Supplemental Fig. 1. Relative mrna Expression. Relative mrna Expression WT KO WT KO RT 4 0 C
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1 Supplemental Fig. 1 A Hdac11 (ibat) n=4 n=4 n=4 n=4 n=4 n=4 n=4 n=4 WT KO WT KO WT KO WT KO RT 4 C RT 4 C Supplemental Figure 1. Hdac11 mrna is undetectable in KO adipose tissue. Quantitative PCR analysis of Hdac11 mrna expression in mouse ibat (A) and ingwat (B) tissues. Values represent means ±SEM. B Hdac11 (ingwat)
2 Supplemental Fig. Plasma Concentration (pg/ml) Epinephrine Norepinephrine n=4 WT n=4 n=4 n=4 KO WT KO Supplemental Figure. HDAC11 deficient mice have higher levels of circulating norepinephrine. Plasma catecholamine (epinephrine and norepinephrine) concentrations in 1 week-old male mice fed normal chow were determined by ELISA. Values for the graph represent means ±SEM; P<.5 vs. WT. Student s t-test was used for statistical analysis.
3 Supplemental Fig. 3 A Lenti-Control or Lenti-FLAG- HDAC11 (WT or H143A) B BRD4 (Co-IPed) Control WT H143A HEK93 Cells IP: FLAG IB: Endogenous BRD4 BRD4 (Input) FLAG-HD11 (Input) kda 5 15 Supplemental Figure 3. HDAC11 does not associate with BRD4. (A) Schematic representation of the HDAC11:BRD4 coimmunoprecipitation (IP) assay; IB, immunoblot. (B) Immunoblotting revealed the absence of BRD4 in HDAC11 immunoprecipitates. Total amounts of HDAC11 and endogenous BRD4 in homogenates were also determined (input).
4 Supplemental Fig. 4 A Lenti-Control or Lenti-FLAG- HDAC11 (H143A) HIB1B Brown Preadipocytes BAT Diff Medium (4 days) B Ucp1 Diff: + + Ctrl H143A C Pgc1α Diff: + + Ctrl H143A Supplementary Figure 4. Catalytically inactive HDAC11 fails to suppress thermogenic gene expression. (A) Schematic representation of the experiment. The H143A amino acid substitution renders HDAC11 catalytically inactive. (B-C) Quantitative PCR analysis of Ucp1 and Pgc1α mrna expression; n = 4 plates of cells/condition. Values for all graphs represent means ±SEM; P<.5 vs. Lenti-Control undifferentiated. Twoway ANOVA with Tukey s multiple comparisons test was employed.
5 Supplemental Fig. 5 A B Lenti-Control or Lenti-Myc- BRD (WT) or Lenti-Myc- BRD 1-6 -/+ Lenti-shHDAC11 HIB1B Brown Preadipocytes BAT Diff Medium (4 days) Ucp1 Diff: BRD WT: + + BRD 1-6: + shcontrol: shhdac11: + + # Supplementary Figure 5. BRD-mediated suppression of brown adipocyte differentiation requires association with HDAC11. (A) Schematic representation of the experiment. (B-C) Quantitative PCR analysis of Ucp1 and Pgc1α mrna expression; n = 4 plates of cells/condition. Values for all graphs represent means ±SEM; P<.5 vs. Lenti-Control undifferentiated; # P<.5 vs. Lenti-Control differentiated; P<.5 vs. Lenti-BRD WT differentiated. Two-way ANOVA with Tukey s multiple comparisons test was employed. C Pgc1α Diff: BRD WT: + + BRD 1-6: + shcontrol: shhdac11: + + #
6 Supplemental Fig. 6 A B Lenti-Control or Lenti-FLAG HDAC11 (WT or H143A) BAT Diff Medium (4 days) HIB1B Brown Preadipocytes IP Ac-H3K7 PCR Ucp1 Enhancer Enrichment Relative to IgG IP Chromatin Immunoprecipitation Diff: Ctrl # WT H143A Supplementary Figure 6. HDAC11 blunts H3K7 acetylation of a Ucp1 enhancer element. (A) Schematic representation of the chromatin immunoprecipitation (ChIP) experiment. (B) ChIP-qPCR analysis showing relative enrichment of H3K7 acetylation at the Ucp1 enhancer. Values for the graph represent means ±SEM; P<.5 vs. Control undifferentiated; # P<.5 vs. control differentiated. Two-way ANOVA with Tukey s multiple comparisons test was employed.
7 Supplemental Tables Supplemental Table 1: Primers for qpcr Target 5 oligo 3 oligo Ucp1 CCGAAACTGTACAGCGGTCT CCGAGAGAGGCAGGTGTTTC Pgc1 TGTGTGCTGTGTGTCAGAGT ACCAGAGCAGCACACTCTATG 3-AR GGAAGCTTGCTTGATCCCCA GCCGTTGCTTGTCTTTCTGG Hdac11 TTACAACCGCCACATCTACC GACATTCCTCTCCACCTTCTC Brd AGCTGGGTTTGCCGGATTAT GGACAATATCATCGGTGGGCT Ucp1 enhancer (ChIP) AGCGTCACAGAGGGTCAGTC GTGAGGCTGATATCCCCAGA Supplemental Table : Primers for cloning Target 5 oligo 3 oligo BRD (1-6) CCATCGATCTGCAAAACGTGA CTCCCCACAATAAG CCGCTGAGTTATAAAGCAGCACT GCCACCCCCAC HDAC11 (H143A) GGGGGTGGCTTCCACGCCTG CTCCAGCGACCGT ACGGTCGCTGGAGCAGGCGTGG AAGCCACCCCC HDAC11 (1-3) CCATCGATCTACACACAACCC AGCTGTACCAGCAT GCTCTAGATCAGGTCACCATAAG GATGGGCACCCG HDAC11 (1-5) CCATCGATCTACACACAACCC AGCTGTACCAGCAT GCTCTAGATCACAGGTGCTCCTG GAGGGATTTCTT HDAC11 (1-) CCATCGATCTACACACAACCC AGCTGTACCAGCAT GCTCTAGATCAGATGTACACACG CTTGTCGTCCAT Supplemental Table 3: Primers for genotyping Target 5 oligo 3 oligo HDAC11 WT TGCTGCCTGTGAGCCACTGC AGAATGGCTGTCTCCCTAGG HDAC11 KO TGCTGCCTGTGAGCCACTGC CCTTGGAATAGCATCTCAGG Supplemental Table 4: Patient characteristics Brown Adipose Tissue-Deep Neck Patient ID Diagnosis Age Gender BMI (years) BAT-3 Multinodular goiter/ follicular adenoma 81 Female 37.7 BAT-9 Multinodular goiter 49 Male 34.4 BAT-13 Papillary thyroid carcinoma 7 Female 4. BAT-18 Parathyroid adenoma 56 Female 19. BAT-19 Benign follicular nodules 39 Female 6.9 BAT- Parathyroid adenoma 63 Female 4.8 1
8 White Adipose Tissue-Abdominal subcutaneous Patient ID Age Gender BMI (years) Ori-3 49 Female 3.9 Ori Female 34.7 Ori-38 4 Female.3 Ori Male 3. Ori-7 3 Male.4 Ori-76 9 Female 33.9 Ori Female 37.9 Ori-79 7 Female 1.1 Ori-81 4 Female 1.1
Supplementary Table 2. Plasma lipid profiles in wild type and mutant female mice submitted to a HFD for 12 weeks wt ERα -/- AF-1 0 AF-2 0
Supplementary Table 1. List of specific primers used for gene expression analysis. Genes Primer forward Primer reverse Hprt GCAGTACAGCCCCAAAATGG AACAAAGTCTGGCCTGTATCCA Srebp-1c GGAAGCTGTCGGGGTAGCGTC CATGTCTTCAAATGTGCAATCCAT
GPR120 *** * * Liver BAT iwat ewat mwat Ileum Colon. UCP1 mrna ***
a GPR120 GPR120 mrna/ppia mrna Arbitrary Units 150 100 50 Liver BAT iwat ewat mwat Ileum Colon b UCP1 mrna Fold induction 20 15 10 5 - camp camp SB202190 - - - H89 - - - - - GW7647 Supplementary Figure
Supplementary Figure 1
Supplementary Figure 1 Supplementary Figure 1 Schematic depiction of the tandem Fc GDF15. Supplementary Figure 2 Supplementary Figure 2 Gfral mrna levels in the brains of both wild-type and knockout Gfral
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doi:10.1038/nature12652 Supplementary Figure 1. PRDM16 interacts with endogenous EHMT1 in brown adipocytes. Immunoprecipitation of PRDM16 complex by flag antibody (M2) followed by Western blot analysis
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DOI: 10.1038/ncb2211 a! mir-143! b! mir-103/107! let-7a! mir-144! mir-122a! mir-126-3p! mir-194! mir-27a! mir-30c! Figure S1 Northern blot analysis of mir-143 expression dependent on feeding conditions.
Supplementary Figure 1. DNA methylation of the adiponectin promoter R1, Pparg2, and Tnfa promoter in adipocytes is not affected by obesity.
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Supplementary Information mediates STAT3 activation at retromer-positive structures to promote colitis and colitis-associated carcinogenesis Zhang et al. a b d e g h Rel. Luc. Act. Rel. mrna Rel. mrna
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Supplemental Information Supplementary Table. Urinary and adipose tissue catecholamines in Tph +/+ and Tph / mice fed a high fat diet for weeks. Tph +/+ Tph / Analyte ewat ibat ewat ibat Urine (ng/ml)
Cell isolation. Spleen and lymph nodes (axillary, inguinal) were removed from mice
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(a) Significant biological processes (upper panel) and disease biomarkers (lower panel)
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SUPPLEMENTARY DATA. Nature Medicine: doi: /nm.4171
SUPPLEMENTARY DATA Supplementary Figure 1 a b c PF %Change - -4-6 Body weight Lean mass Body fat Tissue weight (g).4.3.2.1. PF GC iwat awat BAT PF d e f g week 2 week 3 NEFA (mmol/l) 1..5. PF phsl (Ser565)
S1a S1b S1c. S1d. S1f S1g S1h SUPPLEMENTARY FIGURE 1. - si sc Il17rd Il17ra bp. rig/s IL-17RD (ng) -100 IL-17RD
SUPPLEMENTARY FIGURE 1 0 20 50 80 100 IL-17RD (ng) S1a S1b S1c IL-17RD β-actin kda S1d - si sc Il17rd Il17ra rig/s15-574 - 458-361 bp S1f S1g S1h S1i S1j Supplementary Figure 1. Knockdown of IL-17RD enhances
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DOI: 10.1038/ncb2566 Figure S1 CDKL5 protein expression pattern and localization in mouse brain. (a) Multiple-tissue western blot from a postnatal day (P) 21 mouse probed with an antibody against CDKL5.
Supplementary Figure 1 IL-27 IL
Tim-3 Supplementary Figure 1 Tc0 49.5 0.6 Tc1 63.5 0.84 Un 49.8 0.16 35.5 0.16 10 4 61.2 5.53 10 3 64.5 5.66 10 2 10 1 10 0 31 2.22 10 0 10 1 10 2 10 3 10 4 IL-10 28.2 1.69 IL-27 Supplementary Figure 1.
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doi: 1.138/nature7221 Brown fat selective genes 12 1 Control Q-RT-PCR (% of Control) 8 6 4 2 Ntrk3 Cox7a1 Cox8b Cox5b ATPase b2 ATPase f1a1 Sirt3 ERRα Elovl3/Cig3 PPARα Zic1 Supplementary Figure S1. stimulates
1.5 ASK1KO fed. fasted 16 hrs w/o water. Fed. 4th. 4th WT ASK1KO N=29, 11(WT), ,5(ASK1KO) ASK1KO ASK1KO **** Time [h]
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7: 13: 19: 1: 7: 151117 a 151117 4th 4th b c RQ.95 KO.9.85.8.75.7 light dark light dark.65 7: 19: 7: 19: 7: Means ± SEM, N=6 RQ 1..9.8.7.6.6 KO CL (-) CL (+) ibat weight ratio (/body weight) [%].5.4.3.2.1
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VO (ml kg - min - ) VCO (ml kg - min - ) Respiratory exchange ratio Energy expenditure (cal kg - min - ) Locomotor activity (x count) Body temperature ( C) Relative mrna expression TA Sol EDL PT Heart
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18s AAACGGCTACCACATCCAAG CCTCCAATGGATCCTCGTTA. 36b4 GTTCTTGCCCATCAGCACC AGATGCAGCAGATCCGCAT. Acc1 AGCAGATCCGCAGCTTG ACCTCTGCTCGCTGAGTGC
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-. -. SUPPLEMENTARY INFORMATION DOI: 1.1/ncb86 a WAT-1 WAT- BAT-1 BAT- sk-muscle-1 sk-muscle- mir-133b mir-133a mir-6 mir-378 mir-1 mir-85 mir-378 mir-6a mir-18 mir-133a mir- mir- mir-341 mir-196a mir-17
Nature Genetics: doi: /ng Supplementary Figure 1
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Relative expression ERE-luciferase activity activity (pmole/min) activity (pmole/min) activity (pmole/min) activity (pmole/min) MCF-7 KPL-3C ZR--1 BT-474 T47D HCC15 KPL-1 HBC4 activity (pmole/min) a d
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Supplementary Information and Figure legends
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Supplementary Figure 1 a
Supplementary Figure a Normalized expression/tbp (A.U.).6... Trip-br transcripts Trans Trans Trans b..5. Trip-br Ctrl LPS Normalized expression/tbp (A.U.) c Trip-br transcripts. adipocytes.... Trans Trans
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Kidney. Heart. Lung. Sirt1. Gapdh. Mouse IgG DAPI. Rabbit IgG DAPI
a e Na V 1.5 Ad-LacZ Ad- 110KD b Scn5a/ (relative to Ad-LacZ) f 150 100 50 0 p = 0.65 Ad-LacZ Ad- c Heart Lung Kidney Spleen 110KD d fl/fl c -/- DAPI 20 µm Na v 1.5 250KD fl/fl Rabbit IgG DAPI fl/fl Mouse
Supplementary Figure S1 Supplementary Figure S2
Supplementary Figure S A) The blots shown in Figure B were qualified by using Gel-Pro analyzer software (Rockville, MD, USA). The ratio of LC3II/LC3I to actin was then calculated. The data are represented
Supplement Figure S1. Real Time PCR analysis of mrna levels of C/EBPα and PU.1 in wild type (WT) and NQO1-null (NQO1-/-) mice.
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Supplementary Figure 1. MAT IIα is Acetylated at Lysine 81.
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A B16F1 s.c. Lung LN Distant lymph nodes Colon B B16F1 s.c. Supplementary Figure 1. Deletion of Smad3 prevents B16F1 melanoma invasion and metastasis in a mouse s.c. tumor model. Highly invasive growth
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RAW264.7 cells stably expressing control shrna (Con) or GSK3b-specific shrna (sh-
1 a b Supplementary Figure 1. Effects of GSK3b knockdown on poly I:C-induced cytokine production. RAW264.7 cells stably expressing control shrna (Con) or GSK3b-specific shrna (sh- GSK3b) were stimulated
for six pairs of mice. (b) Representative FACS analysis of absolute number of T cells (CD4 + and
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doi: /nature14508 Rappsilber et al.
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T H E J O U R N A L O F C E L L B I O L O G Y
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293T cells were transfected with indicated expression vectors and the whole-cell extracts were subjected
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Supplemental Figure 1. (A) Western blot for the expression of RIPK1 in HK-2 cells treated with or without LPS (1 µg/ml) for indicated times.
Supplemental Figure 1. (A) Western blot for the expression of RIPK1 in HK-2 cells treated with or without LPS (1 µg/ml) for indicated times. Western blots shown are representative results from 3 independent
Supplemental information
Carcinoemryonic antigen-related cell adhesion molecule 6 (CEACAM6) promotes EGF receptor signaling of oral squamous cell carcinoma metastasis via the complex N-glycosylation y Chiang et al. Supplemental
and follicular helper T cells is Egr2-dependent. (a) Diagrammatic representation of the
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Supporting Information
Supporting Information Charalambous et al. 10.1073/pnas.1406119111 SI Experimental Procedures Serum and Tissue Biochemistry. Enzymatic assay kits were used for determination of plasma FFAs (Roche), TAGs
File Name: Supplementary Information Description: Supplementary Figures and Supplementary Tables. File Name: Peer Review File Description:
File Name: Supplementary Information Description: Supplementary Figures and Supplementary Tables File Name: Peer Review File Description: Primer Name Sequence (5'-3') AT ( C) RT-PCR USP21 F 5'-TTCCCATGGCTCCTTCCACATGAT-3'
Figure 6: TERT regulates MYC half-life and ubiquitination.
TERT or IgG as indicated. For the western blots, representative images of n= independent experiments are shown. Student s t-test was used, and * indicates p
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Supplementary Figure 1 Differential expression of mirnas from the pri-mir-17-92a locus. (a) The mir-17-92a expression unit in the third intron of the host mir-17hg transcript. (b,c) Impact of knockdown
Supplementary Figures
Supplementary Figures Supplementary Figure 1. Confirmation of Dnmt1 conditional knockout out mice. a, Representative images of sorted stem (Lin - CD49f high CD24 + ), luminal (Lin - CD49f low CD24 + )
Supplemental Table 1. Plasma NEFA and liver triglyceride levels in ap2-hif1ako and ap2-hif2ako mice under control and high fat diets.
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Supplementary Figure 1. Genotyping strategies for Mcm3 +/+, Mcm3 +/Lox and Mcm3 +/- mice and luciferase activity in Mcm3 +/Lox mice. A.
Supplementary Figure 1. Genotyping strategies for Mcm3 +/+, Mcm3 +/Lox and Mcm3 +/- mice and luciferase activity in Mcm3 +/Lox mice. A. Upper part, three-primer PCR strategy at the Mcm3 locus yielding
SUPPLEMENTARY FIGURES
SUPPLEMENTARY FIGURES Figure S1. Effect of a HFD on the Acox gene expression in the livers of WT and IL-6 -/- mice. Expression of Acox in the livers of WT and IL-6 -/- mice fed STD or HFD determined through
Metabolic ER stress and inflammation in white adipose tissue (WAT) of mice with dietary obesity.
Supplementary Figure 1 Metabolic ER stress and inflammation in white adipose tissue (WAT) of mice with dietary obesity. Male C57BL/6J mice were fed a normal chow (NC, 10% fat) or a high-fat diet (HFD,
Nature Genetics: doi: /ng.3731
Supplementary Figure 1 Circadian profiles of Adarb1 transcript and ADARB1 protein in mouse tissues. (a) Overlap of rhythmic transcripts identified in the previous transcriptome analyses. The mouse liver
Supplemental Information. Differential Effects of EGFL6 on Tumor. versus Wound Angiogenesis
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Supplementary Figure 1. Blood glucose and insulin levels in mice during 4-day infusion.
Supplementary Figure 1. Blood glucose and insulin levels in mice during 4-day infusion. (A-B) WT and HT mice infused with saline or glucose had overlapping achieved blood glucose and insulin levels, necessitating
Supplementary Table 1. The primers used for quantitative RT-PCR. Gene name Forward (5 > 3 ) Reverse (5 > 3 )
770 771 Supplementary Table 1. The primers used for quantitative RT-PCR. Gene name Forward (5 > 3 ) Reverse (5 > 3 ) Human CXCL1 GCGCCCAAACCGAAGTCATA ATGGGGGATGCAGGATTGAG PF4 CCCCACTGCCCAACTGATAG TTCTTGTACAGCGGGGCTTG
* This work was supported, in whole or in part, by National Institutes of Health Grants HL076684, HL62984 (to N. L. W.), and ES (to S. H.
THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 286, NO. 31, pp. 27836 27847, August 5, 2011 Printed in the U.S.A. Histone Deacetylase 9 Is a Negative Regulator of Adipogenic Differentiation * S Received for
Histone demethylase JMJD1A coordinates acute and chronic adaptation to cold stress via thermogenic phospho-switch. Abe et al.
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Scaffold function of long noncoding RNA HOTAIR in protein ubiquitination
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Supplementary Figure 1. PD-L1 is glycosylated in cancer cells. (a) Western blot analysis of PD-L1 in breast cancer cells. (b) Western blot analysis
Supplementary Figure 1. PD-L1 is glycosylated in cancer cells. (a) Western blot analysis of PD-L1 in breast cancer cells. (b) Western blot analysis of PD-L1 in ovarian cancer cells. (c) Western blot analysis
Supplementary Figure 1 IMQ-Induced Mouse Model of Psoriasis. IMQ cream was
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Supplementary Figure 1
Supplementary Figure 1 how HFD how HFD Epi WT p p Hypothalamus p p Inguinal WT T Liver Lean mouse adipocytes p p p p p p Obese mouse adipocytes Kidney Muscle Spleen Heart p p p p p p p p Extracellular
Supplementary Materials for
advances.sciencemag.org/cgi/content/full/1/8/e1500296/dc1 Supplementary Materials for Transcriptional regulation of APOBEC3 antiviral immunity through the CBF- /RUNX axis This PDF file includes: Brett
Supplementary Information Epigenetic modulation of inflammation and synaptic plasticity promotes resilience against stress in mice
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Supplementary Figures
Supplementary Figures Supplementary Figure 1 Characterization of stable expression of GlucB and sshbira in the CT26 cell line (a) Live cell imaging of stable CT26 cells expressing green fluorescent protein
Supplemental Material for:
Supplemental Material for: Transcriptional silencing of γ-globin by BCL11A involves long-range interactions and cooperation with SOX6 Jian Xu, Vijay G. Sankaran, Min Ni, Tobias F. Menne, Rishi V. Puram,
control kda ATGL ATGLi HSL 82 GAPDH * ** *** WT/cTg WT/cTg ATGLi AKO/cTg AKO/cTg ATGLi WT/cTg WT/cTg ATGLi AKO/cTg AKO/cTg ATGLi iwat gwat ibat
body weight (g) tissue weights (mg) ATGL protein expression (relative to GAPDH) HSL protein expression (relative to GAPDH) ### # # kda ATGL 55 HSL 82 GAPDH 37 2.5 2. 1.5 1..5 2. 1.5 1..5.. Supplementary
Supplementary Materials for. c-abl Activation Plays a Role in α-synucleinopathy Induced Neurodegeneration
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