OIE Reference Laboratory Reports Activities
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1 OIE Reference Laboratory Reports Activities Activities in 2015 This report has been submitted : :50:00 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Highly and low pathogenic avian influenza Address of laboratory: Suedufer Greifswald Insel Riems GERMANY Tel.: Fax: address: timm.harder@fli.bund.de Website: Name (including Title) of Head of Laboratory (Responsible Official): Prof. Dr. Timm C. Harder Name (including Title and Position) of OIE Reference Expert: Prof. Dr. Timm C. Harder Which of the following defines your laboratory? Check all that apply: Governmental OIE Reference Laboratory Reports Activities,
2 ToR 1: To use, promote and disseminate diagnostic methods validated according to OIE Standards 1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as disease diagnosis, screening of animals for export, surveillance, etc.? (t for quality control, proficiency testing or staff training) Diagnostic Test Indicated in OIE Manual (/) Total number of test performed last year Indirect diagnostic tests Nationally Internationally ELISA Hemagglutination inhibition Direct diagnostic tests Nationally Internationally RT-qPCR RT-PCR Sequencing Virus isolation/egg IVPI 2 0 ToR 2: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease. 2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE? 3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents to OIE Member Countries? 2 OIE Reference Laboratory Reports Activities, 2015
3 Type of reagent available Related diagnostic test Produced/ provide Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg). of recipient OIE Member Countries Region of recipients Viral RNA PCR 4 x 1 3 x 1 2 Africa Americas Asia and Pacific Europe Middle East Antiserum ELISA; HI 27 x 0,5 5 x 0,5 1 Africa Americas Asia and Pacific Europe Middle East Viral Antigen HI 138 x 1 5 x 1 1 Africa Americas Asia and Pacific Europe Middle East Infectious virus PCR, HI, IVPI 4 x 1 9 x 1 4 Africa Americas Asia and Pacific Europe Middle East 4. Did your laboratory produce vaccines? 5. Did your laboratory supply vaccines to OIE Member Countries? ToR 3: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases 6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease? OIE Reference Laboratory Reports Activities,
4 7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? Name of the new test or diagnostic method or vaccine developed Description and References (Publication, website, etc.) Riems-Influneza A Virus Typing Assay ("RITA") RT-qPCR based microarray for differentiation of influenza A virus subtypes. Manuscript in preparation. SYBR Green based genotyping tool for Egyptian H9N2 and H5N1 viruses Naguib et al., PMID: ToR 4: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries 8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? Name of OIE Member Country seeking assistance Date (month). samples received for provision of diagnostic support. samples received for provision of confirmatory diagnoses EGYPT 1, 2, UNITED ARAB EMIRATES Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? Name of the OIE Member Country receiving a technical consultancy Purpose How the advice was provided KOREA (REP. OF) Management of AIV in wild birds Seminar for 2 days ToR 5: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations 10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? 4 OIE Reference Laboratory Reports Activities, 2015
5 ToR 6: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases 11. Did your Laboratory collect epizootiological data relevant to international disease control? 12. Did your laboratory disseminate epizootiological data that had been processed and analysed? 13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: 9 1: Harder TC, Buda S, Hengel H, Beer M, Mettenleiter TC. Poultry food products- a source of avian influenza virus transmission to humans? Clin Microbiol Infect Dec 11. pii: S X(15) doi: /j.cmi [Epub ahead of print] Review. PubMed PMID: : Naguib MM, Kinne J, Chen H, Chan KH, Joseph S, Wong PC, Woo PC, Wernery R, Beer M, Wernery U, Harder TC. Outbreaks of highly pathogenic avian influenza H5N1 clade c in hunting falcons and kept wild birds in Dubai implicate intercontinental virus spread. J Gen Virol Sep 7. doi: /jgv [Epub ahead of print] PubMed PMID: : Naguib MM, Arafa AS, El-Kady MF, Selim AA, Gunalan V, Maurer-Stroh S, Goller KV, Hassan MK, Beer M, Abdelwhab EM, Harder TC. Evolutionary trajectories and diagnostic challenges of potentially zoonotic avian influenza viruses H5N1 and H9N2 co-circulating in Egypt. Infect Genet Evol Aug;34: doi: /j.meegid Epub 2015 Jun 3. PubMed PMID: : Fereidouni SR, Starick E, Ziller M, Harder TC, Unger H, Hamilton K, Globig A. Sample preparation for avian and porcine influenza virus cdna amplification simplified: Boiling vs. conventional RNA extraction. J Virol Methods Sep 1;221:62-7. doi: /j.jviromet Epub 2015 Apr 27. PubMed PMID: : Harder T, Maurer-Stroh S, Pohlmann A, Starick E, Höreth-Böntgen D, Albrecht K, Pannwitz G, Teifke J, Gunalan V, Lee RT, Sauter-Louis C, Homeier T, Staubach C, Wolf C, Strebelow G, Höper D, Grund C, Conraths FJ, Mettenleiter TC, Beer M. Influenza A(H5N8) Virus Similar to Strain in Korea Causing Highly Pathogenic Avian Influenza in Germany. Emerg Infect Dis May;21(5): doi: /eid PubMed PMID: ; PubMed Central PMCID: PMC : Schneider-Bühl L, Polley B, Süss-Dombrowski C, Müller S, Harder T, Hoferer M, Sting R. [Detection of the new influenza A subtype H1pdmN2 in a pig holding with severe respiratory symptoms]. Berl Munch Tierarztl Wochenschr Jan-Feb;128(1-2):20-3. German. PubMed PMID: : Arafa AS, Naguib MM, Luttermann C, Selim AA, Kilany WH, Hagag N, Samy A, Abdelhalim A, Hassan MK, Abdelwhab EM, Makonnen Y, Dauphin G, Lubroth J, Mettenleiter TC, Beer M, Grund C, Harder TC. Emergence of a novel cluster of influenza A(H5N1) virus clade with putative human health impact in Egypt, 2014/15. Euro Surveill Apr 2;20(13):2-8. PubMed PMID: : Rahn J, Hoffmann D, Harder TC, Beer M. Vaccines against influenza A viruses in poultry and swine: Status and future developments. Vaccine May 15;33(21): doi: /j.vaccine Epub 2015 Mar 31. Review. OIE Reference Laboratory Reports Activities,
6 PubMed PMID: Highly and low pathogenic avian influenza - Timm C. Harder - germany 9: Heider A, Mochalova L, Harder T, Tuzikov A, Bovin N, Wolff T, Matrosovich M, Schweiger B. Alterations in hemagglutinin receptor-binding specificity accompany the emergence of highly pathogenic avian influenza viruses. J Virol May;89(10): doi: /JVI Epub 2015 Mar 4. PubMed PMID: ; PubMed Central PMCID: PMC b) International conferences: 10 EFSA-Conference on gaps in influenza virus research, January, Parma, Italy Yogyakarta, Indonesia: BTSF Training, Februaray Egypt, Cairo: Vaccine usage against influenza in Egypt, Me-Vac Symposium, March Egypt, Cairo: Control of avian influenza in Egypt, Boehringer Symposium, vember Athens, GA, US: Internation Avian Influenza Symposium; invited speaker and session manager Kalmar, Sweden: Avian influenza in wild birds, May Budapest, Hungary: Meeting of the European national influenza reference laboratories Vienna, Austria: Viral dynamics in influenza, October. c) National conferences: 5 Montabaur, DE: ATF Symposium: Viral dynamics in influenza Hannover, DE: Geflügelfachgespräch, H5N8 outbreaks in Germany, March Berlin, DE: DVG Tierseuchentagung, Overview on AIV in Germany Erfurt, DE: Deutscher Influenzatag, Animal influenza in germany Greifswald, DE: Riemser Diagnostiktage, Diagnosis of animal influenza d) Other: (Provide website address or link to appropriate information) 0 ToR 7: To provide scientific and technical training for personnel from OIE Member Countries To recommend the prescribed and alternative tests or vaccines as OIE Standards 14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries? a) Technical visits: 2 b) Seminars: 1 c) Hands-on training courses: 1 d) Internships (>1 month): 1 6 OIE Reference Laboratory Reports Activities, 2015
7 Type of technical training provided (a, b, c or d) Country of origin of the expert(s) provided with training. participants from the corresponding country a, BTSF, Indonesia Singapore 2 a, BTSF, Indonesia Malyasia 2 a, BTSF, Indonesia Bhutan 1 a, BTSF, Indonesia Indonesia 15 a, BTSF, Indonesia Philippines 5 a, BTSF, Indonesia Thailand 4 a, BTSF, Indonesia Myanmar 2 a, BTSF, Indonesia Nepal 2 a, BTSF, Indonesia Cambodia 2 a, BTSF, Indonesia Laos 2 a, IAEA Bioinformatics, Vienna Ethiopia 2 a, IAEA Bioinformatics, Vienna Kenya 1 a, IAEA Bioinformatics, Vienna Mali 1 a, IAEA Bioinformatics, Vienna Mozambique 1 a, IAEA Bioinformatics, Vienna Namibia 1 a, IAEA Bioinformatics, Vienna Nepal 1 a, IAEA Bioinformatics, Vienna Senegal 1 a, IAEA Bioinformatics, Vienna Tanzania 1 a, IAEA Bioinformatics, Vienna Zambia 1 b, Poland, AIV seminar Poland 60 c, Laboratory training at FLI Botswana 1 d, PhD at FLI Egypt 1 ToR 8: To maintain a system of quality assurance, biosafety and biosecurity OIE Reference Laboratory Reports Activities,
8 relevant for the pathogen and the disease concerned 15. Does your laboratory have a Quality Management System certified according to an International Standard? Quality management system adopted ISO 17025:2005 Certificate scan (PDF, JPG, PNG format) Akkreditierung_2015.pdf 16. Is your laboratory accredited by an international accreditation body? 17. Does your laboratory maintain a biorisk management system for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2014, Chapter 1.1.3a) ToR 9: To organise and participate in scientific meetings on behalf of the OIE 18. Did your laboratory organise scientific meetings on behalf of the OIE? 19. Did your laboratory participate in scientific meetings on behalf of the OIE? Title of event Date (mm/yy) Location Role (speaker, presenting poster, short communications) Title of the work presented WHO PCR technical group June Geneva Speaker Overview on new methods in animal influenza diagnosis ToR 10: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results 20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease? 8 OIE Reference Laboratory Reports Activities, 2015
9 21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests? Purpose of the proficiency tests: 1 Role of your Reference Laboratory (organiser/ participant). participants Participating OIE Ref. Labs/ organising OIE Ref. Lab. OFFLU AI PT group Participant 15 Ames, Iowa, US 1 validation of a diagnostic protocol: specify the test; quality control of vaccines: specify the vaccine type, etc. 22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest? Title of the project or contract Scope Name(s) of relevant OIE Reference Laboratories AIV PT ring trials PT testing 9 HPAIV diagnosis in Africa Improved diagnostics 1 ToR 11: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results 23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease? te: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: see point 1.3 ToR 12: To place expert consultants at the disposal of the OIE 24. Did your laboratory place expert consultants at the disposal of the OIE? OIE Reference Laboratory Reports Activities,
10 25. Additional comments regarding your report: 10 OIE Reference Laboratory Reports Activities, 2015
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