Supplementary Data AtMYB12

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1 Supplementary Data AtMYB12 expression in tomato leads to large scale differential modulation in transcriptome and flavonoid content in leaf and fruit tissues Ashutosh Pandey 1,$,#, Prashant Misra 1,$,#, Dharmendra Choudhary 2, Reena Yadav 1, Ridhi Goel 1, Sweta Bhambhani 1, Indraneel Sanyal 1, Ritu Trivedi 2,*, Prabodh Kumar Trivedi 1,* 1 Council of Scientific and Industrial Research-National Botanical Research Institute (CSIR- NBRI), Rana Pratap Marg, Lucknow , INDIA 2 CSIR-Central Drug Research Institute (CSIR-CDRI), Endocrinology Division, Jankipuram Extension, Sitapur Road, Lucknow , INDIA $ Present address (AP): National Agri-Food Biotechnology Institute (NABI), Mohali , Punjab, INDIA $ Present address (PM): CSIR-Indian Institute of Integrative Medicine (IIIM), Canal Road, Jammu , INDIA #: Contributed equally to this study *Authors for correspondence PKT: prabodht@hotmail.com, prabodht@nbri.res.in RT: ritu_trivedi@nbri.res.in Running title: AtMYB12 differentially modulates flavonoid biosynthesis in tomato fruit and leaf

with CaMV35S forward and gene specific reverse primers.

2 Supplementary Figure 1. Molecular characterization of transgenic lines. (a) Confirmation of the transgene by PCR amplification of genomic DNA with CaMV35S forward and gene specific reverse primers. (b) Expression analysis of transgene through semiquantitative RT- PCR using RNA from leaves.

Supplementary Figure 2. Effect of AtMYB12 expression on anthocyanin, polyphenol and flavonoid content in transgenic tomato lines as compared to WT.

(b) Quantification of total polyphenolic content using methanolic extracts. Quantification has been carried out by gallic acid equivalent.

3 Supplementary Figure 2. Effect of AtMYB12 expression on anthocyanin, polyphenol and flavonoid content in transgenic tomato lines as compared to WT. (a) Reduction in anthocyanin pigmentaion on stem and petiole in tomato transgenic line in comparison to wild type (WT) leaves. (b) Quantification of total polyphenolic content using methanolic extracts. Quantification has been carried out by gallic acid equivalent. (c) Quantification of total flavonoid content using methanolic extracts by quercetin equivalent. 1, 2 and 3 represent independent tomato transgenic lines expressing AtMYB12.

Supplementary Figure 3. Quantitative expression analysis of the structural genes upregulated in leaves of AtMYB12-expressing tomato transgenic lines.

1, 2 and 3 represent transgenic line 1, line 2 and line 3, respectively.

4 Supplementary Figure 3. Quantitative expression analysis of the structural genes upregulated in leaves of AtMYB12-expressing tomato transgenic lines. Expression of structural genes of phenylpropanoid pathway/flavonoid pathway in leaves of the WT and transgenic tomato lines. 1, 2 and 3 represent transgenic line 1, line 2 and line 3, respectively. PAL, Phenylalanine amonia lyase; CHI, Chalcone isomerase; CHS, Chalcone synthase; FLS, Flavonol synthase; F3H, Flavonone-3-hydroxylase; F3 H, flavonoid 3 -hydroxylase; 4CL, 4- coumaroyl CoA ligase; ANS, Anthocyanidine synthase HCT, Hydroxycinnamoyl transferase; GT, Glucosyltransferase.

5 Supplementary Figure 4. Distribution of common and unique genes with modulated expression among leaves and fruits of transgenic and WT tomato plants. (a) up-regulated unigenes and (b) down-regulated unigenes.

Secondary plant product stress responses a response to temperature stimulus response to heat response to wounding Down Up response to stress response to stimulus response to radiation response to

6 Secondary plant product stress responses a response to temperature stimulus response to heat response to wounding Down Up response to stress response to stimulus response to radiation response to light stimulus response to external stimulus response to abiotic stimulus flavonoid metabolic process flavonoid biosynthetic process secondary metabolic process phenylpropanoid metabolic process phenylpropanoid biosynthetic process b Percentage of gene count Supplementary Figure 5. Gene ontology of differentially regulated genes and functional network predicted. (a) Solanum lycopersicum homologues of the differentially-regulated genes in AtMYB12-expressing tomato leaves were identified and grouped with respect to their predicted role in different processes using agrigo (level 4). (b) Solanum lycopersicum homologues of the differentially expressed genes in AtMYB12-expressing tomato were identified to construct interactive network using Pathway Studio (Ariadne Genomics, USA).

Supplementary Figure 6. Validation of differential expression of genes involved in different processes in leaves of AtMYB12-expressing tomato transgenic lines.

2.1); CROT, Crotenase (Solyc12g011160.1.1); CYT450, Cytochrome P450 (Solyc10g083700.2.1); ERF, Ethylene-responsive transcription factor 4 (Solyc07g053740.1.1); GST, Glutathione S-transferase (Solyc10g084400.

7 Supplementary Figure 6. Validation of differential expression of genes involved in different processes in leaves of AtMYB12-expressing tomato transgenic lines. 1, 2 and 3 represent line 1, line 2 and line 3 respectively.acp-1-aminocyclopropane-1-carboxylate synthase (Solyc08g ); AQ, Aquaporin (Solyc12g ); AUX, Auxin efflux carrier family protein (Solyc02g ); CROT, Crotenase (Solyc12g ); CYT450, Cytochrome P450 (Solyc10g ); ERF, Ethylene-responsive transcription factor 4 (Solyc07g ); GST, Glutathione S-transferase (Solyc10g ); PDH, Prephenate dehydratase (Solyc06g ).

(a) Differential expression of genes involved in flavonoid biosynthesis.

(c) Differential expression of genes encoding involved in phytohormone

(d) Differential expression of genes involved in aromatic amino acid

(e) Differential expression of genes involved in glutathione S transferase.

8 a b c d e Supplementary Figure 7. Differential expression of genes in leaves and fruits of tomato plants. (a) Differential expression of genes involved in flavonoid biosynthesis. (b) Differential expression of genes encoding glycosyltransferases. (c) Differential expression of genes encoding involved in phytohormone biosynthesis, signalling and response. (d) Differential expression of genes involved in aromatic amino acid biosynthesis. (e) Differential expression of genes involved in glutathione S transferase. The locus ids of various regulatory genes were searched from microarray data. These probe set ids were used for annotation using different databases. Heat maps denoting digital expression by using MeV version 4.3 program. WT and TR represent wild type and transgenic line.

Supplementary Figure 8. Differential expression of genes encoding enzymes involved in carbohydrate, lipid and organic acid metabolism in leaves and fruits of tomato plants.

9 Supplementary Figure 8. Differential expression of genes encoding enzymes involved in carbohydrate, lipid and organic acid metabolism in leaves and fruits of tomato plants. The locus ids of various regulatory genes were searched from microarray data. These probe set ids were used for annotation using different databases. Heat maps denoting digital expression by using MeV version 4.3 program. WT and TR represent wild type and transgenic line.

10 Table 1: List of up-regulated ( two-fold) genes in leaves of AtMYB12-expressing tomato lines S.No Locus ID SOLGEN locus id Annotation in SOLGEN 1 Les S1_at Solyc05g Chalcone synthase 2 Les S1_at Solyc09g Chalcone synthase 3 Les S1_at Solyc11g Anthocyanidin synthase 4 Les S1_at Solyc02g Flavanone 3-hydroxylase 5 LesAffx S1_at Solyc02g Dihydroflavonol-4-reductase 6 LesAffx S1_at Solyc02g Dihydroflavonol-4-reductase 7 LesAffx S1_at Solyc01g Ethylene responsive transcription factor 2b 8 LesAffx S1_at Solyc08g Ethylene-responsive transcription factor 10 9 LesAffx S1_at Solyc05g Chalcone--flavonone isomerase 10 LesAffx A1_at Solyc02g Dihydroflavonol-4-reductase 11 LesAffx S1_at Solyc12g coumarate-coa ligase 12 LesAffx A1_at Solyc02g Glycosyltransferase 13 LesAffx S1_at Solyc05g IAA-amino acid hydrolase 14 LesAffx S1_at Solyc07g Auxin-induced protein-like 15 LesAffx S1_at Solyc10g UDP flavonoid 3-O-glucosyltransferase (Fragment) 16 LesAffx A1_at Solyc03g Cinnamoyl-CoA reductase-like protein 17 LesAffx S1_at Solyc04g UDP-glucosyltransferase 18 Les S1_at Solyc02g Indole-3-acetic acid-amido synthetase GH LesAffx S1_at Solyc04g Ethylene responsive transcription factor 2b 20 Les S1_at Solyc02g aminocyclopropane-1-carboxylate oxidase 3 21 Les A1_at Solyc03g coumarate CoA ligase 22 LesAffx S1_at Solyc08g Ethylene-responsive transcription factor 1 23 RPTR-Les-U _s_at No Hit 24 Les A1_at No Hit 25 LesAffx S1_at Solyc08g Protein TIFY 5A 26 Les S1_at Solyc02g Proline dehydrogenase 27 Les S1_at Solyc01g Legumin 11S-globulin 28 Les S1_at Solyc03g Pectinesterase 29 LesAffx S1_at Solyc06g Ovarian cancer-associated gene 2 protein homolog 30 Les S1_at Solyc11g Proteinase inhibitor II 31 Les S1_at Solyc04g Monooxygenase FAD-binding 32 Les S1_at Solyc02g Proline dehydrogenase 33 Les A1_a_at No Hit 34 Les S1_x_at Solyc11g Calmodulin-like protein 35 LesAffx A1_at Solyc08g Laccase Les.13.1.S1_at Solyc04g cytochrome P450" 37 Les S1_a_at Solyc11g Calmodulin-like protein 38 Les S1_at Solyc09g Adenosine kinase 39 LesAffx S1_at Solyc02g Zinc finger AN1 domain-containing stress-associated protein Les S1_at Solyc03g Ammonium transporter 41 Les S1_s_at No Hit 42 Les S1_at Solyc06g Metallothionein-like protein type 2 43 LesAffx S1_at No Hit 44 Les A1_at No Hit 45 Les S1_at Solyc06g Bifunctional polymyxin resistance arna protein 46 Les S1_at Solyc07g Xyloglucan endotransglucosylase/hydrolase 7 47 LesAffx S1_at Solyc01g Gibberellin 2-oxidase 48 Les S1_at Solyc01g Receptor-like protein kinase At3g Les A1_at No Hit 50 LesAffx S1_at Solyc03g C4-dicarboxylate transporter/malic acid transport family protein 51 Les S1_at No Hit 52 Les S1_at Solyc04g Unknown Protein 53 Les A1_at No Hit 54 Les S1_at Solyc03g PPPDE peptidase domain-containing protein 1 55 Les S1_at Solyc01g Uncharacterized oxidoreductase Mb LesAffx S1_at Solyc08g ATP binding / serine-threonine kinase 57 Les S1_at Solyc10g Myb family transcription factor (Fragment) 58 LesAffx S1_at Solyc07g Solanesyl diphosphate synthase 59 Les S1_at Solyc03g CRT binding factor 2 60 LesAffx S1_at Solyc04g Hydrolase alpha/beta fold family protein expressed 61 Les A1_at No Hit 62 Les S1_at Solyc02g Xyloglucan endotransglucosylase/hydrolase 2 63 Les S1_at Solyc03g Major facilitator superfamily domain containing protein 5 64 Protease inhibitor/seed storage/lipid transfer protein family protein Les S1_at Solyc03g LesAffx S1_at Solyc04g Os03g protein (Fragment) 66 LesAffx A1_at Solyc01g COBRA-like protein

11 67 Les A1_at No Hit 68 Les S1_at Solyc03g Xyloglucan endotransglucosylase/hydrolase 9 69 LesAffx S1_at Solyc05g Ribose-5-phosphate isomerase 70 Les S1_at Solyc02g Glycine rich protein 71 LesAffx S1_at Solyc01g Peptide methionine sulfoxide reductase msrb 72 LesAffx S1_at Solyc09g Acid phosphatase-like protein 73 Les S1_s_at Solyc04g Xyloglucan endotransglucosylase/hydrolase Les S1_at Solyc03g Lipoxygenase 75 LesAffx S1_at Solyc06g Methyltransferase like 7A 76 Les S1_a_at Solyc03g Phytoene synthase 1" 77 Les A1_at Solyc09g F-box family protein 78 LesAffx S1_at Solyc02g Peroxidase 1 79 LesAffx S1_at Solyc04g Ovarian cancer-associated gene 2 protein homolog 80 Les S1_at Solyc04g Phenylcoumaran benzylic ether reductase 81 Les S1_at Solyc12g Os07g protein (Fragment) 82 LesAffx S1_at Solyc07g Solanesyl diphosphate synthase 83 Les S1_at Solyc04g Carboxyl methyltransferase 84 Les S1_at Solyc01g Phospholipase D 85 Les S1_at Solyc12g Glyceraldehyde-3-phosphate dehydrogenase B 86 Les A1_at No Hit 87 LesAffx S1_at Solyc03g GCN5-related N-acetyltransferase 88 Les A1_at No Hit 89 LesAffx S1_at Solyc05g Cytidyltransferase-related 90 LesAffx S1_at Solyc06g Prephenate dehydrogenase family protein 91 LesAffx S1_at No Hit 92 LesAffx S1_at Solyc02g CBS domain containing protein 93 Les S1_at Solyc04g Xyloglucan endotransglucosylase/hydrolase Les A1_at Solyc04g Carboxyl methyltransferase 95 Les S1_at Solyc10g Late elongated hypocotyl and circadian clock associated-1-like" 96 LesAffx S1_at Solyc06g Conserved transmembrane protein 97 Les S1_at Solyc12g Glyceraldehyde-3-phosphate dehydrogenase B 98 Les S1_at Solyc02g Pectinesterase 99 LesAffx S1_at Solyc03g Oxalate oxidase-like germin LesAffx S1_at Solyc02g cdna clone J100026I16 full insert sequence 101 Les S1_at Solyc07g Xyloglucan endotransglucosylase/hydrolase LesAffx S1_at Solyc02g Zinc transporter protein 103 Les S1_at Solyc09g Ribulose-1 5-bisphosphate carboxylase/oxygenase activase LesAffx S1_at Solyc06g Bcl-2-associated athanogene-like protein

12 Table 2: List of down-regulated ( two-fold) genes in leaves of AtMYB12-expressing tomato lines S.No. Locus ID SOLGEN locus id Annotation in SOLGEN 1 LesAffx S1_at Solyc03g GDSL esterase/lipase At3g Les A1_at No Hit 3 Les A1_at No Hit 4 Les S1_a_at Solyc06g Unknown Protein 5 Les A1_at No Hit 6 Les S1_at Solyc02g B12D-like protein 7 Les A1_at No Hit 8 Les S1_at Solyc01g Cytochrome P450" 9 Les S1_at Solyc02g Cellular retinaldehyde-binding/triple function C-terminal 10 Les S1_at Solyc08g Osmotin-like protein (Fragment) 11 LesAffx S1_at Solyc10g Anthocyanidin synthase 12 Les A1_at No Hit 13 Les A1_at No Hit 14 LesAffx S1_at Solyc09g Os01g protein (Fragment) 15 Les A1_at No Hit 16 Les S2_at No Hit 17 LesAffx S1_at Solyc08g Heat stress transcription factor A3 18 Les S1_at Solyc03g Heat shock protein 19 Les A1_at No Hit 20 Les S1_at Solyc11g class IV heat shock protein 21 LesAffx S1_at Solyc10g Pyruvate decarboxylase 22 LesAffx S1_at Solyc01g LRR receptor-like serine/threonine-protein kinase, RLP" 23 Les S1_at Solyc04g Prostaglandin E synthase 3 24 LesAffx S1_at Solyc03g Nudix hydrolase 1 25 Les S1_at Solyc01g Unknown Protein 26 Les A1_at No Hit 27 LesAffx S1_at Solyc09g class I heat shock protein 3 28 LesAffx S1_at Solyc04g Pathogenesis-related protein-like protein 29 Les S1_at Solyc03g Kunitz trypsin inhibitor 30 Les S1_at Solyc06g Unknown Protein 31 Les S1_x_at Solyc00g Cysteine proteinase inhibitor 32 Les A1_at No Hit 33 Les S1_at Solyc05g Heat shock protein 34 LesAffx S1_at Solyc08g Anthocyanidin synthase (Fragment) 35 LesAffx S1_at Solyc04g class I heat shock protein 3 36 Les S1_at Solyc08g Auxin responsive protein 37 Les A1_at No Hit 38 Les S1_at Solyc07g aminocyclopropane-1-carboxylate oxidase 39 Les A1_at No Hit 40 Les S1_at Solyc11g Xyloglucan endotransglucosylase/hydrolase 9 41 Les S1_at Solyc07g Non-symbiotic hemoglobin protein

13 Table 3: List of up-regulated ( two-fold) genes in fruits of AtMYB12-expressing tomato lines S.No Locus ID SOLGEN locus id Annotation in SOLGEN 1 Les S1_at Solyc12g Aquaporin 2 Les S1_at Solyc08g aminocyclopropane-1-carboxylate synthase 3 Les S1_at Solyc09g Glutathione S-transferase-like protein 4 Les S1_at Solyc02g Indole-3-acetic acid-amido synthetase GH3.8 5 LesAffx S1_at Solyc08g UDP-glucose salicylic acid glucosyltransferase 6 Les S1_at Solyc05g Ethylene responsive transcription factor 1a 7 LesAffx S1_at Solyc12g Glucosyltransferase 8 Les S1_at Solyc02g Ethylene-responsive transcription factor 2 9 Les A1_at Solyc11g AMINOCYCLOPROPANE-1-CARBOXYLATE OXIDASE-like protein 10 LesAffx S1_at Solyc12g Glucosyltransferase 11 Les A1_at Solyc02g WRKY transcription factor LesAffx S1_at Solyc03g BHLH transcription factor-like protein 13 LesAffx S1_at Solyc08g UDP-glucose glucosyltransferase 14 Les S1_at Solyc10g Glutathione S-transferase 15 LesAffx S1_at Solyc06g Prephenate dehydrogenase family protein 16 LesAffx S1_at Solyc02g Auxin efflux carrier family protein 17 Les S1_at Solyc05g hydroxyphenylpyruvate dioxygenase 18 LesAffx S1_at Solyc10g Cytochrome P450" 19 LesAffx S1_at Solyc02g Cytochrome P450" 20 Les S1_at Solyc07g Ethylene-responsive transcription factor 4 21 LesAffx S1_at Solyc06g Prephenate dehydratase 22 LesAffx S1_at Solyc10g Cytochrome P450" 23 Les S1_at Solyc08g Polyphenol oxidase 24 Les S1_at Solyc05g hydroxyphenylpyruvate dioxygenase 25 Les S1_at Solyc01g Glutathione S-transferase 26 Les S1_at Solyc01g UDP-glucosyltransferase family 1 protein 27 LesAffx S1_at Solyc09g aminocyclopropane-1-carboxylate oxidase-like protein 28 LesAffx S1_at Solyc05g Chalcone--flavonone isomerase 29 Les S1_at Solyc06g Ethylene responsive transcription factor 1b 30 LesAffx S1_at Solyc12g Enoyl-CoA-hydratase 31 Les S1_at Solyc09g Phenylalanine ammonia-lyase 32 LesAffx S1_at Solyc01g UDP-glucosyltransferase family 1 protein 33 Les S1_at Solyc03g Kunitz trypsin inhibitor 34 Les S1_at No Hit 35 Les S1_at Solyc01g Plant viral-response family protein 36 RPTR-Les-U _s_at No Hit 37 Les S1_s_at Solyc03g Ribulose bisphosphate carboxylase small chain 38 Les S1_at Solyc09g Heat stress transcription factor A3 39 Les A1_at No Hit 40 Les S1_at Solyc10g Heavy metal-associated domain containing protein expressed 41 Les S1_at Solyc09g Proteinase inhibitor I 42 Les S1_at Solyc01g Cyclin 43 Les S1_at No Hit 44 Les S1_at Solyc10g Aquaporin 45 Les S1_at Solyc08g Expansin-like protein 46 LesAffx A1_at Solyc07g Beta-glucosidase 47 Les A1_a_at Solyc03g Chlorophyll a-b binding protein 3C-like 48 Les S1_at Solyc09g Proteinase inhibitor I 49 Les A1_x_at Solyc03g Chlorophyll a-b binding protein 3C-like 50 Les A1_at Solyc09g F-box family protein 51 LesAffx S1_at Solyc03g Unknown Protein 52 Les S1_at Solyc09g Aldo/keto reductase family protein 53 Les S1_at Solyc05g Protein disulfide isomerase 54 Les S1_s_at Solyc09g Unknown Protein 55 Les S1_at Solyc02g Chlorophyll a/b binding protein 56 Les S1_x_at Solyc02g Chlorophyll a/b binding protein 57 LesAffx S1_at Solyc01g Xenotropic and polytropic retrovirus receptor 58 Les S1_at Solyc01g Xylanase inhibitor (Fragment) 59 Les S1_at Solyc11g Alcohol dehydrogenase 60 Les A1_at Solyc10g Aquaporin 61 Les A1_at No Hit 62 Les S1_at Solyc09g Proteinase inhibitor I 63 Les S1_a_at Solyc11g Proteinase inhibitor 64 LesAffx A1_at Solyc00g ATP synthase subunit a 65 Les S1_a_at Solyc11g Calmodulin-like protein 66 LesAffx S1_at Solyc09g Pyrimidine 5&apos-nucleotidase 67 Les S1_at No Hit

14 68 Les S1_at No Hit 69 LesAffx S1_at Solyc00g ATPase subunit 8 70 LesAffx S1_at Solyc02g Thaumatin-like protein 71 Les S1_at Solyc02g Ribulose bisphosphate carboxylase small chain 72 LesAffx S1_at Solyc07g Heat stress transcription factor A3 73 LesAffx S1_at Solyc08g Heat stress transcription factor A3 74 LesAffx S1_at Solyc02g Inositol trisphosphate 5-phosphatase-like protein 75 LesAffx S1_at Solyc01g Cell number regulator Les S1_at Solyc02g Carbonic anhydrase 77 LesAffx S1_at Solyc09g Heat shock protein 1 78 LesAffx S1_at Solyc01g Unknown Protein 79 Les S1_at Solyc05g Unknown Protein 80 Les A1_at No Hit 81 Les A1_at Solyc07g Metallocarboxypeptidase inhibitor 82 Les A1_at No Hit 83 Les S1_at Solyc03g Glyoxalase/bleomycin resistance protein/dioxygenase 84 Les S1_at Solyc09g Unknown Protein 85 Les S1_s_at Solyc10g Palmitoyltransferase PFA4 86 LesAffx S1_at Solyc06g Bcl-2-associated athanogene-like protein 87 Les A1_at No Hit 88 Les S1_at Solyc10g Arginine decarboxylase 89 Les S1_s_at Solyc07g Glutathione S-transferase-like protein 90 Les S1_at Solyc03g Proteinase inhibitor II 91 LesAffx S1_at Solyc10g F-box protein PP2-B1 92 LesAffx A1_at Solyc01g Serine/threonine protein kinase-like 93 LesAffx S1_at Solyc01g Glyoxalase/bleomycin resistance protein/dioxygenase 94 Les S1_at Solyc07g Endoribonuclease L-PSP family protein 95 LesAffx S1_at Solyc03g Chaperone protein dnaj 96 Les S1_at Solyc11g class IV heat shock protein 97 Les A1_at Solyc04g Pheophorbide a oxygenase 98 Les S1_at Solyc04g Calcium-dependent protein kinase 2 99 Les S1_at Solyc12g Chlorophyll a-b binding protein 13, chloroplastic 100 Les.13.1.S1_at Solyc04g cytochrome P450" 101 Les S1_x_at Solyc11g Calmodulin-like protein 102 Les S1_at Solyc03g Aluminum-induced protein-like 103 LesAffx S1_at Solyc06g Short-chain dehydrogenase/reductase family protein 104 LesAffx S1_at Solyc08g Unknown Protein 105 LesAffx S1_at Solyc01g Xylanase inhibitor (Fragment) 106 Les A1_at No Hit 107 LesAffx S1_at Solyc08g ChaC cation transport regulator-like Les S1_at Solyc12g Chlorophyll a-b binding protein 37, chloroplastic 109 LesAffx S1_at Solyc12g TBC1 domain family member LesAffx S1_at Solyc01g SRC2 homolog (Fragment) 111 Les S1_at Solyc11g DNA cross-link repair 1B-like protein 112 Les S1_at No Hit 113 LesAffx S1_at Solyc01g Hydrolase-like 114 Les A1_at No Hit 115 LesAffx S1_at Solyc03g Unknown Protein 116 Les S1_a_at No Hit 117 Les S1_at Solyc04g Lipase-like protein 118 Les S1_at Solyc07g Nuclear transcription factor Y subunit B Les S1_at Solyc03g Kunitz-type protease inhibitor 120 LesAffx S1_at Solyc08g ChaC cation transport regulator-like LesAffx S1_a_at Solyc03g AT1G52630-like protein (Fragment) 122 LesAffx S1_at Solyc02g Strictosidine synthase family protein 123 Les S1_at Solyc02g MYB transcription factor (Fragment) 124 LesAffx S1_at Solyc02g class I heat shock protein 125 Les S1_s_at Solyc06g Phosphatase 126 Les S1_s_at Solyc06g Sex-linked protein 9 (Fragment) 127 LesAffx S1_at Solyc08g WRKY transcription factor Les S1_s_at Solyc06g Sex-linked protein 9 (Fragment) 129 Les A1_at No Hit 130 LesAffx S1_at No Hit 131 Les S1_at Solyc06g WRKY transcription factor Les S1_at No Hit 133 LesAffx S1_at Solyc04g Alpha/beta hydrolase fold protein 134 Les A1_at No Hit 135 Les S1_at Solyc03g Transcription regulatory protein SNF5 136 Les A1_at No Hit 137 LesAffx S1_at Solyc02g Strictosidine synthase family protein 138 LesAffx S1_at Solyc06g Glutathione S-transferase 139 LesAffx S1_at Solyc02g Glycerophosphodiester phosphodiesterase gde1 140 Les S1_at Solyc09g Unknown Protein 141 Les A1_at No Hit 142 Les S1_at Solyc10g Arginine decarboxylase

15 143 Les S1_at Solyc05g Chlorophyll a-b binding protein 6A, chloroplastic 144 Les S1_at No Hit 145 Les S1_at Solyc06g Sex-linked protein 9 (Fragment) 146 Les S1_at Solyc08g Photosystem I reaction center subunit 147 Les S1_at No Hit 148 Les S1_at No Hit 149 Les A1_at No Hit 150 Les A1_at No Hit 151 Les S1_at Solyc03g Protease inhibitor/seed storage/lipid transfer protein family protein 152 LesAffx A1_at No Hit 153 LesAffx S1_at Solyc03g AT1G52630-like protein (Fragment) 154 LesAffx S1_at Solyc04g TMV response-related protein 155 LesAffx S1_at Solyc05g Auxin-independent growth protein (Fragment) 156 Les S1_at Solyc03g Unknown Protein 157 LesAffx A1_at Solyc08g Anamorsin homolog 158 Les S1_at Solyc07g Xyloglucan endotransglucosylase/hydrolase Les S1_at No Hit 160 LesAffx S1_at Solyc02g MYB transcription factor 161 Les S1_at Solyc09g Threonine ammonia-lyase biosynthetic 162 Les A1_at Solyc10g Ferredoxin I 163 Les S1_at Solyc07g Ubiquitin fusion degradation protein LesAffx S1_at Solyc04g Ethylene-responsive transcription factor LesAffx S1_at Solyc10g Unknown Protein 166 Les A1_at No Hit 167 LesAffx S1_at Solyc01g Unknown Protein 168 Les S1_at Solyc10g Subtilisin-like protease 169 Les S1_at Solyc03g Chlorophyll a-b binding protein P4, chloroplastic 170 Les S1_at Solyc08g Stress-induced protein sti1-like protein 171 LesAffx S1_at Solyc06g Short-chain dehydrogenase/reductase family protein 172 Les S1_at Solyc06g Auxin responsive protein 173 Les S1_at No Hit 174 LesAffx A1_at Solyc02g Glycerophosphodiester phosphodiesterase gde1 175 LesAffx S1_at Solyc09g class I heat shock protein LesAffx S1_at Solyc02g Chorismate mutase Les A1_at No Hit 178 LesAffx.1.1.S1_at Solyc01g Agmatinase 179 Les S1_at No Hit 180 Les S1_at Solyc12g Photosystem II 5 kda protein, chloroplastic 181 Les A1_at No Hit 182 Les.84.1.S1_at Solyc12g Leucyl aminopeptidase 183 LesAffx S1_at Solyc06g Wound-induced protein RLK, Receptor like protein, putative resistance protein with an antifungal domain" LesAffx S1_at Solyc02g LesAffx S1_at No Hit 186 Les S1_at Solyc01g Glutamine synthetase 187 LesAffx A1_at Solyc04g Aspartate/glutamate/uridylate kinase family protein 188 Les S1_at Solyc03g Non-symbiotic hemoglobin LesAffx S1_at Solyc12g GRAS family transcription factor 190 Les A1_s_at No Hit 191 LesAffx S1_at Solyc10g LRR receptor-like serine/threonine-protein kinase, RLP" 192 LesAffx S1_at Solyc02g Macrophage erythroblast attacher 193 Les A1_at Solyc09g Proteinase inhibitor I 194 Les A1_s_at Solyc09g Unknown Protein 195 Les S1_at Solyc11g Calpain-2 catalytic subunit 196 Les S1_a_at No Hit 197 LesAffx S1_at Solyc05g F-box protein PP2-B1 198 Les A1_at Solyc02g MYB transcription factor (Fragment) 199 Les S1_at Solyc01g DNA polymerase 200 LesAffx S1_at Solyc01g Actin depolymerizing factor Les S1_at Solyc09g Unknown Protein 202 Les S1_at Solyc00g H-ATPase 203 Les S1_at Solyc04g Glyceraldehyde-3-phosphate dehydrogenase 204 Les S1_at Solyc04g Os01g protein (Fragment) 205 Les A1_at No Hit 206 LesAffx S1_at Solyc06g Prephenate dehydratase 207 LesAffx S1_at Solyc08g Os02g protein (Fragment) 208 LesAffx S1_at Solyc01g UBX domain protein LesAffx S1_at Solyc05g Poly polymerase catalytic domain containing protein expressed 210 Les S1_a_at No Hit 211 Les S1_at Solyc06g Integrin-linked kinase-associated serine/threonine phosphatase 2C 212 Les S1_at Solyc07g Saccharopine dehydrogenase (NAD(+) L-glutamate-forming) 213 Les S1_at Solyc10g Glutathione S-transferase 214 Les S1_at Solyc08g WRKY transcription factor Les S1_at No Hit 216 Les S1_at Solyc01g Cysteine-rich extensin-like protein-4

16 217 LesAffx S1_at No Hit 218 LesAffx S1_at Solyc05g Protein phosphatase-2c 219 LesAffx A1_at Solyc01g Unknown Protein 220 LesAffx S1_at Solyc03g Dof zinc finger protein LesAffx S1_at Solyc06g Cytochrome b5 reductase 222 Les S1_at Solyc09g Unknown Protein 223 LesAffx S1_at Solyc05g Chaperone protein dnaj LesAffx S1_at Solyc04g Phosphatidylserine synthase Les S1_at Solyc03g Pyridoxal biosynthesis lyase pdxs 226 Les S1_at Solyc06g Metallothionein-like protein type LesAffx S1_at Solyc02g Abhydrolase domain-containing protein FAM108B1 228 Les.13.1.S1_at Solyc04g cytochrome P450" 229 Les S1_at Solyc01g Zinc finger protein 230 Les A1_at Solyc11g ORF16-lacZ fusion protein 231 LesAffx S1_at Solyc01g Survival of motor neuron-related-splicing factor Les S1_at Solyc03g Chlorophyll a-b binding protein 3C-like 233 Les S1_at Solyc02g GDSL esterase/lipase At5g Les S1_at Solyc06g Os02g protein (Fragment) 235 LesAffx S1_at Solyc11g Zinc finger family protein 236 Les S1_at No Hit 237 Les S1_at Solyc04g NAC domain protein 238 Les A1_at No Hit 239 LesAffx A1_at Solyc03g Acid phosphatase 240 Les A1_at No Hit 241 Les S1_at No Hit 242 Les S1_at Solyc02g Plasma membrane associated protein 243 LesAffx S1_at Solyc07g Cotton fiber expressed protein Les S1_at Solyc07g Saccharopine dehydrogenase (NAD(+) L-glutamate-forming) 245 LesAffx S1_at Solyc01g Ubiquitin-conjugating enzyme E2 E3 246 LesAffx S1_at Solyc08g BHLH transcription factor 247 Les S1_at No Hit 248 Les A1_at No Hit 249 LesAffx S1_at Solyc00g Cytochrome c oxidase subunit LesAffx S1_at Solyc08g Zinc finger A20 and AN1 domain-containing stress-associated protein Les A1_at Solyc02g Peptidase trypsin-like serine and cysteine proteases (Fragment) 252 Les S1_at No Hit 253 LesAffx S1_at Solyc10g Response regulator Les S1_at Solyc09g PPPDE peptidase domain containing 2a 255 LesAffx S1_at Solyc02g Glycerophosphodiester phosphodiesterase gde1 256 LesAffx S1_at Solyc03g Unknown Protein 257 Les S1_at Solyc09g Ethylene responsive transcription factor 1a 258 Les A1_at No Hit 259 LesAffx S1_at Solyc01g Integral membrane protein 260 LesAffx S1_at Solyc05g RNA-binding protein Luc7-like LesAffx S1_at Solyc11g NADH-quinone oxidoreductase subunit 262 LesAffx S1_at Solyc02g Pantetheine-phosphate adenylyltransferase 263 LesAffx S1_at Solyc05g Poly polymerase catalytic domain containing protein expressed 264 Les S1_at Solyc03g Cellulose synthase 265 Les S1_at Solyc06g At1g72390-like protein (Fragment) 266 Les S1_a_at Solyc10g Ethylene-responsive transcription factor LesAffx A1_at Solyc04g Chaperone protein dnaj LesAffx S1_at Solyc10g Coiled-coil domain-containing protein 90B mitochondrial 269 LesAffx S1_at Solyc08g F-box family protein 270 Les S1_at Solyc06g Asparagine synthase (Glutamine-hydrolyzing) 271 LesAffx S1_at Solyc08g Avr9/Cf-9 rapidly elicited protein LesAffx S1_at Solyc00g Ring H2 finger protein 273 Les S1_at Solyc03g Rhodanese-like family protein-like protein (Fragment) 274 LesAffx S1_at Solyc02g Zinc finger AN1 domain-containing stress-associated protein Les A1_at No Hit 276 LesAffx S1_at Solyc00g ATP synthase subunit a 277 LesAffx S1_at Solyc07g FAD-dependent oxidoreductase family protein 278 Les S1_at Solyc10g Chlorophyll a-b binding protein 8, chloroplastic 279 Les S1_at Solyc07g aminocyclopropane-1-carboxylate oxidase 280 Les A1_at Solyc09g Unknown Protein 281 LesAffx S1_at Solyc07g Potassium channel tetramerization domain-containing protein 282 Les S1_at No Hit 283 Les A1_at No Hit 284 Les S1_at Solyc08g aminocyclopropane-1-carboxylate synthase 285 Les S1_at Solyc08g Phosphoribulokinase/uridine kinase 286 LesAffx S1_at Solyc02g Aldehyde dehydrogenase LesAffx S1_at Solyc02g Lrr, resistance protein fragment" 288 LesAffx S1_at Solyc01g Os05g protein (Fragment) 289 Les S1_s_at Solyc03g Ribulose bisphosphate carboxylase small chain 290 Les S1_at Solyc05g Acidic chitinase 291 LesAffx S1_at Solyc08g Zinc finger A20 and AN1 domain-containing stress-associated protein 7

17 292 Les A1_at No Hit 293 Les A1_at No Hit 294 Les S1_at Solyc07g Chlorophyll a-b binding protein 4, chloroplastic 298 Les S1_at Solyc09g Universal stress protein family protein 296 Les S1_at Solyc08g Zinc finger and SCAN domain containing 29 (Predicted) 297 LesAffx S1_at Solyc07g Decarboxylase family protein 298 Les S1_s_at Solyc09g Cell wall protein 299 Les S1_at Solyc03g MtN3-like protein 300 LesAffx S1_at Solyc07g Uncharacterized secreted protein 301 LesAffx S1_at Solyc01g Siroheme synthase 302 Les S1_at Solyc06g Heat shock protein 90 (Fragment) 303 Les S1_at Solyc12g Chlorophyll a-b binding protein 37, chloroplastic 304 LesAffx S1_at Solyc02g Protein serine/threonine kinase 305 LesAffx S1_s_at No Hit 306 Les A1_at No Hit 307 Les A1_at No Hit 308 Les S1_at Solyc01g ATPase AAA family protein expressed 309 Les A1_at No Hit 310 Les S1_at Solyc06g Vacuolar protein sorting-associated protein Les S1_at Solyc06g SET domain protein SUVR2 312 Les A1_at Solyc04g Water-stress inducible protein 3 (Fragment) 313 Les A1_at Solyc09g Unknown Protein 314 LesAffx S1_at Solyc01g Heavy metal-associated domain containing protein expressed 315 LesAffx S1_at Solyc06g N-acetyltransferase 316 LesAffx S1_at Solyc06g Mitochondrial import inner membrane translocase subunit TIM LesAffx S1_at Solyc01g Unknown Protein 318 Les S1_at Solyc06g Serine carboxypeptidase LesAffx S1_at Solyc07g NAC domain protein IPR LesAffx S1_at Solyc08g Avr9/Cf-9 rapidly elicited protein LesAffx S1_at Solyc10g MYB transcription factor (Fragment) 322 Les S1_at Solyc02g Pectinesterase 323 Les S1_at Solyc11g Mitogen-activated protein kinase 324 Les A1_at Solyc03g Glyoxal oxidase 325 Les A1_at No Hit 326 LesAffx A1_at Solyc01g UBX domain protein LesAffx S1_at Solyc03g Os05g protein (Fragment) 328 Les S1_at No Hit 329 LesAffx S1_at Solyc04g U-box domain-containing protein 330 Les S1_at Solyc01g Eukaryotic translation initiation factor 3 subunit B 331 Les A1_at No Hit 332 Les A1_at No Hit 333 Les S1_at Solyc04g Senescence-associated protein 334 Les S1_at Solyc01g Receptor-like kinase 335 LesAffx S1_at Solyc12g Dual specificity protein phosphatase Les A1_at No Hit 337 LesAffx A1_at Solyc10g Activator of heat shock protein ATPase homolog LesAffx S1_at Solyc03g Lipase-like protein 339 Les S1_at Solyc06g SNAP25 homologous protein SNAP Les S1_at Solyc04g Cell division protein kinase LesAffx S1_at Solyc03g Zinc finger protein-like protein 342 Les S1_at Solyc06g heat shock protein 343 Les S1_a_at Solyc01g Chitinase (Fragment) 344 Les S1_at Solyc12g TBC1 domain family member LesAffx S1_at Solyc04g UV excision repair protein RAD LesAffx S1_at Solyc01g Digalactosyldiacylglycerol synthase 2, chloroplastic 347 LesAffx S1_at Solyc02g Octanoyltransferase 348 LesAffx S1_at Solyc03g Hydrolase alpha/beta fold family protein 349 LesAffx S1_at Solyc09g Ras-related protein Rab LesAffx S1_at Solyc03g IBR finger domain protein 351 LesAffx S1_at Solyc01g Serine/threonine protein kinase-like 352 LesAffx S1_at Solyc07g ATP-dependent RNA helicase 353 Les S1_at Solyc06g Photosystem I reaction center subunit XI 354 LesAffx S1_at Solyc03g hydroxy-3-oxopropionate reductase 355 LesAffx S1_at Solyc04g MYB transcription factor 356 Les A1_at Solyc02g Succinate dehydrogenase iron-sulfur protein 357 LesAffx S1_at Solyc04g cdna clone J100026I16 full insert sequence 358 Les A1_at Solyc01g Unknown Protein 359 LesAffx S1_at Solyc07g UPF0431 protein C1orf66 homolog 360 Les S1_at No Hit 361 Les S1_at Solyc10g RING finger protein Les S1_at Solyc03g Alpha-hydroxynitrile lyase 363 LesAffx A1_at Solyc06g Nuclear SR-like RNA binding protein 364 Les S1_at Solyc01g Knotted-like homeobox protein 365 LesAffx A1_at Solyc12g F-box protein PP2-B1 366 LesAffx A1_at Solyc07g Decarboxylase family protein

18 367 Les S1_at Solyc08g Xenotropic and polytropic retrovirus receptor 368 Les S1_at Solyc06g Photosystem II reaction center W protein 369 Les A1_at No Hit 370 Les A1_at No Hit 371 LesAffx S1_at Solyc08g Globin 372 LesAffx S1_at Solyc02g C17orf95 protein (Fragment) 373 Les S1_x_at Solyc06g Phosphatase 374 Les S1_at Solyc07g SWIb domain-containing protein 375 Les A1_x_at No Hit 376 Les S1_at Solyc04g Unknown Protein 377 Les S1_at Solyc11g MYB transcription factor 378 LesAffx S1_at Solyc07g Serine/threonine protein kinase 379 LesAffx A1_at Solyc04g TMV response-related protein 380 Les S1_at Solyc07g ATP synthase I-like protein 381 Les A1_at No Hit 382 LesAffx A1_at Solyc01g COBRA-like protein 383 LesAffx A1_at Solyc11g Charged multivesicular body protein Les S1_at Solyc06g cdna clone C11 full insert sequence 385 LesAffx A1_at Solyc03g LYR motif-containing protein Les S1_at No Hit 387 Les S1_at Solyc05g Regulator of chromosome condensation RCC1 domain-containing protein 388 Les.47.1.S1_at Solyc02g MADS-box transcription factor 389 LesAffx S1_at Solyc03g E3 ubiquitin-protein ligase MARCH3 390 LesAffx S1_at Solyc07g Purine permease family protein 391 Les A1_at No Hit 392 Les S1_at No Hit 393 Les S1_at Solyc03g Polygalacturonase 394 Les S1_at Solyc09g PPPDE peptidase domain containing 2a 395 Les A1_at No Hit 396 LesAffx S1_at Solyc04g Tubby-like F-box protein Les S1_at No Hit 398 LesAffx S1_at Solyc03g Oleosin 399 Les S1_at Solyc01g Peroxidase 400 Les S1_at Solyc12g Exonuclease DNA polymerase III epsilon subunit family 401 LesAffx A1_at Solyc01g U-box domain-containing protein 402 LesAffx S1_at Solyc03g Unknown Protein 403 LesAffx S1_at Solyc09g Gibberellin receptor GID1L2 404 Les S1_at Solyc01g BZIP transcription factor 405 Les A1_at No Hit 406 Les S1_at Solyc06g Mitochondrial import inner membrane translocase subunit TIM LesAffx S1_at Solyc11g OTU domain-containing protein Les A1_at No Hit 409 LesAffx S1_at Solyc05g RING finger protein 410 LesAffx S1_at Solyc03g Ribulose bisphosphate carboxylase/oxygenase activase 411 LesAffx S1_at Solyc02g Splicing factor arginine/serine-rich Les S1_at Solyc05g Unknown Protein 413 LesAffx S1_at Solyc05g Unknown Protein 414 LesAffx S1_at Solyc03g RING finger protein Les A1_at No Hit 416 LesAffx S1_at Solyc03g Ring H2 finger protein 417 LesAffx S1_at Solyc01g U-box domain-containing protein 418 LesAffx S1_at Solyc04g Ras-related protein Rab LesAffx S1_at Solyc03g Hydrolase alpha/beta fold family protein 420 LesAffx S1_at Solyc01g AT4g33690/T16L1_180 (Fragment) 421 Les A1_at No Hit 422 Les S1_at No Hit 423 Les S1_at Solyc10g Chlorophyll a-b binding protein 7, chloroplastic 424 Les S1_at No Hit 425 Les S1_at Solyc01g Zinc finger A20 and AN1 domain-containing stress-associated protein LesAffx S1_at Solyc01g Os11g protein (Fragment) 427 LesAffx S1_at Solyc02g Ras-related protein Rab-2-A 428 Les S1_at Solyc10g Kelch-like protein Les S1_at Solyc10g Harpin-induced protein-like (Fragment) 430 Les A1_s_at Solyc09g Unknown Protein 431 Les S1_at Solyc07g L-lactate dehydrogenase 432 Les S1_at No Hit 433 LesAffx S1_at Solyc08g Short-chain dehydrogenase/reductase family protein 434 Les A1_at No Hit 435 LesAffx S1_at Solyc10g GDSL esterase/lipase At5g LesAffx S1_at Solyc11g Splicing factor 3B subunit Les S1_at Solyc09g Cobalt import ATP-binding protein CbiO Les S1_at Solyc02g Solute carrier family 2, facilitated glucose transporter member LesAffx S1_at Solyc07g RING finger protein 440 LesAffx A1_at Solyc05g Lipid phosphate phosphatase Les A1_at No Hit

19 442 LesAffx S1_at Solyc06g trna-dihydrouridine synthase 2-like protein 443 Les A1_at No Hit 444 Les A1_at No Hit 445 Les S1_at Solyc08g Unknown Protein 446 Les S1_at No Hit 447 LesAffx S1_at Solyc09g Short-chain dehydrogenase/reductase family protein 448 LesAffx A1_at Solyc10g CONSTANS-like zinc finger protein 449 Les A1_at Solyc12g Pectinesterase 450 Les S1_at Solyc01g Multiprotein bridging factor LesAffx S1_at Solyc12g F-box protein PP2-B1 452 Les S1_at Solyc05g Chloroplast photosystem II subunit X (Fragment) 453 Les S1_at Solyc10g Late elongated hypocotyl and circadian clock associated-1-like" 454 Les A1_at No Hit 455 LesAffx S1_at Solyc06g Golgi SNAP receptor complex member LesAffx S1_at Solyc07g MTD1 457 Les S1_at Solyc05g NAC domain transcription factor 458 Les S1_a_at No Hit 459 Les S1_at Solyc10g Heat shock protein Les A1_at No Hit 461 LesAffx S1_at Solyc07g Os06g protein (Fragment) 462 LesAffx S1_at No Hit 463 Les S1_at Solyc03g Guanylyl cyclase 464 LesAffx S1_at Solyc01g cdna clone J023121M11 full insert sequence 465 LesAffx A1_at Solyc01g S proteasome regulatory subunit 466 Les S1_at Solyc01g Vacuolar import and degradation protein VID LesAffx S1_at Solyc06g Transcription factor (Fragment) 468 Les S1_at Solyc03g Xyloglucan endotransglucosylase/hydrolase Les S1_at Solyc09g Selenium binding protein 470 LesAffx A1_at Solyc10g Eukaryotic translation initiation factor 4E 471 Les S1_at Solyc06g Protein tolb 472 LesAffx S1_at Solyc11g heat shock protein 473 Les S1_at Solyc12g Short-chain dehydrogenase/reductase family protein 474 Les S1_at Solyc05g Heat shock protein 475 Les S1_at Solyc01g S-adenosylmethionine decarboxylase proenzyme 476 Les A1_at No Hit 477 Les S1_at Solyc03g Heat shock protein 478 Les S1_at Solyc08g UDP-glucose 4-epimerase 479 LesAffx S1_at Solyc07g Yippee zinc-binding-like protein 480 Les S1_at Solyc01g SNARE associated Golgi protein 481 LesAffx S1_at Solyc08g Tasselseed2-like short-chain dehydrogenase/reductase (Fragment) 482 LesAffx S1_at Solyc01g Transporter major facilitator family 483 LesAffx A1_at Solyc06g Os11g protein (Fragment) 484 Les S1_at No Hit 485 Les S1_at Solyc03g Chlorophyll a-b binding protein P4, chloroplastic 486 Les S1_at Solyc12g ATP-dependent clp protease ATP-binding subunit 487 LesAffx A1_at Solyc10g SLT1 protein 488 LesAffx S1_at Solyc06g trna-dihydrouridine synthase 2-like protein 489 LesAffx S1_at Solyc04g Bet1-like protein At4g LesAffx S1_at Solyc05g Cytochrome P450" 491 Les A1_at No Hit 492 Les A1_at No Hit 493 Les S1_at Solyc08g Stress-induced protein sti1-like protein 494 LesAffx S1_at Solyc01g COBRA-like protein 495 Les S1_at Solyc01g Agmatinase 496 LesAffx S1_at Solyc11g Charged multivesicular body protein Les S1_at Solyc12g Protein phosphatase 2C 498 Les S1_at Solyc12g Aminotransferase-like protein 499 LesAffx S1_at No Hit 500 Les A1_at No Hit 501 LesAffx S1_at Solyc07g Oxalate oxidase-like germin LesAffx S1_at Solyc01g E3 ubiquitin-protein ligase MARCH6 503 Les S1_at Solyc12g Protochlorophyllide reductase 504 Les S1_at Solyc05g Unknown Protein 505 LesAffx S1_at Solyc01g Rubredoxin family protein 506 Les S1_at Solyc03g SPFH domain / Band 7 family protein 507 Les S1_at Solyc09g Cyclin dependent kinase inhibitor 508 LesAffx S1_at Solyc04g Prostaglandin E synthase 2-like 509 Les S1_at Solyc03g Transcription regulatory protein SNF5 510 LesAffx S1_at Solyc12g MADS box transcription factor 511 Les S1_s_at Solyc01g WD repeat-containing protein 512 Les S1_at No Hit 513 LesAffx S1_at Solyc04g U-box domain-containing protein 514 Les S1_at Solyc09g Tubby-like F-box protein LesAffx S1_at Solyc12g Density-regulated protein 516 LesAffx S1_at Solyc03g Cinnamoyl-CoA reductase-like protein

20 517 LesAffx A1_at Solyc04g Genomic DNA chromosome 5 P1 clone MCA Les A1_at Solyc01g MADS-box transcription factor Les S1_at Solyc06g SCF ubiquitin ligase skp1 component 520 LesAffx S1_at Solyc10g SLT1 protein 521 LesAffx S1_at Solyc03g MYB transcription factor 522 LesAffx A1_at Solyc06g Pseudo response regulator 523 LesAffx S1_at Solyc07g S proteasome non-atpase regulatory subunit Les A1_at No Hit 525 LesAffx A1_at Solyc01g Carbohydrate kinase YjeF related protein 526 LesAffx S1_at Solyc01g Heterogeneous nuclear ribonucleoprotein A3-like protein LesAffx A1_at No Hit 528 Les S1_at Solyc06g Transmembrane BAX inhibitor motif-containing protein LesAffx S1_at Solyc08g Zinc finger family protein 530 Les S1_at No Hit 531 Les S1_at Solyc09g Arginine/serine-rich splicing factor 532 LesAffx S1_at Solyc12g CHCH domain containing protein 533 LesAffx S1_at Solyc02g Cysteine-rich PDZ-binding protein 534 Les A1_at No Hit 535 LesAffx S1_at Solyc09g C2 domain-containing protein-like 536 LesAffx S1_at Solyc01g Zinc finger family protein 537 LesAffx S1_at Solyc02g Dof zinc finger protein 538 Les A1_at No Hit 539 LesAffx S1_at Solyc10g F-box protein SKIP Les.32.2.S1_a_at Solyc04g Cryptochrome 1a" 541 LesAffx S1_at Solyc06g Trans-acting transcriptional protein ICP0 542 Les S1_at Solyc04g ABC-1 domain protein 543 Les S1_at Solyc01g Mitochondrial carrier protein 544 LesAffx S1_at Solyc10g Elicitor-responsive protein Les S1_at Solyc05g Transcription elongation factor A protein Les A1_at No Hit 547 LesAffx S1_at Solyc10g Unknown Protein 548 LesAffx S1_at Solyc03g Abscisic acid receptor PYL8 549 Les S1_at Solyc02g ATP synthase gamma chain 550 LesAffx S1_at Solyc12g Pre-mRNA-splicing factor ini1 551 LesAffx S1_at Solyc12g Unknown Protein 552 Les S1_at Solyc07g Receptor-like protein kinase At5g Les A1_at No Hit 554 LesAffx S1_at Solyc05g YIPF5 555 LesAffx S1_at Solyc01g UPF0468 protein C16orf80 homolog 556 LesAffx S1_at No Hit 557 Les A1_at No Hit 558 LesAffx S1_at Solyc02g Speckle-type POZ protein 559 Les S1_at Solyc06g Genomic DNA chromosome 5 TAC clone K15C Les A1_at No Hit 561 Les A1_at No Hit 562 LesAffx S1_at Solyc01g Abscisic acid receptor PYL8 563 LesAffx S1_at Solyc08g Coiled-coil domain-containing protein Les S1_at Solyc07g Acetolactate synthase 565 Les S1_at Solyc08g Class II small heat shock protein Le-HSP LesAffx A1_at Solyc09g Phytochrome A-associated F-box protein 567 Les S1_at Solyc10g Kelch-like protein Les S1_at No Hit 569 Les S1_at Solyc04g Unknown Protein 570 LesAffx S1_at Solyc03g WD-40 repeat family protein 571 Les S1_at Solyc02g Glucan endo-1 3-beta-glucosidase LesAffx S1_at Solyc02g Coiled-coil domain-containing protein 109A 573 LesAffx S1_at Solyc01g Cytochrome b6-f complex subunit LesAffx A1_at Solyc01g Digalactosyldiacylglycerol synthase 2, chloroplastic 575 LesAffx S1_at Solyc02g Tafazzin 576 LesAffx S1_at Solyc02g CBS domain containing protein 577 Les S1_at Solyc07g Xyloglucan endotransglucosylase/hydrolase LesAffx S1_at Solyc02g class I heat shock protein 579 Les S1_at Solyc04g Sucrose transporter 580 Les S1_at Solyc03g Lipoxygenase 581 LesAffx S1_at Solyc09g Actin-depolymerizing factor Les A1_at No Hit 583 Les A1_at No Hit 584 LesAffx S1_at No Hit 585 LesAffx S1_at Solyc08g Unknown Protein 586 Les A1_at Solyc10g CWC15 homolog 587 Les A1_at No Hit 588 Les A1_at No Hit 589 Les A1_at No Hit 590 LesAffx S1_at Solyc11g Inner membrane ALBINO3-like protein 591 Les S1_at Solyc09g Ammonium transporter

WT siz1-2 siz1-3. WT siz1-2 siz1-3. -Pi, 0.05 M IAA. -Pi, 2.5 M NPA

WT siz1-2 siz1-3. WT siz1-2 siz1-3. -Pi, 0.05 M IAA. -Pi, 2.5 M NPA A WT siz1-2 siz1-3 B WT siz1-2 siz1-3 -Pi C +Pi WT siz1-2 siz1-3 D WT siz1-2 siz1-3 E +Pi, 0.05 M IAA -Pi, 0.05 M IAA WT siz1-2 siz1-3 WT siz1-2 siz1-3 F +Pi, 2.5 M NPA -Pi, 2.5 M NPA Supplemental Figure