OIE Reference Laboratory Reports Activities

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1 OIE Reference Laboratory Report Activitie Activitie in 2017 Thi report ha been ubmitted : :02:17 Name of dieae (or topic) for which you are a deignated OIE Reference Laboratory: n wine fever Addre of laboratory: Ah Road, Pirbright Woking, Surrey, GU24 0NF UNITED KINGDOM Tel.: Fax: addre: linda.dixon@pirbright.ac.uk Webite: Name (including Title) of Head of Laboratory (Reponible Official): Dr Bryan Charlton Name (including Title Poition) of OIE Reference Expert: Dr Linda Dixon Which of the following define your laboratory? Check all that apply: Reearch OIE Reference Laboratory Report Activitie,

2 ToR 1: To ue, promote dieminate diagnotic method validated according to OIE Stard 1. Did your laboratory perform diagnotic tet for the pecified dieae/topic for purpoe uch a dieae diagnoi, creening of animal for export, urveillance, etc.? (t for quality control, proficiency teting or taff training) Diagnotic Tet Indicated in OIE Manual (/) Total number of tet performed lat year Indirect diagnotic tet Nationally Internationally ELISA YES 0 0 Direct diagnotic tet Nationally Internationally Real-time PCR YES 10 0 ToR 2: To develop reference material in accordance with OIE requirement, implement promote the application of OIE Stard. To tore ditribute to national laboratorie biological reference product any other reagent ued in the diagnoi control of the deignated pathogen or dieae. 2. Did your laboratory produce or upply imported tard reference reagent officially recognied by the OIE? 3. Did your laboratory upply tard reference reagent (non OIE-approved) /or other diagnotic reagent to OIE Member Countrie? 2 OIE Reference Laboratory Report Activitie, 2017

3 Type of reagent available Related diagnotic tet Produced/ provide Amount upplied nationally (ml, mg) Amount upplied internationally (ml, mg). of recipient OIE Member Countrie Region of recipient ASFV OURT88/3 PCR Provide 0 2ml 1 RNA from ASFV infected cell PCR Provide 16 x 1ml 0 1 ASFV Georgia (DP148R) ASFV Georgia (I329L) iolate viru iolation Provide 0 6ml 1 moue monoclonal antibodie againt ASFV protein p30 p72 cell taining Provide 0 2ml 1 fixed tiue from immunied nonimmunied pig infected with ASFV Georgia 2007/1 hitopathology Provide 56g Did your laboratory produce vaccine? OIE Reference Laboratory Report Activitie,

4 5. Did your laboratory upply vaccine to OIE Member Countrie? ToR 3: To develop, tardie validate, according to OIE Stard, new procedure for diagnoi control of the deignated pathogen or dieae 6. Did your laboratory develop new diagnotic method validated according to OIE Stard for the deignated pathogen or dieae? 7. Did your laboratory develop new vaccine according to OIE Stard for the deignated pathogen or dieae? ToR 4: To provide diagnotic teting facilitie,, where appropriate, cientific technical advice on dieae control meaure to OIE Member Countrie 8. Did your laboratory carry out diagnotic teting for other OIE Member Countrie? 9. Did your laboratory provide expert advice in technical conultancie on the requet of an OIE Member Country? ToR 5: To carry out /or coordinate cientific technical tudie in collaboration with other laboratorie, centre or organiation 10. Did your laboratory participate in international cientific tudie in collaboration with OIE Member Countrie other than the own? 4 OIE Reference Laboratory Report Activitie, 2017

5 Title of the tudy Duration Purpoe of the tudy Partner (Intitution) OIE Member Countrie involved other than your country Annual report IAEA Reearch Contract: CRP32032 Veterinary Diagnotic Laboratory Network (VETLAB Network) to prevent control tranboundary animal dieae (TAD) 5 year total Develop validated reagent, build capacity for diagnoi ANSES, CIRAD, IAEA, CSIRO,SENASA, Pirbright, LANAVET,CVI, LANADA, UKIM,ONSSA, NAHDIC ARGENTINA AUSTRALIA AUSTRIA CAMEROON COTE D'IVOIRE CROATIA ETHIOPIA FRANCE MACEDONIA (FORMER YUG. REP. OF) MOROCCO SUDAN ToR 6: To collect, proce, analye, publih dieminate epizootiological data relevant to the deignated pathogen or dieae 11. Did your Laboratory collect epizootiological data relevant to international dieae control? 12. Did your laboratory dieminate epizootiological data that had been proceed analyed? 13. What method of diemination of information i mot often ued by your laboratory? (Indicate in the appropriate box the number by category) a) Article publihed in peer-reviewed journal: 5 1. Inferring within-herd tranmiion parameter for n wine fever viru uing mortality data from outbreak in the Ruian Federation. 1. Guinat C, Porphyre T, Gogin A, Dixon L, Pfeiffer DU, Gubbin S. Tranbound Emerg Di v 9. doi: /tbed [Epub ahead of print] PMID: Aria M, de la Torre A, Dixon L, Gallardo C, Jori F, Laddomada A, Martin C, Parkhoue RM, Revilla Y, Rodriguez FAJ; Sanchez-Vizcaino. Vaccine (Bael) Oct 7;5(4). pii: E35. doi: /vaccine Review. PMID: [PubMed] Approache Perpective for Development of n Swine Fever Viru Vaccine. 3. Guinat C, Vergne T, Jurado-Diaz C, Sánchez-Vizcaíno JM, Dixon L, Pfeiffer DU. Vet Rec Jan 28;180(4):97. doi: /vr Epub 2016 v 15. Review. PMID: [PubMed - indexed for MEDLINE] Free PMC Article Effectivene practicality of control trategie for n wine fever: what do we really know? 4. Guinat C, Gogin A, Blome S, Keil G, Pollin R, Pfeiffer DU, Dixon L. Vet Rec Mar 12;178(11): doi: /vr Review. PMID: Tranmiion route of n wine fever viru to dometic pig: current knowledge future reearch OIE Reference Laboratory Report Activitie,

6 direction. 5. Davie K, Goatley LC, Guinat C, Netherton CL, Gubbin S, Dixon LK, Rei AL. Tranbound Emerg Di Apr;64(2): doi: /tbed Epub 2015 Jun 24. PMID: Survival of n Swine Fever Viru in Excretion from Pig Experimentally Infected with the Georgia 2007/1 Iolate. b) International conference: 0 c) National conference: 0 d) Other: (Provide webite addre or link to appropriate information) 0 ToR 7: To provide cientific technical training for peronnel from OIE Member Countrie To recommend the precribed alternative tet or vaccine a OIE Stard 14. Did your laboratory provide cientific technical training to laboratory peronnel from other OIE Member Countrie? a) Technical viit: 0 b) Seminar: 3 c) H-on training coure: 2 d) Internhip (>1 month): 0 Type of technical training provided (a, b, c or d) Country of origin of the expert() provided with training. participant from the correponding country c Japan 1 c Finl 1 b China 3 ToR 8: To maintain a ytem of quality aurance, bioafety bioecurity relevant for the pathogen the dieae concerned 15. Doe your laboratory have a Quality Management Sytem certified according to an International Stard? 6 OIE Reference Laboratory Report Activitie, 2017

7 Quality management ytem adopted UKAS Certificate can (PDF, JPG, PNG format) UKAS Scope.pdf 16. I your laboratory accredited by an international accreditation body? Tet for which your laboratory i accredited Real time PCR x 2 Ag ELISA Ab ELISA Accreditation body UKAS UKAS UKAS 17. Doe your laboratory maintain a biorik management ytem for the pathogen the dieae concerned? (See Manual of Diagnotic Tet Vaccine for Terretrial Animal, Chapter 1.1.4) ToR 9: To organie participate in cientific meeting on behalf of the OIE 18. Did your laboratory organie cientific meeting on behalf of the OIE? 19. Did your laboratory participate in cientific meeting on behalf of the OIE? ToR 10: To etablih maintain a network with other OIE Reference Laboratorie deignated for the ame pathogen or dieae organie regular inter-laboratory proficiency teting to enure comparability of reult 20. Did your laboratory exchange information with other OIE Reference Laboratorie deignated for the ame pathogen or dieae? 21. Wa your laboratory involved in maintaining a network with OIE Reference Laboratorie deignated for the ame pathogen or dieae by organiing or participating in proficiency tet? OIE Reference Laboratory Report Activitie,

8 Purpoe of the proficiency tet: 1 Role of your Reference Laboratory (organier/ participant). participant Participating OIE Ref. Lab/ organiing OIE Ref. Lab. Detection of genome antibodie Participant? Pirbright, UK, Spain 1 validation of a diagnotic protocol: pecify the tet; quality control of vaccine: pecify the vaccine type, etc. 22. Did your laboratory collaborate with other OIE Reference Laboratorie for the ame dieae on cientific reearch project for the diagnoi or control of the pathogen of interet? ToR 11: To organie inter-laboratory proficiency teting with laboratorie other than OIE Reference Laboratorie for the ame pathogen dieae to enure equivalence of reult 23. Did your laboratory organie or participate in inter-laboratory proficiency tet with laboratorie other than OIE Reference Laboratorie for the ame dieae? te: See Interlaboratory tet comparion in: Laboratory Proficiency Teting at: ee point 1.3 Purpoe for inter-laboratory tet comparion 1. participating laboratorie Region() of participating OIE Member Countrie Detection of genome antibodie >28 ToR 12: To place expert conultant at the dipoal of the OIE 24. Did your laboratory place expert conultant at the dipoal of the OIE? 25. Additional comment regarding your report: 8 OIE Reference Laboratory Report Activitie, 2017

9 OIE Reference Laboratory Report Activitie,

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