Effect of adding linseed oil on in vitro gas and methane production and some fermentation characteristics

Transcription

1 EUROPEAN ACADEMIC RESEARCH Vol. VI, Issue 1/ April 2018 ISSN Impct Fctor: (UIF) DRJI Vlue: 5.9 (B+) Effect of dding linseed oil on in vitro gs nd methne production nd some fermenttion chrcteristics A. H. KUTTAR Ministry of Science nd Technology Directorte of Agriculturl Reserch MAJID HAMID RASHID College of Agriculture, University of Diyl Animl Production Deprtment ASHWAQ ABD ALI College of Agriculture, University of Bghdd Animl Production Deprtment Astrct: This experiment ws crried out in niml feeding lortories in the Animl Production Deprtment of the Fculty of Agriculture, University of Bghdd / Jdriy for the period from 1/12/2016 to 1/5/2017, where food supplements were used with different percentges of Linseed oil. 0,70,140,280 μl / kg dry mtter to mixture of concentrted feed nd corse feed to study its effect on totl gs production nd methne gs nd fermenttion properties ((ph, voltile ftty cid rtios (Acetic, propionic nd utyric), mmoni nitrogen rtio)). Results: 1-. Using the gs production technology in the lortory, significnt reduction in the totl production of gs nd methne in T2, T3, contining 70,140 μl / kg dry mteril from LO oil t incution time fter 12 hours in vitro compred to T1nd T4 (supplemented with 280 μl / kg dry mtter of LO oil). After 24 hours of in vitro incution, T2 tretment ws significntly reduced (p 0.01) in totl gs production compred to T3 nd T4, For the production of methne, it ws significntly lower (p 0.01) for T2 compred to T3 nd T4 respectively. The totl volume of gs fter 48 hours of in vitro incution ws significntly decresed (p 0.01) for T2, T3 nd T4 compred to the control tretment, the sme T6 nd T7 were significntly lower (p 0.01) in methne compred to T2 nd fter 72 hours of lortory incution, T2 nd T4 showed significnt decrese (p 0.01) in totl gs production while T6 ws centrl etween them nd there were no differences In the size of methne in the models withdrwn fter 72 hours of in vitro incution. 2- For fermenttion chrcteristics, the results showed the following: 59

2 A. H. Kuttr, Mjid Hmid Rshid, Ashwq Ad Ali- Effect of dding linseed oil on in vitro gs nd methne production nd some fermenttion chrcteristics 2-1 There were significnt differences (P 0.01) in the ph vlues of the withdrwn smples fter 12 hours of in vitro incution compred to the control tretment, the T4 ws significntly reduced (P 0.01) in the ph vlues in the withdrwn models fter 24 nd fter 48 nd 72 hours of lortory incution. The T4 ws significntly lower (P 0.01) in the ph vlue compred to T2 nd T3 respectively. 2-2 As for the concentrtion of mmoni nitrogen, The results showed tht there were significnt differences (P 0.01) etween the LO oil dded to the hy in the concentrtion of mmoni nitrogen where T2, T3 nd T4 contining LO were significntly reduced (P 0.01) in nitrogen concentrtion Ammoni compred to T1 tretment of the withdrwn smples fter 12 nd 24 hours of lortory incution. The results lso showed significnt decrese in (p<0.01) in the mmoni nitrogen concentrtion of T3 nd T4 for the withdrwn smples fter 48 nd 72 hours of lortory incution compred to T2 tretment. 2-3 The results showed tht there were significnt differences (p<0.01) etween the LO oil of the hy in the concentrtion of voltile ftty cids (cetic cid, utyric cid, propionic), where T2 nd T3 were significntly lower (p<0.01) 12 dys of incution in vitro compred to T4 tretment, while no significnt differences in cid concentrtion were oserved 24 hours of lortory incution. T2 ws significntly lower (p<0.01) in cetic cid concentrtion compred to T3 nd T4 respectively, There were significnt differences(p<0.01) in cid concentrtion In the smples withdrwn fter 72 hours of in vitro incution. As for propionic cid, LO (T5) showed significnt reduction(p<0.01) in the concentrtion of propionic cid fter 12 in vitro incution compred with T1 while T3 nd T4 were significnt(p<0.01) The concentrtion of propionic cid fter 24 nd 48 hours of in vitro incution, while the concentrtion of cid nd high morle (p<0.01) in the tretment T2 compred with the tretment T4, nd the results showed no significnt differences of LO oil tretments in the concentrtion of utyric cid hs 12 nd 24 hours incution in the lortory, Results showed high morle (p<0.01) concentrtion Butyric cid for tretment T2 compred to control tretment fter 48 nd 72 hours of incution lortory. Key words: linseed oil, in vitro gs nd methne production, fermenttion chrcteristics INTRODUCTION Animl production hs incresed the use of synthetic chemicls s feed dditives for niml feed (Clsmigli et l., 2006), which improves growth nd microil metolic processes (Fellner, 2002) nd improves milk mngement for ruminnts (cttle, sheep, Gots), ut recently there hve een concerns out the use of these synthetic chemicls s food supplements in niml feed ecuse of potentil toxic hzrds resulting from the use of these Artificil chemicls, Which incresed the 60

3 A. H. Kuttr, Mjid Hmid Rshid, Ashwq Ad Ali- Effect of dding linseed oil on in vitro gs nd methne production nd some fermenttion chrcteristics prediction of the risks of these chemicls to the environment nd helth ssocited with the use of these synthetic chemicls. All these ostcles mde the governmentl institutions nd regultory odies, including the Europen Union in 2006, to consider limiting the use of some synthetic nd chemicl mterils in niml feed. Chemicls re ntiiotics (Brton, 2000), prompting reserchers nd niml production professionls to find food lterntives of plnt origin tht re derived minly from vegetle sources, such s vegetle oils (Cown, 1999), They re usully extrcted from plnt mterils y oiling wter nd distilltion of stem, ut other methods include the use of solvents nd the use of supercriticl cron dioxide. The expression of vegetle oils (Benchr nd Grethed, 2011) hs een used to e sfe lterntive to ntiiotics in Ruminnts, where there re numer of types of vegetle oils nd these oils Flxseed oil (Usittissimum) or flxseed oil is the oil extrcted from the flxseed through the times followed y extrction with orgnic solvents, is colorless liquid to yellowish nd rich in unsturted ftty cids Aric for ody nd knowledge Populrly clled the hot oil or Also with drying oil due to the possiility of conversion into solid form y polymeriztion. Flxseed oil cn e used lone or mixed with mixture of other oils, resins or solvents s suppressed seed (Thompson, 2003). linseed oil is used s dietry supplement in the diet ecuse it is mjor source of α-phenylenolic cid, (Vereshgin nd Novitsky, 1965) (etween 42-48% ALA "C18: 3") (Diller nd Diller, 2004), This cid is reduced in the ody to limited mount of Eicospentenoic cid (EPA) or known omeg-3 is the sme cid found in fish oil, which in turn turns into three series of prostglndins nd is semi-hormones tht re mnufctured in mny prts of the ody. The purpose of this study ws to dd linseed oil with different percentges to the ruminnts' diet consisting of 80% lflf hy with 20% of concentrted feed nd to study its effect on the totl production of gs nd methne in the lortory nd mesure some 61

4 A. H. Kuttr, Mjid Hmid Rshid, Ashwq Ad Ali- Effect of dding linseed oil on in vitro gs nd methne production nd some fermenttion chrcteristics fermenttion properties such s voltile ftty cids, mmoni nitrogen, ph After different incution lortory periods. MATERIAL AND METHODS 1- Experiment Pln: The experiment ws crried out in the nutrition lortories of the Fculty of Agriculture / University of Bghdd-Jdriy, to study the effect of dding different levels of flxseed oil y nd 280 μl / kg to mixture of concentrted feed nd corse feed. The effect of feed type nd ddition of cstor oil on the pttern of rumen fermenttion, totl gs production nd methne gs in the lortory. 2- Experiment Diet: The components of the experimentl ush used in the study were provided from the niml field of the University of Bghdd / Fculty of Agriculture / Animl Resources Deprtment consisting of 80% of lflf hy + concentrted feed 20% with the ddition of linseed oil (140,70 nd 280 micro liters / kg DM). In this study, the use of lflf hy should e crushed with lortory jr nd dimeter of 1 mm in order to fcilitte mixture with concentrted feed nd oil with completely efore strting the experiment Tle 1: Percentge nd components of rw mterils included in the composition of concentrted nutritionl diets used in the experiment. Rw Mterils % Brley 23 Whet rn 22 Soyen Mel 13 yellow corn 40 Minerls nd vitmins 1 Slt 1 Crud protein* ME(MJ/Kg DM) *Clculted from the chemicl nlysis tle for Irqi feed mterils (Al- Khwj, 1978) (on DM% sis) ME(MJ/Kg DM)=0.012 CP EE CF NFE (MAFF, 1975) 62

5 A. H. Kuttr, Mjid Hmid Rshid, Ashwq Ad Ali- Effect of dding linseed oil on in vitro gs nd methne production nd some fermenttion chrcteristics 3-Chemicl composition of primry feed mterils nd experimentl diets Tle ( 2 nd 3) shows the chemicl composition of the concentrtes of the concentrtes nd the experimentl grits with the dded LO oil. The dry mtter(dm), orgnic mtter(om), crud protein(cp), crud fier(cf), Ether Extrct(EE) nd nitrogen-free extrct(nfe) were estimted y AOAC (1984) In the nutrition lortory of the Fculty of Agriculture - University of Bghdd / Deprtment of Animl Production. As for the metolizle energy, hs een clculted under the following eqution: ME(MJ/Kg DM)=0.012XCP+0.031XEE+0.005XCF+0.014XNFE According to in vitro digestiility of DM* nd OM y method (Tilley nd Terry,1963). Tle 2: shows the chemicl composition of concentrted nd roughge feed in the composition of the nutritionl diets used in the experiment %. fctor Ingredient Brley yellow corn Soyen Mel Whet rn lflf hy DM % of fresh OM% Ash% CP% CF% EE% *ME(MJ/Kg DM)=0.012XCP+0.031XEE+0.005XCF+0.014XNFE NFE(%OFDM)=%OM-(%CP+%CF+%EE) NFE % ME*(MJ/Kg DM) Tle 3: : shows Chemicl composition nd metolizle energy (MJ / kg DM) for 80 % lflf hy with 20% concentrted feed nd dding Linseed oil t 0.70,140 nd 280 μl* / kg DM fctor Tretments DM % of fresh OM% Ash% CP% CF% EE% NFE % ME(MJ/Kg DM) T1(control) T2 dd 70 μl LO T3 dd 140 μl LO T4 dd 280 μl LO T1=control (without ny ddition) T2= treted dding Linseed oil t 70 μl/ kg DM

6 A. H. Kuttr, Mjid Hmid Rshid, Ashwq Ad Ali- Effect of dding linseed oil on in vitro gs nd methne production nd some fermenttion chrcteristics T3= treted dding Linseed oil t 140 μl/ kg DM T4= treted dding Linseed oil t 140 μl/ kg DM * μl=micro liters ME(MJ/Kg DM)=0.012X CP+0.031XEE+0.005XCF+0.014XNFE (MAFF, 1975) RESULTS AND DISCUSSION 1-Production of totl gs nd methne gs in the lortory The results of Tle 4 showed significnt decrese in (p<0.01) in totl gs production fter 12 hours of lortory incution when LO oil ws dded, Tht lowest quntity ws ml / 200 mg dry mtter in T2 followed y T4 (33.25 ml / 200 mg DM) compred with T ml / 200 mg dry mtter. The results showed significnt decrese in the production of methne gs for T2 nd T3 (3.43 nd 3.40 mg / 200 mg dry mtter respectively) compred with T4 (4. 50 ml / 200 mg dry mtter), In the sme s tht significnt decrese (P <0.01) in totl gs production when LO oil ws dded fter 24 hours, As T3 nd T4 were recording the highest mount (P <0.01) nd ml / 200 mg dry mtter respectively) compred with T2 tretment (36.25 ml / 200 mg dry mtter). As for the mount of methne fter 24 hours of lortory incution, Tle 4 reveled the existence of significnt differences etween the trnsctions in the volume of methne dded y the LO oil. The trnsction T2 recorded high morle (p<0.01) with n verge of 4.50 ml / 200 Mg dry mtter) in methne production compred to T3 nd T4 (5.00 nd 5.20 ml / 200 mg dry mtter, respectively) The results of Tle 4 lso showed tht the highest significnt volume (p <0.01) of totl gs when dding LO oil fter 48 hours of lortory incution ws in T1 (45.25 ml / 200 mg dry mtter) compred with tretment in T2 (36.25 ml / 200 mg) followed(t3 nd T nd ml / 200 mg dry mtter respectively). For the LO oil tretments, the results of Tle 4 showed significnt decrese (p <0.01) for T3 nd T4 (5.00 nd 5.00 ml / 200 mg dry mtter) compred to T2 (

7 A. H. Kuttr, Mjid Hmid Rshid, Ashwq Ad Ali- Effect of dding linseed oil on in vitro gs nd methne production nd some fermenttion chrcteristics mg / 200 mg dry mtter). These results re consistent with those of Mrino et l. (2013), At the sme time These results do not gree with Moreir et l. (2014), which indicted tht dding different levels of vegetle oils y 0, 30, 50 nd 70 resulted in significnt reduction in methne production, The reson for the decrese in the production of gs when dding oil to the crops my e due to the modifiction of the fermenttion process nd the decrese in its effectiveness due to the presence of cover of oil on the feed mteril, which is considered s sic food for rumen cteri (Mnso et l., 2009) For the feed mteril or perhps the dded oil contins some toxic sustnces tht re hrmful to the Microorgnisms, especilly cellulose nd cellulose fiers in the rumen. Tle 4. Effect of linseed oil supplementtion y 0, 70, 140 nd 280 μl / kg DM on totl gs production nd methne (200 mg / DM) for the 80% lflf hy with 20% concentrted feed t different lortory incution periods. Studied Trit Totl gs Volume Methne Volume Totl gs Volume Std ± men Methne Volume Totl gs Volume Methne Volume Totl gs Volume Methne Volume Tretments Incution periods(hour) T1(control) T2 dd 70 μl LO T3 dd 140 μl LO 36.70± ±1.25 c 33.25± ± ± ± ± ± ± ± ± ± ± ±0.62 d 39.75±0.47 c 5.05± ± ± ± ±0.64 c 43.00±1.08 c 5.15± ± ±0.64 T4 dd 280 μl LO Significnt 35.45± ± ± ± ± ± ±0.64 T1: control(without ddition), T2: Diet dding linseed oil y 70 micro liters / kg DM, T3: Diet Adding linseed oil y 140 micro liters / kg DM, T4: Diet Adding linseed oil y 280 micro liters / kg DM, N.S indictes not significnt, indictes significnt differences t the proility level(p <0.01). DM=dry mtter LO=linseed oil 4.90±0.75 N.S The results lso show tht when LO oil ws dded to the diet, significnt reduction (P<0.01)ws chieved in the mount of totl gs produced fter 72 hours of in vitro incution for T2 65

8 A. H. Kuttr, Mjid Hmid Rshid, Ashwq Ad Ali- Effect of dding linseed oil on in vitro gs nd methne production nd some fermenttion chrcteristics tretment (41.50 ml / 200 mg dry mtter) compred to T4 (45.50 ml / 200 mg dry mtter),these results do not gree with Morgvi et l.(2012),t the sme time The results lso showed no significnt differences in methne production fter 72 hours of in vitro incution in the ddition of LO oil, which is consistent with Murply et l. (1990) nd inconsistent with Mizuuti et l. (2015). ) This my e due to the reflection of the effectiveness nd extent of the degrdtion of food nd feed digestiility which contining high percentge of lignin tht ffect the effectiveness of the microiology of nlyzed fier nd thus ffect the mount of gs produced in the rumen. 2- PH mesurement The results of the sttisticl nlysis t the ph mesurement shown in Tle (5) show the effect of the ddition of LO oil y 0, 70, 140 nd 280 μl / kg dry mtter of the 80% lflf hy with 20% of concentrted feed in incution periods 12,24,48 nd 72 hours in vitro, there T3 nd T4 were significntly higher in (P <0.01) fter 12 hours of lortory incution (6.65,6.60) thn T1 t (6.35) while T2 ws intermedite etween them. Also The results indicted tht T4 recorded the lowest ph vlue(6.60) in the models (P<0.01) compred with T2 nd T3 (7.05 nd 7.10) compred T1(6.55), fter 24 hours in vitro incution. while the ph of T4 nd T1 were significntly reduced (P<0.01) (6.75,6.70) compred with T2 nd T3 (7.10 nd 7.20),As fter 48 hours in vitro incution, As for the smples with tken fter 72 hours of lortory incution, the T4 ws significntly lower (P<0.01) t (6.75) compred to T2 nd T3 contining LO oil (7.00 nd 6.95 respectively). These results were similr to those of Shingfield (2003) nd similr to (Musiu et l., 2015) ecuse their reserch ws sed on the ddition of different percentges of lipid supplementtion with one of the ntiiotics(monensein) to the roughges feed, The results were similr with those of Jlc et l. (2007) when two types of vegetle oils (LO oil nd 66

9 A. H. Kuttr, Mjid Hmid Rshid, Ashwq Ad Ali- Effect of dding linseed oil on in vitro gs nd methne production nd some fermenttion chrcteristics soyen oil) were dded, nd they were not similr (Sterk et l., 2011), which indicted tht the concentrtion of ph decresed with the ddition of vegetle oil This my e due to the survivl of microorgnisms in Crsh with incresed estrogen degrdtion nd incresed concentrtion of volt ftty cids t time dded y the oil to the diets. Tle 5. The effect of dding linseed oil y 0, 70, 140 nd 280 μl / kg dry mtter, on the ph of the lflf hy (80%) with the concentrted feed (20%) in different incution periods. Studied Trit 12 Std ± men 24 PH Incution periods(hour) Tretments T1(control) 0.05 ± ± ± ±0.05 T2 dd 70 μl LO 6.45± ± ± ±0.005 T3 dd 140 μl LO 6.65± ± ± ±0.15 T4 dd 280 μl LO 6.60± ± ± ±0.005 Significnt T1: control(without ddition), T2: Diet dding linseed oil y 70 micro liters / kg DM, T3: Diet Adding linseed oil y 140.micro liters / kg DM, T4: Diet Adding linseed oil y 280 micro liters / kg DM, indictes.significnt differences t the proility level(p <0.01). 3- Mesuring the concentrtion of Nitrogen mmoni The results of Tle 6 show the concentrtion of mmoni nitrogen concentrtion in the rumen liquid for 80% lflf hy with 20% of concentrted feed t the effect of dding LO oil of 0, 70, 140 nd 280 μl / kg dry mtter in incution periods fter 12,24,48 nd 72 h in vitro, The results showed significnt differences (P<0.01) etween the concentrtions of nitrogen mmoni in the smples with tken fter 12 hours in lortory incution, tht T3 nd T4 showed significnt decrese in mmoni nitrogen concentrtion (P<0.01) t (31.05 nd mg / 100), compred with T1 nd T2(32.85 nd mg / 100 ml).also The results showed significnt reduction (P<0.01) for T2, T3 nd T4, which contined LO oil of (31.65,31.85 nd respectively), compred with the tretment of T1 (32.85 mg / 100 ml), After 24 hours of 67

10 A. H. Kuttr, Mjid Hmid Rshid, Ashwq Ad Ali- Effect of dding linseed oil on in vitro gs nd methne production nd some fermenttion chrcteristics lortory incution, These results were consistent with the findings of Szumcher et l. (2004) when dding different levels of vegetle oils to ruminnts' ruminnts, lso similr to those of Gunl et l. (2014) when dding vegetle oils t different levels 125, 250 nd 500 μl / kg dry mtter to clves Which resulted in reduced nitrogen concentrtion of mmoni in the diet, while T2 ws highest of mmoni nitrogen concentrtion(p<0.01) t (31.35 mg / 100 ml) compred with the tretments of T1,T3 nd T4(30.00, nd respectively) In smple tken fter 48 hours of in vitro incution. fter 72 hours of in vitro incution, The results showed tht tretment T3 nd T4 were significntly lower (P<0.01) t (30.55 nd mg / 100 ml ) thn T2 (31.25 mg / 100 ml), which ws supported y Mertens (2002). However, these results were not consistent with Vrgs et l. (2011) when dding LO oil y 50 g / Kg dry mtter which were reduced to the concentrtion of mmoni nitrogen to the rumen fluid, nd in the sme context Dore et l. (1994), In ddition vegetle oils to feeds tht reduces the nitrogen mmoni of concentrtion to the rumen, numer of reserchers, such s Wllce (2002), hve suggested tht the presence of vegetle oils s supplements or dietry supplements tht contin different levels of ftty cids, which cts s inhiitory gent for the reduce ctive of mmoni-producing cteri nd hrmful cteri In ddition to it reduces the effectiveness of the process y removing the mino group of proteins nd this cycle reduces the rte of production of nitrogen mmoni, therey incresing the use of protein microil. 68

11 A. H. Kuttr, Mjid Hmid Rshid, Ashwq Ad Ali- Effect of dding linseed oil on in vitro gs nd methne production nd some fermenttion chrcteristics Tle 6. Effect of the ddition of linseed oil y 0, 70, 140 nd 280 μl / kg dry mtter on nitrogen mmoni for 80% lflf hy with 20% concentrted feed in different lortory incution periods. Studied Trit 12 Std ± men Nitrogen mmoni Incution periods(hour) Tretments T1(control) T2 dd 70 μl LO T3 dd 140 μl LO T4 dd 280 μl LO Significnt 32.85± ± ± ±0.05c 32.25± ± ± ±0.005c 30.00± ± ± ± ± ± ± ±0.05c 4. Mesuring the concentrtion of voltile ftty cids (VFA) The results of Tle 7 show the concentrtion of voltile ftty cids (cetic, propionic, utyric cids) in the rumen liquid of the 80% lflf hy with 20% concentrted feed when dded LO oil y 0, 70, 140 nd 280 micro liters / kg dry mteril in vitro periods fter 12,24, 48 nd 72 h, The tretment T4 (62.90 mmol / L) ws significntly higher (P<0.01) in concentrtion of cetic cid on T2 nd T3 (62.13 nd mmol / L) In the smples tken from the rumen fluid fter 12 hours of lortory incution, While there ws no significnt difference in the concentrtion of cetic cid etween the tretment which were contining LO oil After 24 hours of lortory incution. After 48 hours of lortory incution, the concentrtion cetic cid ws significntly decresed(p<0.01) in the T2 which ws contining the (70 μl) LO oil t (65.26 mmol / L) compred with T3 nd T4 (66.30 nd 66.36) mmol / L respectively). The results showed significnt differences etween LO oil y 0.70, 140 nd 280 μl / kg dry mtter fter 72 hours of lortory incution, tht concentrtion cetic cid ws significntly decresed(p<0.01) in the T1,T2 nd T3 t (65.30,65.00 nd mmol / L) compred with T4(66.46 mmol/l), These results were consistent with Torl et l. (2009),As These results re not consistent with Atkinson et l. (2006), which dded tht the vegetle oil to the diet hd 69

12 A. H. Kuttr, Mjid Hmid Rshid, Ashwq Ad Ali- Effect of dding linseed oil on in vitro gs nd methne production nd some fermenttion chrcteristics positive reltionship to increse the concentrtion of cid nd increse the incution period up to 96 hours in the lortory. The Results (Tle 7) showed significnt differences (P<0.01) etween the tretment in the concentrtion of propionic cid in the smples tken fter 12 hours. A significnt decrese ws oserved (P<0.01) in T2,T3 nd T4 which were 26.36,2640 nd mmol / L Contining 70,140 nd 280 μl / kg dry mtter of LO oil, compred with T1(27.82 mmol / L). These results were similr to Oliveir et l. (2013), At the sme time These results re not consistent with Mrtin et l. (2010), which ws shown when dding glycerol to the diets nd three types, including glycerol digested nd pure glycerol nd the mixture etween the two types nd 15% of ech type of glycerol with ddition vegetle oil to the reltionship hs incresed the concentrtion of propionic cid fter 24 hours of lortory incution, While the results lso showed no significnt differences in the concentrtion of propionic cid etween the LO oil tretment fter 24 nd 48 hours of lortory incution, These results re not consistent with Getchew et l.(2004),tht were using in our experiment with ddition vegetle oil the mixture with diet contining 50% lflf hy, 18% of ll strw, corn stlks, nd 6.9% of soyen flour y incresing propionic cid in lflf hy. The results showed high significntly (P<0.01) in T4 (30.93 mmol / L) when LO oil ws dded fter 72 hours of lortory incution compred to T2 ndt3 (29.66 nd mmol / L) while T1 ws significnt decrese (P<0.01). These results re consistent Benchr et l. (2006). At the sme time the results re not consistent Dohme et l. (2011), Perhps it ws ecuse which dded tht lipids, hve significntly ffected the rtios of voltile ftty cids s they reduced the concentrtion of propionic cid in rumen fluid. The results (Tle 7) showed significnt effect from the ddition of LO oil to the lflf hy on the concentrtion of utyric cid fter 12, wheres significnt decrese ws oserved (P<0.01) in T2,T3 nd T4 which were (9.26,9.33 nd 9.36 mmol / 70

13 A. H. Kuttr, Mjid Hmid Rshid, Ashwq Ad Ali- Effect of dding linseed oil on in vitro gs nd methne production nd some fermenttion chrcteristics L) Contining 70,140 nd 280 μl / kg dry mtter of LO oil, compred with T1(10.08 mmol / L). while The lso results showed no significnt fter 24 hours of lortory incution, While the results showed tht were significnt differences (P<0.01) in the concentrtion of utyric cid in the smples tken fter 48 nd 72 hours. A significnt decrese ws oserved (P<0.01) in the concentrtion of utyric cid, decresed in tretment T2,T3 nd T4 significntly in the concentrtion of utyric cid for oth periods t (11.33,11.40,1156 nd 11.46,11.80 mmol / L) except T4 from period 72 h tht ws Recorded higher concentrtion of utyric cid(12.00 mmol / L) compred with control tretment T1 (11.86 nd mmol / L), These results were Similr to those otined y Doreu nd Chillird (1997), ut re not consistent with Aghjnzdeh-Golshni et l. (2015), which they mentioned tht the ddition of vegetle oils ws significnt in rumen fermenttion with microorgnism survivl nd high voltile ftty cid content nd precipittion, As well These results re conformity to those otined y Kristensen nd Hrmon (2004), wheres The ddition of vegetle oils hs improved the concentrtion of utyric cid in the mixture y keeping the ctivity of microorgnisms in rumen with incresing the decomposition of the Astri onds in rumen. Studied Trit Tretments T1(control) T2 dd 70 μl LO T3 dd 140 μl LO T4 dd 280 μl LO Significnt Tle 7. Effect of linseed oil supplementtion y 0, 70, 140 nd 280 μl / kg dry mtter on the concentrtion of voltile ftty cids (cetic, utyric nd propionic cid mmol / L) for the 80% lflf hy with 20% concentrted feed in different lortory incution periods ± ± ± ±0.15 Acetic cid(mmol/l) ± ± ± ±0.03 N.S ± ± ± ± ± ± ± ± ± ± ± ±0.03 Std ± men concentrtion of voltile ftty cids propionic cid(mmol/l) Incution periods(hour) ± ± ± ±0.03 N.S Butyric cid(mmol/l) T1: control(without ddition), T2: Diet dding linseed oil y 70 micro liters / kg DM, T3: Diet Adding linseed oil y 140 micro liters / kg DM, T4: Diet Adding linseed oil y 280 micro liters / kg DM, N.S indictes not significnt, indictes significnt differences t the proility level(p <0.01) ± ± ± ±0.05 N.S ± ± ± ± ± ± ± ± ± ± ± ±0.03 N.S ± ± ± ± ± ± ± ±

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