Supplementary Figure 1: TSLP receptor skin expression in dcssc. A: Healthy control (HC) skin with TSLP receptor expression in brown (10x
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1 Supplementary Figure 1: TSLP receptor skin expression in dcssc. A: Healthy control (HC) skin with TSLP receptor expression in brown (10x magnification). B: Second HC skin stained for TSLP receptor in brown (10x magnification). C: dcssc skin with positive staining of TSLP receptor in epidermis and interstitial cells (10x magnification). D: Second dcssc skin with TSLP receptor stained in brown (10x magnification). E: Similar section shown on Figure 1D with TSLP expression stained in brown (20x magnification). F: TSLP receptor positive staining in the perivascular area of similar section shown on Figure 1D (20x magnification).
2 Supplementary Figure 2: Mouse skin gene expression (A and B) after treatment with IL-13, TSLP, and TGFβ by 7 days subcutaneous pump showing two IL-13 clusters with Arg-1 gene (C) and Chi3l3 (D and E) upregulation both confirmed by qpcr (n=03 in PBS, IL-13, and TGFβ groups; n=06 in TSLP group; 2 independent experiments). D: Chi3l3 mrna expression IL-13 pump vs. PBS; p< Data are expressed as the fold-change normalized to mrna expression in one PBS sample. Each bar represents the mean ± standard error of the mean. See Figure 2G for Arg-1 qpcr results. Green color means lower gene expression; red color means higher gene expression.
3 Supplementary Figure 3: Mouse skin gene expression after 7 days of TSLP treatment in IL13-/- mice. A: PAI-1 mrna expression. B: NOS2 mrna expression. C: Arg1 mrna expression. D: CCL2 mrna expression. Data are expressed as the fold-change normalized to mrna expression in one PBS sample. Each bar represents the mean ± standard error of the mean. N= 03 in each group. ns= no significant.
4 Supplementary Figure 4: Mouse skin gene expression after 7 days of TSLP treatment in IFNAR and IFNgamma-deficient mice. A: PAI-1 mrna expression. B: CXCL5 mrna expression. Data are expressed as the fold-change normalized to mrna expression in on PBS sample; n= 03 in each group. Each bar represents the mean ± standard error of the mean. N= 3 in each group. ns= non significant.
5 Supplementary Figure 5: Representative flow cytometry analysis of WT and TSLP-/- mouse immune cell subsets from (A) blood and (B) spleen. A: Blood cells were gated for monocytes (CD11b+ CD115 high) and granulocytes (CD11b+ CD115 int). Identities for these populations were confirmed using forward and side scatter (right panel). B: Spleen cells were gated for T cells (R1; CD3+ B220-), B cells (R2; B220+ CD3-), dendritic cells (R3; CD11chigh CD11bint), macrophages (R4, R5; CD11b high, CD11c int, side scatter low), and granulocytes (R4, R6; CD11b high, CD11c int, side scatter high).
6 Supplementary Figure Legends: Supplementary Figure 1: TSLP receptor skin expression in dcssc. A: Healthy control (HC) skin with TSLP receptor expression in brown (10x magnification). B: Second HC skin stained for TSLP receptor in brown (10x magnification). C: dcssc skin with positive staining of TSLP receptor in epidermis and interstitial cells (10x magnification). D: Second dcssc skin with TSLP receptor stained in brown (10x magnification). E: Similar section shown on Figure 1D with TSLP expression stained in brown (20x magnification). F: TSLP receptor positive staining in the perivascular area of similar section shown on Figure 1D (20x magnification). Supplementary Figure 2: Mouse skin gene expression (A and B) after treatment with IL-13, TSLP, and TGFβ by 7 days subcutaneous pump showing two IL-13 clusters with Arg-1 gene (C) and Chi3l3 (D and E) upregulation both confirmed by qpcr (n=03 in PBS, IL-13, and TGFβ groups; n=06 in TSLP group; 2 independent experiments). D: Chi3l3 mrna expression IL-13 pump vs. PBS; p< Data are expressed as the fold-change normalized to mrna expression in one PBS sample. Each bar represents the mean ± standard error of the mean. See Figure 2G for Arg-1 qpcr results. Green color means lower gene expression; red color means higher gene expression.
7 Supplementary Figure 3: Mouse skin gene expression after 7 days of TSLP treatment in IL13-/- mice. A: PAI-1 mrna expression. B: NOS2 mrna expression. C: Arg1 mrna expression. D: CCL2 mrna expression. Data are expressed as the fold-change normalized to mrna expression in one PBS sample. Each bar represents the mean ± standard error of the mean. N= 03 in each group. ns= no significant. Supplementary Figure 4: Mouse skin gene expression after 7 days of TSLP treatment in IFNAR and IFN-gamma-deficient mice. A: PAI-1 mrna expression. B: CXCL5 mrna expression. Data are expressed as the fold-change normalized to mrna expression in on PBS sample; n= 03 in each group. Each bar represents the mean ± standard error of the mean. N= 3 in each group. ns= non significant. Supplementary Figure 5: Representative flow cytometry analysis of WT and TSLP-/- mouse immune cell subsets from (A) blood and (B) spleen. A: Blood cells were gated for monocytes (CD11b+ CD115 high) and granulocytes (CD11b+ CD115 int). Identities for these populations were confirmed using forward and side scatter (right panel). B: Spleen cells were gated for T cells (R1; CD3+ B220-), B cells (R2; B220+ CD3-), dendritic cells (R3; CD11chigh CD11bint), macrophages (R4, R5; CD11b high, CD11c int, side scatter low), and granulocytes (R4, R6; CD11b high, CD11c int, side scatter high).
8
% of live splenocytes. STAT5 deletion. (open shapes) % ROSA + % floxed
Supp. Figure 1. a 14 1 1 8 6 spleen cells (x1 6 ) 16 % of live splenocytes 5 4 3 1 % of live splenocytes 8 6 4 b 1 1 c % of CD11c + splenocytes (closed shapes) 8 6 4 8 6 4 % ROSA + (open shapes) % floxed
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well for 2 h at rt. Each dot represents an individual mouse and bar is the mean ±
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