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1 Paz pre-treatment g Paz g ev mount of ascites (m) g ev Suppementa Figure 1. ffect of versus g-term anti-angiogenic therapy. Mean aggregate tumor weight () and number of tumor nodues () of tumors induced by intraperitea injecti of Hey8 human ovarian cancer ces into nude mice after indicated treatments. Number of tumor nodues () and amount of ascites in m () of 774 intraperitea tumors after or g-term treatment with bevacizumab. (-) n=8-1 mice per group. P <.1, P <.1, P <. (1-way NOV foowed by a Tukey s mutipe comparis post-hoc test in -). veraged data are presented as the mean ±SM.
2 Pateets (GPIb) Vesses (31) Merge Pateets (GPIb) Vesses (31) Merge umina % 6P+ % 41/61+ (active form) R R PLT cancer ces thrombin resting abumina % poptotic ces normoxia normoxia + pts hypoxia hypoxia + pts Invasi (no. of ces) 1 6 normoxia normoxia + pts hypoxia hypoxia + pts Suppementa Figure. Pateet infitrati in vivo and pateet effect cancer ces in vi. () Higher magnificati of respresentative tumor sectis showing pateets attached to the vesse wa and at the point of extravasati (white arrowheads) or extravasated (white arrows) into tumor microenvirment. Pateets and tumor vesses were stained with antibodies against GPIb and 31, respectivey. Representative pictures of tumors from at east mice per group. () ectr microscopy picture of a pateet (PLT - back dashed ine) extravasating between two endotheia ces (, red dashed ine). R=red bood ce. Scae bar 1μm. Representative picture of tumors from at east mice. () vauati of P-seectin and 41/61 (active form) surface expressi in GPIbβ-positive pateets as evidence of pateet activati by cancer ces. Thrombin stimuati (.U/m) of pateets was used as positive c (n=3). () Number of apoptotic SKOV3 ovarian cancer ces (in %) under normoxic or hypoxic cditis with or without the additi of mii pateets (n=3). () Indirect effect of pateets SKOV3 invasiveness under normoxic and hypoxic cditis (no. of invaded ces, n=3). P <.1, P <.1, P <. (1-way NOV foowed by a unnett s mutipe comparis post-hoc test in ; two-taied Student s t-test in -). veraged data are presented as the mean ±SM.
3 1. direct. indirect reeasate 6 n YFP-abeed pateets N 8 4 ns fu p p ts ts tra P tra ns fu si P 1.. % Proiferati si o Pateet counts (x 1^6 /ml) 6 4 P + P w ith dr aw a F ev g ev Suppementa Figure 3. ffect of pateets tumor growth. () Pateet counts in bood of mice with 78 intraperitea ovarian cancer at the time of necropsy after twice weeky injecti with anti-pateet antibody (P) or pateet transfusis (n=3 mice per group). () Mean aggregate tumor weight in mice harboring intraperitea SKOV3ip1 ovarian tumors after twice weeky injecti with P or pateet transfusis (n=8 mice per group). () YFP-abeed pateets inside the tumor microenvirment after i.v. transfusi. Scae bar μm. Representative images of tumors from at east mice. () Rate of proiferati of Hey8 ces in vi after direct or indirect (with endotheia ce moayer transwe as separati) pateet exposure or Hey8 ces exposed to pateet reeasate (n=3). () Mean aggregate tumor weight of 78ip intraperitea tumors in c mice, mice treated with P (started day 1) or P pus e week of bevacizumab with subsequent (n= per group). (F) Mouse angiogenesis array of isoated pateet proteins from 78ip tumor bearing mice of indicated groups (n=). P <.1, P <.1, P <. (1-way NOV foowed by a Tukey s mutipe comparis post-hoc test in,,, ). veraged data are presented as the mean ±SM.
4 FK T Hey8 si si mfk si. MO si mfk 1 ka 38 ka % Proiferati 6 3 si 1 si mfk % poptosis 1 si si mfk mfk sirn, mfk sirn 1 mfk sirn, mfk sirn Suppementa Figure 4. sirn-mediated knockdown of FK. () fficiency of mouse-specific sirn tested in the human ovarian cancer ce ine Hey8 (1nM f.c.) and mouse ovarian endotheia ces (4nM and 1nM f.c.). eta-actin was used as oading c (n=). Proiferati () and apoptosis () of Hey8 ces 48 and 7 hours after transfecti of mouse-specific FK sirn (n=3). (, ) Mean aggregate tumor weight () and average number of nodues () of intraperitea Hey8 tumors after of bevacizumab treatment, twice weeky injecti of mouse-specific sirn and combinati of both (n=9-1 mice per group). P <.1, P <.1, P <. (two-taied Student s t-test in, ; 1-way NOV foowed by a Tukey s mutipe comparis post-hoc test in, ). veraged data are presented as the mean ±SM.
5 oxp Fak/Ptk oxp 1 1 Fak f/f; Pf4-cre+ Proiferati (re. to c) (x 1^6 /ml) Pateet counts g s s pt pt T /W akf Fak f/f; Pf4-cre+. (re. to c) 1. poptosis F W s s t pt p T -/K F F4/8+ g Fak f/f; Pf4-cre+ dr aw a o ng t w ith ro dr aw a o ng pts w ith GPH 3 FK re. FK expressi Fak-/- x Pf4 re promotor 4 GP1b-β 31 Fak f/f; Pf4-cre+ Suppementa Figure. Pateet-specific KO of FK. () Schema of pateet-specific FK KO mice using Pf4 as a tissue specific promotor. Vaidati of absence of FK expressi in pateet protein ysates. qrt-pr anaysis for FK expressi in 19+ -ces, 3+ T-ces and F4/8+ macrophages, isoated from the speen of and Fak f/f; Pf4-cre+ mice (n=). () verage number of nodues in and Fak f/f;pf4-cre+ mice after or g-term treatment with antibody (7-1 mice per group). () xtravasated pateets in and FK KO tumors. Representative images of tumors from at east mice per group. () Pateet counts in and KO mice in indicated groups (n=3). () Proiferati and (F) apoptosis rates of I8-VGF mouse ovarian cancer ces co-incubati with or FK-/- pts (n=3). P <.1, P <.1, P <. (two-taied Student s t-test in ; 1-way NOV foowed by a Tukey s mutipe comparis post-hoc test in, -F). veraged data are presented as the mean ±SM.
6 GSK698 GSK µm µm pfk pfk FK FK T T pfk/fk pfk/fk GSK698 (µm) GSK698 (µm) xtravasated pateets (per HPF). FKi 3 1 FKi Pazopanib ombinati FKi Pazopanib ombinati Suppementa Figure 6. ffect of the FK inhibitor GSK698 ovarian cancer growth. () Western bot anaysis and densitometry for p(y397) and tota FK in SKOV3ip1 ces treated with GSK698 (n=). β-actin was used as oading c. () Western bot anaysis and densitometry for p(y397) and tota FK in Hey8 ces treated with GSK698 (n=). β-actin was used as oading c. () Number of extravasated pateets in SKOV3ip1 c tumors and tumors treated with GSK698 (quantificati in tumors of at east mice per group). Mean aggregate tumor weight () and average number of tumor nodues () of Hey8 intraperitea tumors after treatment with GSK698 (FKi), pazopanib or a combinati of both drugs (n=-6 mice per group in, ). P <.1, P <.1, P <. (two-taied Student s t-test in ; 1-way NOV foowed by a Tukey s mutipe comparis post-hoc test in, ). veraged data are presented as the mean ±SM.
7 6 4 (Paz) 3 1 g Paz (Paz), FKi (ev) g ev (ev), FKi 1 1 short Paz g Paz short Paz, FKi short ev g ev short ev, FKi short Paz g Paz short Paz, FKi short ev g ev short ev, FKi Suppementa Figure 7. ffect of FK inhibiti of tumor outgrowth after of anti-angiogenic therapy. () verage number of nodues of orthotopic SKOV3ip1 tumors after anti-angiogenic therapy (Paz; pazopanib or ev; bevacizumab) ae or in combinati with the FK inhibitor GSK698 (FKi). Mean aggregate tumor weight () and average number of tumor nodues () of orthotopic Hey8 tumors after anti-angiogenic therapy (Paz; pazopanib or ev; bevacizumab) ae or in combinati with the FK inhibitor GSK698 (FKi) (n=6-9 mice per group in -). P <.1, P <.1, P <. (1-way NOV foowed by a Tukey s mutipe comparis post-hoc test in -). veraged data are presented as the mean ±SM.
Supplemental Figure 1. Isolation and characterization of CD133+ neurosphere-like
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SUPPLEMENTARY INFORMATION
DOI: 1.18/ncb8 In the format provided by the authors and unedited. a b c d 6 Li et a. Lee et a. Tung et a. e Mbn expressi intensity H C C Liver E1. E1. E1. E1. E16. E. E18. D ay D ay D ay7 D ay1 D ay1
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a Marker Ripk3 +/ 5 bp 3 bp b Ischemia (3 min) Reperfusion (4 h) d 2 mg/kg i.p. 1 w 5 w Sacrifice for IF size A subset for echocardiography and morphological analysis c Ischemia (3 min) Reperfusion (8
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p = formed with HCI-001 p = Relative # of blood vessels that formed with HCI-002 Control Bevacizumab + 17AAG Bevacizumab 17AAG
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Figure S1. PMVs from THP-1 cells expose phosphatidylserine and carry actin. A) Flow
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Supplementary Figure 1
Supplementary Figure 1 a γ-h2ax MDC1 RNF8 FK2 BRCA1 U2OS Cells sgrna-1 ** 60 sgrna 40 20 0 % positive Cells (>5 foci per cell) b ** 80 sgrna sgrna γ-h2ax MDC1 γ-h2ax RNF8 FK2 MDC1 BRCA1 RNF8 FK2 BRCA1
Supplementary Figure S1
Supplementry Figure S1 d MAP2 GFAP e MAP2 GFAP GFAP c f Clindin GFAP Supplementry Figure S1. Neuronl deth nd ltered strocytes in the rin of n ffected child. Neuron specific MAP2 ntiody stining in the hippocmpus
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Figure 1. CD163 -/- mice exhibit a similar phenotype ass WT mice in the absence of ischemic injury. a, Laser Doppler analysiss with perfusion quantitation at baseline (n= =10 per group). b, Immunostaining
Loss of RhoA promotes skin tumor formation. Supplementary Figure 1. Loss of RhoA does not impair F-actin organization.
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Supplementary Figure 1. Experimental paradigm. A combination of genome and exome sequencing coupled with array-comparative genome hybridization was
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Infect MCF-7 cells carrying dcas9-vp64 + psm2-p65-hsf1 with SAM library or vector. Introduce AKT reporter
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Supplemental Figure S1. RANK expression on human lung cancer cells.
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E (orthotopic implantation)
in vitro, (2D) in vitro, Matrigel on-top (MoT; 3D) non-metastatic metastatic D2 proliferation (% Ki67-positive) 59 65 66 apoptosis (% TUNEL-positive) 3 2 E (orthotopic implantation) D2 tumor incidence
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(control) or 16 h. The pretreated platelets were analyzed for mitochondrial membrane potential
SUPPLEMENTAL DATA Supplemental Figures Supplemental Figure 1 Supplemental Figure 1 Apoptotic events of stored platelets. (A and B) PRP was incubated at 37 under agitation for 0 (control) or 16 h. The pretreated
GFP/Iba1/GFAP. Brain. Liver. Kidney. Lung. Hoechst/Iba1/TLR9!
Supplementary information a +KA Relative expression d! Tlr9 5!! 5! NSC Neuron Astrocyte Microglia! 5! Tlr7!!!! NSC Neuron Astrocyte! GFP/Sβ/! Iba/Hoechst Microglia e Hoechst/Iba/TLR9! GFP/Iba/GFAP f Brain
Supplemental Figures:
Supplemental Figures: Figure 1: Intracellular distribution of VWF by electron microscopy in human endothelial cells. a) Immunogold labeling of LC3 demonstrating an LC3-positive autophagosome (white arrow)
(a-r) Whole mount X-gal staining on a developmental time-course of hearts from
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 Supplementary Figure 1 (a-r) Whole mount X-gal staining on a developmental time-course of hearts from Sema3d +/- ;Ephb4 LacZ/+ and Sema3d -/- ;Ephb4 LacZ/+ embryos.
Supplementary Figure 1: Hsp60 / IEC mice are embryonically lethal (A) Light microscopic pictures show mouse embryos at developmental stage E12.
Supplementary Figure 1: Hsp60 / IEC mice are embryonically lethal (A) Light microscopic pictures show mouse embryos at developmental stage E12.5 and E13.5 prepared from uteri of dams and subsequently genotyped.
Nature Immunology: doi: /ni eee Supplementary Figure 1
eee Supplementary Figure 1 Hyphae induce NET release, but yeast do not. (a) NET release by human peripheral neutrophils stimulated with a hgc1 yeast-locked C. albicans mutant (yeast) or pre-formed WT C.
Supplementary Information
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Figure S1, Beyer et al.
Figure S1, eyer et al. Pax7 Myogenin si sitrl Hoechst T = 72h 14 1.8.6.4.2 12 1 8 6 4 2 24h 48h 96h diff. sitrl siset1 212 72h diff. b1 td r t Se km MyH Vinculin Myogenin β-ctin Vinculin MW b1 ka td r
Atg5 flox/flox ; CAG-Cre, 19M brain heart lung. spleen stomach colon. Takamura_Fig. S1
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To determine the effect of over-expression and/or ligand activation of. PPAR / on cell cycle, cell lines were cultured as described above until ~80%
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J. Cell Sci. 129: doi: /jcs : Supplementary information
Movie 1. AgLDL is contained in small sub-regions of the lysosomal synapse that are acidic. J774 cells were incubated with agldl dual labeled with a ph sensitive and a ph insensitive fluorophore for 1 hr.
Supplementary Information
Supplementary Information Figure S1: Follicular melanocytes in the wound peripheral area migrate to the epidermis in response to wounding stimuli. Dorsal skin of Trp2-LacZ mice stained with X-gal and analyzed