mock! A3AC106S! A3BE255Q! 86.7! 90.1! 88.0! 89.8! 89.0!
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1 a A3A A3Bi7 A3Btok A3Bwh A3Blan mock V5 DAPI merge 5 µm b edited A3A A3Bi7 A3Btok A3Blan A3Bwh A3BF38L A3BW359L mock A3AC16S A3BE255Q HBV 3DPCR Td/ C Supplementary Figure 1. Cellular localization of A3B proteins and HBV editing. a) Confocal microscopy of V5 tagged A3A proteins performed in HeLa cells 24 hours post transfection. Nuclei are stained using DAPI. b) 3D-PCR analysis of HBV DNA editing by A3B proteins.
2 Supplementary Figure 2. A3B nudna editing and 5-methylcytidine deamination. a) Quail cmyc specific 3DPCR gels after QT6 cells transfections with A3 proteins along with UGI coding plasmid. Asterisks denote the samples that were molecularly cloned and sequenced. b) Mutation matrices of cmyc hyperedited sequences. The numbers below the matrices indicate the number of bases sequenced. c) 5 Dinucleotide analysis of the deamination context performed on DNA minus strand for cloned PCR products. * denote significant deviation from expected values (χ2 test, p<.5, n=18 for A3A, n=23 for A3Bi7). d) Selection of hypermutated 5Me-dC substituted HIV-1 sequences after co-transfection along with A3 coding plasmids in QT6 cells. e) Mutation matrices of HIV-1 hyperedited sequences. The numbers below the matrices indicate the number of bases sequenced. Data presented correspond to 3DPCR products obtained at 81.3 C. a b quail Myc Td/ C A3A A3Bi M * * A3A T C G A T 2 C G 9 A A3Bi7 T C G A T 1 C G 1 3 A d A3Btok A3Bwh A3Blan A3AC16S A3BE255Q A3A A3Bi7 A3Btok A3Blan mock 5Me-dC HIV-1 : 1 bp from 276 bp c Deamination context (%) n= 4864 bp n= 5888 bp % * * TpC CpC GpC ApC A3A A3Bi7 expected GAGCAGTTTTTTATCTCTCCTTTCTCCATCATCATTTCCCCGCTACTACTATTGGTATTACTACTATTGGTATTAGTAGCATTCCCCAAATCAGTGCACT...T.T.TT...T.TT..T..T...TTTT.T...T...T..T...TTTT...T......T.T.TT...T.TT..T..T...TTTT...T...T... A.A...A...AA...A......T.T.TT...T.TT..T..T...TTTT.T...TTTT...T...T......T.T.TT...T.TT..T..T...TT...T......T...T...T...T..T...T.TT...T.TT...T......T.T.TT...T.TT..T..T...TTTT...T...TTTT...T......T...T...T...TTT...TTTT...T...T.T....T...T.T.TT...T...TTTT...T...T......T...T...T...TTT...T.T......T...T...T..T..T...TT.T...TTT......T...T.T..T...T..T..T..T...TTTT...T...T...TT.T...T...T... * * e A3A T C G A A3Bi7 T C G A A3Btok T C G A A3Blan T C G A T T T 4 2 T 8 C 763 C 539 C 346 C G 27 G 63 G 17 G 1 18 A 1 4 A A 3 A n= 6825 bp n= 9955 bp n= 3864 bp n= 5796 bp
3 25K.44 25K 1 Mock 2K 15K 1K A3Btok 2K 15K 1K K V5 5K V5 γh2ax 25K 1 25K 1 A3AC16S 2K 15K 1K A3Blan 2K 15K 1K K 2 5K V5 γh2ax V5 γh2ax 25K 1 25K 1 A3A 2K 15K 1K A3Bwh 2K 15K 1K K 2 5K V γh2ax V5 γh2ax 25K 1 25K 1 A3Bi7 2K 15K 1K A3BE255Q 2K 15K 1K K 2 5K V5 γh2ax V5 γh2ax Supplementary figure 3a. FACS plots of γh2ax staining on V5 positive HeLa cells gated on V5 (pink) after A3 (2µg) transfection performed at 48 hours. Cells. Blue histograms represent γh2ax staining for A3AC16S catalytic mutant transfection used as negative control.
4 25K 25K 1 Mock 2K 15K 1K.149 A3Btok 2K 15K 1K K V5 5K V5 γh2ax A3AC16S 25K 2K 15K 1K A3Blan 25K 2K 15K 1K K 2 5K V5 γh2ax V5 γh2ax 25K 1 25K 1 A3A 2K 15K 1K A3Bwh 2K 15K 1K K 2 5K V γh2ax V5 γh2ax 25K 1 25K 1 A3Bi7 2K 15K 1K A3BE255Q 2K 15K 1K K 2 5K V5 γh2ax V5 γh2ax Supplementary figure 3b. FACS plots of γh2ax staining on V5 positive QT6 cells gated on V5 (pink) after A3 (2µg) transfection performed at 48 hours. Cells. Blue histograms represent γh2ax staining for A3AC16S catalytic mutant transfection used as negative control.
5 a A3AC16S A3A A3Bi7 A3Btok γh2ax γh2ax γh2ax γh2ax A3Blan A3Bwh A3BE255Q b % γh2ax in V * γh2ax γh2ax A3 + mock A3 + UGI γh2ax Supplementary Figure 4. FACS analysis of γh2ax-positive HeLa cells gated on V5 after A3 (1µg) cotransfection with mock (1µg) or UGI (1µg) coding plasmids at 48 hours. a) FACS plots of γh2ax staining on V5 positive cells. Red histograms represent γh2ax staining for transfections performed with 1µg of mock plasmid, blue histograms represent γh2ax staining for transfections performed with UGI coding plasmid. Error bars represent standard deviation from four independent transfections. Difference between mock transfected cells and UGI transfected cells for A3A was calculated using student test (*p=.375).
6 exon 5/8 AsnGlnGlyAsn*** A3A-UTR AATCAGGGAAACTGAAGGATGGGCCTCAGTCTCTAAGGAAGGCAGAGACCTGGGTTGAGCAGCAGAATAAAAGATCTTCTTCCAAGAAATGCAAACAGAC 1 A3B-UTR AATCAGGGAAACTGAAGGATGGGCCTCAGTCTCTAAGGAAGGCAGAGACCTGGGTTGAGCAGCAGAATAAAAGATCTTCTTCCAAGAAATGCAAACAGAC 1 **************************************************************************************************** A3A-UTR CGTTCACCACCATCTCCAGCTGCTCACAGACGCCAGCAAAGCAGTATGCTCCCGATCAAGTAGATTTTTAAAAAATCAGAGTGGGCCGGGCGCGGTGGCT 2 A3B-UTR CGTTCACCACCATCTCCAGCTGCTCACAGACACCAGCAAAGCAATGTGCTCCTGATCAAGTAGATTTTTTAAAAATCAGAGT ******************************* *********** * ****** **************** ************>>> ALU element A3A-UTR CACGCCTGTAATCCCAGCACTTTGGAGGCCAAGGCGGGTGGATCACGAGGTCAGGAGATCGAGACCATCCTGGCTAACACGGTGAAACCCTGTCTCTACT 3 A3B-UTR A3A-UTR AAAAATACAAAAAATTAGCCAGGCGTGGTGGCGGGCGCCTGTAGTCCCAGCTACTCTGGAGGCTGAGGCAGGAGAGTAGCGTGAACCCGGGAGGCAGAGC 4 A3B-UTR A3A-UTR TTGCGGTGAGCCGAGATTGCGCTACTGCACTCCAGCCTGGGCGACAGTACCAGACTCCATCTCAAAAAAAAAAAAACCAGACTGAATTAATTTTAACTGA 5 A3B-UTR CAATTAATTTTAATTGA 199 Alu element <<< ************ *** A3A-UTR AAATTTCTCTTATGTTCCAAGTACACAATAGTAAGATTATGCTCAATATTCTCAGAATAATTTTCAATGTATTAATGAAATGAAATGATAATTTGGCTTC 6 A3B-UTR AAATTTCTCTTATGTTCCAAGTGTACAAGAGTAAGATTATGCTCAATATTCCCAGAATAGTTTTCAATGTATTAATGAAG-----TGATTAATTGGCTCC 294 ********************** **** ********************** ******* ******************* **** * ****** * NM_12746, U3891 < A3A-UTR ATATCTAGACTAACACAAAATTAAGAATCTTCCATAATTGCTTTTGCTCAGTAACTGTGTCATGAATTGCAAGAGTTTCCACAAACACTAGCAAATGTGT 7 BC3183, AK24854 < BC53859 < U6183, U6184 < A3B-UTR ATATTTAGACTAATAAAACATTAAGAATCTTCCATAATTG TTTCCACAAACACTAGCAAATGTGT 359 **** ******** * ** ********************* ************************* A3A-UTR AGATGTCTTTCCTTGTGTAGCGGACCTGTAGCGGGGAAAGGTCACACAACATCCCTCTGGATCCAGAAAACTCAGCTAAACCTCACAGGAGAGGAACCTA 8 A3B-UTR AGATGTCTTTCCTTGTGTAGCGGACCTGTAGCTGGGAAAGGTCACACAACATCCCTCTGGATCCAGAAAACTCAGCTAAACCACACAGGAGAGGAACCTA 459 ******************************** ************************************************* ***************** A3A-UTR AATGCAGACCCCACCCTCACTCACAGAGCCCCGCCCACCCTCACTTACAGAGCCCCGGGCGCTGATTGG 869 A3B-UTR AATGCAGACCCCACCCTCACTCACAGAGCCCCGCCCACCCTCACTCACAGAGCCCCGGGCGCTGATTGG 528 ********************************************* *********************** Supplementary Figure 5. Sequence comparison of the 3 UTR encoded by exon 5 of A3A and exon 8 of A3B genes used in the study. An imperfect repeated sequence flanking the Alu element is underlined. A3A and A3B transcripts are identified by their accession numbers and their 3 ends denoted by arrowheads (<).
7 a Mock A3A- UTR A3A-UTR A3A A3A-UTR A3B 25K 25K K K 5.2 2K 15K 1K.173 2K 15K 1K 2K 15K 1K 2K 15K 1K 5K 5K 5K 5K HA HA HA HA γh2ax γh2ax γh2ax b DMSO PMA Etoposide γh2ax γh2ax γh2ax Supplementary figure 6 a) FACS plots of γh2ax staining on HA positive HeLa cells gated on HA (pink) after A3 (2µg) transfection performed at 48 hours. Cells. Blue histograms represent γh2ax staining for A3AC16S catalytic mutant transfection used as negative control. b) FACS plots of γh2ax staining of SKBR3 cells after treatment. Blue histograms represent γh2ax staining for DMSO treated cells used as negative control.
8 Plasmid Matrix Primers For : 5 -CACCATGAATCCACAGATCAGAAATCCGATGGAGC A3Blan pcdna3.1-a3b-ha Rev : 5 -GTTTCCCTGATTCTGGAGAAT For : 5 -CACCATGAATCCACAGATCAGAAATCCGATGGAGC A3Bi7 Puc57-A3Bi Rev : 5 -GTTTCCCTGATTCTGGAAGAGCAGGGGGGTTG A3A UTR A3AC16S A3A UTRA A3A UTRB Luc UTR Luc UTRA Luc UTRB A3Ai4 For : 5'-CACCATGGCTTATCCTTACGACGTGCCTGACTACGCCATGGAAGCCAGCCCAGCATCCGGGCCCAGACACTTG A3AC16S Rev : 5'-ACTGAGGCCCATCCTTCAGTTTCCCTGATTCTGGAAGAGCAG For : 5'-CACCATGGCTTATCCTTACGACGTGCCTGACTACGCCATGGAAGCCAGCCCAGCATCCGGGCCCAGACACTTG A3Ai4 Rev : 5'-ACTGAGGCCCATCCTTCAGTTTCCCTGATTCTGGAAGAGCAG For : 5'-AATCAGGGAAACTGAAGGATGGGCCTCAGTCTCTAAGGAAGG 293T gdna Rev : 5'-CCAATCAGCGCCCGGGGCTCTGTRAGTGAGGGTGGGCGGGGC For : 5'-CACCATGGCTTATCCTTACGACGTGCCTGACTACGCCATGGAAGCCAGCCCAGCATCCGGGCCCAGACACTTG A3Ai4 Rev : 5'-ACTGAGGCCCATCCTTCAGTTTCCCTGATTCTGGAAGAGCAG For : 5'-AATCAGGGAAACTGAAGGATGGGCCTCAGTCTCTAAGGAAGG 293T gdna Rev : 5'-CCAATCAGCGCCCGGGGCTCTGTRAGTGAGGGTGGGCGGGGC pgl4.5 For : 5'-CACCATGGAAGATGCCAAAAACATTAAGA [luc2/cmv/hygro] Rev : 5'-TTACACGGCGATCTTGCCGCCCTTCTTGGCCTT pgl4.5 For : 5'-CACCATGGAAGATGCCAAAAACATTAAGA [luc2/cmv/hygro] Rev : 5'-CCTTCCTTAGAGACTGAGGCCCATCCTTTACACGGCGATCTTGCCGCCCTTCTTGGCCTT For : 5'-AGGATGGGCCTCAGTCTCTAAGGAAGG A3A UTRA Rev : 5'-GTTTCCCTGATTCTGGAAGAGCAGGGGGGTTG pgl4.5 For : 5'-CACCATGGAAGATGCCAAAAACATTAAGA [luc2/cmv/hygro] Rev : 5'-GTTTCCCTGATTCTGGAAGAGCAGGGGGGTTG For : 5'-AGGATGGGCCTCAGTCTCTAAGGAAGG A3A UTRB Rev : 5'-CCTTCCTTAGAGACTGAGGCCCATCCTTTACACGGCGATCTTGCCGCCCTTCTTGGCCTT Supplementary table 1. Compendium of primers used for PCR cloning
9 Plasmid Matrix Primers A3Btok (T146K) A3Bi7 For : 5'-GCAGGAGCCCGCGTGAAGATCATGGACTATGAA Rev : 5 -TTCATAGTCCATGATCTTCACGCGGGCTCCTGC A3BiE255Q A3Bi7 For : 5 -TACGGCCGCCATGCGCAGCTGCGCTTCTTGGAC Rev : 5 -GTCCAAGAAGCGCAGCTGCGCATGGCGGCCGTA A3BF38L A3Bi7 For : 5'-GTGAGACTGCGCATCTTAGCTGCCCGCATCTAT Rev : 5'-ATAGATGCGGGCAGCTAAGATGCGCAGTCTCAC A3BW359L A3Bi7 For : 5'-TGTCCCTTCCAGCCCTTGGATGGACTAGAGGAG Rev : 5'-CTCCTCTAGTCCATCCAAGGGCTGGAAGGGACA Supplementary table 2. Compendium of primers used for mutagenesis
10 Primer Application Sequence Cycling conditions HBV out fwd HBV out rev HBV PCR HBV PCR 5'-AAGAGTYYTYTTATGTAAGACYTT 5'-CGCAAATATACATCGTATCCAT 95 C 5 min 35x (95 C 1 min, 5 C 3 sec, 72 C 5 min), 72 C 1 min HBV in fwd HBV in rev HBV 3DPCR HBV 3DPCR 5'-AAGTGCACACGGTYYGGCAGAT 5'-ATGGCTGCTARGCTGTGCTGCCAA 8-9 C 5 min 35x (8-9 C 1 min, 5 C 3 sec, 72 C 5 min), 72 C 1 min TP53 out fwd TP53 out rev TP53 PCR TP53 PCR 5 GAGCTGGACCTTAGGCTCCAGAAAGGACAA 5 -GCTGGTGTTGTTGGGCAGTGCTAGGAA 95 C 5 min 42x (95 C 3 sec, 6 C 3 sec, 72 C 2 min), 72 C 1 min TP53 in fwd TP53 in rev TP53 3DPCR 5 -TTCTCTTTTCCTATCCTGAGTAGTGGTAA TP53 3DPCR 5 -AAAGGTGATAAAAGTGAATCTGAGGCATAA C 5 min 4x (84-92 C 1 min, 58 C 3 sec, 72 C 2 min), 72 C 1 min HIV-1 out fwd HIV-1 out rev HIV-1 PCR HIV-1 PCR 5 -TGTACCCACAGACCCCAACCCACAA 5 -TTCCATTGAACGTCTTATTATTACA 95 C 5 min 3x (95 C 45 sec, 54 C 45 sec, 72 C 2 min), 72 C 1 min HIV-1 in fwd HIV-1 in rev HIV-1 3DPCR 5 -ATCAAAGCCTAAAGCCATGTGTAA HIV-1 3DPCR 5 -CAATAATGTATGGGAATTGGCTCAA 78-9 C 5 min 45x (78-9 C 1 min, 56 C 3 sec, 72 C 5 min), 72 C 1 min QT6 cmyc out fwd QT6 cmyc out rev cmyc PCR cmyc PCR 5 -GATGCCGCTCAGCGCCAGCCTCCCCAGCAA 5 -GCAGTCCTGGATGATGATGGATTTGACGAA 95 C 5 min 4x (95 C 1 min, 58 C 45 sec, 72 C 2 min), 72 C 1 min QT6 cmyc in fwd QT6 cmyc in rev cmyc 3DPCR 5 -TTCGAGGAGGAGGAGGAGAACTTCTA cmyc 3DPCR 5 -GATTCGTCGTCCGGGTCGCAGATGAA C 5 min 42x (88-95 C 1 min, 6 C 1 min, 72 C 2 min), 72 C 1 min Supplementary table 3. Compendium of primers and PCR conditions used for nested PCR/3DPCR amplifications
11 b 293T A3A- UTR A3A-UTRA3A A3A-UTRA3B Mock 293T A3A- UTR A3A-UTRA3A A3A-UTRA3B Mock kda Mock A3A A3AC16S A3Bi7 A3Btok A3Blan A3Bwh A3BE255Q a Ac$n Mock A3A A3AC16S A3Bi7 A3Btok A3Blan A3Bwh A3BE255Q kda V5 kda Ac$n HA Supplementary data 1. Uncropped scans of westerblots from a) Figure 1B, b) Figure 3C.
Nature Structural and Molecular Biology: doi: /nsmb Supplementary Figure 1
Supplementary Figure 1 Mutational analysis of the SA2-Scc1 interaction in vitro and in human cells. (a) Autoradiograph (top) and Coomassie stained gel (bottom) of 35 S-labeled Myc-SA2 proteins (input)
p = formed with HCI-001 p = Relative # of blood vessels that formed with HCI-002 Control Bevacizumab + 17AAG Bevacizumab 17AAG
A.. Relative # of ECs associated with HCI-001 1.4 1.2 1.0 0.8 0.6 0.4 0.2 0.0 ol b p < 0.001 Relative # of blood vessels that formed with HCI-001 1.4 1.2 1.0 0.8 0.6 0.4 0.2 0.0 l b p = 0.002 Control IHC:
Supplementary Fig. S1. Schematic diagram of minigenome segments.
open reading frame 1565 (segment 5) 47 (-) 3 5 (+) 76 101 125 149 173 197 221 246 287 open reading frame 890 (segment 8) 60 (-) 3 5 (+) 172 Supplementary Fig. S1. Schematic diagram of minigenome segments.
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IP: Flag a Mascot PTM Modified Mass Error Position Gene Names Score Score Sequence m/z [ppm] 81 MAT2A;AMS2;MATA2 35.6 137.28 _AAVDYQK(ac)VVR_ 595.83-2.28 b Pre-immu After-immu Flag- WT K81R WT K81R / Flag
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Supplementary Figure 1. Normal T lymphocyte populations in Dapk -/- mice. (a) Normal thymic development in Dapk -/- mice. Thymocytes from WT and Dapk -/- mice were stained for expression of CD4 and CD8.
Nature Medicine: doi: /nm.2109
HIV 1 Infects Multipotent Progenitor Cells Causing Cell Death and Establishing Latent Cellular Reservoirs Christoph C. Carter, Adewunmi Onafuwa Nuga, Lucy A. M c Namara, James Riddell IV, Dale Bixby, Michael
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TRAF6 ubiquitinates TGFβ type I receptor to promote its cleavage and nuclear translocation in cancer
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Relative SOD1 activity. Relative SOD2 activity. Relative SOD activity (Infected:Mock) + CP + DDC
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HIF-inducible mir-191 promotes migration in breast cancer through complex regulation of TGFβ-signaling in hypoxic microenvironment.
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HEK293FT cells were transiently transfected with reporters, N3-ICD construct and
Supplementary Information Luciferase reporter assay HEK293FT cells were transiently transfected with reporters, N3-ICD construct and increased amounts of wild type or kinase inactive EGFR. Transfections
(a) Schematic diagram of the FS mutation of UVRAG in exon 8 containing the highly instable
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a 24hpf c 48hpf d e 60hpf f g 72hpf h i j k ISV ISV Figure 1. Vascular integrity defects and endothelial regression in mutant emryos. (a,c,e,g,i) Bright-field and (,d,f,h,j) corresponding fluorescent micrographs
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Supplementary Figure 1. Genotyping strategies for Mcm3 +/+, Mcm3 +/Lox and Mcm3 +/- mice and luciferase activity in Mcm3 +/Lox mice. A.
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SUPPLEMENTARY INFORMATION doi:10.1038/nature11429 S1a 6 7 8 9 Nlrc4 allele S1b Nlrc4 +/+ Nlrc4 +/F Nlrc4 F/F 9 Targeting construct 422 bp 273 bp FRT-neo-gb-PGK-FRT 3x.STOP S1c Nlrc4 +/+ Nlrc4 F/F casp1
Tbk1-TKO! DN cells (%)! 15! 10!
a! T Cells! TKO! B Cells! TKO! b! CD4! 8.9 85.2 3.4 2.88 CD8! Tbk1-TKO! 1.1 84.8 2.51 2.54 c! DN cells (%)! 4 3 2 1 DP cells (%)! 9 8 7 6 CD4 + SP cells (%)! 5 4 3 2 1 5 TKO! TKO! TKO! TKO! 15 1 5 CD8
Supplementary Fig. 1. GPRC5A post-transcriptionally down-regulates EGFR expression. (a) Plot of the changes in steady state mrna levels versus
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Supplementary Materials for
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Supplementary Figure 1. Formation of the AA5x. a, Camera lucida drawing of embryo at 48 hours post fertilization (hpf, modified from Kimmel et al. Dev Dyn. 1995 203:253-310). b, Confocal microangiogram
SUPPLEMENTARY INFORMATION. Supplementary Figures S1-S9. Supplementary Methods
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Supplementary Figure 1. Ras V12 expression in the entire eye-antennal disc does not cause invasive tumours. a, Eye-antennal discs expressing Ras V12 in all cells (marked with GFP, green) overgrow moderately
m 6 A mrna methylation regulates AKT activity to promote the proliferation and tumorigenicity of endometrial cancer
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SUPPLEMENTARY INFORMATION. Supp. Fig. 1. Autoimmunity. Tolerance APC APC. T cell. T cell. doi: /nature06253 ICOS ICOS TCR CD28 TCR CD28
Supp. Fig. 1 a APC b APC ICOS ICOS TCR CD28 mir P TCR CD28 P T cell Tolerance Roquin WT SG Icos mrna T cell Autoimmunity Roquin M199R SG Icos mrna www.nature.com/nature 1 Supp. Fig. 2 CD4 + CD44 low CD4
p47 negatively regulates IKK activation by inducing the lysosomal degradation of polyubiquitinated NEMO
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Supplementary Figures
Supplementary Figures Supplementary Figure 1 Characterization of stable expression of GlucB and sshbira in the CT26 cell line (a) Live cell imaging of stable CT26 cells expressing green fluorescent protein
sequences of a styx mutant reveals a T to A transversion in the donor splice site of intron 5
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Supporting Information
Supporting Information Palmisano et al. 10.1073/pnas.1202174109 Fig. S1. Expression of different transgenes, driven by either viral or human promoters, is up-regulated by amino acid starvation. (A) Quantification
IP: anti-gfp VPS29-GFP. IP: anti-vps26. IP: anti-gfp - + +
FAM21 Strump. WASH1 IP: anti- 1 2 3 4 5 6 FAM21 Strump. FKBP IP: anti-gfp VPS29- GFP GFP-FAM21 tail H H/P P H H/P P c FAM21 FKBP Strump. VPS29-GFP IP: anti-gfp 1 2 3 FKBP VPS VPS VPS VPS29 1 = VPS29-GFP
Supplementary Information and Figure legends
Supplementary Information and Figure legends Table S1. Primers for quantitative RT-PCR Target Sequence (5 -> 3 ) Target Sequence (5 -> 3 ) DAB2IP F:TGGACGATGTGCTCTATGCC R:GGATGGTGATGGTTTGGTAG Snail F:CCTCCCTGTCAGATGAGGAC
Tel: ; Fax: ;
Tel.: +98 216 696 9291; Fax: +98 216 696 9291; E-mail: mrasadeghi@pasteur.ac.ir Tel: +98 916 113 7679; Fax: +98 613 333 6380; E-mail: abakhshi_e@ajums.ac.ir A Soluble Chromatin-bound MOI 0 1 5 0 1 5 HDAC2
Kidney. Heart. Lung. Sirt1. Gapdh. Mouse IgG DAPI. Rabbit IgG DAPI
a e Na V 1.5 Ad-LacZ Ad- 110KD b Scn5a/ (relative to Ad-LacZ) f 150 100 50 0 p = 0.65 Ad-LacZ Ad- c Heart Lung Kidney Spleen 110KD d fl/fl c -/- DAPI 20 µm Na v 1.5 250KD fl/fl Rabbit IgG DAPI fl/fl Mouse
EGFR shrna A: CCGGCGCAAGTGTAAGAAGTGCGAACTCGAGTTCGCACTTCTTACACTTGCG TTTTTG. EGFR shrna B: CCGGAGAATGTGGAATACCTAAGGCTCGAGCCTTAGGTATTCCACATTCTCTT TTTG
Supplementary Methods Sequence of oligonucleotides used for shrna targeting EGFR EGFR shrna were obtained from the Harvard RNAi consortium. The following oligonucleotides (forward primer) were used to
TITLE: The Role of hcdc4 as a Tumor Suppressor Gene in Genomic Instability Underlying Prostate Cancer
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Supplementary Information The fate of W chromosomes in hybrids between wild silkmoths, Samia cynthia ssp.: no role in sex determination and reproduction Atsuo Yoshido, Frantisek Marec, Ken Sahara Supplementary
293T cells were transfected with indicated expression vectors and the whole-cell extracts were subjected
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Zhu et al, page 1. Supplementary Figures
Zhu et al, page 1 Supplementary Figures Supplementary Figure 1: Visual behavior and avoidance behavioral response in EPM trials. (a) Measures of visual behavior that performed the light avoidance behavior
Supplementary material Legends to Supplementary Figures Figure S1. Figure S2. Figure S3.
Supplementary material Legends to Supplementary Figures. Figure S1. Expression of BICD-N-MTS fusion does not affect the distribution of the Golgi and endosomes. HeLa cells were transfected with GFP-BICD-N-MTS
Supplementary Figure 1: Expression of NFAT proteins in Nfat2-deleted B cells (a+b) Protein expression of NFAT2 (a) and NFAT1 (b) in isolated splenic
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1 a b Supplementary Figure 1. Effects of GSK3b knockdown on poly I:C-induced cytokine production. RAW264.7 cells stably expressing control shrna (Con) or GSK3b-specific shrna (sh- GSK3b) were stimulated
A. List of selected proteins with high SILAC (H/L) ratios identified in mass
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DOI:.38/ncb2822 a MTC02 FAO cells EEA1 b +/+ MEFs /DAPI -/- MEFs /DAPI -/- MEFs //DAPI c HEK 293 cells WCE N M C P AKT TBC1D7 Lamin A/C EEA1 VDAC d HeLa cells WCE N M C P AKT Lamin A/C EEA1 VDAC Figure