol, Irn. 5 College of Agriulture, Zol University, Zol,Irn. ABSTRACT svory, memer of Lite fmily, is n nnul plnt. In order to investigte the effet of zin nno helted fertilizers folir pplition nd humi id on svory, n experiment ws rrnged on rndomized design in four replitions during the ropping sesons in the reserh greenhouse of the Institute of, University of zol, Irn). The tretments were humi id onentrtions in four levels (0, 0.5, 1, nd 1.5 on eh one 1000 m/liters wter) nd nno Zn helted fertilizer in four levels (0, 50, 100, nd 200 mg; on eh one t 1000 m/liters per wter) nd seond ftor inluded zin nno helted folir pplition in four levels, Zn1, ontrol, Zn2, 50mg zin nno helted fertilizers folir pplition per 1000m/ liter wter, Zn3; 100mg zin nno helted fertilizers folir pplition per 1000m/ liter wter nd Zn4, 200mg zin nno helted fertilizers folir pplition per 1000 liter wter. The results showed tht humi id, nno zn helted fertilizer nd the intertion of humi id nd nno Zn helted fertilizer hd signifint in 1 % proility level effets on Stem dimeter, Root length, root dry weight, root fresh weight, Phosphorus,Numer of leves per plnt, Chlorophyll ontent t (SPAD vlue), Essentil oil ontent t nd zin ontent.applition system Fertilizers effetive in improving qulitynd quntity hrterties nd the highest onentrtion of Stem dimeter, Root length, root dry weight, root fresh weight, Phosphorus,Numer of leves per plnt, Chlorophyll ontent t (SPAD vlue), Essentil oil ontent t nd zin ontent.were otined in mixture tretment. Keywords: nno fertilizer, Zin, Humi id, Svory Reeived Revised Aepted INTRODUCTION Prodution of mediinl plnts is minly under the irumstnes of sustinle griulturl system.in this system, mngement of environmentl prmeters is very ritil. By using orret nutritionl soures through humi id, nno Zn helted fertilizers quntittive nd qulittive yield of mediinl plnts n e mximized.im of In this study the effet of nno Zn helted fertilizer nd humi id on growth prmeters nd Essentil oil ontent t were investigted. Svory (Sturej hortensis L.) is n nnul plnt from fmily Lite (Lmiee). It is ntive to southern Europe nd entrl nd southwest of Asi ountries suh s Irn [1, 2]. Shoot prts of this plnt re frequently used s dditives for mny foods. This plnt is lso used in the trditionl mediine to tret musle pins, indigestion, potentil, nd infetion diseses [2]. Mjor oil onstituents of this plnt re thymol, rvrol, -terpene nd orneol [3]. Some iofertilizers re iroil inoulnts ontin living ells of miro-orgnism suh s teri, lge nd fungi whih my help plnt growth. Biologil tivities re mrkedly enhned y miroil intertions in the rhizosphere of plnts [4]. Suitle nd useful usge of different kind of fertilizers is the min wy for reformtion nd potentil of soil fertility nd inresing of rops yield [5]. Eh plnt needs to ertin fertilizers ording to its needs nd soil nlyze results. Also mroelements is the ritil elements for plnts; however, miroelements ply the importnt role in rop produtivity where it is used in low rte. Optimum plnt nutrition nd mximum yield is hieved when nutrient BEPLS Vol 4 [6] My P g e 2015 AELS, INDIA

2 Njfivf et l elements re ville for plnt during the growing seson [6]. Mentl syntheti heltes re reommending ording to their stility in soil, soluility in wter, sorption pility y plnt root or ording to soli ph nd plnt type [7]. Khzr iron nno helte Zin nd mngnese supplements hd the potentil role in iron fertilizer [8]. Seven elements of ville nutrients in nturl environment hve low neessity for plnt growth. Some of them re sored in tion form suh s iron, mngnese, opper nd zin nd some of them re sored in one form suh s Br, molydenum nd hlorine [9]. The dvntge of lef feeding or folir nutrients is tht when its fst effet is need it is ville for rnhes, leves or fruit. Some prts of plnt suh s fruit, needs more food suh s the lium. In erly spring when soil temperture is low the roots ouldn t sor nutrients so, elements suh s or nd zin re need for plnt. In some ses suh s when inomptily phenomenon y ddition of some mteril ourred in plnt root or miroorgnism s lef feeding gets more importnt [10] showed tht y using of zin sulphte, opper sulphte esides the inresing of grin yield, iron, zin nd opper onentrtion in grin inresed nd protein rte inresed from 6-10% to 14 perentges. Khlily et l. [11] indited tht folir pplition of miro elements suh s iron, zin, mngnese in oth shooting nd little efore the flowering stges inresed the yield nd yield omponents of orn silge. The min differene etween nno tehnology nd other tehnologies is in mteril nd strutures whih re used in this tehnology. Nono powders re mixture of prtiles with dimensions etween 1 to 10 nm. One of the most importnt pplitions of nnotehnology in griulture nd trends in wter nd soil siene is using nno fertilizers for plnt nutrition. Phlvn nd Kolli [12] indited tht miroelements suh s iron nd zin inresed the grin numer per spike nd 1000 grin weight, in ddition this elements onentrtion were inresed in grin. Tody due to the low onentrtion of mironutrients in whet grin tht is the min food of the people of Irn mny diseses, suh s kidney stones, nemi, ftigue nd gstrointestinl disorders re ommon [6]. Humi ompounds hve potentils return to inrese popultion of soil potentil, espeilly in the surfe lyer of root rhizospher tht rete sustnes whih stimulte plnt growth [13]. Humi id is produt ontins mny elements whih improve the soil fertility nd inrese the 57potentils of nutrient elements y holding them on minerl surfes nd, onsequently, ffet Plnt growth nd yield. Fertility, plnt physiology nd environmentl sienes, s the multiple roles plyed y these mterils n gretly improve plnt growth nd nutrient uptke [14, 15]. Mny investigtors reported tht, pplition of humi sustnes led to remrkle inrement in soil orgni mtter whih improve plnt growth nd inrese rop prodution [16-18].Thus, the min ojetive of this study ws to investigte the effets of different mounts of humi id nd nno zn helted fertilizers on the growth morphologil nd physiologil of Sturej hortensis.the im of the present study ws the effets of folir sprying of HA nd nno zn helted fertilizers either lone or in omintion on the growth, quntittive nd qulittive hrteristis of svory. MATERIALS AND METHODS The experiment ws rried out in the glsshouse of University of zol, Irn, in 2014yer. To investigte the effets of nno zin nd humi id nd on svory ftoril experiment ws rried out using ompletely rndomized design (CRD) with four replitions. The first ftor inluded The tretments were humi id onentrtions in four levels (0, 0.5, 1, nd 1.5 on eh one 1000 m/liters wter). And seond ftor inluded zin nno helted folir pplition in four levels, Zn1, ontrol, Zn2, 50mg zin nno helted fertilizers folir pplition per 1000m/ liter wter, Zn3; 100mg zin nno helted fertilizers folir pplition per 1000m/ liter wter nd Zn4, 200mg zin nno helted fertilizers folir pplition per 1000 liter wter. Soil nlysis The seeds of svory were sown in the pots ontining 2/5 soil, The pot mixture were tested efore pplying tretments nd the texture ws sndy lom with PH=7.20, orgni C=2.88%, totl (Tle1). Reord dt growth ondition nd mesure prmeters All of the tretments were spryed in four stges regulrly during growing seson with 15 dy intervls on the shoot of svory. The first spry pplied 28 dys fter sowing nd other pplied 43, 58 nd 73 dys fter sowing. Before flowering In order to mesure prmeters, 4 plnts were seleted rndomly from eh pot t efore flowering stge. Following prmeters were reorded for eh smple: Root length, Stem dimeter, root fresh weight nd root dry weight, zin ontent nd Content t Essentil oil The eril prts of svory were olleted t the Before flowering stge. Essentil nlysis Air-drying of plnt mteril ws performed in shdy ple t room temperture for 10 dys. Dried eril prts (20gr) were sujeted to hydro-distilltion of dried smple of shoots, using Clevenger-type pprtus over 3 hours. The essentil oil ws dried over nhydrous sodium sulfte nd then essentil oil BEPLS Vol 4 [6] My P g e 2015 AELS, INDIA

3 Njfivf et l ontent t (v/w) nd yield for eh plot were determined. The dt were sujeted to nlysis of vrine (ANOVA) using SAS omputer softwre nd mens ompred with Dunn s t 1% level of proility. RESULTS AND DISCUSSION This study indited tht the folir sprying of humi id nd nno Zn helted fertilizer hd signifint effet on the growth I otined results from the present reserh indited tht folir pplition of humi id nd nno Zn helted fertilizer t suitle onentrtions hd positive effets on the morphologil, physiologil nd gronomil trits of svory. The stimulted vlues of iohemil onstituents strengthened the role of the pplied humi id nd nno Zn helted fertilizer in the metolism of svory plnts. Moreover, nd humi id, Chelted zin fertilizer nd Intertions of humi id nd nno Zn helted fertilizer tretments resulted in the inrese of Stem dimeter, Root length, root dry weight, root fresh weight, Phosphorus, Numer of leves per plnt, Chlorophyll ontent t (SPAD vlue), Essentil oil ontent t nd zin ontent mounts in omprison with Control. Root length Results of vrine nlysis tle (tle2) indite tht effet of humi id nd nno Zn helted fertilizer nd the intertion effet of nno Zn helted fertilizer nd on Root length ws signifint in 1 % proility level. The highest ontent t of essentil oil ws oserved in the intertion of humi id nd nno zn helted fertilizer. The lowest ontent t of Stem dimeter, Root length, root dry weight, root fresh weight, Essentil oil ontent t nd zin ontent were oserved in ontrol tretments (Tle 3). Results showed tht the intertion humi id nd nno Zn helted fertilizer signifintly inresed nd Stem dimeter, Root length, root dry weight, root fresh weight, Essentil oil ontent t nd zin ontent t, nd Also, the mximum Root length( m) ws oserved t tretment the intertion of humi id nd nno Zn helted fertilizer, nd the minimum Root length ( m) nd ws relted to tretment of no humi id nd nno Zn helted fertilizer, nd no nno Zn helted fertilizer nd humi id, respetively (Tle3).The results indited tht the highest ( m) Root length ws otined y utiliztion of humi id (Tle4). The lowest ( m) Root length ws gined y no pplition of humi id (Tle4).The mximum Root length ws in N4 nd minimum Root length ws N1 with tretments respetively with m nd m (Tle 4). Figure 1 shows the results of the minimum mount of Root length ws otined y ontrol tretment (without humi id nd nno Zn helted fertilizer pplition) the highest Root length mong intertion effet levels ws reorded from H 4N 4 tretment (1.5 /l folir sprying of humi id nd 200 on eh one t 1000 m/liters per wter nno Zn helted fertilizer. Humi ids ould inrese the plnt nd root growth in proper dilutions[22].stephn (1994) indited visosity of 50 mg/l humi Aid use inresing length of whet root from 13.1 m to 20.2 m [23]. Other results in exmintion of humi Aid effet on growth of roof show tht most growth eginning is in 54 mg/l visosity of humi Aid, whih inresing root s sorption pity in the presene of humi Aid, whih e the ftor of growth inresing [24]. Some reports indited tht y onsumption of zin, log onentrtion of hemogloin in the knots, iron nd zin onentrtion in the root, tivity of redutse nitrte in the leves nd the onentrtion of nitrogen in the roots nd eril prts of the penut lso will inrese [26]. Root growth enhnement hs een ttriuted to improved soil struture, stimultion of soil miroflor, nd uxin-like effets [25]. Numer of leves per plnt Results of vrine nlysis tle (tle1) indite tht effet of humi id nd nno Zn helted fertilizer nd the intertion effet of nno Zn helted fertilizer nd on numer of leves per plnt ws signifint in 1 % proility level (Tle2). The highest (43 leves plnt-1) nd lowest (19 leves plnt- 1) of numer of leves per plnt ws gined y ontrol nd nd humi id sole, respetively (Tle3).In ddition, the gretest Numer of leves per plnt ws hieved in H4 fertilizer tretment with 38 the lowest Numer of leves per plnting H 1 tretment ws 26 (Tle4). The gretest Numer of leves per plntws relted to N 4 fertilizer tretment with 38 nd the lowest Numer of leves per plnt ws relted to N 1 fertilizer tretment with 25(Tle5). Figure 2 shows the results of the Minimum Numer of leves per plnt of the tretment without fertilizer or ontrol nd mximum Numer of leves per plnt of tretment were gined in H 4N 4. Adosslm et l., [19] showed tht folir pplition of Zn hd the most effetive influene s ompre with soil pplition of Zn on inresed of yield. Humtes re nturl orgni sustnes, high in humi id nd ontining most of known tre minerls essentil to the growth of plnt life [20]. Studies of the positive effets of humi sustnes on plnt growth hve demonstrted the importne of optimum minerl supply, independent of nutrition [21]. Root fresh weight nd root dry weight But there ws signifint humi id nd nno Zn helted fertilizer tretment nd Intertions of humi id nd nno Zn helted fertilizer tretments respetively with gretest nd lowest root fresh weight rte (tle2). The highest root fresh weight ws hieved in N4H4 y utiliztion the intertion of humi BEPLS Vol 4 [6] My P g e 2015 AELS, INDIA

4 Njfivf et l id nd nno Zn helted fertilizer tretment s gr nd the lowest root fresh weight ws hieved in N1H1 fertilizer tretment s 0.097gr (Tle3). The highest ( gr) root fresh weight ws otined y humi id nd lowest ( gr) root fresh weight ontent t ws gined y ontrol (without using of humi id fertilizer) (Tle4). Aording to the results of men omprison, the highest root fresh weight ws hieved in N4 fertilizer tretment with gr nd the lowest root fresh weight ws hieved in N1 fertilizer tretment with gr nd there ws signifint ll the pplied fertilizer tretments (Tle5).The highest root dry weight ws hieved in N4H4 y utiliztion the intertion of humi id nd nno Zn helted fertilizer tretment s gr nd the lowest root dry weight ws hieved in N1H1 fertilizer tretment s gr (Tle3). The highest root dry weight ws hieved in N4 fertilizer tretment s gr nd the lowest root dry weight ws hieved in N1 fertilizer tretment s gr (Tle5).The gretest root dry weight ws hieved in humi id fertilizer tretment with gr nd the lowest root dry weight ws hieved in indite tht humi id with 0.077gr (Tle 4). Results of showed tht (Figure 4,5), mximum root fresh weight nd root dry weight ontent t of tretment were otined in N 4H 4 nd N 3H 4 nd Also, the minimum mount root fresh weight nd root dry weight ws otined y ontrol tretment (without humi id nd nno Zn helted fertilizer pplition). Durnus et l [14] indited udding nd plnt dry weight s norml soyen deresed when pled on drought shok during seed filling. Adosslm et l., [19] showed tht folir pplition of Zn hd the most effetive influene s ompre with soil pplition of Zn on inresed of yield. Stem dimeter in ddition the highest Stem dimeter ws hieved in N4H4 fertilizer tretment with m nd the lowest Stem dimeter ws hieved in N1H1fertilizer tretment with m (tle 3).to study on the effet of humi id fertilizer in Stem dimeter indited tht the highest Stem dimeter for H4 tretment ws hieved in the highest humi id level nd the lowest Stem dimeter ws gined y no pplition of humi id (Tle 4).Aording to the results of men omprison, the highest Stem dimeter ws hieved in N4 nno Zn helted fertilizer tretment with mnd the lowest Stem dimeter ws hieved in N1 nno zn helted fertilizer tretment with m (Tle5) Results of showed tht (Figure 5) the highest Stem dimeter ontent t mong intertion effet levels ws reorded from H 4N 4 tretment (1.5 /l folir sprying of humi id nd 200 on eh one t 1000 m/liters per wter nno Zn helted fertilizer. Also, the minimum mount Stem dimeter ws otined y ontrol tretment H 1N 1 (without humi id nd nno Zn helted fertilizer pplition). Bhmnyre et l., [27] showed tht Folir pplition of Zn nd B hd positive effeted on Khzr vriety of rie nd the yield ws inresed rpidly nd nutrient defiieny ws ompensted. Mostfvi et l., [28] lso reported tht simultneous onsumption of Zn nd Mn led to the 6.8% inrese of whet plnt height ompred to ontrol tretment. Other results in exmintion of humi Aid effet on growth of roof show tht most growth eginning is in 54 mg/l visosity of humi Aid, whih inresing root s sorption pity in the presene of humi Aid, whih e the ftor of growth inresing [29]. Chlorophyll ontent t (SPAD vlue) The intertion effet of humi id nno Zn helted fertilizer nd effet of humi id nd nno Zn helted fertilizer on hlorophyll ontent t ws signifint in 1 % proility level (Tle2). The results indited tht the highest (12.05 SPAD vlue) hlorophyll ontent t ws otined y utiliztion the intertion of humi id nd nno Zn helted fertilizer nd the lowest (1.4 SPAD vlue) hlorophyll ontent t ws gined y no pplition of humi id nd nno Zn helted fertilizer (Tle3). Mximum Chlorophyll ontent t ws relted to the H4 tretment minimum ws lso relted to not-using-fertilizer tretments nd reprodutive stges (Tle4). The gretest Chlorophyll ontent ws relted to N4 fertilizer tretment with 9.24 nd lowest Chlorophyll ontent tws relted to N1 fertilizer tretment with 5.74 (Tle5). Results of showed tht (Figure 6) the highest Chlorophyll ontent t (SPAD vlue)mong intertion effet levels ws reorded from H 3N 4 tretment. Also, the minimum mount Stem dimeter ws otined y ontrol tretment. Hemntrnjn nd Gry [30] indited tht using Zn led to inreses in lef hlorophyll nd indol eti id, so photosynthesis will e improved nd then dry mter will e inresed. Humi id improves the physil, hemil nd iologil properties of the soil nd influenes plnt growth [31]. Nitrogen nd phosphorus re the most limiting nutrients to vegettive prodution ut their suffiient use y mjority of the smllholder frmers eome limiting due to their high osts. There re severl prolems whih re impeding the lne nd effiient use of fertilizers. They my e well ddressed y the pplition of humi id. It seems tht humi sustnes my influene oth respirtion nd photosynthesis [32].Humi sustnes re n importnt soil omponent euse they onstitute stle frtion of ron nd improve wter holding pity, ph uffering nd therml insultion [33]. Content Essentil oil BEPLS Vol 4 [6] My P g e 2015 AELS, INDIA

5 Njfivf et l The mximum ( m/lit) nd minimum ( m/lit) of Content t Essentil oil ws oserved y pplition 200 on eh one t 1000 m/liters per wter nd1/5 on eh one 1000 m/liters wter) of on. methnol with nno Zn helted fertilizer nd humi id to ontrol tretment, respetively (Tle3). Whih ws signifintly in 1 % proility level.qulittive nd quntittive nlysis of essentil oils hve een shown in (Tle2). The results of men omprison, the highest Essentil oil ontent t ws hieved in H4 fertilizer tretment with m/lit nd the lowest Essentil oil ontent t ws hieved in H1 with m/lit (Tle 4). In ddition, the gretest Essentil oil ontent t ws relted to N4 fertilizer tretment with m/litnd the lowest Essentil oil ontent t ws relted to N1 fertilizer tretment with m/lit(tle5). Figure 7 shows the results of the highest essentil oil ontent of otined for tretment H 4N 4 ompred with plnt (ontrol). Bgheri nd Mzherilgh, [34] reported tht the pplition of low onsumption elements Mn nd Zn n hve positive signifint effet on the growth nd hemil ompositions of Cuminum yminum essene nd the use of their mixture showed further effet. Addition of humi nd fulvi ids hs numerous profit nd griulturists ll over the world re epting humi nd fulvi ids s vitl prt of their fertilizer progrm. It n e pplied diretly to the plnt folige in liquid form or to the soil in the form of grnules lone or s fertilizer mix. Humi id is one of the mjor omponents of humus. Humtes re nturl orgni sustnes, high in humi id nd ontining most of known tre minerls essentil to the growth of plnt life [20]. Folir sprying is new method for rop feeding whih miro nd mro nutrients in form of liquid re used into leves [35]. Humi id (HA) is promising nturl resoure tht n e used s n lterntive to syntheti fertilizers to inrese rop prodution. It exerts either diret effet, suh s on enzymti tivities nd memrne permeility, or n indiret effet, minly y hnging the soil struture [28]. Phosphorus ontent Results of vrine nlysis tle (tle2)the intertion effet of humi id nno Zn helted fertilizer hd signifint (P<0.01) effet Phosphorus nd indite tht effet of humi id nd nno Zn helted fertilizer on Phosphorus ontent t ws signifint in 1 % proility level. Also, the mximum Phosphorus(0.1500mg.g-1) ws oserved t tretment the intertion of humi id nd nno Zn helted fertilizer, nd the minimum Phosphorus (0.1175mg.g-1) nd dry weight (0.16 mg.g-1) ws relted to tretment of no humi id nd nno Zn helted fertilizer, nd no nno Zn helted fertilizer nd humi id, respetively (Tle3).tht the gretest Phosphorus ontent t ws hieved in H4 fertilizer tretment with mg.g-1 nd the lowest Phosphorus ontent t ws relted to N4 fertilizer tretment, with mg.g-1(tle4).additionlly the gretest Phosphorus ontent t ws relted to N3, N4 fertilizer tretment, respetively with ( mg.g-1) ( mg.g-1)nd the lowest Phosphorus ontent t ws relted to N 1 fertilizer tretment with mg.g-1 (Tle5). Figure 8 shows the results of the Minimum Phosphorus ontent t of the tretment without fertilizer or ontrol nd mximum of Phosphorus ontent t tretments were gined in H 4N 4, H 4N 3, H 3N 3 H 3N 4, H 2N 4, H 2N 3. HA is suspension, sed on potssiumhumtes, whih n e pplied suessfully in mny res of plnt prodution s plnt growth stimulnt or soil onditioner for enhning nturl resistne ginst plnt diseses nd pests [36, 37], stimultion plnt growth through inresed ell division, s well s optimized uptke of nutrients nd wter, moreover, HA stimulted the soil miroorgnisms [38, 39]. When dequte humi sustnes re present within the soil, the requirement for nitrogen, phosphorus nd potssium fertilizer pplitions my e redued [39]. Humi id (HA) nd phosphorus pplitions inresed the growth nd growth prmeter of pepper seedling. The omined effets of HA nd P pplition ws more effetive on growth nd growth prmeter thn eh seprte effet. Humi id pplition signifintly inresed N, P, K, C, Mg, S, Mn nd Cu ontents of shoot of pepper seedling [40].The stimultory effets of humi sustnes hve een diretly orrelted with enhned uptke of mronutrients, suh s nitrogen, phosphorus; sulfur [42], nd mironutrients, tht is, Fe, Zn, Cu nd Mn [41]. Zin ontent The results indited tht the highest ( ppm) zin ontent ws otined y utiliztion the intertion of humi id nd nno Zn helted fertilizer (Tle 3). The lowest ( ppm)zin ontent ws gined y no pplition of humi id nd nno Zn helted fertilizer (Tle 3). And gretest zin ontent men ws hieved in H4 fertilizer tretment with ppm humi id nd lowest zin ontent ontent ws hieved in H1 with ppm(tle4).men omprison indited tht the gretest zin ontent plnt ws relted to N4 fertilizer tretment with ppm nd lowest zin ontent plnt ws relted to N1 fertilizer tretment with ppm (Tle 5). The mximum nd minimum of zin ontent t ws oserved y H4N4 tretment fertilizer or pplition 200 on eh one t 1000 m/liters per wter nd 1/5 on eh one 1000 m/liters wter) of on. methnol with nno Zn helted fertilizer nd humi id to ontrol tretment H1N1 (Figure 9). Severl ftors ffet on the sorle mount of zin in the penut ultivtion soils. These ftors re inluding puity of zin ontining minerls in the soils, presene of lkline ph, nd high mount of lium ronte nd light BEPLS Vol 4 [6] My P g e 2015 AELS, INDIA

6 weight of the soil texture in the penut fields (Pilevry et l., 2008).Beyond the issue of HA pplition rte, other importnt ftors my limit gronomi enefit from HA pplition to griulturl soils. In nutrient solution studies, plnt growth response to HS tended to pek t less thn 100 mg_l 1 [41].Ntive soil dissolved orgni mtter (DOM),whih n perform some of the sme funtions s pplied HA [42], mye present t suffiient onentrtion to minimize or negte ny enefit of pplied HA. Tle-1.The physil nd hemil properties of frm soil in depth 0-10m Depth 0-10 m Soil texture Snd Lom Cly (%) 8 Nitrogen (%) Silt (%) 48 P(ppm) Snd (%) 53 u(pmm) 1.34 ph 7.20 K (ppm) 150 E.C. (ds/m) 3.40 Fe(pmm) 9.86 orgni C %2.88 zn(pmm) 1.78 N=0.288%, ville P=26.74mg/kg, ville K=150 mg/kg nd EC=3.40 ds/m. Tle2- Result of nlysis vrine on studied hrteristis in svory Men squre root dry Root Stem Essentil oil weight(gr) length(m) dimeter ontent t(m/lit) Tretments df root fresh weight(gr) Zin ontent t (ppm) Humi id(a) ** 0.021** 49.79** 0.098** 1.77** 0.180** nnozehelted ** 0.019** 20.50** 0.182** 1.94** 0.947** fertilizer(b) A*B ** ** 1.51** 0.012** 0.055** 0.020** Error Cv(%) R-Squre ns= Non signifint, ** = p < 0.01, nd * = p < 0.05 Njfivf et l humi id 0 (without using humi id) Tle 3. Mens omprison of the min effets humi id of nd nno zn helted fertilizer tretments on morphophysiologil nd gronomil trits of svory (Sturej hortensis L.). nno Zn helted fertilizer 0 (without using nno Zn helted fertilize ) 50mg on eh one t 1000 m/liters wter root fresh weight gr l k root dry weight gr Root length m g f Numer of leves per plnt h g Stem dimeter Cm h g Chlorophyl l ontent t (SPAD vlue l j Content t Essentil oil m/lit h g phosphor ous (mg.g-1 ) d zin onte m h 100mg on eh one t 1000 m/liters wter j de e defg e f f 200mg on eh one t 1000 m/liters wter i de d d d e e 0/5 on eh one 1000 m/liters wter) 0 (without using nno Zn helted fertilize ). 50mg on eh one t 1000m/liters wter h ij e d 23.00g 35.00d fg de j h g e l j 100mg on ehone t 1000 m/liters wter fg de 35.50d de d de e 200 on eh one t 1000 m/liters wter f d 26.50f f on eh one 1000 m/liters wter 0 (without using nno Zn helted fertilize ) 50 on eh one t 1000 m/liters per wter 100 on eh one t 1000 m/liters per wter 200 on eh one t 1000 m/liters per wter i g e de d efg d de i g d f e k g e BEPLS Vol 4 [6] My P g e 2015 AELS, INDIA

7 1/5 on eh one 1000 m/liters wter) Tretmen t H1 H2 H3 H4 0 (without using nno Zn helted fertilize ) 50 on eh one t 1000 m/liters per wter 100 on eh one t 1000 m/liters per wter 200 on eh one t 1000 m/liters per wter d Men with the sme letters in eh olumn does hve signifint differene t the 1% level of proility. Dunn t test. Tle4. Mens omprison of the min effets humi id of tretments on morphophysiologil nd gronomil trits of svory (Sturej hortensis L.) root fresh weight gr Root dry weight gr Root length Cm Numer of leves per plnt Njfivf et l d d Stem dimeter d d d H1: Control (0without using nno Zn helted fertilize ) humi id H2: tretment (humi id (./5 on eh one 1000 m/liters wter) humi id H3: tretment (humi id(1 on eh one 1000 m/liters wter) humi id H4:tretment(humi id(1.5 on eh one 1000 m/liters wter) humi id *ws not signifint efg def Chlorophyll ontent t (SPAD vlue) d i d Essentil oil ontent t m/lit d e d phosphorou s (mg.g-1 ) Tle 5. Mens omprison of effets nno Zn helted fertilizer tretments on morphophysiologil nd gronomil trits of svory (Sturej hortensis L.). tretment Root fresh weight gr Root dry weight gr Root length m Numer of leves Stem dimeter Chlorophyll ontent t (SPAD Essentil oil ontent phosphorous (mg.g-1 ) Zin ontent ppm per vlue) m/lit pln i d zin ontent ppm d d d d d N1:ontrol(0without using nno Zn helted fertilize) N2:tretment 50mg on eh one t 1000 m/liters wter nno Zn helted fertilizer N3:tretment100mg on eh one t 1000 m/liters wter.nno Zn helted fertilizer N4:tretment 200mg on eh one t 1000 m/liters wter nno Zn helted fertilizer. signifint. ws not N1 N2 N3 N d d Figure 1 -Averge the intertion of humi id nd nno Zn helted fertilizer root length svory Morphophysiologil nd gronomil trits of svory (Sturej hortensiss L.). BEPLS Vol 4 [6] My P g e 2015 AELS, INDIA

8 Njfivf et l H1:Contro(0without using nno Zn helted fertilize ) humi id, H2:tretment(humi id (./5 on eh one 1000 m/liters wter) humi id,h3:tretment (humi id(1 on eh one 1000 m/liters wter) humi id, H4:tretment(humi id(1.5 on eh one 1000 m/liters wter) humi id.n1:ontrol(0without using nno Zn helted fertilize), N2:tretment 50mg on eh one t 1000 m/liters wter nno Zn helted fertilizer N3:tretment100mg on eh one t 1000 m/liters wter.nno Zn helted fertilizer, N4:tretment 200mg on eh one t 1000 m/liters wter nno Zn helted fertilizer. Figure 2-Averge the intertion of humi id nd nno Zn helted fertilizer numer of leves per plnt svory Morphophysiologil nd gronomil trits of svory (Sturej hortensis L.) ). H1:Contro(0without using nno Zn helted fertilize ) humi id,h2:tretment(humi id (./5 on eh one 1000 m/liters wter) humi id,h3:tretment (humi id(1 on eh one 1000 m/liters wter) humi id,h4:tretment(humi id(1.5 on eh one 1000 m/liters wter) humi id.n1:ontrol( (0without using nno Zn helted fertilize), N2:tretment 50mg on eh one t 1000 m/liters wter nno Zn helted fertilizer N3:tretment100mg on eh one t 1000 m/liters wter.nno Zn helted fertilizer, N4:tretment 200mg on eh one t 1000 m/liters wter nno Zn helted fertilizer. Figure 3,4- Averge the intertion of humi id nd nno Zn helted fertilizer root fresh weight nd root dry weight svory Morphophysiologil nd gronomil trits of svory (Sturej hortensis L.). H1:Contro(0without using nno Zn helted fertilize ) humi id,h2:tretment(humi id (./5 on eh one 1000 m/liters wter) humi id,h3:tretment (humi id(1 on eh one 1000 m/liters wter) humi id,h4:tretment(humi id(1.5 on eh one 1000 m/liters wter) humi id,n1:ontrol( (0without using nno Zn helted fertilize), N2:tretment 50mg on eh one t 1000 m/liters wter nno Zn helted fertilizer N3:tretment100mg on eh one t 1000 m/liters wter.nno Zn helted fertilizer, N4:tretment 200mg on eh one t 1000 m/liters wter nno Zn helted fertilizer. BEPLS Vol 4 [6] My P g e 2015 AELS, INDIA

9 Njfivf et l Figure 5-Averge the intertion of humi id nd nno Zn helted fertilizer stem dimeter svory Morphophysiologil nd gronomil trits of svory (Sturej hortensis L.). H1:Contro(0without using nno Zn helted fertilize ) humi id,h2:tretment(humi id (./5 on eh one 1000 m/liters wter) humi id,h3:tretment (humi id(1 on eh one 1000 m/liters wter) humi id,h4:tretment(humi id(1.5 on eh one 1000 m/liters wter) humi id,n1:ontrol (0without using nno Zn helted fertilize), N2:tretmentt 50mg on eh one t 1000 m/liters wter nno Zn helted fertilizer N3:tretment100mg on eh one t 1000 m/liters wter. nno Zn helted fertilizer, N4:tretment 200mg on eh one t 1000 m/liters wter nno Zn helted fertilizer. Figure 6- Averge the intertionn of humi id nd nno Zn helted fertilizer hlorophyll ontent t svory Morphophysiologil nd gronomil trits of svory (Sturej hortensiss L.). H1:Contro(0without using nno Zn helted fertilize ) humi id,h2:tretment(humi id (0/5 on eh one 1000 m/liters wter) humi id,h3:tretment (humi id(1 on eh one 1000 m/liters wter) humi id,h4:tretment(humi id(1.5 on eh one 1000 m/liters wter) humi id. N1:ontrol(0without using nno Zn helted fertilize), N2:tretmentt 50mg on eh one t 1000 m/liters wter nno Zn helted fertilizer N3:tretment100mg on eh one t 1000 m/liters wter.nno Zn helted fertilizer, N4:tretment 200mg on eh one t 1000 m/liters wter nno Zn helted fertilizer. Figure 7- Averge the intertionn of humi id nd nno Zn helted fertilizer ontent t essentil oil svory Morphophysiologil nd gronomil trits of svory (Sturej hortensiss L.). H1:Contro(0without using nno Zn helted fertilize ) humi id,h2:tretment(humi id (./5 on eh one 1000 m/liters wter) humi id,h3:tretment (humi id(1 on eh one 1000 m/liters wter) humi id,h4:tretment (humi id(1.5 on eh one 1000 m/liters wter) humi id. N1:ontrol(0without using nno Zn helted fertilize), N2:tretment 50mg on eh one t 1000 m/liters wter nno Zn helted fertilizer N3:tretment100mg on BEPLS Vol 4 [6] My P g e 2015 AELS, INDIA

10 Njfivf et l eh one t 1000 m/liters wter.nno Zn helted fertilizer, N4:tretment 200mg on eh one t 1000 m/liters wter nno Zn helted fertilizer. Figure 8- Averge the intertionn of humi id nd nno Zn helted fertilizer phosphorus ontent t svory Morphophysiologil nd gronomil trits of svory (Sturej hortensiss L.). H1:Contro(0without using nno Zn helted fertilize ) humi id,h2:tretment(humi id (./5 on eh one 1000 m/liters wter) humi id,h3:tretment (humi id(1 on eh one 1000 m/liters wter) humi id,h4:tretment(humi id(1.5 on eh one 1000 m/liters wter) humi id. N1:ontrol( (0without using nno Zn helted fertilize), N2:tretment 50mg on eh one t 1000 m/liters wter nno Zn helted fertilizer N3:tretment100mg on eh one t 1000 m/liters wter. Nno Zn helted fertilizer, N4:tretment 200mg on eh one t 1000 m/liters wter nno Zn helted fertilizer. Figure 9-Averge the intertion of humi id nd nno Zn helted fertilizer zin ontent svory Morphophysiologil nd gronomil trits of svory (Sturej hortensiss L.). H1:Contro(0without using nno Zn helted fertilize ) humi id,h2:tretment(humi id (./5 on eh one 1000 m/liters wter) humi id,h3:tretment (humi id(1 on eh one 1000 m/liters wter) humi id,h4:tretment(humi id(1.5 on eh one 1000 m/liters wter) humi id. N1: ontrol (0without using nno Zn helted fertilize), N2:tretmentt 50mg on eh one t 1000 m/liters wter nno Zn helted fertilizer N3:tretment100mg on eh one t 1000 m/liters wter. nno Zn helted fertilizer, N4:tretment 200mg on eh one t 1000 m/liters wter nno Zn helted fertilizer. RECOMMENDATIONS 1. It is reommended tht other humi id nd nno Zn helted fertilizer re exmined nd tests re reported in the ples nd yers. 2. Aording to the tre elements shortge rte in the ultivting soils nd humns need to these elements it is suggested tht neessry mironutrients e provide for the plnt in dequte mounts. 3. Sine zin nno Zn helted fertilizer will inrese the humi id doption nd thus inreses the yield elements, it is reommended tht two fertilizer tretment to humi id nd nno Zn helted fertilizer eing utilized with suitle rtes longside eh other due to their positive effets. REFERENCES 1. Boskdy,MH., Aslni, M.R, Kini, S. (2006). Relxnt effet of Thymus vulgris on guine-pig trhel hins nd its possile mehnism(s). J. Phytotherpy Res. 20: Gontru, L., Plnder, S., Simndi, B.(2008). Investigtion of sturej hortensis l. s possile soure of nturl ntioxidnts.hungrin J. Industril Chem.Veszprem. 36: BEPLS Vol 4 [6] My P g e 2015 AELS, INDIA

11 Njfivf et l 3. Boskdy, M.H., Aslni, M.R., Mnsuri, F., Amery, S. (2007). Relxnt effet of Sturej hortensis on guine pig trhel hins nd its possile mehnism(s).dru., 15: Tilk, K.V., Reddy, B.S.(2006). B. ereus nd B. irulns novel inoulnts for rops. Curr Si., 5: Hossein, Tlei, G.h. (2012). Effet of io nd hemil fertilizers on yield nd yield omponents of umin (imin imio).ms thesis.univ. Shhed, Tehrn, Irn. 6. Mlkooti, M.J., Ttei, S.J.(1998). Plnt feeding y folir pplition.8th tehnil j. Agr.Edut. Pul. Krj.Irn. 7. Vnluit. B., Boxm, R., (1981). Qulity hek of iron heltes pplied to ornmentl shrus on sphgnum pet. J. Hort. Si., 56: Khje, Poor, M.R.(1998). Priniples of Agriulture, Seond Edition, pulished y Jhd Dneshghi, Univ. Isfhn. 9. Khlj, H., Lffi, Hossein, Adi M.R., Nzrn, M.H., Sheini, K., Rzzi, A. (2008). Applition of nno-orgni fertilizer, folir fertilizer nd helted iron on the pple nd per yield.pp Mlkooti, M.J., Lotf, A.(1998). Effet of zin on inresing of quntitive nd qulity of produtions. Krj, Irn. 11. Khlil, Mhhleh, J., Rez, doost, S., Roshdi, M., (2002). Effet of folir mironutrient elements on quntittive nd qulittive hrteristis of 704 orn silge in the Khoy region.ninth Congr. Soil Si.,Tehrn. Irn. 12. Phlvn, rd., M., Pessrkli, M., (2009). Response of Whet Plnt to Zin, Iron nd Mngnes Applitions nd Uptke nd Conentrtion of Zin, Iron nd Mngnes in Whet Grins. Comm. Soil Si. Plnt Anlys., (40): Awd, E. M.(2002).Effet of ompost nd some (iofertilizers on growth, yield nd gulity of ptto rop solnum tuerosum L.).J.Agri. Si. Mnsour Univ., 27: Dursun, A., Guven, I., Turn, M. ( 2002). Effet of different levels of humi id on seeding groth nd Mro-nd Miro-nutrient ontent ts of tomto nd eggplnt. ACTA Agrootni., 56: Pksoy, M., Turkmen, O., Dursun, A. (2010).Effet of Potssium nd humi rid on emergene growth nd nutrient ontent ts of okr ( Alelmoshus esulentus L.)sedling under slin soil onditions. Afr.J.Biotehnol.,9: Pdem,H., A.Ol.(1999). 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The olletion of rtiles for the 1st Ntionl Conferene of Cumin.Islmi Azd University of Cumin.pp BEPLS Vol 4 [6] My P g e 2015 AELS, INDIA

12 Njfivf et l 35. Nsiri, Y., Zeht,Slmsi, S., Nsrullhzdeh, S., Njfi, N., Ghssemi,Golezni, K.(2010).Effets of folir pplition of mironutrients (Fe nd Zn) on flower yield nd essentil oil of hmomile (Mtriri hmomill L.). Journl of Mediinl Plnts Reserh. 4(17): Sheuerell, S.J., Mhffee, W.H.(2006). Vriility Assoited with Suppression of Gry Mold (Botrytis inere) on Gernium y Folir Applitions of Nonerted nd Aerted Compost Tes. Plnt Dis., 90: Atiyeh, R.M., Lee, S., Edwrds, C.A., Arnon, N.Q., Metzger, J.D.( 2002). The influene of humi ids derived from erthworms- proessed orgni wstes on plnt growth. Bioresoure Tehnology., 84: Chen, Y., Clpp, C. E., Mgen, H., ClineV. W. (1999). Stimultion of plnt growth y humi sustnes: Effets on iron vilility. In: Ghour, EA, Dvies G. (eds.), Understnding humi sustnes: Advned methods, properties nd pplitions. Royl Soiety of Chemistry, Cmridge, UK.p Pettit, R. E. (2004). Orgni mtter, humus, humte, humi id, fulvi id nd humin: their importne in soil fertility nd plnt helth [Online]. Aville t Mesut, K., Cimrin, Önder, T., Metin, T., Buru, T. (2010). Phosphorus nd humi id pplition llevite slinity stress of pepper seedling. Afrin Journl of Biotehnology., 9(36): Chen, Y., Mgen, H.,Clpp, C.E.(2001). Plnt growth stimultion y humi sustnes nd their omplexes with iron. Proeedings of Interntionl Fertilizer Soiety.Isrel. pp Chen, C.R., Xu, Z.H, Mthers, N.J. (2004). Soil ron pools in djent nturl nd plnttion forests of sutropil Austrli. Soil Siene Soiety of Ameri Journl 68: Pilevry, Khommy, R., Sfrzde, Vishkii, M.N., Sjedi, N.A., Rsouli, M., nd Mordi.M.(2008). Amounts of methnol nd zin on quntittive nd qulittive hrteristis of the Giln penuts.new findings in the Journl of Agriulture, seond yer (4): CITATION OF THIS ARTICLE Zhr N, Noosheen F, Mrym Z, Sousn Ndi B, Ali Rez S. The Effets of Different levels of using Zin nno helted fertilizers nd humi id on Growth Prmeters nd on some qulity nd quntity Chrteristis of Mediinl Plnts of Svory. Bull. Env.Phrmol. Life Si., Vol 4 [6] My 2015: BEPLS Vol 4 [6] My P g e 2015 AELS, INDIA